Rroxscaffold_6G00392200

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002507
Physical Location & Seq
Reverse (-)
11598378 .. 11603095
4718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00392200.1

Sequence Viewer

Length: 396 bp
ATGCCTGCTGTAGCGCAGTTGGAGAAGGATGCTAAACATGCATTGGCATATCAAGTTTTGAAGGTATTTTTGACCCAGAGGCTGGATGTGTACTTGGAGTTTCAGGCAGCAAATTCTACTCTGCTACAAAGCTGTGGTCTGGTCCATGAAGAGTGTATTACTAAGATGAGGTTGATTTCATTGATTAATGATGATGAAGTGGAACCTTGGGTGGTTAAGGCTATAACAGCCAAGTTATTGGTCTGTAGGATGGACCAGATGAATGAAGTTGTAGTAGTAACCCGCTCTACTCAGCGTGTGTTTGGCAAGGAGCAATGGCATACTTTTAAGACAAAGCTAGCATCTTTGAGGAAAAAGAGTTCGTGGCTTAAGCGAAGAACAGGAAGCTCAAATTAG

Protein Analysis

131

Amino Acids

15.04

Weight (kDa)

9.55

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCI PF01399 63 - 94 1e-05 PCI domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 82
AccBSI CCGCTC 1 cut(s) 285
AciI CCGC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 112
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 82
AflII CTTAAG 1 cut(s) 368
AgsI TTSAA 1 cut(s) 61
AloI GAACNNNNNNTCC 2 cut(s) 343, 375
AluBI AGCT 3 cut(s) 132, 337, 387
AluI AGCT 3 cut(s) 132, 337, 387
AlwNI CAGNNNCTG 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 112
ArsI GACNNNNNNTTYG 2 cut(s) 121, 153
AseI ATTAAT 1 cut(s) 186
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 2 cut(s) 142, 253
AsuNHI GCTAGC 1 cut(s) 337
AvaII GGWCC 2 cut(s) 142, 253
BbvI GCAGC 1 cut(s) 119
BccI CCATC 1 cut(s) 244
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 2 cut(s) 9, 244
BfrI CTTAAG 1 cut(s) 368
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme18I GGWCC 2 cut(s) 142, 253
BmgT120I GGNCC 2 cut(s) 142, 253
BmiI GGNNCC 1 cut(s) 204
BmsI GCATC 2 cut(s) 19, 350
BmtI GCTAGC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse3DI GCAATG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 206
BseGI GGATG 3 cut(s) 34, 91, 255
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 305
BseXI GCAGC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 82
BspACI CCGC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 204
BspOI GCTAGC 1 cut(s) 341
BspTI CTTAAG 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 206
Bst6I CTCTTC 1 cut(s) 144
BstAFI CTTAAG 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 6, 339
BstDEI CTNAG 2 cut(s) 162, 291
BstF5I GGATG 3 cut(s) 34, 91, 255
BstHHI GCGC 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 38, 227
BstNSI RCATGY 1 cut(s) 41
BstSFI CTRYAG 2 cut(s) 9, 244
BstV1I GCAGC 1 cut(s) 119
BstXI CCANNNNNNTGG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 34, 91, 255
Cac8I GCNNGC 2 cut(s) 6, 339
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 142, 253
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 38, 146
CviJI RGCY 7 cut(s) 82, 132, 221, 230, 337, 367, 387
CviKI_1 RGCY 7 cut(s) 82, 132, 221, 230, 337, 367, 387
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 2 cut(s) 162, 291
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 206
Eco47I GGWCC 2 cut(s) 142, 253
EcoT14I CCWWGG 1 cut(s) 206
EcoT22I ATGCAT 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 2 cut(s) 41, 149
FaiI YATR 5 cut(s) 39, 49, 147, 224, 321
FatI CATG 2 cut(s) 37, 145
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 3 cut(s) 41, 98, 262
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 338
GlaI GCGC 1 cut(s) 15
GluI GCNGC 1 cut(s) 108
HhaI GCGC 1 cut(s) 16
Hin1II CATG 2 cut(s) 41, 149
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
Hpy166II GTNNAC 1 cut(s) 91
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 2 cut(s) 19, 55
HpyCH4V TGCA 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 227
HpyF3I CTNAG 2 cut(s) 162, 291
Hsp92II CATG 2 cut(s) 41, 149
HspAI GCGC 1 cut(s) 14
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 18, 68, 89, 89, 125, 269, 366
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 2 cut(s) 19, 350
MaeI CTAG 1 cut(s) 338
MaeIII GTNAC 1 cut(s) 277
MbiI CCGCTC 1 cut(s) 285
MboII GAAGA 2 cut(s) 161, 387
MluCI AATT 2 cut(s) 112, 391
MnlI CCTC 3 cut(s) 72, 162, 342
Mph1103I ATGCAT 1 cut(s) 43
MseI TTAA 4 cut(s) 186, 216, 327, 369
MspCI CTTAAG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 38, 227
NheI GCTAGC 1 cut(s) 337
NlaIII CATG 2 cut(s) 41, 149
NlaIV GGNNCC 1 cut(s) 204
NsiI ATGCAT 1 cut(s) 43
NspI RCATGY 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 1 cut(s) 109
PshBI ATTAAT 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 2 cut(s) 142, 253
PstNI CAGNNNCTG 1 cut(s) 82
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SaqAI TTAA 4 cut(s) 186, 216, 327, 369
SatI GCNGC 1 cut(s) 108
Sau96I GGNCC 2 cut(s) 142, 253
SetI ASST 6 cut(s) 66, 134, 173, 208, 339, 389
SfaNI GCATC 2 cut(s) 19, 350
SfcI CTRYAG 2 cut(s) 9, 244
SinI GGWCC 2 cut(s) 142, 253
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 112, 391
SsiI CCGC 1 cut(s) 283
SspMI CTAG 1 cut(s) 338
StyI CCWWGG 1 cut(s) 206
TasI AATT 2 cut(s) 112, 391
TatI WGTACW 1 cut(s) 90
Tru1I TTAA 4 cut(s) 186, 216, 327, 369
Tru9I TTAA 4 cut(s) 186, 216, 327, 369
TseI GCWGC 1 cut(s) 107
TspDTI ATGAA 5 cut(s) 162, 168, 210, 275, 279
Van91I CCANNNNNTGG 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 2 cut(s) 142, 253
VspI ATTAAT 1 cut(s) 186
XapI RAATTY 1 cut(s) 112
XceI RCATGY 1 cut(s) 41
XspI CTAG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.