Rorug05G0483100

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002507
Physical Location & Seq
Forward (+)
66441307 .. 66446101
4795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0483100.1

Sequence Viewer

Length: 1176 bp
ATGGTGACCGTCGACGAAGTCCGCAAGGCTCAACGGGCTGAGGGTCCGGCCACCATCTTGGGCATCGGGACAGCAACTCCTCCCAATTGTATCGACCAGAGCACATACCCCGACTACTACTTTCGTATAACCAATAGTGAGCACAAGGCTGATCTCAAGGAGAAATTCCAGCGCATGTGTGACAAGTCAATGATCAAGAAGCGTTACATGTATTTGACTGAAGAGATCTTGAAAGAGAATCCTAGTATGTGCGAGTACATGGCACCTTCACTTGATGCAAGACAAGATATGGTGGTTGCTGAAATTCCAGAGTTGGGCAAAGAGGCTGCCACCAAGGCCATTAAGGAATGGGGCCAGCCCAAGTCCAAAATCACCCACTTGGTCTTTTGTACCAGCAGTGGTGTTGACATGCCCGGGGCCGATTACCAGCTCACTAAGCTTTTGGGCCTCAGCCCGTCTGTCAAGCGCTTCATGATGTACCAACAAGGTTGTTCCGCCGGCGGCACTGTGCTCCGGTTGGCCAAGGACTTGGCCGAGAACAATCGGGATGCACGTGTTCTCGTTGTTTGCTCTGAGATCACGGCCATCTTTTTTCGTGGGCCTAGCGACACCCATCTTGATAGTCTTGTGGGCCAAGCCTTGTTCAGTGACGGTGCTGCCGCTGTTATTGTTGGGGCCGACCCGTTGCCCGAGATCGAGAAGCCCTTGTTTGAGGTTGTCTCGGCGGCCCAGACTATCCTTCCCGATAGTGAAGGGGCCATTGAATTGCATCTTCGTGAAGCTGGGCTCACATTTCACCTCCTCAAGGATGTTCCCGAGCTGATTTCGAAGAACATTGAGAAGAGCCTCAACGAGGCCTTCAAGCCTTTGAACATCACAGATTGGAACTCACTTTTTTGGATTGTACACCCAGGTGGCCCTGCAATTCTGGACCAAGTAGAGGCAAAATTAGGCCTGAAGGCCGAAAAGTTAGAAGCCACAAGGCACATATTATCCGAGTACGGCAACATGATTAGTCCTTGTGTGTTGTTTATTTTGGATGAGGTGAGGAAGAAGTCTGCAGAAAATGGGCACAAGACTACTGGAGAGGGCTTGGAGTGGGGAGTCCTATTTGGGTTTGGGCCTGGGCTCACTGTCGAGACCGTCGTGCTTCACAGTGTTGGTGTCACAGCTTGA

Protein Analysis

391

Amino Acids

42.91

Weight (kDa)

5.42

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chal_sti_synt_N PF00195 5 - 228 8.6e-120 Chalcone and stilbene synthases, N-terminal domain
FAE1_CUT1_RppA PF08392 123 - 227 2.7e-06 FAE1/Type III polyketide synthase-like protein
Chal_sti_synt_C PF02797 238 - 387 1.2e-68 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 262
AccI GTMKAC 1 cut(s) 12
AciI CCGC 5 cut(s) 22, 495, 501, 660, 725
AcoI YGGCCR 4 cut(s) 48, 519, 531, 582
AcsI RAATTY 2 cut(s) 164, 303
AcuI CTGAAG 2 cut(s) 240, 977
AcvI CACGTG 1 cut(s) 554
AfaI GTAC 5 cut(s) 257, 391, 479, 906, 1001
AfeI AGCGCT 1 cut(s) 467
AfiI CCNNNNNNNGG 4 cut(s) 314, 805, 853, 940
AflIII ACRYGT 2 cut(s) 207, 553
AgsI TTSAA 4 cut(s) 232, 764, 862, 871
AjnI CCWGG 2 cut(s) 910, 1123
AleI CACNNNNGTG 1 cut(s) 912
AluBI AGCT 5 cut(s) 430, 439, 782, 820, 1172
AluI AGCT 5 cut(s) 430, 439, 782, 820, 1172
Alw21I GWGCWC 3 cut(s) 104, 144, 513
Alw26I GTCTC 2 cut(s) 724, 1133
Ama87I CYCGRG 3 cut(s) 413, 689, 815
Aor51HI AGCGCT 1 cut(s) 467
ApeKI GCWGC 2 cut(s) 326, 656
ApoI RAATTY 2 cut(s) 164, 303
AspLEI GCGC 2 cut(s) 174, 468
AsuC2I CCSGG 2 cut(s) 414, 415
AsuHPI GGTGA 4 cut(s) 16, 364, 788, 1057
AsuII TTCGAA 1 cut(s) 827
AvaI CYCGRG 3 cut(s) 413, 689, 815
AvaII GGWCC 2 cut(s) 44, 931
BaeGI GKGCMC 1 cut(s) 1074
BalI TGGCCA 1 cut(s) 521
BanI GGYRCC 1 cut(s) 262
BanII GRGCYC 2 cut(s) 789, 1131
BbrPI CACGTG 1 cut(s) 554
Bbv12I GWGCWC 3 cut(s) 104, 144, 513
BbvCI CCTCAGC 2 cut(s) 39, 449
BbvI GCAGC 2 cut(s) 313, 643
BccI CCATC 3 cut(s) 62, 593, 621
BceAI ACGGC 2 cut(s) 597, 1018
BciT130I CCWGG 2 cut(s) 912, 1125
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 2 cut(s) 414, 415
BcoDI GTCTC 2 cut(s) 724, 1133
BfaI CTAG 2 cut(s) 243, 603
BfmI CTRYAG 1 cut(s) 1059
BfoI RGCGCY 1 cut(s) 469
BglI GCCNNNNNGGC 1 cut(s) 335
BglII AGATCT 1 cut(s) 225
BisI GCNGC 5 cut(s) 327, 502, 657, 660, 726
BlsI GCNGC 5 cut(s) 328, 503, 658, 661, 727
Bme1390I CCNGG 4 cut(s) 414, 415, 912, 1125
Bme18I GGWCC 2 cut(s) 44, 931
BmeT110I CYCGRG 3 cut(s) 413, 689, 815
BmiI GGNNCC 6 cut(s) 45, 264, 353, 418, 676, 757
BmrFI CCNGG 4 cut(s) 414, 415, 912, 1125
BmsI GCATC 4 cut(s) 72, 265, 538, 778
BplI GAGNNNNNCTC 2 cut(s) 704, 736
BpmI CTGGAG 1 cut(s) 1104
Bpu10I CCTNAGC 2 cut(s) 39, 449
Bpu14I TTCGAA 1 cut(s) 827
BpuEI CTTGAG 2 cut(s) 140, 788
BpuMI CCSGG 2 cut(s) 414, 415
BsaAI YACGTR 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 1133
BsaJI CCNNGG 6 cut(s) 333, 413, 414, 522, 910, 1124
BsaWI WCCGGW 1 cut(s) 513
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 805, 853, 940
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 1087
BseBI CCWGG 2 cut(s) 912, 1125
BseDI CCNNGG 6 cut(s) 333, 413, 414, 522, 910, 1124
BseGI GGATG 3 cut(s) 553, 814, 1045
BseLI CCNNNNNNNGG 4 cut(s) 314, 805, 853, 940
BseMII CTCAG 3 cut(s) 30, 463, 564
BseNI ACTGG 1 cut(s) 1087
BseRI GAGGAG 2 cut(s) 69, 791
BseSI GKGCMC 1 cut(s) 1074
BseXI GCAGC 2 cut(s) 313, 643
BseYI CCCAGC 1 cut(s) 782
BshNI GGYRCC 1 cut(s) 262
BsiHKAI GWGCWC 3 cut(s) 104, 144, 513
BsiHKCI CYCGRG 3 cut(s) 413, 689, 815
BsiSI CCGG 4 cut(s) 47, 414, 498, 514
BslFI GGGAC 1 cut(s) 82
BslI CCNNNNNNNGG 4 cut(s) 314, 805, 853, 940
BsmAI GTCTC 2 cut(s) 724, 1133
BsmFI GGGAC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 1133
BsoBI CYCGRG 3 cut(s) 413, 689, 815
Bsp119I TTCGAA 1 cut(s) 827
Bsp1286I GDGCHC 6 cut(s) 104, 144, 513, 789, 1074, 1131
Bsp1407I TGTACA 1 cut(s) 904
Bsp143I GATC 5 cut(s) 151, 192, 225, 576, 693
BspACI CCGC 5 cut(s) 22, 495, 501, 660, 725
BspCNI CTCAG 3 cut(s) 31, 462, 565
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 6 cut(s) 45, 264, 353, 418, 676, 757
BspMAI CTGCAG 1 cut(s) 1063
BspQI GCTCTTC 1 cut(s) 836
BspT104I TTCGAA 1 cut(s) 827
BspT107I GGYRCC 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 1133
BsrFI RCCGGY 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 904
BsrI ACTGG 1 cut(s) 1087
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 6 cut(s) 333, 413, 414, 522, 910, 1124
BssMI GATC 5 cut(s) 151, 192, 225, 576, 693
BssT1I CCWWGG 2 cut(s) 333, 522
Bst2UI CCWGG 2 cut(s) 912, 1125
Bst4CI ACNGT 6 cut(s) 10, 508, 653, 1135, 1144, 1157
Bst6I CTCTTC 2 cut(s) 216, 836
BstAUI TGTACA 1 cut(s) 904
BstBAI YACGTR 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 2 cut(s) 356, 499
BstDEI CTNAG 4 cut(s) 39, 435, 449, 573
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 2 cut(s) 803, 851
BstF5I GGATG 3 cut(s) 553, 814, 1045
BstH2I RGCGCY 1 cut(s) 469
BstHHI GCGC 2 cut(s) 174, 468
BstKTI GATC 5 cut(s) 154, 195, 228, 579, 696
BstMAI GTCTC 2 cut(s) 724, 1133
BstMBI GATC 5 cut(s) 151, 192, 225, 576, 693
BstMWI GCNNNNNNNGC 3 cut(s) 35, 335, 436
BstNI CCWGG 2 cut(s) 912, 1125
BstNSI RCATGY 3 cut(s) 178, 211, 412
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 4 cut(s) 412, 413, 910, 1123
BstSFI CTRYAG 1 cut(s) 1059
BstSLI GKGCMC 1 cut(s) 1074
BstV1I GCAGC 2 cut(s) 313, 643
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 2 cut(s) 58, 529
BstYI RGATCY 1 cut(s) 225
BtsCI GGATG 3 cut(s) 553, 814, 1045
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 5 cut(s) 403, 504, 652, 1131, 1162
Cac8I GCNNGC 2 cut(s) 356, 499
CciI TCATGA 1 cut(s) 471
CfoI GCGC 2 cut(s) 174, 468
Cfr10I RCCGGY 1 cut(s) 497
Cfr9I CCCGGG 1 cut(s) 413
Csp6I GTAC 5 cut(s) 256, 390, 478, 905, 1000
CviAII CATG 6 cut(s) 175, 208, 259, 409, 472, 1009
CviQI GTAC 5 cut(s) 256, 390, 478, 905, 1000
DdeI CTNAG 4 cut(s) 39, 435, 449, 573
DpnI GATC 5 cut(s) 153, 194, 227, 578, 695
DpnII GATC 5 cut(s) 151, 192, 225, 576, 693
EaeI YGGCCR 4 cut(s) 48, 519, 531, 582
Eam1104I CTCTTC 2 cut(s) 216, 836
EarI CTCTTC 2 cut(s) 216, 836
EciI GGCGGA 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 333, 522
Eco147I AGGCCT 2 cut(s) 857, 954
Eco24I GRGCYC 2 cut(s) 789, 1131
Eco31I GGTCTC 1 cut(s) 1133
Eco47I GGWCC 2 cut(s) 44, 931
Eco47III AGCGCT 1 cut(s) 467
Eco57I CTGAAG 2 cut(s) 240, 977
Eco72I CACGTG 1 cut(s) 554
Eco88I CYCGRG 3 cut(s) 413, 689, 815
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 2 cut(s) 803, 851
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 910, 1123
EcoT14I CCWWGG 2 cut(s) 333, 522
EcoT38I GRGCYC 2 cut(s) 789, 1131
ErhI CCWWGG 2 cut(s) 333, 522
FaeI CATG 6 cut(s) 178, 211, 262, 412, 475, 1012
FaqI GGGAC 1 cut(s) 82
FatI CATG 6 cut(s) 174, 207, 258, 408, 471, 1008
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 5 cut(s) 327, 502, 657, 660, 726
FokI GGATG 3 cut(s) 560, 821, 1052
FriOI GRGCYC 2 cut(s) 789, 1131
Fsp4HI GCNGC 5 cut(s) 327, 502, 657, 660, 726
FspBI CTAG 2 cut(s) 243, 603
GlaI GCGC 2 cut(s) 173, 467
GluI GCNGC 5 cut(s) 327, 502, 657, 660, 726
GsaI CCCAGC 1 cut(s) 786
GsuI CTGGAG 1 cut(s) 1104
HaeII RGCGCY 1 cut(s) 469
HapII CCGG 4 cut(s) 47, 414, 498, 514
HhaI GCGC 2 cut(s) 174, 468
Hin1II CATG 6 cut(s) 178, 211, 262, 412, 475, 1012
Hin6I GCGC 2 cut(s) 172, 466
HinP1I GCGC 2 cut(s) 172, 466
HincII GTYRAC 2 cut(s) 13, 406
HindII GTYRAC 2 cut(s) 13, 406
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 2 cut(s) 238, 1104
HpaII CCGG 4 cut(s) 47, 414, 498, 514
HphI GGTGA 4 cut(s) 16, 364, 788, 1057
Hpy166II GTNNAC 3 cut(s) 13, 406, 907
Hpy188I TCNGA 2 cut(s) 574, 997
Hpy8I GTNNAC 3 cut(s) 13, 406, 907
Hpy99I CGWCG 3 cut(s) 14, 17, 1148
HpyAV CCTTC 5 cut(s) 276, 746, 749, 868, 952
HpyCH4III ACNGT 6 cut(s) 10, 508, 653, 1135, 1144, 1157
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 5 cut(s) 278, 551, 769, 923, 1061
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 335, 436
HpyF3I CTNAG 4 cut(s) 39, 435, 449, 573
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 6 cut(s) 178, 211, 262, 412, 475, 1012
HspAI GCGC 2 cut(s) 172, 466
KroI GCCGGC 1 cut(s) 497
KroNI GCCGGC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 5 cut(s) 151, 192, 225, 576, 693
LguI GCTCTTC 1 cut(s) 836
LmnI GCTCC 1 cut(s) 516
Lsp1109I GCAGC 2 cut(s) 313, 643
LweI GCATC 4 cut(s) 72, 265, 538, 778
MaeI CTAG 2 cut(s) 243, 603
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 5 cut(s) 4, 179, 203, 647, 1165
MalI GATC 5 cut(s) 153, 194, 227, 578, 695
MboI GATC 5 cut(s) 151, 192, 225, 576, 693
MboII GAAGA 5 cut(s) 233, 764, 841, 853, 1063
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 6 cut(s) 104, 144, 513, 789, 1074, 1131
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 6 cut(s) 85, 164, 303, 764, 924, 947
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 1 cut(s) 1113
Mox20I TGGCCA 1 cut(s) 521
MreI CGCCGGCG 1 cut(s) 497
MroNI GCCGGC 1 cut(s) 497
MscI TGGCCA 1 cut(s) 521
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 2 cut(s) 774, 912
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 4 cut(s) 47, 414, 498, 514
MspR9I CCNGG 4 cut(s) 414, 415, 912, 1125
MunI CAATTG 1 cut(s) 85
MvaI CCWGG 2 cut(s) 912, 1125
MwoI GCNNNNNNNGC 3 cut(s) 35, 335, 436
NaeI GCCGGC 1 cut(s) 499
NciI CCSGG 2 cut(s) 414, 415
NdeII GATC 5 cut(s) 151, 192, 225, 576, 693
NgoMIV GCCGGC 1 cut(s) 497
NlaIII CATG 6 cut(s) 178, 211, 262, 412, 475, 1012
NlaIV GGNNCC 6 cut(s) 45, 264, 353, 418, 676, 757
NmeAIII GCCGAG 2 cut(s) 559, 701
NmuCI GTSAC 4 cut(s) 4, 179, 647, 1165
NspI RCATGY 3 cut(s) 178, 211, 412
NspV TTCGAA 1 cut(s) 827
OliI CACNNNNGTG 1 cut(s) 912
PagI TCATGA 1 cut(s) 471
PceI AGGCCT 2 cut(s) 857, 954
PciI ACATGT 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 836
PcsI WCGNNNNNNNCGW 2 cut(s) 550, 1143
PdiI GCCGGC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 238
PflFI GACNNNGTC 1 cut(s) 17
PkrI GCNGC 5 cut(s) 328, 503, 658, 661, 727
PleI GAGTC 1 cut(s) 1112
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
PpsI GAGTC 1 cut(s) 1112
Ppu21I YACGTR 1 cut(s) 554
PscI ACATGT 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 910, 1123
PspCI CACGTG 1 cut(s) 554
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 1 cut(s) 782
PspGI CCWGG 2 cut(s) 910, 1123
PspN4I GGNNCC 6 cut(s) 45, 264, 353, 418, 676, 757
PstI CTGCAG 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 17
RsaI GTAC 5 cut(s) 257, 391, 479, 906, 1001
RsaNI GTAC 5 cut(s) 256, 390, 478, 905, 1000
RseI CAYNNNNRTG 2 cut(s) 774, 912
SalI GTCGAC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 836
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 5 cut(s) 327, 502, 657, 660, 726
Sau3AI GATC 5 cut(s) 151, 192, 225, 576, 693
SchI GAGTC 1 cut(s) 1113
ScrFI CCNGG 4 cut(s) 414, 415, 912, 1125
SduI GDGCHC 6 cut(s) 104, 144, 513, 789, 1074, 1131
SfaNI GCATC 4 cut(s) 72, 265, 538, 778
SfcI CTRYAG 1 cut(s) 1059
SfuI TTCGAA 1 cut(s) 827
SgrAI CRCCGGYG 1 cut(s) 497
SgrDI CGTCGACG 1 cut(s) 11
SinI GGWCC 2 cut(s) 44, 931
SmaI CCCGGG 1 cut(s) 415
SmiMI CAYNNNNRTG 2 cut(s) 774, 912
SmlI CTYRAG 2 cut(s) 155, 803
SmoI CTYRAG 2 cut(s) 155, 803
Sse9I AATT 6 cut(s) 85, 164, 303, 764, 924, 947
SseBI AGGCCT 2 cut(s) 857, 954
SsiI CCGC 5 cut(s) 22, 495, 501, 660, 725
SspMI CTAG 2 cut(s) 243, 603
StuI AGGCCT 2 cut(s) 857, 954
StyD4I CCNGG 4 cut(s) 412, 413, 910, 1123
StyI CCWWGG 2 cut(s) 333, 522
TaaI ACNGT 6 cut(s) 10, 508, 653, 1135, 1144, 1157
TaiI ACGT 1 cut(s) 556
TaqI TCGA 5 cut(s) 12, 93, 696, 827, 1137
TasI AATT 6 cut(s) 85, 164, 303, 764, 924, 947
TatI WGTACW 2 cut(s) 255, 904
TauI GCSGC 3 cut(s) 504, 662, 728
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 5 cut(s) 403, 511, 652, 1138, 1162
TseFI GTSAC 4 cut(s) 4, 179, 647, 1165
TseI GCWGC 2 cut(s) 326, 656
Tsp45I GTSAC 4 cut(s) 4, 179, 647, 1165
TspDTI ATGAA 1 cut(s) 460
TspMI CCCGGG 1 cut(s) 413
TspRI CASTG 5 cut(s) 403, 511, 652, 1138, 1162
Tth111I GACNNNGTC 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 44, 931
XagI CCTNNNNNAGG 2 cut(s) 803, 851
XapI RAATTY 2 cut(s) 164, 303
XceI RCATGY 3 cut(s) 178, 211, 412
XmaI CCCGGG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 2 cut(s) 243, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.