Rw2G030120

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003486
Physical Location & Seq
Reverse (-)
49234371 .. 49234978
608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030120.1

Sequence Viewer

Length: 351 bp
ATGAACCAAAAAATGAGTGTCCTTAGGATGGTTGAAAAGGTTTGCAAGGATCTTCAAATGCTTGTTGACATATTTGTGAACTATGACTGTGATCTTGAGGCACTGAACTTGTTTGAGCGGATAAATATCTCAAGGGATGCAAAATGCAGATCCAATATGGCTGTTGCGTCCCAGACTAGTTCAATAAAGGGTCCATCTCTTCTGGTAAGTTTAACGATTTACTCCTTCTCAGTTTTGGCTAGTTCATCATATGAAGAGCTTTGTTGCTGCTATATATTCATTGATGGTAGCACAGGTTGCTGGATGACCTCTTTTTCCTTGACCTTGCCAGTGTCTTGTGAATGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

116

Amino Acids

12.93

Weight (kDa)

4.76

Isoelectric Point (pI)

54.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 2 - 36 7.6e-09 Mon2/Sec7/BIG1-like, HUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 118
AciI CCGC 1 cut(s) 118
AclWI GGATC 2 cut(s) 57, 144
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 3 cut(s) 35, 56, 183
AhlI ACTAGT 1 cut(s) 176
AluBI AGCT 1 cut(s) 259
AluI AGCT 1 cut(s) 259
AlwI GGATC 2 cut(s) 57, 144
ApeKI GCWGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 191
AvaII GGWCC 1 cut(s) 191
AxyI CCTNAGG 1 cut(s) 23
BbvI GCAGC 1 cut(s) 254
BccI CCATC 3 cut(s) 22, 202, 278
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 2 cut(s) 177, 240
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 191
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 192
BmsI GCATC 1 cut(s) 127
BpuEI CTTGAG 2 cut(s) 115, 116
BsaBI GATNNNNATC 1 cut(s) 125
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse1I ACTGG 1 cut(s) 329
Bse21I CCTNAGG 1 cut(s) 23
Bse8I GATNNNNATC 1 cut(s) 125
BseGI GGATG 3 cut(s) 33, 142, 309
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 329
BseXI GCAGC 1 cut(s) 254
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 154
Bsp143I GATC 3 cut(s) 49, 91, 149
BspACI CCGC 1 cut(s) 118
BspCNI CTCAG 1 cut(s) 242
BspLI GGNNCC 1 cut(s) 192
BspPI GGATC 2 cut(s) 57, 144
BspQI GCTCTTC 1 cut(s) 249
BsrBI CCGCTC 1 cut(s) 118
BsrI ACTGG 1 cut(s) 329
BssMI GATC 3 cut(s) 49, 91, 149
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 2 cut(s) 204, 249
BstAPI GCANNNNNTGC 1 cut(s) 297
BstDEI CTNAG 2 cut(s) 23, 229
BstF5I GGATG 3 cut(s) 33, 142, 309
BstKTI GATC 3 cut(s) 52, 94, 152
BstMBI GATC 3 cut(s) 49, 91, 149
BstMWI GCNNNNNNNGC 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 254
BstX2I RGATCY 2 cut(s) 49, 149
BstYI RGATCY 2 cut(s) 49, 149
Bsu36I CCTNAGG 1 cut(s) 23
BtsCI GGATG 3 cut(s) 33, 142, 309
BtsIMutI CAGTG 2 cut(s) 101, 336
Cfr13I GGNCC 1 cut(s) 191
CseI GACGC 1 cut(s) 156
CviJI RGCY 3 cut(s) 161, 239, 259
CviKI_1 RGCY 3 cut(s) 161, 239, 259
DdeI CTNAG 2 cut(s) 23, 229
DpnI GATC 3 cut(s) 51, 93, 151
DpnII GATC 3 cut(s) 49, 91, 149
Eam1104I CTCTTC 2 cut(s) 204, 249
EarI CTCTTC 2 cut(s) 204, 249
Eco47I GGWCC 1 cut(s) 191
Eco81I CCTNAGG 1 cut(s) 23
FaiI YATR 7 cut(s) 71, 84, 158, 250, 252, 273, 275
FaqI GGGAC 1 cut(s) 154
FauNDI CATATG 1 cut(s) 250
Fnu4HI GCNGC 1 cut(s) 268
FokI GGATG 3 cut(s) 40, 149, 316
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 2 cut(s) 177, 240
GluI GCNGC 1 cut(s) 268
HgaI GACGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
Hpy166II GTNNAC 2 cut(s) 67, 79
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 2 cut(s) 67, 79
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4V TGCA 3 cut(s) 45, 140, 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 297
HpyF3I CTNAG 2 cut(s) 23, 229
Kzo9I GATC 3 cut(s) 49, 91, 149
LguI GCTCTTC 1 cut(s) 249
LpnPI CCDG 5 cut(s) 185, 188, 279, 286, 342
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 177, 240
MalI GATC 3 cut(s) 51, 93, 151
MbiI CCGCTC 1 cut(s) 118
MboI GATC 3 cut(s) 49, 91, 149
MboII GAAGA 3 cut(s) 44, 191, 266
MflI RGATCY 2 cut(s) 49, 149
MnlI CCTC 2 cut(s) 91, 319
MseI TTAA 2 cut(s) 212, 349
MslI CAYNNNNRTG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 297
NdeI CATATG 1 cut(s) 250
NdeII GATC 3 cut(s) 49, 91, 149
NlaIV GGNNCC 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 249
PkrI GCNGC 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 1 cut(s) 191
PsuI RGATCY 2 cut(s) 49, 149
RseI CAYNNNNRTG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 212, 349
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 3 cut(s) 49, 91, 149
Sau96I GGNCC 1 cut(s) 191
SetI ASST 5 cut(s) 42, 261, 298, 311, 326
SfaNI GCATC 1 cut(s) 127
SinI GGWCC 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 95, 130
SmoI CTYRAG 2 cut(s) 95, 130
SpeI ACTAGT 1 cut(s) 176
SsiI CCGC 1 cut(s) 118
SspMI CTAG 2 cut(s) 177, 240
TaaI ACNGT 1 cut(s) 89
Tru1I TTAA 2 cut(s) 212, 349
Tru9I TTAA 2 cut(s) 212, 349
TscAI CASTG 2 cut(s) 108, 336
TseI GCWGC 1 cut(s) 267
TspDTI ATGAA 4 cut(s) 17, 234, 267, 268
TspRI CASTG 2 cut(s) 108, 336
VpaK11BI GGWCC 1 cut(s) 191
XspI CTAG 2 cut(s) 177, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.