Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003459

Physical Location & Seq

Genomic Coordinates Reverse (-)

12787937 .. 12791880

Gene Structure 3944 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ23087

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1416 bp

ATGAGCAGACAGTTCAAAACCCTAAAGAGCTCAGAGAATGATCGGAACCGCCAAACCGCCGCAACCTCCGACGCCACCACAATTCCTTACTTACCTGACAACGTCATCGAAAAAATCCTGTCCTTGCTTCCGATCAATCTAGCGGCCCAGGCGAACACCGTCTCGAAGCAATGGGCAGCCCTGTGGCCTTCGTTCCCAGTAATCGATCTCGACGAGGCCGATCCGATCAATTGCGAACTCTATATCGAACCTGGACGTTTCCTCAGCTTCTTGCACTATTCTCTCAGACGATGCAGGGATCAGAGGCTCTTGGACAAGTTGGGGATTCGAGCCAACCTTCGATACGCCGGCGTTTTGGTAGATGAATGGGTTGATTTTGCAATCGAGAGGTGTGTCAAAGAGTTAGATCTTCGAAATTTGGGTGAGAATAGGTACTACCTCCTAGCGCATAGCGTTTTAACTCTGAAATCTTTAACTAGTCTGAATTTGGAGAATGTGAGAGTGTATGCAAGTTCTGGAATTGATGGTCCTGTTAGCCTACCCTTACTGAAAACCATGTCACTCACAGGGTTGGAAGTTTTCGACGATCAGGAGGACAGGGCCATTTGGACCATCCCCAGAATAATTTCGGGGTGTCCTAGCATGGAGCAATTGTCAATTAAGAAATGTAAGCTTGGCTACTCCACCATGGAGCTTGAAATTTCGAGTTACAGTCTCAAATCCTTGGAAATTATGCATTGCCATTCTATGAATTTCGAAGTTACGGCTAAGAATCTCGAGTCTTTTACATTCTATTCGGATTGTACCTGTCCTTACCATAAGACTATGTACTTGTGGGAATGTGATAACTTGAAACATGTGGACGTCTCTTGTGAGGGGTTGAAACAGCTTCTGTTGCATACATGCCTAGAAACTATGGAGAACTCGTTTCACACTCCAAATCTAGAGTCTTTTGAGTTTAGCGGATATCTGAAGGCCAAGGTCCATTTTGTGGATGCTCCATCAAAGTTATTAAAGGCTACAATCAGAGTTTGGGAGACCTCATGGCCCATTGAATATTATTCTGCTATGAGAGATTTTCTTGAAAGTTTCAATTGCTCCAAAAAAGTGAAGCTACATATTGAGGATGCTGAGGGTGTCATAATACCAGAACATCGGAGAATGGAATGGTCTTCCCCATTACCTAGTATTAAGCAGCTGCAGCTATACTTTTATGCTCCATTGGGGGACAGAGAATATTGTTTGAGACCTTCATTGATTTGGATGGCACCATCTGCTGAGATTCTACCATTTCTAGATAATAATGATTATGATTATGATGAGGATGAGGATGATGATGACTATGATGACGACGACGACGACGACAATGATGATGATGACAATGACGACGACGTTGATGACCACAACGAAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

471

Amino Acids

54.39

Weight (kDa)

4.59

Isoelectric Point (pI)

47.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_At1g61320_AtMIF1	PF23622	119 - 268	2.6e-08	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	120 - 273	1.4e-12	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003459)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g05961 FvH4_2g05970 FvH4_2g05980 FvH4_2g05982 FvH4_2g05983 FvH4_2g06070 FvH4_2g06070 FvH4_2g06070 FvH4_2g06070
prunus_persica	Prupe.8G124900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0257151 RchiOBHm_Chr6g0257161 RchiOBHm_Chr6g0257411 RchiOBHm_Chr6g0257421
rosa_laevigata	RLG00000014679 RLG00000014691 RLG00000014692
rosa_multiflora	Rmu_co8284495.1_g000001 Rmu_sc0000820.1_g000043 Rmu_sc0001228.1_g000010 Rmu_sc0001228.1_g000015
rosa_roxburghii	Rroxscaffold_7G00208640
rosa_rugosa	Rorug05G0572700 Rorug05G0573000 Rorug05G0573100 Rorug05G0574400
rosa_samantha	Rh6AG090200 Rh6AG092400 Rh6AG092500 Rh6BG081700 Rh6BG083200 Rh6BG083400 Rh6CG080000 Rh6CG080100
rosa_wichuraiana	Rw6G007880 Rw6G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	867
AccB1I	GGYRCC	1 cut(s)	1267
AccB7I	CCANNNNNTGG	1 cut(s)	991
AciI	CCGC	5 cut(s)	49, 57, 60, 143, 963
AclWI	GGATC	2 cut(s)	215, 306
AcsI	RAATTY	4 cut(s)	415, 484, 699, 751
AcuI	CTGAAG	1 cut(s)	992
AcyI	GRCGYC	2 cut(s)	72, 864
AfaI	GTAC	3 cut(s)	434, 805, 830
AfiI	CCNNNNNNNGG	1 cut(s)	991
AflIII	ACRYGT	1 cut(s)	856
AgsI	TTSAA	7 cut(s)	16, 698, 853, 883, 1055, 1085, 1093
AhlI	ACTAGT	1 cut(s)	476
AjnI	CCWGG	2 cut(s)	147, 250
AluBI	AGCT	8 cut(s)	30, 267, 673, 694, 889, 1114, 1198, 1204
AluI	AGCT	8 cut(s)	30, 267, 673, 694, 889, 1114, 1198, 1204
Alw21I	GWGCWC	1 cut(s)	32
Alw26I	GTCTC	5 cut(s)	166, 719, 871, 1031, 1240
AlwI	GGATC	2 cut(s)	215, 306
AlwNI	CAGNNNCTG	1 cut(s)	892
Ama87I	CYCGRG	1 cut(s)	776
AoxI	GGCC	6 cut(s)	144, 185, 216, 600, 975, 1046
ApeKI	GCWGC	4 cut(s)	176, 1195, 1198, 1201
ApoI	RAATTY	4 cut(s)	415, 484, 699, 751
ArsI	GACNNNNNNTTYG	2 cut(s)	932, 964
Asp700I	GAANNNNTTC	1 cut(s)	625
AspLEI	GCGC	1 cut(s)	448
AspS9I	GGNCC	6 cut(s)	145, 527, 600, 609, 982, 1047
AsuHPI	GGTGA	1 cut(s)	434
AsuII	TTCGAA	2 cut(s)	412, 756
AvaI	CYCGRG	1 cut(s)	776
AvaII	GGWCC	3 cut(s)	527, 609, 982
BanI	GGYRCC	1 cut(s)	1267
BanII	GRGCYC	1 cut(s)	32
BbsI	GAAGAC	1 cut(s)	1164
Bbv12I	GWGCWC	1 cut(s)	32
BbvCI	CCTCAGC	2 cut(s)	263, 1131
BbvI	GCAGC	4 cut(s)	188, 1185, 1207, 1213
BccI	CCATC	5 cut(s)	518, 620, 1009, 1258, 1279
BceAI	ACGGC	1 cut(s)	780
BciT130I	CCWGG	2 cut(s)	149, 252
BcoDI	GTCTC	5 cut(s)	166, 719, 871, 1031, 1240
BcuI	ACTAGT	1 cut(s)	476
BfaI	CTAG	8 cut(s)	140, 443, 477, 639, 908, 944, 1185, 1295
BfmI	CTRYAG	1 cut(s)	1199
BglII	AGATCT	1 cut(s)	406
BisI	GCNGC	6 cut(s)	60, 144, 177, 1196, 1199, 1202
BlsI	GCNGC	6 cut(s)	61, 145, 178, 1197, 1200, 1203
Bme1390I	CCNGG	2 cut(s)	149, 252
Bme18I	GGWCC	3 cut(s)	527, 609, 982
BmeT110I	CYCGRG	1 cut(s)	776
BmgT120I	GGNCC	6 cut(s)	145, 527, 600, 609, 982, 1047
BmiI	GGNNCC	2 cut(s)	47, 1269
BmrFI	CCNGG	2 cut(s)	149, 252
BmrI	ACTGGG	1 cut(s)	191
BmsI	GCATC	3 cut(s)	281, 985, 1117
BmuI	ACTGGG	1 cut(s)	191
BpiI	GAAGAC	1 cut(s)	1164
Bpu10I	CCTNAGC	2 cut(s)	263, 1131
Bpu14I	TTCGAA	2 cut(s)	412, 756
Bsa29I	ATCGAT	1 cut(s)	204
BsaHI	GRCGYC	2 cut(s)	72, 864
BsaI	GGTCTC	2 cut(s)	1031, 1240
BsaJI	CCNNGG	4 cut(s)	147, 687, 723, 978
BsaXI	ACNNNNNCTCC	4 cut(s)	638, 668, 911, 941
Bsc4I	CCNNNNNNNGG	1 cut(s)	991
Bse118I	RCCGGY	1 cut(s)	347
Bse1I	ACTGG	1 cut(s)	197
Bse3DI	GCAATG	2 cut(s)	176, 736
BseBI	CCWGG	2 cut(s)	149, 252
BseCI	ATCGAT	1 cut(s)	204
BseDI	CCNNGG	4 cut(s)	147, 687, 723, 978
BseGI	GGATG	6 cut(s)	612, 1000, 1132, 1269, 1330, 1336
BseLI	CCNNNNNNNGG	1 cut(s)	991
BseMI	GCAATG	2 cut(s)	176, 736
BseMII	CTCAG	5 cut(s)	45, 277, 298, 1122, 1269
BseNI	ACTGG	1 cut(s)	197
BseXI	GCAGC	4 cut(s)	188, 1185, 1207, 1213
BshFI	GGCC	6 cut(s)	146, 187, 218, 602, 977, 1048
BshNI	GGYRCC	1 cut(s)	1267
BshVI	ATCGAT	1 cut(s)	204
BsiHKAI	GWGCWC	1 cut(s)	32
BsiHKCI	CYCGRG	1 cut(s)	776
BsiSI	CCGG	1 cut(s)	348
BslFI	GGGAC	1 cut(s)	1241
BslI	CCNNNNNNNGG	1 cut(s)	991
BsmAI	GTCTC	5 cut(s)	166, 719, 871, 1031, 1240
BsmBI	CGTCTC	2 cut(s)	166, 871
BsmFI	GGGAC	1 cut(s)	1241
BsnI	GGCC	6 cut(s)	146, 187, 218, 602, 977, 1048
Bso31I	GGTCTC	2 cut(s)	1031, 1240
BsoBI	CYCGRG	1 cut(s)	776
Bsp119I	TTCGAA	2 cut(s)	412, 756
Bsp1286I	GDGCHC	1 cut(s)	32
Bsp143I	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
Bsp19I	CCATGG	1 cut(s)	687
BspACI	CCGC	5 cut(s)	49, 57, 60, 143, 963
BspANI	GGCC	6 cut(s)	146, 187, 218, 602, 977, 1048
BspCNI	CTCAG	5 cut(s)	44, 276, 297, 1123, 1270
BspDI	ATCGAT	1 cut(s)	204
BspLI	GGNNCC	2 cut(s)	47, 1269
BspMAI	CTGCAG	1 cut(s)	1203
BspPI	GGATC	2 cut(s)	215, 306
BspT104I	TTCGAA	2 cut(s)	412, 756
BspT107I	GGYRCC	1 cut(s)	1267
BspTNI	GGTCTC	2 cut(s)	1031, 1240
BsrDI	GCAATG	2 cut(s)	176, 736
BsrFI	RCCGGY	1 cut(s)	347
BsrI	ACTGG	1 cut(s)	197
BssAI	RCCGGY	1 cut(s)	347
BssECI	CCNNGG	4 cut(s)	147, 687, 723, 978
BssMI	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
BssNI	GRCGYC	2 cut(s)	72, 864
BssT1I	CCWWGG	3 cut(s)	687, 723, 978
Bst2UI	CCWGG	2 cut(s)	149, 252
Bst4CI	ACNGT	3 cut(s)	12, 160, 713
BstACI	GRCGYC	2 cut(s)	72, 864
BstAPI	GCANNNNNTGC	1 cut(s)	1274
BstBI	TTCGAA	2 cut(s)	412, 756
BstC8I	GCNNGC	1 cut(s)	349
BstDEI	CTNAG	6 cut(s)	31, 263, 284, 768, 1131, 1278
BstDSI	CCRYGG	1 cut(s)	687
BstF5I	GGATG	6 cut(s)	612, 1000, 1132, 1269, 1330, 1336
BstHHI	GCGC	1 cut(s)	448
BstKTI	GATC	8 cut(s)	43, 135, 208, 223, 228, 301, 409, 589
BstMAI	GTCTC	5 cut(s)	166, 719, 871, 1031, 1240
BstMBI	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
BstMWI	GCNNNNNNNGC	4 cut(s)	149, 895, 1201, 1274
BstNI	CCWGG	2 cut(s)	149, 252
BstNSI	RCATGY	2 cut(s)	860, 906
BstSCI	CCNGG	2 cut(s)	147, 250
BstSFI	CTRYAG	1 cut(s)	1199
BstV1I	GCAGC	4 cut(s)	188, 1185, 1207, 1213
BstV2I	GAAGAC	1 cut(s)	1164
BstX2I	RGATCY	1 cut(s)	406
BstYI	RGATCY	1 cut(s)	406
Bsu15I	ATCGAT	1 cut(s)	204
BsuRI	GGCC	6 cut(s)	146, 187, 218, 602, 977, 1048
BsuTUI	ATCGAT	1 cut(s)	204
BtgI	CCRYGG	1 cut(s)	687
BtsCI	GGATG	6 cut(s)	612, 1000, 1132, 1269, 1330, 1336
Cac8I	GCNNGC	1 cut(s)	349
CaiI	CAGNNNCTG	1 cut(s)	892
CfoI	GCGC	1 cut(s)	448
Cfr10I	RCCGGY	1 cut(s)	347
Cfr13I	GGNCC	6 cut(s)	145, 527, 600, 609, 982, 1047
ClaI	ATCGAT	1 cut(s)	204
CseI	GACGC	1 cut(s)	80
Csp6I	GTAC	3 cut(s)	433, 804, 829
CviAII	CATG	6 cut(s)	556, 643, 688, 857, 903, 1044
CviQI	GTAC	3 cut(s)	433, 804, 829
DdeI	CTNAG	6 cut(s)	31, 263, 284, 768, 1131, 1278
DpnI	GATC	8 cut(s)	42, 134, 207, 222, 227, 300, 408, 588
DpnII	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
Ecl136II	GAGCTC	1 cut(s)	30
Eco130I	CCWWGG	3 cut(s)	687, 723, 978
Eco24I	GRGCYC	1 cut(s)	32
Eco31I	GGTCTC	2 cut(s)	1031, 1240
Eco32I	GATATC	1 cut(s)	968
Eco47I	GGWCC	3 cut(s)	527, 609, 982
Eco53kI	GAGCTC	1 cut(s)	30
Eco57I	CTGAAG	1 cut(s)	992
Eco88I	CYCGRG	1 cut(s)	776
EcoICRI	GAGCTC	1 cut(s)	30
EcoRII	CCWGG	2 cut(s)	147, 250
EcoRV	GATATC	1 cut(s)	968
EcoT14I	CCWWGG	3 cut(s)	687, 723, 978
EcoT22I	ATGCAT	1 cut(s)	738
EcoT38I	GRGCYC	1 cut(s)	32
ErhI	CCWWGG	3 cut(s)	687, 723, 978
Esp3I	CGTCTC	2 cut(s)	166, 871
FaeI	CATG	6 cut(s)	559, 646, 691, 860, 906, 1047
FaqI	GGGAC	1 cut(s)	1241
FatI	CATG	6 cut(s)	555, 642, 687, 856, 902, 1043
Fnu4HI	GCNGC	6 cut(s)	60, 144, 177, 1196, 1199, 1202
FokI	GGATG	6 cut(s)	599, 1007, 1139, 1276, 1337, 1343
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	6 cut(s)	60, 144, 177, 1196, 1199, 1202
FspBI	CTAG	8 cut(s)	140, 443, 477, 639, 908, 944, 1185, 1295
GlaI	GCGC	1 cut(s)	447
GluI	GCNGC	6 cut(s)	60, 144, 177, 1196, 1199, 1202
HaeIII	GGCC	6 cut(s)	146, 187, 218, 602, 977, 1048
HapII	CCGG	1 cut(s)	348
HgaI	GACGC	1 cut(s)	80
HhaI	GCGC	1 cut(s)	448
Hin1I	GRCGYC	2 cut(s)	72, 864
Hin1II	CATG	6 cut(s)	559, 646, 691, 860, 906, 1047
Hin6I	GCGC	1 cut(s)	446
HinP1I	GCGC	1 cut(s)	446
HindIII	AAGCTT	1 cut(s)	671
HinfI	GANTC	5 cut(s)	325, 772, 779, 947, 1282
HpaII	CCGG	1 cut(s)	348
HphI	GGTGA	1 cut(s)	434
Hpy166II	GTNNAC	1 cut(s)	862
Hpy188III	TCNNGA	9 cut(s)	163, 209, 385, 516, 590, 776, 944, 1082, 1295
Hpy8I	GTNNAC	1 cut(s)	862
Hpy99I	CGWCG	9 cut(s)	74, 215, 587, 1355, 1358, 1361, 1364, 1391, 1394
HpyAV	CCTTC	4 cut(s)	198, 347, 967, 1260
HpyCH4III	ACNGT	3 cut(s)	12, 160, 713
HpyCH4IV	ACGT	4 cut(s)	102, 256, 864, 1392
HpyCH4V	TGCA	7 cut(s)	274, 294, 380, 509, 736, 898, 1201
HpyF10VI	GCNNNNNNNGC	4 cut(s)	149, 895, 1201, 1274
HpyF3I	CTNAG	6 cut(s)	31, 263, 284, 768, 1131, 1278
HpySE526I	ACGT	4 cut(s)	102, 256, 864, 1392
Hsp92I	GRCGYC	2 cut(s)	72, 864
Hsp92II	CATG	6 cut(s)	559, 646, 691, 860, 906, 1047
HspAI	GCGC	1 cut(s)	446
KroI	GCCGGC	1 cut(s)	347
KroNI	GCCGGC	1 cut(s)	349
Kzo9I	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
LmnI	GCTCC	5 cut(s)	646, 691, 1003, 1103, 1222
Lsp1109I	GCAGC	4 cut(s)	188, 1185, 1207, 1213
LweI	GCATC	3 cut(s)	281, 985, 1117
MaeI	CTAG	8 cut(s)	140, 443, 477, 639, 908, 944, 1185, 1295
MaeII	ACGT	4 cut(s)	102, 256, 864, 1392
MaeIII	GTNAC	3 cut(s)	558, 707, 760
MalI	GATC	8 cut(s)	42, 134, 207, 222, 227, 300, 408, 588
MboI	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
MboII	GAAGA	2 cut(s)	401, 1164
MfeI	CAATTG	3 cut(s)	229, 650, 1093
MflI	RGATCY	1 cut(s)	406
MhlI	GDGCHC	1 cut(s)	32
MlyI	GAGTC	2 cut(s)	788, 956
MmeI	TCCRAC	2 cut(s)	93, 552
Mph1103I	ATGCAT	1 cut(s)	738
MreI	CGCCGGCG	1 cut(s)	347
MroNI	GCCGGC	1 cut(s)	347
MroXI	GAANNNNTTC	1 cut(s)	625
MseI	TTAA	5 cut(s)	458, 473, 660, 1013, 1191
MspA1I	CMGCKG	1 cut(s)	1198
MspI	CCGG	1 cut(s)	348
MspR9I	CCNGG	2 cut(s)	149, 252
MunI	CAATTG	3 cut(s)	229, 650, 1093
MvaI	CCWGG	2 cut(s)	149, 252
MwoI	GCNNNNNNNGC	4 cut(s)	149, 895, 1201, 1274
NaeI	GCCGGC	1 cut(s)	349
NcoI	CCATGG	1 cut(s)	687
NdeII	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
NgoMIV	GCCGGC	1 cut(s)	347
NlaIII	CATG	6 cut(s)	559, 646, 691, 860, 906, 1047
NlaIV	GGNNCC	2 cut(s)	47, 1269
NmuCI	GTSAC	1 cut(s)	558
NsiI	ATGCAT	1 cut(s)	738
NspI	RCATGY	2 cut(s)	860, 906
NspV	TTCGAA	2 cut(s)	412, 756
PaeR7I	CTCGAG	1 cut(s)	776
PciI	ACATGT	1 cut(s)	856
PcsI	WCGNNNNNNNCGW	4 cut(s)	210, 216, 1356, 1359
PdiI	GCCGGC	1 cut(s)	349
PdmI	GAANNNNTTC	1 cut(s)	625
PfeI	GAWTC	3 cut(s)	325, 772, 1282
PflFI	GACNNNGTC	1 cut(s)	101
PflMI	CCANNNNNTGG	1 cut(s)	991
PkrI	GCNGC	6 cut(s)	61, 145, 178, 1197, 1200, 1203
PleI	GAGTC	2 cut(s)	787, 955
PpsI	GAGTC	2 cut(s)	787, 955
PscI	ACATGT	1 cut(s)	856
Psp124BI	GAGCTC	1 cut(s)	32
Psp6I	CCWGG	2 cut(s)	147, 250
PspGI	CCWGG	2 cut(s)	147, 250
PspN4I	GGNNCC	2 cut(s)	47, 1269
PspPI	GGNCC	6 cut(s)	145, 527, 600, 609, 982, 1047
PstI	CTGCAG	1 cut(s)	1203
PstNI	CAGNNNCTG	1 cut(s)	892
PsuI	RGATCY	1 cut(s)	406
PsyI	GACNNNGTC	1 cut(s)	101
PvuII	CAGCTG	1 cut(s)	1198
RsaI	GTAC	3 cut(s)	434, 805, 830
RsaNI	GTAC	3 cut(s)	433, 804, 829
SacI	GAGCTC	1 cut(s)	32
SaqAI	TTAA	5 cut(s)	458, 473, 660, 1013, 1191
SatI	GCNGC	6 cut(s)	60, 144, 177, 1196, 1199, 1202
Sau3AI	GATC	8 cut(s)	40, 132, 205, 220, 225, 298, 406, 586
Sau96I	GGNCC	6 cut(s)	145, 527, 600, 609, 982, 1047
SchI	GAGTC	2 cut(s)	788, 956
ScrFI	CCNGG	2 cut(s)	149, 252
SduI	GDGCHC	1 cut(s)	32
SfaNI	GCATC	3 cut(s)	281, 985, 1117
SfcI	CTRYAG	1 cut(s)	1199
Sfr274I	CTCGAG	1 cut(s)	776
SfuI	TTCGAA	2 cut(s)	412, 756
SgrAI	CRCCGGYG	1 cut(s)	347
SinI	GGWCC	3 cut(s)	527, 609, 982
SlaI	CTCGAG	1 cut(s)	776
SmlI	CTYRAG	1 cut(s)	776
SmoI	CTYRAG	1 cut(s)	776
SpeI	ACTAGT	1 cut(s)	476
SsiI	CCGC	5 cut(s)	49, 57, 60, 143, 963
SspI	AATATT	2 cut(s)	1058, 1238
SspMI	CTAG	8 cut(s)	140, 443, 477, 639, 908, 944, 1185, 1295
SstI	GAGCTC	1 cut(s)	32
StyD4I	CCNGG	2 cut(s)	147, 250
StyI	CCWWGG	3 cut(s)	687, 723, 978
TaaI	ACNGT	3 cut(s)	12, 160, 713
TaiI	ACGT	4 cut(s)	105, 259, 867, 1395
TatI	WGTACW	1 cut(s)	828
TauI	GCSGC	2 cut(s)	62, 146
TfiI	GAWTC	3 cut(s)	325, 772, 1282
Tru1I	TTAA	5 cut(s)	458, 473, 660, 1013, 1191
Tru9I	TTAA	5 cut(s)	458, 473, 660, 1013, 1191
TseFI	GTSAC	1 cut(s)	558
TseI	GCWGC	4 cut(s)	176, 1195, 1198, 1201
Tsp45I	GTSAC	1 cut(s)	558
TspDTI	ATGAA	3 cut(s)	378, 764, 1242
Tth111I	GACNNNGTC	1 cut(s)	101
Van91I	CCANNNNNTGG	1 cut(s)	991
VpaK11BI	GGWCC	3 cut(s)	527, 609, 982
XapI	RAATTY	4 cut(s)	415, 484, 699, 751
XbaI	TCTAGA	2 cut(s)	943, 1294
XceI	RCATGY	2 cut(s)	860, 906
XhoI	CTCGAG	1 cut(s)	776
XmnI	GAANNNNTTC	1 cut(s)	625
XspI	CTAG	8 cut(s)	140, 443, 477, 639, 908, 944, 1185, 1295
ZraI	GACGTC	1 cut(s)	865
Zsp2I	ATGCAT	1 cut(s)	738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.