RchiOBHm_Chr3g0449031

Dehydration-responsive element-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003740
Physical Location & Seq
Reverse (-)
992494 .. 992994
501 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41643

Sequence Viewer

Length: 501 bp
ATGGACAATGACCAAGAGAGAAAGGTGACGAGGAAAGCCAACATGGGAAGGTCGAGAAAGGGCTCCATGAAAGGGAAGGGAGGCCCTGAGAACGCTACGTGCACTTTCAGAGGAGTCAGGCAAAGAACTTGGGGCAAATGGGTGGCGGAAATACGTGAGCCCAAACGCGGTGCTAGGCTTTGGCTAGGCACTTTCAACACTTCCACGGAGGCAGCGCTGGCTTATGACGATGCGGCTCGAAAGCTGTACGGCTCCTCCGCCAAGCTCAACTTGCCGGACCACTCCTCCTCCTCCTCATCCTCCTCCTCCACATCACCGTCTTCGAAAACGAGTTCTGCTGATAAAGGTTGTGGTGAACACAAGCATCACTACTACAAAAAGGGCCTAAGAGGACTAATAATATGTGTGCCTTTTGATACCTATAGGGACAAATATGTATCCCCATTGAGCTATAGAGACATATATCATATGTGTGCCTATTGTTTGTGTTCCTATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

166

Amino Acids

18.57

Weight (kDa)

9.62

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 36 - 84 4.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 168
AciI CCGC 4 cut(s) 146, 168, 233, 258
AfaI GTAC 1 cut(s) 248
AfeI AGCGCT 1 cut(s) 216
AfiI CCNNNNNNNGG 2 cut(s) 72, 167
AgsI TTSAA 1 cut(s) 196
AluBI AGCT 3 cut(s) 244, 265, 450
AluI AGCT 3 cut(s) 244, 265, 450
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 450
Alw44I GTGCAC 1 cut(s) 100
Aor51HI AGCGCT 1 cut(s) 216
AoxI GGCC 2 cut(s) 82, 382
ApaLI GTGCAC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 3 cut(s) 83, 277, 382
AsuHPI GGTGA 3 cut(s) 37, 306, 365
AsuII TTCGAA 1 cut(s) 323
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 65, 162
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 265
BciVI GTATCC 1 cut(s) 448
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 2 cut(s) 174, 185
BfmI CTRYAG 2 cut(s) 421, 451
BfoI RGCGCY 1 cut(s) 218
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 2 cut(s) 213, 234
BlsI GCNGC 2 cut(s) 214, 235
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 3 cut(s) 83, 277, 382
BmiI GGNNCC 2 cut(s) 64, 253
BmsI GCATC 2 cut(s) 220, 373
BpiI GAAGAC 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 323
BsaAI YACGTR 2 cut(s) 99, 155
BsaJI CCNNGG 1 cut(s) 204
BsaXI ACNNNNNCTCC 6 cut(s) 239, 269, 269, 272, 299, 302
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 167
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 296
BseLI CCNNNNNNNGG 2 cut(s) 72, 167
BseMII CTCAG 1 cut(s) 78
BseRI GAGGAG 8 cut(s) 126, 244, 274, 277, 280, 283, 292, 295
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 84, 384
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 72, 167
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 2 cut(s) 84, 384
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 3 cut(s) 65, 104, 162
BspACI CCGC 4 cut(s) 146, 168, 233, 258
BspANI GGCC 2 cut(s) 84, 384
BspCNI CTCAG 1 cut(s) 79
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 2 cut(s) 64, 253
BspT104I TTCGAA 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 318
BstBAI YACGTR 2 cut(s) 99, 155
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 2 cut(s) 87, 386
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 296
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 450
BstMWI GCNNNNNNNGC 2 cut(s) 218, 271
BstSFI CTRYAG 2 cut(s) 421, 451
BstSLI GKGCMC 1 cut(s) 104
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 224
BstV2I GAAGAC 1 cut(s) 312
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 2 cut(s) 84, 384
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 1 cut(s) 296
Cac8I GCNNGC 1 cut(s) 219
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 83, 277, 382
Csp6I GTAC 1 cut(s) 247
CviAII CATG 2 cut(s) 43, 67
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 2 cut(s) 87, 386
EciI GGCGGA 2 cut(s) 161, 247
Eco24I GRGCYC 2 cut(s) 65, 162
Eco47I GGWCC 1 cut(s) 277
Eco47III AGCGCT 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 83, 382
EcoT38I GRGCYC 2 cut(s) 65, 162
FaeI CATG 2 cut(s) 46, 70
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 440
FatI CATG 2 cut(s) 42, 66
FauNDI CATATG 1 cut(s) 468
Fnu4HI GCNGC 2 cut(s) 213, 234
FokI GGATG 1 cut(s) 283
FriOI GRGCYC 2 cut(s) 65, 162
Fsp4HI GCNGC 2 cut(s) 213, 234
FspBI CTAG 2 cut(s) 174, 185
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 213, 234
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 2 cut(s) 84, 384
HapII CCGG 1 cut(s) 275
HhaI GCGC 1 cut(s) 217
Hin1II CATG 2 cut(s) 46, 70
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 1 cut(s) 114
HpaII CCGG 1 cut(s) 275
HphI GGTGA 3 cut(s) 37, 306, 365
Hpy166II GTNNAC 2 cut(s) 102, 356
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 1 cut(s) 54
Hpy8I GTNNAC 2 cut(s) 102, 356
HpyAV CCTTC 2 cut(s) 42, 70
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 2 cut(s) 98, 154
HpyCH4V TGCA 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 271
HpyF3I CTNAG 2 cut(s) 87, 386
HpySE526I ACGT 2 cut(s) 98, 154
Hsp92II CATG 2 cut(s) 46, 70
HspAI GCGC 1 cut(s) 215
LmnI GCTCC 2 cut(s) 68, 257
LpnPI CCDG 4 cut(s) 99, 103, 203, 288
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 2 cut(s) 220, 373
MaeI CTAG 2 cut(s) 174, 185
MaeII ACGT 2 cut(s) 98, 154
MaeIII GTNAC 1 cut(s) 25
MboII GAAGA 1 cut(s) 312
MhlI GDGCHC 3 cut(s) 65, 104, 162
MlyI GAGTC 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 471
MspI CCGG 1 cut(s) 275
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 218, 271
NdeI CATATG 1 cut(s) 468
NlaIII CATG 2 cut(s) 46, 70
NlaIV GGNNCC 2 cut(s) 64, 253
NmuCI GTSAC 1 cut(s) 25
NspV TTCGAA 1 cut(s) 323
PkrI GCNGC 2 cut(s) 214, 235
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
Ppu21I YACGTR 2 cut(s) 99, 155
PspN4I GGNNCC 2 cut(s) 64, 253
PspPI GGNCC 3 cut(s) 83, 277, 382
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 471
SatI GCNGC 2 cut(s) 213, 234
Sau96I GGNCC 3 cut(s) 83, 277, 382
SchI GAGTC 1 cut(s) 123
SduI GDGCHC 3 cut(s) 65, 104, 162
SetI ASST 9 cut(s) 27, 53, 101, 157, 246, 267, 349, 422, 452
SfaNI GCATC 2 cut(s) 220, 373
SfcI CTRYAG 2 cut(s) 421, 451
SfuI TTCGAA 1 cut(s) 323
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 471
SsiI CCGC 4 cut(s) 146, 168, 233, 258
SspMI CTAG 2 cut(s) 174, 185
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 2 cut(s) 101, 157
TaqI TCGA 3 cut(s) 53, 238, 323
TauI GCSGC 1 cut(s) 236
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 1 cut(s) 221
VneI GTGCAC 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 277
XspI CTAG 2 cut(s) 174, 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.