RLG00000008535

Brefeldin A-inhibited guanine nucleotide-exchange protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003486
Physical Location & Seq
Reverse (-)
31741047 .. 31742579
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008535

Sequence Viewer

Length: 459 bp
ATGAAGGAAGATAACAATGAAGTCACTTTGAAGACGAGGATTTTATCTCTTGAGCTTCTGCAGGGTGAAATATGCATCTTCTTTCCTTTGATATTTCTCCGGTCCCTTGATGGCTTGGATTTACCCATGAACCAAAAAATGAGTGTCCTTAGGATGGTTGAAAAGGTTTGCAAGGATCTTCAGATGCTTGTTGACATATTTGTGAACTATGACTGTGATCTTGAGGCACCGAACTTGTTTGAGCGGATGAATATCTCAAGGGATGCAAAATGCAGATCCAATATGGCTGTTGCATCCCAGACTACTTTAATAAAGGGTTCATCTCTTCTGTCTCTGGAAGGAGATGCTTCAGCTAAAGAGTGTGTTGATGTGAAAAGCAGGCAAGATGCAACCACTAACTTTGAGAAGGCAAAAGCTCATAAGTCTACAGTGGAATCTGCTATATCTGAGGTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0002376 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0006887 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007030 GO:0008064 GO:0008092 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010256 GO:0010556 GO:0010557 GO:0010559 GO:0010560 GO:0010604 GO:0010639 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016363 GO:0017022 GO:0019222 GO:0019899 GO:0030532 GO:0030832 GO:0030833 GO:0030837 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031333 GO:0031399 GO:0031401 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032268 GO:0032270 GO:0032271 GO:0032272 GO:0032535 GO:0032878 GO:0032940 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0034237 GO:0034260 GO:0034399 GO:0040007 GO:0042742 GO:0043086 GO:0043087 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043254 GO:0044087 GO:0044092 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046903 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051018 GO:0051020 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051336 GO:0051346 GO:0051493 GO:0051494 GO:0051704 GO:0051707 GO:0060049 GO:0060050 GO:0060255 GO:0061041 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071840 GO:0080090 GO:0080134 GO:0090066 GO:0090283 GO:0090284 GO:0090303 GO:0097708 GO:0098542 GO:0098588 GO:0098772 GO:0098791 GO:0110053 GO:0120114 GO:1902903 GO:1902904 GO:1903018 GO:1903020 GO:1903034 GO:1903036 GO:1990904 GO:2000112 GO:2000114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DCB DUF1981 Mon2_C Sec7 Sec7_N

Protein Analysis

153

Amino Acids

17.07

Weight (kDa)

5.03

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec7-like_HUS PF12783 18 - 79 1.5e-14 Mon2/Sec7/BIG1-like, HUS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 226
AccBSI CCGCTC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 425
AciI CCGC 1 cut(s) 244
AclWI GGATC 2 cut(s) 183, 270
AcuI CTGAAG 2 cut(s) 164, 333
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 2 cut(s) 31, 161
AluBI AGCT 3 cut(s) 55, 353, 416
AluI AGCT 3 cut(s) 55, 353, 416
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 2 cut(s) 183, 270
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 77
AvaII GGWCC 1 cut(s) 102
AxyI CCTNAGG 1 cut(s) 149
BanI GGYRCC 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 38
BccI CCATC 2 cut(s) 104, 148
BcoDI GTCTC 1 cut(s) 336
BfmI CTRYAG 2 cut(s) 59, 426
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 2 cut(s) 104, 228
BmsI GCATC 6 cut(s) 84, 174, 253, 302, 334, 376
BpiI GAAGAC 1 cut(s) 38
BpuEI CTTGAG 3 cut(s) 71, 241, 242
BsaBI GATNNNNATC 1 cut(s) 251
BsaWI WCCGGW 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 154
Bse21I CCTNAGG 1 cut(s) 149
Bse8I GATNNNNATC 1 cut(s) 251
BseGI GGATG 4 cut(s) 159, 252, 268, 293
BseJI GATNNNNATC 1 cut(s) 251
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 438
BshNI GGYRCC 1 cut(s) 226
BsiSI CCGG 1 cut(s) 100
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 88
Bsp143I GATC 3 cut(s) 175, 217, 275
BspACI CCGC 1 cut(s) 244
BspCNI CTCAG 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 104, 228
BspMAI CTGCAG 1 cut(s) 63
BspPI GGATC 2 cut(s) 183, 270
BspT107I GGYRCC 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 244
BssMI GATC 3 cut(s) 175, 217, 275
Bst4CI ACNGT 2 cut(s) 215, 430
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 380
BstDEI CTNAG 2 cut(s) 149, 447
BstF5I GGATG 4 cut(s) 159, 252, 268, 293
BstKTI GATC 3 cut(s) 178, 220, 278
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 3 cut(s) 175, 217, 275
BstSFI CTRYAG 2 cut(s) 59, 426
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 175, 275
BstYI RGATCY 2 cut(s) 175, 275
Bsu36I CCTNAGG 1 cut(s) 149
BtsCI GGATG 4 cut(s) 159, 252, 268, 293
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 102
CspCI CAANNNNNGTGG 2 cut(s) 382, 417
CviAII CATG 1 cut(s) 127
CviJI RGCY 5 cut(s) 55, 114, 287, 353, 416
CviKI_1 RGCY 5 cut(s) 55, 114, 287, 353, 416
DdeI CTNAG 2 cut(s) 149, 447
DpnI GATC 3 cut(s) 177, 219, 277
DpnII GATC 3 cut(s) 175, 217, 275
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 2 cut(s) 164, 333
Eco81I CCTNAGG 1 cut(s) 149
EcoT22I ATGCAT 1 cut(s) 77
FaeI CATG 1 cut(s) 130
FaiI YATR 7 cut(s) 73, 128, 197, 210, 284, 420, 443
FaqI GGGAC 1 cut(s) 88
FatI CATG 1 cut(s) 126
FblI GTMKAC 1 cut(s) 425
FokI GGATG 4 cut(s) 166, 259, 275, 280
HapII CCGG 1 cut(s) 100
Hin1II CATG 1 cut(s) 130
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 1 cut(s) 434
HpaII CCGG 1 cut(s) 100
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 193, 205, 426
Hpy188I TCNGA 2 cut(s) 183, 448
Hpy188III TCNNGA 3 cut(s) 50, 221, 335
Hpy8I GTNNAC 3 cut(s) 193, 205, 426
HpyAV CCTTC 2 cut(s) 332, 400
HpyCH4III ACNGT 2 cut(s) 215, 430
HpyCH4V TGCA 7 cut(s) 61, 75, 171, 266, 273, 293, 389
HpyF3I CTNAG 2 cut(s) 149, 447
Hsp92II CATG 1 cut(s) 130
Kzo9I GATC 3 cut(s) 175, 217, 275
LpnPI CCDG 5 cut(s) 47, 113, 311, 320, 364
LweI GCATC 6 cut(s) 84, 174, 253, 302, 334, 376
MaeIII GTNAC 1 cut(s) 22
MalI GATC 3 cut(s) 177, 219, 277
MbiI CCGCTC 1 cut(s) 244
MboI GATC 3 cut(s) 175, 217, 275
MboII GAAGA 5 cut(s) 20, 43, 70, 170, 317
MflI RGATCY 2 cut(s) 175, 275
MnlI CCTC 3 cut(s) 30, 217, 442
Mph1103I ATGCAT 1 cut(s) 77
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 1 cut(s) 100
NdeII GATC 3 cut(s) 175, 217, 275
NlaIII CATG 1 cut(s) 130
NlaIV GGNNCC 2 cut(s) 104, 228
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 77
PfeI GAWTC 1 cut(s) 434
PspN4I GGNNCC 2 cut(s) 104, 228
PspPI GGNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 2 cut(s) 175, 275
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 308
Sau3AI GATC 3 cut(s) 175, 217, 275
Sau96I GGNCC 1 cut(s) 102
SetI ASST 5 cut(s) 57, 168, 355, 418, 453
SfaNI GCATC 6 cut(s) 84, 174, 253, 302, 334, 376
SfcI CTRYAG 2 cut(s) 59, 426
SinI GGWCC 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 50, 221, 256
SmoI CTYRAG 3 cut(s) 50, 221, 256
SsiI CCGC 1 cut(s) 244
TaaI ACNGT 2 cut(s) 215, 430
TfiI GAWTC 1 cut(s) 434
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 5 cut(s) 17, 33, 143, 263, 309
TspRI CASTG 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.