RchiOBHm_Chr2g0136401

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004345
Physical Location & Seq
Forward (+)
53811409 .. 53811971
563 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50719

Sequence Viewer

Length: 297 bp
ATGAAGAAGAGCTCCATGCCTCCTCATCCTTTATACTCTCCCATTCTCGAAGAGCCTGAGAAACAAGAAGCCAAAAGCTTTAGGGTACTCGAGAAAGGACCTGCTGCAAGGTCTCATGCTGATCTGGATCAAAACAAAGAGGCAGCAGCTGCAGCACCGAAGAAGCTAGAGAGGAAGACAACCGAAGACATCAATGAAAGCGCCGATGCATTCATCAAGAAGTTCAGAAAGCAGCTGATGATCCAAAGGCTAGAGTCGATCGAGAATTACGAGCAAATGCTAGCAAGGGGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

98

Amino Acids

11.19

Weight (kDa)

8.89

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 62 - 96 6.7e-17 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 109
AclWI GGATC 2 cut(s) 135, 235
AfaI GTAC 1 cut(s) 87
AluBI AGCT 5 cut(s) 12, 78, 149, 166, 235
AluI AGCT 5 cut(s) 12, 78, 149, 166, 235
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 117
AlwI GGATC 2 cut(s) 135, 235
AlwNI CAGNNNCTG 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 89
ApeKI GCWGC 6 cut(s) 104, 143, 146, 149, 152, 232
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 98
AsuNHI GCTAGC 1 cut(s) 280
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 1 cut(s) 98
BanII GRGCYC 2 cut(s) 14, 294
BbsI GAAGAC 2 cut(s) 182, 192
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 6 cut(s) 91, 136, 155, 158, 164, 244
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 3 cut(s) 167, 251, 281
BfmI CTRYAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 6 cut(s) 105, 144, 147, 150, 153, 233
BlsI GCNGC 6 cut(s) 106, 145, 148, 151, 154, 234
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 98
BmsI GCATC 1 cut(s) 196
BmtI GCTAGC 1 cut(s) 284
BpiI GAAGAC 2 cut(s) 182, 192
BsaBI GATNNNNATC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 117
Bse8I GATNNNNATC 1 cut(s) 126
BseGI GGATG 1 cut(s) 25
BseJI GATNNNNATC 1 cut(s) 126
BseMII CTCAG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 12
BseXI GCAGC 6 cut(s) 91, 136, 155, 158, 164, 244
Bsh1285I CGRYCG 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 89
BsmAI GTCTC 1 cut(s) 117
BsmI GAATGC 1 cut(s) 209
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 14, 294
Bsp143I GATC 4 cut(s) 121, 127, 240, 258
BspCNI CTCAG 1 cut(s) 49
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 284
BspPI GGATC 2 cut(s) 135, 235
BspQI GCTCTTC 2 cut(s) 2, 45
BspTNI GGTCTC 1 cut(s) 117
BssMI GATC 4 cut(s) 121, 127, 240, 258
Bst6I CTCTTC 2 cut(s) 2, 45
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 4 cut(s) 124, 130, 243, 261
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 4 cut(s) 121, 127, 240, 258
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 149, 152
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 6 cut(s) 91, 136, 155, 158, 164, 244
BstV2I GAAGAC 2 cut(s) 182, 192
BtsCI GGATG 1 cut(s) 25
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 86
CviAII CATG 2 cut(s) 16, 116
CviJI RGCY 9 cut(s) 12, 55, 71, 78, 149, 166, 235, 250, 292
CviKI_1 RGCY 9 cut(s) 12, 55, 71, 78, 149, 166, 235, 250, 292
CviQI GTAC 1 cut(s) 86
DdeI CTNAG 1 cut(s) 57
DpnI GATC 4 cut(s) 123, 129, 242, 260
DpnII GATC 4 cut(s) 121, 127, 240, 258
Eam1104I CTCTTC 2 cut(s) 2, 45
EarI CTCTTC 2 cut(s) 2, 45
Ecl136II GAGCTC 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 14, 294
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 98
EcoT22I ATGCAT 1 cut(s) 211
EcoT38I GRGCYC 2 cut(s) 14, 294
FaeI CATG 2 cut(s) 19, 119
FaiI YATR 3 cut(s) 17, 34, 117
FatI CATG 2 cut(s) 15, 115
Fnu4HI GCNGC 6 cut(s) 105, 144, 147, 150, 153, 233
FokI GGATG 1 cut(s) 12
FriOI GRGCYC 2 cut(s) 14, 294
Fsp4HI GCNGC 6 cut(s) 105, 144, 147, 150, 153, 233
FspBI CTAG 3 cut(s) 167, 251, 281
GlaI GCGC 1 cut(s) 202
GluI GCNGC 6 cut(s) 105, 144, 147, 150, 153, 233
HaeII RGCGCY 1 cut(s) 204
HhaI GCGC 1 cut(s) 203
Hin1II CATG 2 cut(s) 19, 119
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 1 cut(s) 254
Hpy188I TCNGA 1 cut(s) 227
Hpy188III TCNNGA 5 cut(s) 47, 91, 125, 217, 262
HpyCH4V TGCA 3 cut(s) 107, 152, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 152
HpyF3I CTNAG 1 cut(s) 57
Hsp92II CATG 2 cut(s) 19, 119
HspAI GCGC 1 cut(s) 201
Kzo9I GATC 4 cut(s) 121, 127, 240, 258
LguI GCTCTTC 2 cut(s) 2, 45
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 3 cut(s) 69, 110, 114
Lsp1109I GCAGC 6 cut(s) 91, 136, 155, 158, 164, 244
LweI GCATC 1 cut(s) 196
MaeI CTAG 3 cut(s) 167, 251, 281
MalI GATC 4 cut(s) 123, 129, 242, 260
MboI GATC 4 cut(s) 121, 127, 240, 258
MboII GAAGA 6 cut(s) 16, 19, 62, 172, 187, 197
MhlI GDGCHC 2 cut(s) 14, 294
MluCI AATT 1 cut(s) 265
MlyI GAGTC 1 cut(s) 263
MnlI CCTC 4 cut(s) 30, 33, 133, 165
Mph1103I ATGCAT 1 cut(s) 211
MspA1I CMGCKG 2 cut(s) 149, 235
Mva1269I GAATGC 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 149, 152
NdeII GATC 4 cut(s) 121, 127, 240, 258
NheI GCTAGC 1 cut(s) 280
NlaIII CATG 2 cut(s) 19, 119
NsiI ATGCAT 1 cut(s) 211
PaeR7I CTCGAG 1 cut(s) 89
PciSI GCTCTTC 2 cut(s) 2, 45
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 209
PkrI GCNGC 6 cut(s) 106, 145, 148, 151, 154, 234
Ple19I CGATCG 1 cut(s) 261
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
PpuMI RGGWCCY 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 98
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 149
PvuI CGATCG 1 cut(s) 261
PvuII CAGCTG 2 cut(s) 149, 235
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 14
SapI GCTCTTC 2 cut(s) 2, 45
SatI GCNGC 6 cut(s) 105, 144, 147, 150, 153, 233
Sau3AI GATC 4 cut(s) 121, 127, 240, 258
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 1 cut(s) 263
SduI GDGCHC 2 cut(s) 14, 294
SetI ASST 7 cut(s) 14, 80, 103, 113, 151, 168, 237
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 1 cut(s) 150
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 1 cut(s) 98
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 1 cut(s) 265
SspMI CTAG 3 cut(s) 167, 251, 281
SstI GAGCTC 1 cut(s) 14
TaqI TCGA 4 cut(s) 48, 90, 257, 261
TasI AATT 1 cut(s) 265
TseI GCWGC 6 cut(s) 104, 143, 146, 149, 152, 232
TspDTI ATGAA 3 cut(s) 17, 202, 210
VpaK11BI GGWCC 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 89
XspI CTAG 3 cut(s) 167, 251, 281
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.