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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 167 |
| AfaI | GTAC | 1 cut(s) | 126 |
| AgsI | TTSAA | 1 cut(s) | 51 |
| AoxI | GGCC | 1 cut(s) | 167 |
| AsuHPI | GGTGA | 2 cut(s) | 87, 146 |
| BbsI | GAAGAC | 1 cut(s) | 185 |
| BccI | CCATC | 2 cut(s) | 115, 228 |
| BciVI | GTATCC | 1 cut(s) | 23 |
| BfaI | CTAG | 2 cut(s) | 20, 104 |
| BfuI | GTATCC | 1 cut(s) | 23 |
| BpiI | GAAGAC | 1 cut(s) | 185 |
| BsaJI | CCNNGG | 1 cut(s) | 145 |
| BseDI | CCNNGG | 1 cut(s) | 145 |
| BseGI | GGATG | 1 cut(s) | 126 |
| BsgI | GTGCAG | 1 cut(s) | 83 |
| BshFI | GGCC | 1 cut(s) | 169 |
| BsnI | GGCC | 1 cut(s) | 169 |
| BspANI | GGCC | 1 cut(s) | 169 |
| BssECI | CCNNGG | 1 cut(s) | 145 |
| Bst4CI | ACNGT | 1 cut(s) | 129 |
| BstF5I | GGATG | 1 cut(s) | 126 |
| BstV2I | GAAGAC | 1 cut(s) | 185 |
| BsuI | GTATCC | 1 cut(s) | 23 |
| BsuRI | GGCC | 1 cut(s) | 169 |
| BtsCI | GGATG | 1 cut(s) | 126 |
| Csp6I | GTAC | 1 cut(s) | 125 |
| CviJI | RGCY | 1 cut(s) | 169 |
| CviKI_1 | RGCY | 1 cut(s) | 169 |
| CviQI | GTAC | 1 cut(s) | 125 |
| EaeI | YGGCCR | 1 cut(s) | 167 |
| FaiI | YATR | 1 cut(s) | 7 |
| FokI | GGATG | 1 cut(s) | 133 |
| FspBI | CTAG | 2 cut(s) | 20, 104 |
| HaeIII | GGCC | 1 cut(s) | 169 |
| HphI | GGTGA | 2 cut(s) | 87, 146 |
| Hpy188I | TCNGA | 1 cut(s) | 146 |
| Hpy188III | TCNNGA | 1 cut(s) | 197 |
| HpyAV | CCTTC | 1 cut(s) | 6 |
| HpyCH4III | ACNGT | 1 cut(s) | 129 |
| HpyCH4IV | ACGT | 1 cut(s) | 151 |
| HpyCH4V | TGCA | 1 cut(s) | 64 |
| HpySE526I | ACGT | 1 cut(s) | 151 |
| MaeI | CTAG | 2 cut(s) | 20, 104 |
| MaeII | ACGT | 1 cut(s) | 151 |
| MboII | GAAGA | 2 cut(s) | 185, 204 |
| MnlI | CCTC | 4 cut(s) | 64, 100, 140, 185 |
| MseI | TTAA | 1 cut(s) | 180 |
| RsaI | GTAC | 1 cut(s) | 126 |
| RsaNI | GTAC | 1 cut(s) | 125 |
| SaqAI | TTAA | 1 cut(s) | 180 |
| SetI | ASST | 5 cut(s) | 100, 111, 136, 154, 177 |
| SgeI | CNNG | 8 cut(s) | 32, 36, 116, 158, 170, 198, 209, 214 |
| SspMI | CTAG | 2 cut(s) | 20, 104 |
| TaaI | ACNGT | 1 cut(s) | 129 |
| TaiI | ACGT | 1 cut(s) | 154 |
| Tru1I | TTAA | 1 cut(s) | 180 |
| Tru9I | TTAA | 1 cut(s) | 180 |
| TspGWI | ACGGA | 1 cut(s) | 100 |
| XspI | CTAG | 2 cut(s) | 20, 104 |