RchiOBHm_Chr2g0165441

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002507
Physical Location & Seq
Reverse (-)
80388009 .. 80389999
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53339

Sequence Viewer

Length: 690 bp
ATGGAAAGGTCACTGAGCATGTCCTCCCTTCATTCAAACAGATCAATAGGTTCGAAGGAGTGGAATATTGGGTTCTCGGACCAGAGGCAGCTATTTCTTACCATATCTAATGTTTTAAAAGAACGTAAAAGCTTGGCAAAGGAGTCTATCAAGTTCCTAACTAAGTACTTGGCAACATTTTCTGGCGAAGATGCACATGTTTTGAGCCAAGCCAAGGTCAGCTGTTTCAAACCTCAAGATAATTGGCATTTGGCTCAGATGTTCCTCGTCTTTCTAGTAGGATTGTCTTTTGCGTGTGATTTGTTAGATATGCCTGTTGTAGCGCAGTCGGAGAAGGATGCTAAACATGCATTGGCATATCAGCTTTTGAAGGTATTTTTGACCCAGAGGCTGGATGCGTACTTGGAGTTTCAGGCAGCAAATTCTACTCTGCTACAAAGCTGTGGTCTGGTCCATGAAGATTGTATTACTAAAATGAGGTTGATTTCATTGGTGGATCTGGGCTGTGATGAATCTGGACGAATTCCTTATAGTCTCACTAGAGATACACTTCAGATTAATGATGATGAAGTGGAACCTTGGGTAGTTAAGGCTATAACAGCCAAGTTATTGGACTGGAAGATGGACCAGATGAATGAAGTTGTAGTTGTAACCCGCTCTACTCAGTGTGTGTTTGGCAAGGAGCAATGA

Protein Analysis

229

Amino Acids

25.96

Weight (kDa)

5.97

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCI PF01399 141 - 218 7.9e-09 PCI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 657
AciI CCGC 1 cut(s) 655
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 2 cut(s) 421, 522
AcuI CTGAAG 1 cut(s) 536
AfaI GTAC 2 cut(s) 167, 401
AfiI CCNNNNNNNGG 2 cut(s) 214, 391
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 3 cut(s) 36, 229, 370
AluBI AGCT 5 cut(s) 91, 132, 222, 364, 441
AluI AGCT 5 cut(s) 91, 132, 222, 364, 441
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 1 cut(s) 504
AlwNI CAGNNNCTG 1 cut(s) 391
ApeKI GCWGC 2 cut(s) 88, 416
ApoI RAATTY 2 cut(s) 421, 522
ArsI GACNNNNNNTTYG 2 cut(s) 430, 462
AseI ATTAAT 1 cut(s) 558
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 3 cut(s) 79, 451, 625
AsuII TTCGAA 1 cut(s) 53
AvaII GGWCC 3 cut(s) 79, 451, 625
BbvI GCAGC 2 cut(s) 100, 428
BccI CCATC 1 cut(s) 616
BcoDI GTCTC 1 cut(s) 539
BfaI CTAG 2 cut(s) 275, 540
BisI GCNGC 2 cut(s) 89, 417
BlsI GCNGC 2 cut(s) 90, 418
BmcAI AGTACT 1 cut(s) 167
Bme18I GGWCC 3 cut(s) 79, 451, 625
BmgT120I GGNCC 3 cut(s) 79, 451, 625
BmiI GGNNCC 1 cut(s) 576
BmsI GCATC 3 cut(s) 181, 328, 385
Bpu14I TTCGAA 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 213, 578
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 391
Bse1I ACTGG 1 cut(s) 620
BseDI CCNNGG 2 cut(s) 213, 578
BseGI GGATG 2 cut(s) 343, 400
BseLI CCNNNNNNNGG 2 cut(s) 214, 391
BseMII CTCAG 3 cut(s) 5, 269, 677
BseNI ACTGG 1 cut(s) 620
BseXI GCAGC 2 cut(s) 100, 428
BslI CCNNNNNNNGG 2 cut(s) 214, 391
BsmAI GTCTC 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 53
Bsp143I GATC 2 cut(s) 41, 496
BspACI CCGC 1 cut(s) 655
BspCNI CTCAG 3 cut(s) 6, 268, 676
BspLI GGNNCC 1 cut(s) 576
BspPI GGATC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 657
BsrI ACTGG 1 cut(s) 620
BssECI CCNNGG 2 cut(s) 213, 578
BssMI GATC 2 cut(s) 41, 496
BssT1I CCWWGG 2 cut(s) 213, 578
BstBI TTCGAA 1 cut(s) 53
BstDEI CTNAG 4 cut(s) 14, 162, 255, 663
BstF5I GGATG 2 cut(s) 343, 400
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 2 cut(s) 44, 499
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 2 cut(s) 41, 496
BstMWI GCNNNNNNNGC 2 cut(s) 347, 599
BstNSI RCATGY 3 cut(s) 22, 200, 350
BstV1I GCAGC 2 cut(s) 100, 428
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 610
BstYI RGATCY 1 cut(s) 496
BtsCI GGATG 2 cut(s) 343, 400
BtsIMutI CAGTG 2 cut(s) 11, 671
CaiI CAGNNNCTG 1 cut(s) 391
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 3 cut(s) 79, 451, 625
Csp6I GTAC 2 cut(s) 166, 400
CviAII CATG 4 cut(s) 19, 197, 347, 455
CviQI GTAC 2 cut(s) 166, 400
DdeI CTNAG 4 cut(s) 14, 162, 255, 663
DpnI GATC 2 cut(s) 43, 498
DpnII GATC 2 cut(s) 41, 496
DraI TTTAAA 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 213, 578
Eco47I GGWCC 3 cut(s) 79, 451, 625
Eco57I CTGAAG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 522
EcoT14I CCWWGG 2 cut(s) 213, 578
EcoT22I ATGCAT 1 cut(s) 352
ErhI CCWWGG 2 cut(s) 213, 578
FaeI CATG 4 cut(s) 22, 200, 350, 458
FaiI YATR 9 cut(s) 20, 104, 198, 311, 348, 358, 456, 531, 596
FatI CATG 4 cut(s) 18, 196, 346, 454
FauI CCCGC 1 cut(s) 662
Fnu4HI GCNGC 2 cut(s) 89, 417
FokI GGATG 2 cut(s) 350, 407
Fsp4HI GCNGC 2 cut(s) 89, 417
FspBI CTAG 2 cut(s) 275, 540
GlaI GCGC 1 cut(s) 324
GluI GCNGC 2 cut(s) 89, 417
HhaI GCGC 1 cut(s) 325
Hin1II CATG 4 cut(s) 22, 200, 350, 458
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HindIII AAGCTT 1 cut(s) 130
HinfI GANTC 2 cut(s) 143, 512
Hpy188I TCNGA 4 cut(s) 79, 258, 331, 555
Hpy188III TCNNGA 2 cut(s) 236, 516
HpyAV CCTTC 4 cut(s) 38, 49, 328, 364
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 2 cut(s) 194, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 599
HpyF3I CTNAG 4 cut(s) 14, 162, 255, 663
HpySE526I ACGT 1 cut(s) 124
Hsp92II CATG 4 cut(s) 22, 200, 350, 458
HspAI GCGC 1 cut(s) 323
Kzo9I GATC 2 cut(s) 41, 496
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 2 cut(s) 100, 428
LweI GCATC 3 cut(s) 181, 328, 385
MaeI CTAG 2 cut(s) 275, 540
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 9, 649
MalI GATC 2 cut(s) 43, 498
MbiI CCGCTC 1 cut(s) 657
MboI GATC 2 cut(s) 41, 496
MboII GAAGA 3 cut(s) 200, 470, 631
MflI RGATCY 1 cut(s) 496
MluCI AATT 3 cut(s) 241, 421, 522
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 6 cut(s) 34, 78, 243, 275, 381, 471
Mph1103I ATGCAT 1 cut(s) 352
MseI TTAA 3 cut(s) 116, 558, 588
MspA1I CMGCKG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 347, 599
NdeII GATC 2 cut(s) 41, 496
NlaIII CATG 4 cut(s) 22, 200, 350, 458
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 9
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 3 cut(s) 22, 200, 350
NspV TTCGAA 1 cut(s) 53
PciI ACATGT 1 cut(s) 196
PfeI GAWTC 1 cut(s) 512
PflMI CCANNNNNTGG 1 cut(s) 391
PkrI GCNGC 2 cut(s) 90, 418
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PscI ACATGT 1 cut(s) 196
PshBI ATTAAT 1 cut(s) 558
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 3 cut(s) 79, 451, 625
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 222
RsaI GTAC 2 cut(s) 167, 401
RsaNI GTAC 2 cut(s) 166, 400
SaqAI TTAA 3 cut(s) 116, 558, 588
SatI GCNGC 2 cut(s) 89, 417
Sau3AI GATC 2 cut(s) 41, 496
Sau96I GGNCC 3 cut(s) 79, 451, 625
ScaI AGTACT 1 cut(s) 167
SchI GAGTC 1 cut(s) 152
SfaNI GCATC 3 cut(s) 181, 328, 385
SfuI TTCGAA 1 cut(s) 53
SinI GGWCC 3 cut(s) 79, 451, 625
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
Sse9I AATT 3 cut(s) 241, 421, 522
SsiI CCGC 1 cut(s) 655
SspI AATATT 1 cut(s) 67
SspMI CTAG 2 cut(s) 275, 540
StyI CCWWGG 2 cut(s) 213, 578
TaiI ACGT 1 cut(s) 127
TaqI TCGA 1 cut(s) 53
TasI AATT 3 cut(s) 241, 421, 522
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 3 cut(s) 116, 558, 588
Tru9I TTAA 3 cut(s) 116, 558, 588
TscAI CASTG 2 cut(s) 18, 671
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 2 cut(s) 88, 416
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 7 cut(s) 20, 471, 477, 525, 582, 647, 651
TspRI CASTG 2 cut(s) 18, 671
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 3 cut(s) 79, 451, 625
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 2 cut(s) 421, 522
XceI RCATGY 3 cut(s) 22, 200, 350
XspI CTAG 2 cut(s) 275, 540
ZrmI AGTACT 1 cut(s) 167
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.