Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001557

Physical Location & Seq

Genomic Coordinates Forward (+)

50885288 .. 50892437

Gene Structure 7150 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00256520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2559 bp

ATGGCTAGGCGTTACGCAGAATCGTTGTCTTTTGGAGGGTTGTCGTTTGGAGGGTCGGGACATATTCAGGGGCAACAACTTGTGCCGTTTCGGACAGGCAGTCTATCTCGGAAGCTTGAGCTACTCCATGGGAACTTGGTTATATTGGTCAAGGAGGCCAAAAACCTTCCTAACATGGATGCGTTTCACAAGACTCTGGGGGATATTGTCGCAAGATTGCCTGGGACTGGTAACAACAAGATAACGAGTGATCCGTATGTCACCGTGTCGGTTGCAGGTGCTGTAGTTGGGAGGACTTTTGTGATCAGTAATAGTGAGAACCCTGTTTGGGAGCAACATTTTGATGTTCCGGTTGCGCATTATGCTTCAGAAGTGCAATTTGTTGTCAAAGATAGTGATGTTGTGGGTTCCCAGCTCATAGGTGTTGTAGCAATCCCAGTTGAGCGGGTATATTCTGGGATGAAAGTTGAGGGCACCTTTCCAATCCTTGGTTCCAATGGGAAGCCCTGTAAGCCTGGAGCTGTTTTGACCTTATCGATTCAGTATGTTCCTATTGAAAAATTGACCTTTTATGATAATGGGGTAGGTTCAGATCCTGATCATCAAGGGGTTCCTGGTACTTACTTTCCTGTTAGGAAAGGCGGTAAAGTTACTCTGTATCAAGATGCCCATGTTCCTGATGGTTGCCTTCCAAGTATCAAGCTTGACGGCAATGTTGAGTACGAGCATGGGAGTTGTTGGCAAGACATATTTGATGCAATTAGTCAGGCCCGTCGTTTGATATACATTGTGGGGTGGTCGGTGTACCACAAAGTTAGGTTGCTCCGGGATAATGGTAGAACAGATTGCATATTAGGTGATCTTCTTAAGGACAAGTCACAGGAAGGTGTGAGAGTGTTGCTTCTTGTTTGGGATGATCCAACTTCAAGGAGCATTATGGGATATAAAACGGATGGAATTATGCAAACCAATGATGAGGAAACTCGCCGTTTCTTTAAGCATTCTTCAGTCCAAGTGCTACTTTGCCCTCGATCAGCTGGAAAGGGTAGCTGGGCCAAAAAGCAGGAAGTCGGAACAATCTACACCCACCATCAGAAAACAGTGCTTGTTGATGCTGATGCAGGTCATTCTAAAAGAAAGATCATTGCATTTGTTGGTGGTCTTGATTTGTGCCTGGGGCGCTACGATACTCCAGAGCATCCTATTTTTAGGACTTTACAGACAGTCCACAAGGATGACAATCGTAACCCTACTTTTTCGGACCGCGTTGTTGGTTGTCCAAGGCAGCCATGGCATGATTTGCACAGTAAAATTGAGGGTCCAGCAGCATACGATATCCTTACCAATTTTCAGGAGCGTTGGTTAAAAGCTTCAAAACCACGTGGGATGCAGAAACTAAAAGCACTACATGATGATGCATTACTGAAGATTGAGAGGATCCCGGAAATTATGGGGATGGCAGATCTTCCTCAACTGAGTGAGGATGATTTAGAAGCTTGGGATGTTCAGGTTTTTCGTTCAATTGATTCCAATTCTGTGAGAGGATTTCCAGAAGACCCAAAAGATGCTTCACGCATGAACCTGGTGTGTGGGAAGAATGTGCTGATAGACATGAGTATACATGCCGCATATGTCAAAGCAATTCGCGCTGCTCAACATTTCATTTACATAGAGAACCAGTACTTCCTTGGATCATCATATAATTGGACCTTATACAGTGATTTAGGTGCAAATAACTTGATACCAATGGAAATTGCTCTCAAAATTGTGAATAAAATTAAAGCAAACGAGAGGTTTTCTGTATATATTGTTATCCCCATGTGGCCGGAAGGTGTTCCTACAAGCAGTCCTCTTCAACGAATTCTCTGTTGGCAGCATAAAACGATGCAAATGATGTATGAAATGATTTACAAAGCTCTACAGGAGGCTGGACTTCACAATAAATATTGTCCACAAGACTATTTGAATTTCTTTTGCCTCGGTAATCGTGAAAAACCAAGTGGTGAAGAAAGTTCTGGTGCAAAAAAATCAACTGAAGCAAACAAAAAAGAAGCAAACACTCCTCAAGCACTCACTCGGAAGAACAGGCGCTTTATGATTTATGTCCATTCAAAAGGAATGATAGTGGACGATGAATATGTCATATTAGGATCTGCAAATATCAATCAGCGATCCATGGAAGGTACACGAGATACTGAAATAGCAATGGGGGCATATCAGCCTCATCATACCTGGGCGAGGAAACTCTCAAGTCCACACGGACAGATATATGGATATAGAACATCACTTTGGGCGGAGCATATTGGTTTTCTTGAAGACTCTTTCAACCAACCAGAGAGTCTCGAATGTGTGCAGCGGGTAAGATCTTTGAGCGAGCAGAACTGGAGGCAGTATGTTGCTGATGAGATTACAGAAATGAAGGGCCACCTGCTGAAATATCCGGTTGAAGTAGATCAAATGGGCAAGGTGAAGCATCTTGCAGGCTGTACAACATTTCCAGATGTTGGTGGCAACATACTTGGGGATTTTATAGCCATCCAAGAAAATCTCACCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005488 GO:0005543 GO:0005546 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0005911 GO:0006629 GO:0006643 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008289 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009816 GO:0009987 GO:0010035 GO:0010038 GO:0010044 GO:0010154 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0022414 GO:0022626 GO:0030054 GO:0032501 GO:0032502 GO:0032991 GO:0035091 GO:0042221 GO:0042578 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0045087 GO:0046686 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0061458 GO:0071704 GO:0071944 GO:0098542 GO:1901981 GO:1902936 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

852

Amino Acids

95.68

Weight (kDa)

7.17

Isoelectric Point (pI)

39.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	44 - 161	1.3e-16	C2 domain
PLDc_2	PF13091	676 - 736	1.5e-06	PLD-like domain
PLDc	PF00614	700 - 725	8.2e-08	Phospholipase D Active site motif
PLD_C	PF12357	772 - 842	2.7e-30	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11830 AT4G11830 AT4G11830 AT4G11830 AT4G11840 AT4G11840 AT4G11850
fragaria_vesca	FvH4_5g01274 FvH4_5g01290 FvH4_5g01290 FvH4_5g01290
malus_domestica	MD04G1069600.v1.1
prunus_persica	Prupe.5G088300_v2.0.a1 Prupe.5G088300_v2.0.a1
pyrus_communis	pycom04g06330
rosa_chinensis	RchiOBHm_Chr4g0413221 RchiOBHm_Chr7g0199651 RchiOBHm_Chr7g0199691 RchiOBHm_Chr7g0199711 RchiOBHm_Chr7g0199731
rosa_laevigata	RLG00000003775 RLG00000003776 RLG00000003777 RLG00000003778 RLG00000003779
rosa_multiflora	Rmu_co8400099.1_g000001 Rmu_sc0002748.1_g000025 Rmu_sc0002748.1_g000027 Rmu_sc0002748.1_g000028 Rmu_sc0009191.1_g000004
rosa_roxburghii	Rroxscaffold_3G00256480 Rroxscaffold_3G00256490 Rroxscaffold_3G00256520
rosa_rugosa	Rorug07G0055600 Rorug07G0055800 Rorug07G0055900
rosa_samantha	Rh7AG180700 Rh7AG181000 Rh7AG181200 Rh7BG183600 Rh7BG183900 Rh7BG184100 Rh7CG190400 Rh7CG190600 Rh7CG190700 Rh7DG183600 Rh7DG183800
rosa_wichuraiana	Rw7G015870 Rw7G015880 Rw7G015890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	266, 2436
Acc16I	TGCGCA	1 cut(s)	357
Acc36I	ACCTGC	3 cut(s)	266, 1112, 2436
AccB1I	GGYRCC	1 cut(s)	473
AccB7I	CCANNNNNTGG	2 cut(s)	488, 2504
AccBSI	CCGCTC	1 cut(s)	445
AccI	GTMKAC	1 cut(s)	1618
AccII	CGCG	2 cut(s)	1266, 1647
AciI	CCGC	6 cut(s)	445, 642, 1264, 1626, 2294, 2356
AclWI	GGATC	8 cut(s)	245, 587, 911, 1432, 1445, 1699, 2158, 2166
AcoI	YGGCCR	1 cut(s)	1823
AcsI	RAATTY	2 cut(s)	1860, 1966
AcuI	CTGAAG	4 cut(s)	351, 990, 1445, 2055
AcvI	CACGTG	1 cut(s)	1382
AfaI	GTAC	6 cut(s)	619, 722, 806, 1682, 2185, 2488
AfiI	CCNNNNNNNGG	5 cut(s)	227, 328, 488, 2238, 2504
AflII	CTTAAG	1 cut(s)	866
AhdI	GACNNNNNGTC	1 cut(s)	99
AjnI	CCWGG	6 cut(s)	220, 514, 613, 1173, 1581, 2231
AjuI	GAANNNNNNNTTGG	8 cut(s)	475, 507, 1552, 1584, 1852, 1884, 2272, 2304
Alw26I	GTCTC	1 cut(s)	2345
AlwI	GGATC	8 cut(s)	245, 587, 911, 1432, 1445, 1699, 2158, 2166
AlwNI	CAGNNNCTG	2 cut(s)	281, 596
AoxI	GGCC	5 cut(s)	156, 768, 1053, 1823, 2422
ApeKI	GCWGC	5 cut(s)	1285, 1325, 1649, 1873, 2353
ApoI	RAATTY	2 cut(s)	1860, 1966
Asp700I	GAANNNNTTC	1 cut(s)	1833
AspLEI	GCGC	4 cut(s)	358, 1182, 1649, 2091
AspS9I	GGNCC	6 cut(s)	769, 1053, 1261, 1319, 1707, 2422
AsuC2I	CCSGG	2 cut(s)	827, 1442
AsuHPI	GGTGA	5 cut(s)	253, 869, 2015, 2479, 2542
AvaII	GGWCC	3 cut(s)	1261, 1319, 1707
BaeGI	GKGCMC	1 cut(s)	476
BamHI	GGATCC	1 cut(s)	1437
BanI	GGYRCC	1 cut(s)	473
BauI	CACGAG	1 cut(s)	2187
BbrPI	CACGTG	1 cut(s)	1382
BbsI	GAAGAC	2 cut(s)	1560, 2322
BbvI	GCAGC	5 cut(s)	1297, 1337, 1636, 1885, 2365
BccI	CCATC	5 cut(s)	674, 947, 1098, 1450, 2543
BceAI	ACGGC	3 cut(s)	70, 724, 972
BciT130I	CCWGG	6 cut(s)	222, 516, 615, 1175, 1583, 2233
BclI	TGATCA	2 cut(s)	303, 598
BcnI	CCSGG	2 cut(s)	827, 1442
BcoDI	GTCTC	1 cut(s)	2345
BfaI	CTAG	1 cut(s)	6
BfmI	CTRYAG	2 cut(s)	282, 1919
BfoI	RGCGCY	2 cut(s)	1183, 2092
BfrI	CTTAAG	1 cut(s)	866
BfuAI	ACCTGC	3 cut(s)	266, 1112, 2436
BglII	AGATCT	2 cut(s)	1462, 2363
BisI	GCNGC	6 cut(s)	1286, 1326, 1626, 1650, 1874, 2354
BlsI	GCNGC	6 cut(s)	1287, 1327, 1627, 1651, 1875, 2355
BmcAI	AGTACT	1 cut(s)	1682
Bme1390I	CCNGG	8 cut(s)	222, 516, 615, 827, 1175, 1442, 1583, 2233
Bme18I	GGWCC	3 cut(s)	1261, 1319, 1707
BmeRI	GACNNNNNGTC	1 cut(s)	99
BmgT120I	GGNCC	6 cut(s)	769, 1053, 1261, 1319, 1707, 2422
BmiI	GGNNCC	6 cut(s)	409, 475, 493, 612, 1320, 1439
BmrFI	CCNGG	8 cut(s)	222, 516, 615, 827, 1175, 1442, 1583, 2233
BmrI	ACTGGG	1 cut(s)	431
BmuI	ACTGGG	1 cut(s)	431
BpiI	GAAGAC	2 cut(s)	1560, 2322
BpmI	CTGGAG	3 cut(s)	537, 1176, 2404
BpuEI	CTTGAG	3 cut(s)	137, 2049, 2233
BpuMI	CCSGG	2 cut(s)	827, 1442
Bsa29I	ATCGAT	1 cut(s)	536
BsaAI	YACGTR	1 cut(s)	1382
BsaBI	GATNNNNATC	2 cut(s)	597, 1239
BsaWI	WCCGGW	2 cut(s)	349, 2440
BsaXI	ACNNNNNCTCC	2 cut(s)	1346, 1376
Bsc4I	CCNNNNNNNGG	5 cut(s)	227, 328, 488, 2238, 2504
Bse1I	ACTGG	4 cut(s)	232, 437, 1678, 2387
Bse3DI	GCAATG	3 cut(s)	718, 1143, 2211
Bse8I	GATNNNNATC	2 cut(s)	597, 1239
BseBI	CCWGG	6 cut(s)	222, 516, 615, 1175, 1583, 2233
BseCI	ATCGAT	1 cut(s)	536
BseJI	GATNNNNATC	2 cut(s)	597, 1239
BseLI	CCNNNNNNNGG	5 cut(s)	227, 328, 488, 2238, 2504
BseMI	GCAATG	3 cut(s)	718, 1143, 2211
BseMII	CTCAG	1 cut(s)	1466
BseNI	ACTGG	4 cut(s)	232, 437, 1678, 2387
BseRI	GAGGAG	1 cut(s)	2052
BseSI	GKGCMC	1 cut(s)	476
BseXI	GCAGC	5 cut(s)	1297, 1337, 1636, 1885, 2365
BseYI	CCCAGC	2 cut(s)	411, 1050
BsgI	GTGCAG	1 cut(s)	2372
Bsh1236I	CGCG	2 cut(s)	1266, 1647
BshFI	GGCC	5 cut(s)	158, 770, 1055, 1825, 2424
BshNI	GGYRCC	1 cut(s)	473
BshVI	ATCGAT	1 cut(s)	536
BsiSI	CCGG	5 cut(s)	350, 826, 1442, 1826, 2441
BslFI	GGGAC	2 cut(s)	72, 238
BslI	CCNNNNNNNGG	5 cut(s)	227, 328, 488, 2238, 2504
BsmAI	GTCTC	1 cut(s)	2345
BsmFI	GGGAC	2 cut(s)	72, 238
BsmI	GAATGC	1 cut(s)	1000
BsnI	GGCC	5 cut(s)	158, 770, 1055, 1825, 2424
Bsp1286I	GDGCHC	1 cut(s)	476
Bsp1407I	TGTACA	1 cut(s)	2486
Bsp19I	CCATGG	3 cut(s)	127, 1289, 2175
BspACI	CCGC	6 cut(s)	445, 642, 1264, 1626, 2294, 2356
BspANI	GGCC	5 cut(s)	158, 770, 1055, 1825, 2424
BspCNI	CTCAG	1 cut(s)	1467
BspDI	ATCGAT	1 cut(s)	536
BspFNI	CGCG	2 cut(s)	1266, 1647
BspLI	GGNNCC	6 cut(s)	409, 475, 493, 612, 1320, 1439
BspMI	ACCTGC	3 cut(s)	266, 1112, 2436
BspPI	GGATC	8 cut(s)	245, 587, 911, 1432, 1445, 1699, 2158, 2166
BspT107I	GGYRCC	1 cut(s)	473
BspTI	CTTAAG	1 cut(s)	866
BsrBI	CCGCTC	1 cut(s)	445
BsrDI	GCAATG	3 cut(s)	718, 1143, 2211
BsrGI	TGTACA	1 cut(s)	2486
BsrI	ACTGG	4 cut(s)	232, 437, 1678, 2387
BssNAI	GTATAC	1 cut(s)	1619
BssSI	CACGAG	1 cut(s)	2187
BssT1I	CCWWGG	6 cut(s)	127, 487, 1280, 1289, 1687, 2175
Bst1107I	GTATAC	1 cut(s)	1619
Bst2BI	CACGAG	1 cut(s)	2187
Bst2UI	CCWGG	6 cut(s)	222, 516, 615, 1175, 1583, 2233
Bst4CI	ACNGT	5 cut(s)	265, 1102, 1225, 1307, 1718
Bst6I	CTCTTC	1 cut(s)	1857
BstAFI	CTTAAG	1 cut(s)	866
BstAPI	GCANNNNNTGC	1 cut(s)	1300
BstAUI	TGTACA	1 cut(s)	2486
BstBAI	YACGTR	1 cut(s)	1382
BstC8I	GCNNGC	2 cut(s)	2375, 2482
BstDEI	CTNAG	1 cut(s)	1475
BstDSI	CCRYGG	3 cut(s)	127, 1289, 2175
BstENI	CCTNNNNNAGG	1 cut(s)	2236
BstFNI	CGCG	2 cut(s)	1266, 1647
BstH2I	RGCGCY	2 cut(s)	1183, 2092
BstHHI	GCGC	4 cut(s)	358, 1182, 1649, 2091
BstMAI	GTCTC	1 cut(s)	2345
BstMWI	GCNNNNNNNGC	7 cut(s)	362, 511, 1179, 1291, 1300, 1646, 2210
BstNI	CCWGG	6 cut(s)	222, 516, 615, 1175, 1583, 2233
BstNSI	RCATGY	1 cut(s)	1625
BstSCI	CCNGG	8 cut(s)	220, 514, 613, 825, 1173, 1440, 1581, 2231
BstSFI	CTRYAG	2 cut(s)	282, 1919
BstSLI	GKGCMC	1 cut(s)	476
BstUI	CGCG	2 cut(s)	1266, 1647
BstV1I	GCAGC	5 cut(s)	1297, 1337, 1636, 1885, 2365
BstV2I	GAAGAC	2 cut(s)	1560, 2322
BstX2I	RGATCY	5 cut(s)	592, 1437, 1462, 2150, 2363
BstYI	RGATCY	5 cut(s)	592, 1437, 1462, 2150, 2363
BstZ17I	GTATAC	1 cut(s)	1619
Bsu15I	ATCGAT	1 cut(s)	536
BsuRI	GGCC	5 cut(s)	158, 770, 1055, 1825, 2424
BsuTUI	ATCGAT	1 cut(s)	536
BtgI	CCRYGG	3 cut(s)	127, 1289, 2175
BtsIMutI	CAGTG	2 cut(s)	1107, 1723
BveI	ACCTGC	3 cut(s)	266, 1112, 2436
Cac8I	GCNNGC	2 cut(s)	2375, 2482
CaiI	CAGNNNCTG	2 cut(s)	281, 596
CfoI	GCGC	4 cut(s)	358, 1182, 1649, 2091
Cfr13I	GGNCC	6 cut(s)	769, 1053, 1261, 1319, 1707, 2422
ClaI	ATCGAT	1 cut(s)	536
CpoI	CGGWCCG	1 cut(s)	1261
CsiI	ACCWGGT	1 cut(s)	1581
Csp6I	GTAC	6 cut(s)	618, 721, 805, 1681, 2184, 2487
CspI	CGGWCCG	1 cut(s)	1261
CviQI	GTAC	6 cut(s)	618, 721, 805, 1681, 2184, 2487
DdeI	CTNAG	1 cut(s)	1475
DriI	GACNNNNNGTC	1 cut(s)	99
EaeI	YGGCCR	1 cut(s)	1823
Eam1104I	CTCTTC	1 cut(s)	1857
Eam1105I	GACNNNNNGTC	1 cut(s)	99
EarI	CTCTTC	1 cut(s)	1857
EciI	GGCGGA	1 cut(s)	2309
Eco130I	CCWWGG	6 cut(s)	127, 487, 1280, 1289, 1687, 2175
Eco32I	GATATC	1 cut(s)	1336
Eco47I	GGWCC	3 cut(s)	1261, 1319, 1707
Eco57I	CTGAAG	4 cut(s)	351, 990, 1445, 2055
Eco72I	CACGTG	1 cut(s)	1382
EcoNI	CCTNNNNNAGG	1 cut(s)	2236
EcoRI	GAATTC	1 cut(s)	1860
EcoRII	CCWGG	6 cut(s)	220, 514, 613, 1173, 1581, 2231
EcoRV	GATATC	1 cut(s)	1336
EcoT14I	CCWWGG	6 cut(s)	127, 487, 1280, 1289, 1687, 2175
EcoT22I	ATGCAT	1 cut(s)	1420
ErhI	CCWWGG	6 cut(s)	127, 487, 1280, 1289, 1687, 2175
FalI	AAGNNNNNCTT	2 cut(s)	1005, 1037
FaqI	GGGAC	2 cut(s)	72, 238
FauI	CCCGC	2 cut(s)	438, 2349
FauNDI	CATATG	1 cut(s)	1630
FbaI	TGATCA	2 cut(s)	303, 598
FblI	GTMKAC	1 cut(s)	1618
Fnu4HI	GCNGC	6 cut(s)	1286, 1326, 1626, 1650, 1874, 2354
Fsp4HI	GCNGC	6 cut(s)	1286, 1326, 1626, 1650, 1874, 2354
FspBI	CTAG	1 cut(s)	6
FspI	TGCGCA	1 cut(s)	357
GlaI	GCGC	4 cut(s)	357, 1181, 1648, 2090
GluI	GCNGC	6 cut(s)	1286, 1326, 1626, 1650, 1874, 2354
GsaI	CCCAGC	2 cut(s)	415, 1054
GsuI	CTGGAG	3 cut(s)	537, 1176, 2404
HaeII	RGCGCY	2 cut(s)	1183, 2092
HaeIII	GGCC	5 cut(s)	158, 770, 1055, 1825, 2424
HapII	CCGG	5 cut(s)	350, 826, 1442, 1826, 2441
HhaI	GCGC	4 cut(s)	358, 1182, 1649, 2091
Hin6I	GCGC	4 cut(s)	356, 1180, 1647, 2089
HinP1I	GCGC	4 cut(s)	356, 1180, 1647, 2089
HindIII	AAGCTT	4 cut(s)	113, 701, 1368, 1494
HinfI	GANTC	6 cut(s)	20, 193, 538, 1526, 2318, 2338
HpaII	CCGG	5 cut(s)	350, 826, 1442, 1826, 2441
HphI	GGTGA	5 cut(s)	253, 869, 2015, 2479, 2542
Hpy166II	GTNNAC	7 cut(s)	805, 1228, 1619, 1952, 2128, 2186, 2255
Hpy188I	TCNGA	9 cut(s)	93, 111, 370, 592, 1073, 1095, 1261, 2079, 2558
Hpy8I	GTNNAC	7 cut(s)	805, 1228, 1619, 1952, 2128, 2186, 2255
Hpy99I	CGWCG	1 cut(s)	777
HpyAV	CCTTC	6 cut(s)	176, 698, 878, 1823, 2174, 2413
HpyCH4III	ACNGT	5 cut(s)	265, 1102, 1225, 1307, 1718
HpyCH4IV	ACGT	1 cut(s)	1381
HpyF10VI	GCNNNNNNNGC	7 cut(s)	362, 511, 1179, 1291, 1300, 1646, 2210
HpyF3I	CTNAG	1 cut(s)	1475
HpySE526I	ACGT	1 cut(s)	1381
HspAI	GCGC	4 cut(s)	356, 1180, 1647, 2089
Ksp22I	TGATCA	2 cut(s)	303, 598
LmnI	GCTCC	6 cut(s)	331, 518, 828, 930, 1354, 2296
Lsp1109I	GCAGC	5 cut(s)	1297, 1337, 1636, 1885, 2365
MabI	ACCWGGT	1 cut(s)	1581
MaeI	CTAG	1 cut(s)	6
MaeII	ACGT	1 cut(s)	1381
MaeIII	GTNAC	6 cut(s)	11, 230, 259, 649, 876, 1244
MbiI	CCGCTC	1 cut(s)	445
MfeI	CAATTG	1 cut(s)	1521
MflI	RGATCY	5 cut(s)	592, 1437, 1462, 2150, 2363
MhlI	GDGCHC	1 cut(s)	476
MlyI	GAGTC	3 cut(s)	187, 2312, 2347
MmeI	TCCRAC	2 cut(s)	944, 1051
Mph1103I	ATGCAT	1 cut(s)	1420
MroXI	GAANNNNTTC	1 cut(s)	1833
MseI	TTAA	4 cut(s)	867, 996, 1364, 1779
MslI	CAYNNNNRTG	3 cut(s)	342, 1233, 1413
MspA1I	CMGCKG	2 cut(s)	1037, 2356
MspCI	CTTAAG	1 cut(s)	866
MspI	CCGG	5 cut(s)	350, 826, 1442, 1826, 2441
MspR9I	CCNGG	8 cut(s)	222, 516, 615, 827, 1175, 1442, 1583, 2233
MunI	CAATTG	1 cut(s)	1521
Mva1269I	GAATGC	1 cut(s)	1000
MvaI	CCWGG	6 cut(s)	222, 516, 615, 1175, 1583, 2233
MvnI	CGCG	2 cut(s)	1266, 1647
MwoI	GCNNNNNNNGC	7 cut(s)	362, 511, 1179, 1291, 1300, 1646, 2210
NciI	CCSGG	2 cut(s)	827, 1442
NcoI	CCATGG	3 cut(s)	127, 1289, 2175
NdeI	CATATG	1 cut(s)	1630
NlaIV	GGNNCC	6 cut(s)	409, 475, 493, 612, 1320, 1439
NmuCI	GTSAC	2 cut(s)	259, 876
NsbI	TGCGCA	1 cut(s)	357
NsiI	ATGCAT	1 cut(s)	1420
NspI	RCATGY	1 cut(s)	1625
PaqCI	CACCTGC	2 cut(s)	266, 2436
PcsI	WCGNNNNNNNCGW	1 cut(s)	251
PctI	GAATGC	1 cut(s)	1000
PdmI	GAANNNNTTC	1 cut(s)	1833
PfeI	GAWTC	3 cut(s)	20, 538, 1526
PflMI	CCANNNNNTGG	2 cut(s)	488, 2504
PfoI	TCCNGGA	2 cut(s)	825, 1440
PkrI	GCNGC	6 cut(s)	1287, 1327, 1627, 1651, 1875, 2355
PleI	GAGTC	3 cut(s)	187, 2312, 2346
PmaCI	CACGTG	1 cut(s)	1382
PmlI	CACGTG	1 cut(s)	1382
PpsI	GAGTC	3 cut(s)	187, 2312, 2346
Ppu21I	YACGTR	1 cut(s)	1382
Psp6I	CCWGG	6 cut(s)	220, 514, 613, 1173, 1581, 2231
PspCI	CACGTG	1 cut(s)	1382
PspFI	CCCAGC	2 cut(s)	411, 1050
PspGI	CCWGG	6 cut(s)	220, 514, 613, 1173, 1581, 2231
PspN4I	GGNNCC	6 cut(s)	409, 475, 493, 612, 1320, 1439
PspPI	GGNCC	6 cut(s)	769, 1053, 1261, 1319, 1707, 2422
PstNI	CAGNNNCTG	2 cut(s)	281, 596
PsuI	RGATCY	5 cut(s)	592, 1437, 1462, 2150, 2363
PvuII	CAGCTG	1 cut(s)	1037
RsaI	GTAC	6 cut(s)	619, 722, 806, 1682, 2185, 2488
RsaNI	GTAC	6 cut(s)	618, 721, 805, 1681, 2184, 2487
RseI	CAYNNNNRTG	3 cut(s)	342, 1233, 1413
Rsr2I	CGGWCCG	1 cut(s)	1261
RsrII	CGGWCCG	1 cut(s)	1261
SaqAI	TTAA	4 cut(s)	867, 996, 1364, 1779
SatI	GCNGC	6 cut(s)	1286, 1326, 1626, 1650, 1874, 2354
Sau96I	GGNCC	6 cut(s)	769, 1053, 1261, 1319, 1707, 2422
ScaI	AGTACT	1 cut(s)	1682
SchI	GAGTC	3 cut(s)	187, 2312, 2347
ScrFI	CCNGG	8 cut(s)	222, 516, 615, 827, 1175, 1442, 1583, 2233
SduI	GDGCHC	1 cut(s)	476
SexAI	ACCWGGT	1 cut(s)	1581
SfcI	CTRYAG	2 cut(s)	282, 1919
SinI	GGWCC	3 cut(s)	1261, 1319, 1707
SmiMI	CAYNNNNRTG	3 cut(s)	342, 1233, 1413
SmlI	CTYRAG	4 cut(s)	116, 866, 2064, 2248
SmoI	CTYRAG	4 cut(s)	116, 866, 2064, 2248
SsiI	CCGC	6 cut(s)	445, 642, 1264, 1626, 2294, 2356
SspI	AATATT	1 cut(s)	1946
SspMI	CTAG	1 cut(s)	6
StyD4I	CCNGG	8 cut(s)	220, 514, 613, 825, 1173, 1440, 1581, 2231
StyI	CCWWGG	6 cut(s)	127, 487, 1280, 1289, 1687, 2175
TaaI	ACNGT	5 cut(s)	265, 1102, 1225, 1307, 1718
TaiI	ACGT	1 cut(s)	1384
TaqI	TCGA	3 cut(s)	536, 1030, 2343
TatI	WGTACW	2 cut(s)	1680, 2486
TauI	GCSGC	1 cut(s)	1628
TfiI	GAWTC	3 cut(s)	20, 538, 1526
Tru1I	TTAA	4 cut(s)	867, 996, 1364, 1779
Tru9I	TTAA	4 cut(s)	867, 996, 1364, 1779
TscAI	CASTG	2 cut(s)	1107, 1723
TseFI	GTSAC	2 cut(s)	259, 876
TseI	GCWGC	5 cut(s)	1285, 1325, 1649, 1873, 2353
Tsp45I	GTSAC	2 cut(s)	259, 876
TspDTI	ATGAA	6 cut(s)	476, 1592, 1651, 1914, 2148, 2432
TspGWI	ACGGA	3 cut(s)	243, 965, 2274
TspRI	CASTG	2 cut(s)	1107, 1723
Van91I	CCANNNNNTGG	2 cut(s)	488, 2504
Vha464I	CTTAAG	1 cut(s)	866
VpaK11BI	GGWCC	3 cut(s)	1261, 1319, 1707
XagI	CCTNNNNNAGG	1 cut(s)	2236
XapI	RAATTY	2 cut(s)	1860, 1966
XceI	RCATGY	1 cut(s)	1625
XcmI	CCANNNNNNNNNTGG	3 cut(s)	677, 1287, 1685
XmiI	GTMKAC	1 cut(s)	1618
XmnI	GAANNNNTTC	1 cut(s)	1833
XspI	CTAG	1 cut(s)	6
ZrmI	AGTACT	1 cut(s)	1682
Zsp2I	ATGCAT	1 cut(s)	1420

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.