Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001557

Physical Location & Seq

Genomic Coordinates Forward (+)

52594719 .. 52600157

Gene Structure 5439 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003777

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGGCCAATCCTACTTACCAGGAATCCTTGTCATTTGGAGACCCAGACCATCCTCAGGGTCAAGAAGAAGTTATTCCATATGAGAGTGGAACCCTGAAGGTTTTGCTACATGGGTCCCTGGTCATATGGGTCAAGGAGGCCAAAAACCTTCCTGACATGGAGAGTGTTCGCAAGCGTGTGGGGCATGTAATTGCGAAGCTGCCTGGGACGAGAGACAAAAATATAATCAGTTCTCCATATGTTACGATTTCTTTATCAAATGCTGTAATTGCGAGGACCTTTGTGAAGAGTACTATCGAGAACCCTGTGTGGGAGCAACATTTTGATGTTCCTGTTGCTCATTATGCTTCACAAGTGAACTTTGTTGTGAAACACAGTGATGTTTTCGGCTCTAAGATCATAGGTTTTGTTGGAATCTCAGCTCAGCAGTTGTACTCTGGCATCAAAGTTGAGGGAACCTTCCCTATCCTTGACACCACCAGTGGTAAAAGCTGTATGCCTGGAGTTGTGCTGAGCTTGTCAATTCAGTATACCGCGATTGAAAAAATGGCATTTTATAATCGCGGAGTAGGCTTAGATCCTGATCATCAAGGGGTCCCTGGTACTTACTTTCCTGCTAGGAAAGGCGGTAAAGTTACTCTTTATCAAGATGCTCATGCCCCTGATGGTTGCCTCCCCAACTTCAAGCTTGATGGTGGTCTTAAGTACGAGCATGGAAATTGTTGGCAAGACATATTTGATGCAATAAGTCAGGCCTGCCATTTGATATACATAACTGGGTGGTCACTGAACCCCAAAGTCAGATTGCTTCGGAATAATAATGGTTCATCAGATAGCACATTGGGTGATCTGCTGAAGACCAAGTCACAGAAAGGTGTGAGAGTTCTGCTTCTTGTTTGGGACGATCCAACTTCTATTAAGGGATTTAAAATGGAAGGGCTCATGCATACCAATGATGAAGACGCTCGACGTTTCTTTAAGGACTCTTCAGTTCAATTTCTACTCTGCCCTCGATCATCAGTCCAAAGCTGGGGGAAAAAGATGGAAGCTGGAATAGTCTATACCCATCATCAGAAAACTGTAATTGTGGACGCTGATGCTGGTCATTCTAAAAGAAAGATCATTGCATTTGTTGGAGGTCTTGACTTGTGCTTGGGACGTTATGATACTCCGGAGCATTCTATATTTCGGACCTTACGGACAGTGCACAAGGATGACAATTATAACCCTACTTTCTCGAAACCCGTTATTGGGTGTCCAAGGCAGCCATGGCATGATTTGCACAGTAAAATTGATGGTCCAGCAGCTTATGACATCCTCACCAATTTTGAGCAGCGTTGGGGGATGGCTTCAAAACCACATGGTCTTCACAAACTAAAAGAACGAGGACTTCAAAAACTGAAATTACGAGGAGATGATGATGATGCATTACTCAAGATTGAGAAAATTCCTGAAATTCTTGGGATGACAGAGGTTCCTCGCTTGAGCGAGGATGATAAAGAAGCTTGGGATATTCAGGTTTTCCGTTCAATTGATTCCCATTCAGTGAAAGGATTTCCCGAAGACCCTGGAGAAGCTACAAGGATGAACCTTGTATGTGGGAAAAATGTGCTGATAGACATGAGTATACATGCAGCATATGTAAGAGCAATCCGTGCGGCTCAACATTTTATTTACATAGAGAACCAGTACTTTCTTGGATCATCATATAATTGGAATTCAGACATTAGTGATACAGGTGCAAACAATTTGATACCGATGGAAATCGCTCTGAAAATAGCAAACAAAATCAAAGCAAATGAGAAGTTTTCTGTATATATTGTCATCCCCATGTGGCCAGAAGGTGATCCCAATAGTATGCCTCTTCAAAGAATTCTCCTTTGGCAGCATAAAACAATGCAAATGATGTATGAAATCATTTACAAGGCCCTAAAGGAGGTTGGACTCGAAAATAAGTACTGTCCACAAGACTATTTGAATTTTTTTTGCCTTGGAAATCGTGAGAAACCAAGTGGAGAAGGCAATTCTGCTGCAAATAATTCAAGTGCAGCAAACTCTCCTGAAGTACTCACTCAGAATAGTCGGCGATTTATGATTTATGTCCATTCTAAAGGAATGATAGTGGATGATGAATATGTTATAGTAGGATCTGCAAATATCAATCAGCGATCCATGGAAGGTCTCCGAGATACTGAAATAGCAATGGGGGCTTATCAGCCTCATCATACTTGGGCGCGAAAACTCTCAAGTCCACACGGAAAGATATATGGATACAGAATGTCACTGTGGGCAGAGCATTTAGGACTTGTTGAAGAATGTTTCAATCAGCCAGAGAGTCTTGAATGTGTTCAAAGGGTAAGGTCTTTGGGTGAGCAGAACTGGAGTCAATACTTGGCTGATGAAGTCACAGAAATGAAGGGTCACCTCCTCAAGTATCCGGTTGAAGTTAGTCCAACGGGCACAGTGGGGCCTCTTCCAGGATGTCCAACATTTCCAGACGTTGGTGGCAATATATTGGGGAGTCTCGGCGATGTAAAAATGAAGAATCTGCGTATCAGTAGCACTGGGAGGACAATATCACTTAGAGGCGCCATCATCACCACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0005911 GO:0006629 GO:0006643 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008289 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009816 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010154 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0022414 GO:0022626 GO:0030054 GO:0032501 GO:0032502 GO:0032991 GO:0035091 GO:0042221 GO:0042578 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045087 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0061458 GO:0071704 GO:0071944 GO:0098542 GO:1901981 GO:1902936 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

869

Amino Acids

97.04

Weight (kDa)

7.18

Isoelectric Point (pI)

38.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	38 - 155	1.7e-12	C2 domain
PLDc_2	PF13091	684 - 735	2.1e-06	PLD-like domain
PLDc	PF00614	699 - 724	2.8e-08	Phospholipase D Active site motif
PLD_C	PF12357	771 - 841	7.7e-30	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11830 AT4G11830 AT4G11830 AT4G11830 AT4G11840 AT4G11840 AT4G11850
fragaria_vesca	FvH4_5g01274 FvH4_5g01290 FvH4_5g01290 FvH4_5g01290
malus_domestica	MD04G1069600.v1.1
prunus_persica	Prupe.5G088300_v2.0.a1 Prupe.5G088300_v2.0.a1
pyrus_communis	pycom04g06330
rosa_chinensis	RchiOBHm_Chr4g0413221 RchiOBHm_Chr7g0199651 RchiOBHm_Chr7g0199691 RchiOBHm_Chr7g0199711 RchiOBHm_Chr7g0199731
rosa_laevigata	RLG00000003775 RLG00000003776 RLG00000003777 RLG00000003778 RLG00000003779
rosa_multiflora	Rmu_co8400099.1_g000001 Rmu_sc0002748.1_g000025 Rmu_sc0002748.1_g000027 Rmu_sc0002748.1_g000028 Rmu_sc0009191.1_g000004
rosa_roxburghii	Rroxscaffold_3G00256480 Rroxscaffold_3G00256490 Rroxscaffold_3G00256520
rosa_rugosa	Rorug07G0055600 Rorug07G0055800 Rorug07G0055900
rosa_samantha	Rh7AG180700 Rh7AG181000 Rh7AG181200 Rh7BG183600 Rh7BG183900 Rh7BG184100 Rh7CG190400 Rh7CG190600 Rh7CG190700 Rh7DG183600 Rh7DG183800
rosa_wichuraiana	Rw7G015870 Rw7G015880 Rw7G015890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	558, 1224
AccB1I	GGYRCC	1 cut(s)	2588
AccB7I	CCANNNNNTGG	1 cut(s)	2501
AccI	GTMKAC	2 cut(s)	530, 1627
AccII	CGCG	3 cut(s)	536, 564, 2236
AccIII	TCCGGA	1 cut(s)	1171
AciI	CCGC	4 cut(s)	534, 564, 627, 1658
AclWI	GGATC	6 cut(s)	572, 899, 1708, 1841, 2155, 2163
AcoI	YGGCCR	2 cut(s)	3, 1835
AcsI	RAATTY	5 cut(s)	1446, 1455, 1717, 1872, 1978
AcuI	CTGAAG	4 cut(s)	116, 875, 972, 2082
AcyI	GRCGYC	1 cut(s)	2589
AfaI	GTAC	7 cut(s)	292, 434, 604, 707, 1691, 1958, 2067
AfiI	CCNNNNNNNGG	8 cut(s)	55, 310, 1030, 1250, 1251, 1936, 2477, 2501
AflII	CTTAAG	1 cut(s)	701
AjnI	CCWGG	7 cut(s)	18, 117, 202, 499, 598, 1567, 2476
AjuI	GAANNNNNNNTTGG	2 cut(s)	1864, 1896
Alw21I	GWGCWC	1 cut(s)	1209
Alw26I	GTCTC	4 cut(s)	33, 207, 2186, 2528
Alw44I	GTGCAC	1 cut(s)	1205
AlwI	GGATC	6 cut(s)	572, 899, 1708, 1841, 2155, 2163
Aor13HI	TCCGGA	1 cut(s)	1171
AoxI	GGCC	6 cut(s)	3, 138, 753, 1835, 1926, 2468
ApaLI	GTGCAC	1 cut(s)	1205
ApeKI	GCWGC	8 cut(s)	199, 1264, 1304, 1333, 1634, 1885, 2030, 2048
ApoI	RAATTY	5 cut(s)	1446, 1455, 1717, 1872, 1978
Asp700I	GAANNNNTTC	2 cut(s)	72, 2346
AspLEI	GCGC	2 cut(s)	2236, 2591
AspS9I	GGNCC	7 cut(s)	114, 276, 595, 1191, 1298, 1927, 2468
AsuHPI	GGTGA	6 cut(s)	857, 1312, 1856, 2381, 2414, 2590
AvaII	GGWCC	5 cut(s)	114, 276, 595, 1191, 1298
AxyI	CCTNAGG	1 cut(s)	54
BaeGI	GKGCMC	2 cut(s)	1209, 2462
BalI	TGGCCA	2 cut(s)	5, 1837
BanI	GGYRCC	1 cut(s)	2588
BanII	GRGCYC	1 cut(s)	942
BbsI	GAAGAC	4 cut(s)	863, 966, 1358, 1569
Bbv12I	GWGCWC	1 cut(s)	1209
BbvI	GCAGC	8 cut(s)	186, 1276, 1316, 1345, 1646, 1897, 2017, 2060
BccI	CCATC	9 cut(s)	57, 659, 686, 1036, 1074, 1289, 1339, 1753, 2600
BciT130I	CCWGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
BciVI	GTATCC	2 cut(s)	2264, 2445
BclI	TGATCA	1 cut(s)	583
BcoDI	GTCTC	4 cut(s)	33, 207, 2186, 2528
BfaI	CTAG	1 cut(s)	618
BfoI	RGCGCY	1 cut(s)	2592
BfrI	CTTAAG	1 cut(s)	701
BfuI	GTATCC	2 cut(s)	2264, 2445
BisI	GCNGC	9 cut(s)	200, 1265, 1305, 1334, 1635, 1659, 1886, 2031, 2049
BlpI	GCTNAGC	2 cut(s)	423, 512
BlsI	GCNGC	9 cut(s)	201, 1266, 1306, 1335, 1636, 1660, 1887, 2032, 2050
BmcAI	AGTACT	4 cut(s)	292, 1691, 1958, 2067
Bme1390I	CCNGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
Bme18I	GGWCC	5 cut(s)	114, 276, 595, 1191, 1298
BmgT120I	GGNCC	7 cut(s)	114, 276, 595, 1191, 1298, 1927, 2468
BmiI	GGNNCC	9 cut(s)	91, 115, 116, 457, 596, 597, 1476, 2469, 2590
BmrFI	CCNGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
BmrI	ACTGGG	2 cut(s)	786, 2574
BmsI	GCATC	5 cut(s)	450, 640, 730, 1087, 1414
BmuI	ACTGGG	2 cut(s)	786, 2574
BpiI	GAAGAC	4 cut(s)	863, 966, 1358, 1569
BpmI	CTGGAG	3 cut(s)	522, 1590, 2401
Bpu1102I	GCTNAGC	2 cut(s)	423, 512
BpuEI	CTTGAG	4 cut(s)	1418, 1504, 2230, 2414
BsaBI	GATNNNNATC	2 cut(s)	582, 1763
BsaHI	GRCGYC	1 cut(s)	2589
BsaI	GGTCTC	2 cut(s)	33, 2186
BsaJI	CCNNGG	8 cut(s)	117, 203, 598, 1259, 1268, 1567, 1990, 2172
BsaWI	WCCGGW	2 cut(s)	1171, 2437
Bsc4I	CCNNNNNNNGG	8 cut(s)	55, 310, 1030, 1250, 1251, 1936, 2477, 2501
Bse1I	ACTGG	5 cut(s)	480, 781, 1687, 2384, 2569
Bse21I	CCTNAGG	1 cut(s)	54
Bse3DI	GCAATG	2 cut(s)	1122, 2208
Bse8I	GATNNNNATC	2 cut(s)	582, 1763
BseAI	TCCGGA	1 cut(s)	1171
BseBI	CCWGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
BseDI	CCNNGG	8 cut(s)	117, 203, 598, 1259, 1268, 1567, 1990, 2172
BseJI	GATNNNNATC	2 cut(s)	582, 1763
BseLI	CCNNNNNNNGG	8 cut(s)	55, 310, 1030, 1250, 1251, 1936, 2477, 2501
BseMI	GCAATG	2 cut(s)	1122, 2208
BseMII	CTCAG	5 cut(s)	68, 432, 437, 503, 2087
BseNI	ACTGG	5 cut(s)	480, 781, 1687, 2384, 2569
BseRI	GAGGAG	2 cut(s)	1425, 2417
BseSI	GKGCMC	2 cut(s)	1209, 2462
BseXI	GCAGC	8 cut(s)	186, 1276, 1316, 1345, 1646, 1897, 2017, 2060
BseYI	CCCAGC	1 cut(s)	1029
BsgI	GTGCAG	1 cut(s)	2067
Bsh1236I	CGCG	3 cut(s)	536, 564, 2236
BshFI	GGCC	6 cut(s)	5, 140, 755, 1837, 1928, 2470
BshNI	GGYRCC	1 cut(s)	2588
BsiHKAI	GWGCWC	1 cut(s)	1209
BsiSI	CCGG	2 cut(s)	1172, 2438
BslFI	GGGAC	5 cut(s)	100, 220, 581, 914, 1170
BslI	CCNNNNNNNGG	8 cut(s)	55, 310, 1030, 1250, 1251, 1936, 2477, 2501
BsmAI	GTCTC	4 cut(s)	33, 207, 2186, 2528
BsmFI	GGGAC	5 cut(s)	100, 220, 581, 914, 1170
BsmI	GAATGC	1 cut(s)	1177
BsnI	GGCC	6 cut(s)	5, 140, 755, 1837, 1928, 2470
Bso31I	GGTCTC	2 cut(s)	33, 2186
Bsp1286I	GDGCHC	3 cut(s)	942, 1209, 2462
Bsp13I	TCCGGA	1 cut(s)	1171
Bsp1720I	GCTNAGC	2 cut(s)	423, 512
Bsp19I	CCATGG	2 cut(s)	1268, 2172
BspACI	CCGC	4 cut(s)	534, 564, 627, 1658
BspANI	GGCC	6 cut(s)	5, 140, 755, 1837, 1928, 2470
BspCNI	CTCAG	5 cut(s)	67, 431, 436, 504, 2086
BspEI	TCCGGA	1 cut(s)	1171
BspFNI	CGCG	3 cut(s)	536, 564, 2236
BspLI	GGNNCC	9 cut(s)	91, 115, 116, 457, 596, 597, 1476, 2469, 2590
BspPI	GGATC	6 cut(s)	572, 899, 1708, 1841, 2155, 2163
BspT107I	GGYRCC	1 cut(s)	2588
BspTI	CTTAAG	1 cut(s)	701
BspTNI	GGTCTC	2 cut(s)	33, 2186
BsrDI	GCAATG	2 cut(s)	1122, 2208
BsrI	ACTGG	5 cut(s)	480, 781, 1687, 2384, 2569
BssECI	CCNNGG	8 cut(s)	117, 203, 598, 1259, 1268, 1567, 1990, 2172
BssNAI	GTATAC	2 cut(s)	531, 1628
BssNI	GRCGYC	1 cut(s)	2589
BssT1I	CCWWGG	4 cut(s)	1259, 1268, 1990, 2172
Bst1107I	GTATAC	2 cut(s)	531, 1628
Bst2UI	CCWGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
Bst4CI	ACNGT	7 cut(s)	377, 1081, 1204, 1286, 1961, 2286, 2464
Bst6I	CTCTTC	4 cut(s)	281, 991, 1869, 2478
BstACI	GRCGYC	1 cut(s)	2589
BstAFI	CTTAAG	1 cut(s)	701
BstAPI	GCANNNNNTGC	2 cut(s)	1279, 1655
BstC8I	GCNNGC	2 cut(s)	173, 757
BstDEI	CTNAG	8 cut(s)	54, 393, 418, 423, 512, 574, 2073, 2582
BstDSI	CCRYGG	2 cut(s)	1268, 2172
BstEII	GGTNACC	1 cut(s)	2420
BstENI	CCTNNNNNAGG	2 cut(s)	1934, 2475
BstFNI	CGCG	3 cut(s)	536, 564, 2236
BstH2I	RGCGCY	1 cut(s)	2592
BstHHI	GCGC	2 cut(s)	2236, 2591
BstMAI	GTCTC	4 cut(s)	33, 207, 2186, 2528
BstMWI	GCNNNNNNNGC	8 cut(s)	181, 269, 344, 570, 1270, 1279, 1655, 2207
BstNI	CCWGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
BstNSI	RCATGY	2 cut(s)	188, 1634
BstPI	GGTNACC	1 cut(s)	2420
BstSCI	CCNGG	7 cut(s)	18, 117, 202, 499, 598, 1567, 2476
BstSLI	GKGCMC	2 cut(s)	1209, 2462
BstUI	CGCG	3 cut(s)	536, 564, 2236
BstV1I	GCAGC	8 cut(s)	186, 1276, 1316, 1345, 1646, 1897, 2017, 2060
BstV2I	GAAGAC	4 cut(s)	863, 966, 1358, 1569
BstX2I	RGATCY	2 cut(s)	577, 2147
BstYI	RGATCY	2 cut(s)	577, 2147
BstZ17I	GTATAC	2 cut(s)	531, 1628
Bsu36I	CCTNAGG	1 cut(s)	54
BsuI	GTATCC	2 cut(s)	2264, 2445
BsuRI	GGCC	6 cut(s)	5, 140, 755, 1837, 1928, 2470
BtgI	CCRYGG	2 cut(s)	1268, 2172
BtgZI	GCGATG	1 cut(s)	2544
BtsIMutI	CAGTG	8 cut(s)	382, 487, 785, 1209, 1551, 2282, 2469, 2562
Cac8I	GCNNGC	2 cut(s)	173, 757
CfoI	GCGC	2 cut(s)	2236, 2591
Cfr13I	GGNCC	7 cut(s)	114, 276, 595, 1191, 1298, 1927, 2468
CseI	GACGC	2 cut(s)	971, 1100
Csp6I	GTAC	7 cut(s)	291, 433, 603, 706, 1690, 1957, 2066
CviQI	GTAC	7 cut(s)	291, 433, 603, 706, 1690, 1957, 2066
DdeI	CTNAG	8 cut(s)	54, 393, 418, 423, 512, 574, 2073, 2582
DinI	GGCGCC	1 cut(s)	2590
DraI	TTTAAA	1 cut(s)	928
EaeI	YGGCCR	2 cut(s)	3, 1835
Eam1104I	CTCTTC	4 cut(s)	281, 991, 1869, 2478
EarI	CTCTTC	4 cut(s)	281, 991, 1869, 2478
Eco130I	CCWWGG	4 cut(s)	1259, 1268, 1990, 2172
Eco147I	AGGCCT	1 cut(s)	755
Eco24I	GRGCYC	1 cut(s)	942
Eco31I	GGTCTC	2 cut(s)	33, 2186
Eco47I	GGWCC	5 cut(s)	114, 276, 595, 1191, 1298
Eco57I	CTGAAG	4 cut(s)	116, 875, 972, 2082
Eco81I	CCTNAGG	1 cut(s)	54
Eco91I	GGTNACC	1 cut(s)	2420
EcoNI	CCTNNNNNAGG	2 cut(s)	1934, 2475
EcoO109I	RGGNCCY	5 cut(s)	114, 276, 595, 1927, 2468
EcoO65I	GGTNACC	1 cut(s)	2420
EcoRI	GAATTC	2 cut(s)	1717, 1872
EcoRII	CCWGG	7 cut(s)	18, 117, 202, 499, 598, 1567, 2476
EcoT14I	CCWWGG	4 cut(s)	1259, 1268, 1990, 2172
EcoT22I	ATGCAT	2 cut(s)	948, 1429
EcoT38I	GRGCYC	1 cut(s)	942
EgeI	GGCGCC	1 cut(s)	2590
EheI	GGCGCC	1 cut(s)	2590
ErhI	CCWWGG	4 cut(s)	1259, 1268, 1990, 2172
FalI	AAGNNNNNCTT	2 cut(s)	624, 656
FaqI	GGGAC	5 cut(s)	100, 220, 581, 914, 1170
FauNDI	CATATG	4 cut(s)	79, 125, 238, 1639
FbaI	TGATCA	1 cut(s)	583
FblI	GTMKAC	2 cut(s)	530, 1627
Fnu4HI	GCNGC	9 cut(s)	200, 1265, 1305, 1334, 1635, 1659, 1886, 2031, 2049
FriOI	GRGCYC	1 cut(s)	942
Fsp4HI	GCNGC	9 cut(s)	200, 1265, 1305, 1334, 1635, 1659, 1886, 2031, 2049
FspBI	CTAG	1 cut(s)	618
GlaI	GCGC	2 cut(s)	2235, 2590
GluI	GCNGC	9 cut(s)	200, 1265, 1305, 1334, 1635, 1659, 1886, 2031, 2049
GsaI	CCCAGC	1 cut(s)	1033
GsuI	CTGGAG	3 cut(s)	522, 1590, 2401
HaeII	RGCGCY	1 cut(s)	2592
HaeIII	GGCC	6 cut(s)	5, 140, 755, 1837, 1928, 2470
HapII	CCGG	2 cut(s)	1172, 2438
HgaI	GACGC	2 cut(s)	971, 1100
HhaI	GCGC	2 cut(s)	2236, 2591
Hin1I	GRCGYC	1 cut(s)	2589
Hin6I	GCGC	2 cut(s)	2234, 2589
HinP1I	GCGC	2 cut(s)	2234, 2589
HindIII	AAGCTT	2 cut(s)	686, 1503
HinfI	GANTC	9 cut(s)	23, 414, 983, 1535, 1944, 2335, 2383, 2521, 2545
HpaII	CCGG	2 cut(s)	1172, 2438
HphI	GGTGA	6 cut(s)	857, 1312, 1856, 2381, 2414, 2590
Hpy166II	GTNNAC	7 cut(s)	358, 531, 1090, 1207, 1628, 1964, 2252
Hpy188I	TCNGA	9 cut(s)	803, 813, 832, 1074, 1191, 1723, 1773, 2076, 2186
Hpy8I	GTNNAC	7 cut(s)	358, 531, 1090, 1207, 1628, 1964, 2252
Hpy99I	CGWCG	1 cut(s)	972
HpyAV	CCTTC	8 cut(s)	91, 158, 469, 929, 1835, 2012, 2171, 2410
HpyCH4III	ACNGT	7 cut(s)	377, 1081, 1204, 1286, 1961, 2286, 2464
HpyCH4IV	ACGT	3 cut(s)	970, 1159, 2499
HpyF10VI	GCNNNNNNNGC	8 cut(s)	181, 269, 344, 570, 1270, 1279, 1655, 2207
HpyF3I	CTNAG	8 cut(s)	54, 393, 418, 423, 512, 574, 2073, 2582
HpySE526I	ACGT	3 cut(s)	970, 1159, 2499
Hsp92I	GRCGYC	1 cut(s)	2589
HspAI	GCGC	2 cut(s)	2234, 2589
KasI	GGCGCC	1 cut(s)	2588
KflI	GGGWCCC	2 cut(s)	114, 595
Kpn2I	TCCGGA	1 cut(s)	1171
Ksp22I	TGATCA	1 cut(s)	583
LmnI	GCTCC	2 cut(s)	313, 1174
Lsp1109I	GCAGC	8 cut(s)	186, 1276, 1316, 1345, 1646, 1897, 2017, 2060
LweI	GCATC	5 cut(s)	450, 640, 730, 1087, 1414
MaeI	CTAG	1 cut(s)	618
MaeII	ACGT	3 cut(s)	970, 1159, 2499
MaeIII	GTNAC	7 cut(s)	241, 634, 783, 864, 2280, 2404, 2420
MfeI	CAATTG	1 cut(s)	1530
MflI	RGATCY	2 cut(s)	577, 2147
MhlI	GDGCHC	3 cut(s)	942, 1209, 2462
MlsI	TGGCCA	2 cut(s)	5, 1837
MluNI	TGGCCA	2 cut(s)	5, 1837
Mly113I	GGCGCC	1 cut(s)	2589
MlyI	GAGTC	5 cut(s)	977, 1938, 2344, 2392, 2530
MmeI	TCCRAC	6 cut(s)	391, 932, 1114, 1921, 2477, 2510
Mox20I	TGGCCA	2 cut(s)	5, 1837
Mph1103I	ATGCAT	2 cut(s)	948, 1429
MroI	TCCGGA	1 cut(s)	1171
MroXI	GAANNNNTTC	2 cut(s)	72, 2346
MscI	TGGCCA	2 cut(s)	5, 1837
MseI	TTAA	4 cut(s)	702, 918, 927, 978
MslI	CAYNNNNRTG	6 cut(s)	324, 378, 951, 1212, 1829, 2411
Msp20I	TGGCCA	2 cut(s)	5, 1837
MspCI	CTTAAG	1 cut(s)	701
MspI	CCGG	2 cut(s)	1172, 2438
MspR9I	CCNGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
MunI	CAATTG	1 cut(s)	1530
Mva1269I	GAATGC	1 cut(s)	1177
MvaI	CCWGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
MvnI	CGCG	3 cut(s)	536, 564, 2236
MwoI	GCNNNNNNNGC	8 cut(s)	181, 269, 344, 570, 1270, 1279, 1655, 2207
NarI	GGCGCC	1 cut(s)	2589
NcoI	CCATGG	2 cut(s)	1268, 2172
NdeI	CATATG	4 cut(s)	79, 125, 238, 1639
NlaIV	GGNNCC	9 cut(s)	91, 115, 116, 457, 596, 597, 1476, 2469, 2590
NmeAIII	GCCGAG	1 cut(s)	2505
NmuCI	GTSAC	5 cut(s)	783, 864, 2280, 2404, 2420
NsiI	ATGCAT	2 cut(s)	948, 1429
NspI	RCATGY	2 cut(s)	188, 1634
PceI	AGGCCT	1 cut(s)	755
PctI	GAATGC	1 cut(s)	1177
PdmI	GAANNNNTTC	2 cut(s)	72, 2346
PfeI	GAWTC	4 cut(s)	23, 414, 1535, 2545
PflFI	GACNNNGTC	1 cut(s)	862
PflMI	CCANNNNNTGG	1 cut(s)	2501
PfoI	TCCNGGA	1 cut(s)	2476
PkrI	GCNGC	9 cut(s)	201, 1266, 1306, 1335, 1636, 1660, 1887, 2032, 2050
PleI	GAGTC	5 cut(s)	977, 1938, 2343, 2391, 2529
PluTI	GGCGCC	1 cut(s)	2592
PpsI	GAGTC	5 cut(s)	977, 1938, 2343, 2391, 2529
PpuMI	RGGWCCY	3 cut(s)	114, 276, 595
PsiI	TTATAA	2 cut(s)	558, 1224
Psp5II	RGGWCCY	3 cut(s)	114, 276, 595
Psp6I	CCWGG	7 cut(s)	18, 117, 202, 499, 598, 1567, 2476
PspEI	GGTNACC	1 cut(s)	2420
PspFI	CCCAGC	1 cut(s)	1029
PspGI	CCWGG	7 cut(s)	18, 117, 202, 499, 598, 1567, 2476
PspN4I	GGNNCC	9 cut(s)	91, 115, 116, 457, 596, 597, 1476, 2469, 2590
PspPI	GGNCC	7 cut(s)	114, 276, 595, 1191, 1298, 1927, 2468
PspPPI	RGGWCCY	3 cut(s)	114, 276, 595
PsuI	RGATCY	2 cut(s)	577, 2147
PsyI	GACNNNGTC	1 cut(s)	862
RsaI	GTAC	7 cut(s)	292, 434, 604, 707, 1691, 1958, 2067
RsaNI	GTAC	7 cut(s)	291, 433, 603, 706, 1690, 1957, 2066
RseI	CAYNNNNRTG	6 cut(s)	324, 378, 951, 1212, 1829, 2411
SaqAI	TTAA	4 cut(s)	702, 918, 927, 978
SatI	GCNGC	9 cut(s)	200, 1265, 1305, 1334, 1635, 1659, 1886, 2031, 2049
Sau96I	GGNCC	7 cut(s)	114, 276, 595, 1191, 1298, 1927, 2468
ScaI	AGTACT	4 cut(s)	292, 1691, 1958, 2067
SchI	GAGTC	5 cut(s)	977, 1938, 2344, 2392, 2530
ScrFI	CCNGG	7 cut(s)	20, 119, 204, 501, 600, 1569, 2478
SduI	GDGCHC	3 cut(s)	942, 1209, 2462
SfaNI	GCATC	5 cut(s)	450, 640, 730, 1087, 1414
SfoI	GGCGCC	1 cut(s)	2590
SinI	GGWCC	5 cut(s)	114, 276, 595, 1191, 1298
SmiMI	CAYNNNNRTG	6 cut(s)	324, 378, 951, 1212, 1829, 2411
SmlI	CTYRAG	5 cut(s)	701, 1433, 1483, 2245, 2429
SmoI	CTYRAG	5 cut(s)	701, 1433, 1483, 2245, 2429
SseBI	AGGCCT	1 cut(s)	755
SsiI	CCGC	4 cut(s)	534, 564, 627, 1658
SspDI	GGCGCC	1 cut(s)	2588
SspMI	CTAG	1 cut(s)	618
StuI	AGGCCT	1 cut(s)	755
StyD4I	CCNGG	7 cut(s)	18, 117, 202, 499, 598, 1567, 2476
StyI	CCWWGG	4 cut(s)	1259, 1268, 1990, 2172
TaaI	ACNGT	7 cut(s)	377, 1081, 1204, 1286, 1961, 2286, 2464
TaiI	ACGT	3 cut(s)	973, 1162, 2502
TaqI	TCGA	5 cut(s)	297, 967, 1012, 1238, 1947
TatI	WGTACW	5 cut(s)	290, 432, 1689, 1956, 2065
TauI	GCSGC	1 cut(s)	1661
TfiI	GAWTC	4 cut(s)	23, 414, 1535, 2545
Tru1I	TTAA	4 cut(s)	702, 918, 927, 978
Tru9I	TTAA	4 cut(s)	702, 918, 927, 978
TscAI	CASTG	8 cut(s)	382, 487, 792, 1209, 1551, 2289, 2469, 2569
TseFI	GTSAC	5 cut(s)	783, 864, 2280, 2404, 2420
TseI	GCWGC	8 cut(s)	199, 1264, 1304, 1333, 1634, 1885, 2030, 2048
Tsp45I	GTSAC	5 cut(s)	783, 864, 2280, 2404, 2420
TspDTI	ATGAA	8 cut(s)	816, 972, 1601, 1926, 2145, 2415, 2429, 2555
TspGWI	ACGGA	4 cut(s)	1213, 1514, 1643, 2271
TspRI	CASTG	8 cut(s)	382, 487, 792, 1209, 1551, 2289, 2469, 2569
Tth111I	GACNNNGTC	1 cut(s)	862
Van91I	CCANNNNNTGG	1 cut(s)	2501
Vha464I	CTTAAG	1 cut(s)	701
VneI	GTGCAC	1 cut(s)	1205
VpaK11BI	GGWCC	5 cut(s)	114, 276, 595, 1191, 1298
XagI	CCTNNNNNAGG	2 cut(s)	1934, 2475
XapI	RAATTY	5 cut(s)	1446, 1455, 1717, 1872, 1978
XceI	RCATGY	2 cut(s)	188, 1634
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1266, 1694
XmiI	GTMKAC	2 cut(s)	530, 1627
XmnI	GAANNNNTTC	2 cut(s)	72, 2346
XspI	CTAG	1 cut(s)	618
ZrmI	AGTACT	4 cut(s)	292, 1691, 1958, 2067
Zsp2I	ATGCAT	2 cut(s)	948, 1429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.