Rh5DG568600

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002507
Physical Location & Seq
Reverse (-)
87525180 .. 87525515
336 bp
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UTR
Exon/CDS
Intron
Rh5DG568600.1

Sequence Viewer

Length: 255 bp
ATGAGGTTGATTTCATTGGTGGATCTTGGCTCTGATGAATCTGGACGAGTTCCTTATAGTCTCATCAGAGATACACTGCAGATTAATGATGATGAAGTGGAACCTTGGGTGCGTGTGTTCGGCAAGGAGCAATGGCATACCCTTAAAACAAAGCTAGCATGTTGGAGGGGCAATATTTCACATGTCATCAGCACTGTTCAAGCTAACAGGCCAGCTGAAGATGGTGCCCAGGCAGCTCAATACTTGGTTCGTTAG

Protein Analysis

84

Amino Acids

9.55

Weight (kDa)

6.05

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3m_C_helix PF18005 38 - 66 1.8e-11 eIF3 subunit M, C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 224
AclWI GGATC 1 cut(s) 30
AcuI CTGAAG 1 cut(s) 237
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 1 cut(s) 200
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 4 cut(s) 154, 203, 215, 236
AluI AGCT 4 cut(s) 154, 203, 215, 236
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 30
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 233
AseI ATTAAT 1 cut(s) 84
AsuNHI GCTAGC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 229
BanI GGYRCC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 245
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 230
BmiI GGNNCC 2 cut(s) 102, 226
BmrFI CCNGG 1 cut(s) 230
BmtI GCTAGC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 104, 228
Bse3DI GCAATG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 104, 228
BseMI GCAATG 1 cut(s) 137
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 1 cut(s) 245
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 65
BsnI GGCC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 1 cut(s) 22
BspANI GGCC 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 102, 226
BspMAI CTGCAG 1 cut(s) 81
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 224
BsrDI GCAATG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 104, 228
BssMI GATC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 196
BstC8I GCNNGC 2 cut(s) 156, 213
BstKTI GATC 1 cut(s) 25
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 2 cut(s) 162, 185
BstSCI CCNGG 1 cut(s) 228
BstSFI CTRYAG 1 cut(s) 77
BstSLI GKGCMC 1 cut(s) 229
BstV1I GCAGC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BsuRI GGCC 1 cut(s) 211
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 2 cut(s) 74, 192
Cac8I GCNNGC 2 cut(s) 156, 213
CviAII CATG 2 cut(s) 159, 182
CviJI RGCY 6 cut(s) 30, 154, 203, 211, 215, 236
CviKI_1 RGCY 6 cut(s) 30, 154, 203, 211, 215, 236
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 104
FaeI CATG 2 cut(s) 162, 185
FaiI YATR 4 cut(s) 57, 138, 160, 183
FatI CATG 2 cut(s) 158, 181
Fnu4HI GCNGC 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 155
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 1 cut(s) 211
Hin1II CATG 2 cut(s) 162, 185
HinfI GANTC 1 cut(s) 38
Hpy188I TCNGA 2 cut(s) 34, 68
Hpy188III TCNNGA 1 cut(s) 42
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 1 cut(s) 79
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
Hsp92II CATG 2 cut(s) 162, 185
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 127
LpnPI CCDG 5 cut(s) 27, 193, 215, 225, 242
Lsp1109I GCAGC 1 cut(s) 245
MaeI CTAG 1 cut(s) 155
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 1 cut(s) 230
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 229
MmeI TCCRAC 1 cut(s) 143
MnlI CCTC 1 cut(s) 159
MseI TTAA 2 cut(s) 84, 144
MspA1I CMGCKG 1 cut(s) 215
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 1 cut(s) 22
NheI GCTAGC 1 cut(s) 154
NlaIII CATG 2 cut(s) 162, 185
NlaIV GGNNCC 2 cut(s) 102, 226
NspI RCATGY 2 cut(s) 162, 185
PciI ACATGT 1 cut(s) 181
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 1 cut(s) 235
PscI ACATGT 1 cut(s) 181
PshBI ATTAAT 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 102, 226
PstI CTGCAG 1 cut(s) 81
PsuI RGATCY 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 84, 144
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 1 cut(s) 22
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 1 cut(s) 229
SetI ASST 6 cut(s) 8, 106, 156, 205, 217, 238
SfcI CTRYAG 1 cut(s) 77
SspI AATATT 1 cut(s) 175
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 1 cut(s) 104
TaaI ACNGT 1 cut(s) 196
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 2 cut(s) 84, 144
Tru9I TTAA 2 cut(s) 84, 144
TscAI CASTG 2 cut(s) 81, 199
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 51, 108
TspRI CASTG 2 cut(s) 81, 199
VspI ATTAAT 1 cut(s) 84
XceI RCATGY 2 cut(s) 162, 185
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.