Rorug05G0573000

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003459
Physical Location & Seq
Reverse (-)
76707095 .. 76721351
14257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0573000.1

Sequence Viewer

Length: 6855 bp
ATGGAGGGGAAAATGATTATTGCGGTAATTGTTGCATGGGTTGGTTACAAGGCGATTAATAAGTGGATATGGACTCCAATGAAGCGGGGTCCAAATCCAATACATAAGGAAGATAATGATCCACCATCTCTTCCTTCATCGTCAGTTGTATCTTTATCATCACCTCCTCCTCCTAAGGGGAAGGAAGATGCAGCATCTCTTCCTTCATCATCAGCTGAAGTATCATCATCATCACCACCTCCTCCTGAGGGGAAGGAAGATGCAGCATCTCTTCCTTCATCATCAGCTGAAGTATCATCATCATCACCACCTCCTCTTCCTGGGCTGGAGTATGATGTGTTTTTGAGTTTCAGAGGTCCTGACACTCGCACGGGTATTGTATCCCATTTACATGCTGAATTGCAATACAGTGGAATTAAAACGTTCATGGATGCGGAAGAGCTTGAAGCAGGGACTGAAATTTCCTCAAATCTACTTACGGCAATCAAAGAATCGAGGTCTGCAATCGTTCTTCTGTCACCAAACTATGCTTCTTCTGCATGGTGTTTGGATGAACTTGCAAACATTGTTCAAGGCTGGGAAACCAGGAACACAATAGTTCTCCCAGTTTTCTATAATGTGGAACCTCGTGACGTCCGACATCAAATGGGGGTTATTAAAGAAGCCTTCACCACACATGAACAAAGGTATAGTGATGAGAAGGTGAAGCGGTGGAAAGATGCTTTAACAAAGGTGGCCAATCTCTCCGGCTTGGAATCAAAAACATATGGGTCTGACAGACAACTTGTCGAAGGCATTGTGCAATGCGTGTGCAATAAAGTACGAGATCTCGAAATTGAGTCTCCCGGAGATTTTGTAGAATTTGAAGCAACAAGAAAAGCCATGGCTAAGGTAATGAAGGCGCTTAAAGATGATGGGCTCACTGCCATTGGAATCTATGGAACGGGAGGCATTGGTAAGACAACAATGGCAAAACATGTTGCTGCCGAAGCCAAAAGAAAAGGGCTTTTTCGTCATGTGGTGATGGCTGTTGTAACACACAAACCTGACTTGGAAAACATTCAAGGCGCATTGGCAGATCAATTGGGCGTCAAATTGGAGAAGGAGACCATTGTTGGAAGAGCTGGTAGATTGAGTAAGGAGATAATGAGTAGAAATAAGATCCTTGTAATCTTGGATGACATATGGGATGTAATAGATTTGTCAAGCGTTGGAATTCCTAGCTACGAGAAGCTGCAAGATTGCAATTCCAAAGTCCTACTCACCACAAGGAGATTGGATGTTTGCAATTCCATGGATAGCCAACCAAACATTGCTCTCAGTTTCTTATCAGAAGAAGATTCTTGGAACTTGTTTGTGAAGAAAACAAGAATGTCTTCTAAAAAGTTGACCATTTTCAATGATGTAGCAAGGATTGTAGCAAGAGAGTGTGCTGGTCTCCCAATCGCGTTGGTAGCAATTGCTAGAGCACTTGGAGATAAAGAAATGAATGAATGGAAAGAAGCAGCTGAACGACTAAAGTCTGCTCAACCTAGCAACCATGATGATGTTGTGTTCAATTGTATAAAGCTAAGCTATGATCAATTGACATCTGAAGATGCGAAGTTATGCTTCTTGCTTTGCTGCCTATTCCCAGAAGACTATGATATCCAAATTGAAGAGTTGATGACGTATGGGATCGGAAAAGGATTGTTCCGTCAGTACAACACATTGCAAGAAGCCAGAACCATTTGCCGTTCTGCGGTGAGGTCCCTTAAATCTTCTAGCTTGCTTTTGGGCACTAAACATGATGGACAAGTAAGGATGCACGATGTCATCAGAGATGTTGCCAGATTAATTTCGTCATCTGAAGATGGCCAATCTGTTTTGGCTGAAGCTGGTCGTCGATTAACGATGTGGCCAAAGATCAAGGCAGACATGGGCTACTGTGCAATCTCATTAATGATGAACGAAATTAGAAAGCTTCCCAAGAAGTTGGTATGTTCGAAACTCAAGATATTGTTATTACAACACAACCCATATGTCAATACGATCCCAGATTCTTTTCTTGAATGTCTGAATGAATTAAGGGTCTTAGATCTTAGCAACACTAGCATTTCTTCGCTGCCCCCATCATTTTCTCTCCTAAACAACCTCCACACTTTGTATTTAGATTCTTGTGAGAAATTTTCGGACATATCCGTCCTCGGAAAACTTGAAAAGCTTGAGATTCTTAGTCTGAGAGAATGTTGCCCTCAGGAAGTTCCAGAAGAAATAGGACATTTGACTAGTCTGAGGATGTTGGATATCAGTGAATCGCAGGTTGTTAAAATTGCGTCTAAAGTGATATCACGTTTAAGTGAATTAGAGGAACTTTACATGCAATGTGATTTTGCGGATTGGAGGAATGAAGTTGAGGAAGCAGGAAAAGAAACTAACGTTGGCTTTGATGAGTTAACTGGCTTGTCATATTTACACATTTTGAAGGTTGGAATACCTGATACAATGGTCTTGCCTACAAATGTTGATGTCCCTCCGAAGTGGCTTTACTTTGATATATGTATCAGCAGAGACTTTGAAGTCCGAGAATCCACTTGGAAGCCACACTCCTCTCATGATCATTCAAGAACCTTGACTCTTAGCACAACAATTCATACCTTACCGGAGTGGTTTATTGATGTGGTGACAGAGAAAGCAGAGAAACTACAGTACAGAAAGTGCAAGGGCTTAGATAACATTCTTGTGGAGTATGACCGTGGGAGATTAAATGAGCTGAGGCATCTTTCTATAATTGGGCCCCATGAGAATTTGAAAGAGGTGATGAATGCAGATTCAAGGGATTCAAACAATGCTGCATTCCCGAAGTTGGAAGAGTTACATCTCCTCGAGGTTGAAAACATCAACAAGCTATGTGTTGGTAAATTACCACCTCGGTCTCTATGCAATCTCAAGTTGTTGGATGTGAGAAAGTGTCATAACTTGGTGAATGCACTTTTACAATCACCTTTGTTGGAGAGACTGCAATATCTGGAAAAACTTGTTTGTGCACATATGCAGGGATTGGAATTTGTATTTGGATCTGGAAAAACTACTACCTGTAAAAAGTTGAAAGAGATGAGATTGTGGCATCTACCAAAACTAATAAAGATATGGAGTGGTCATGCTTCACATGCAATCTTCTTTAATCTTAAACTTTTGGCAGTTAGTGGGTGCAAGAAGCTGAAAAGTCTCTTCACATCCGATGTAGCTCAATGTCTTTCGCAACTGGAAGACCTTTGGGTGCACTCTTGCTTTAGCTTGGACCGAATTGTTGAAGAGAGCAAGGAAACAGGGAAGAGGAATGTCATTTTTCCAAAATTGAAGAACTTAGTCTTGGAGGATCTTCCACGGCTCAGAAGGTTATACAGTAACAGTAGTGGAGGTCCGACTTCATTGGACATAAAGTTCCCCTCATTGGAACACTTCCACACGAGGGAATGCCCCCGGTTCTCACCCTCTGCGTTTAAATTCCACAGCCAGAAGCAAGTCCATGTCAATGATTTACAACAATATAGCTTTCTAAAGAAAAGGTTGTGGGAGAACATTGAACTTGTGTCTACAATTTCTAAAGATGGGCGGCGTCAGTGGCAGTATGATGTATTTTTGACTTTTATGGGTGAAGACACTCCAGAGGGTTTTACATATGAATTATATGATCGACTGCAAAACAAGAGAGGAATCAATACATTCATGTATGACCAAGACCTTAAAGAAGGAAATGTTATTTCTCCTAGTGTCCTAAACGCAATCGAAGAATCAAAGCTGGCAATTGTTATTTTTTCTCCACTCTATGCTTCTTCTATTTGGTGTTTGGAGGAACTTAGAAACGTTTGTGAATGCATGGAAGACAACAGAGTTCGGCCATTTTTTTACAAGGTCGATCCTACTGATGTACGATATCAGAAGAGGAGTTTTGAAGATGCTTTCACCACACATGAAAACTCTAGGCGACACGGAGACAAGGTGGATACATGGAGAGATGTTTTAAACCATGTAACCAATCTCTCTGGGTGGCATACAAAGAATTACAAGACTGAGAGAGAACTTGTGGACGCAATTGTGGATTCAGTGAGCAGTAAAGTACAACCTATTGAAATTGAGTTTGCATTGTCCACCGGAGATTTTGAAGCATTTGAATCAAGAAGAAAAGCCATGAATGAGGTCGTGGAAGCGCTTACAGATGATGAGGTCACTGCCGTTGGCGTCTGCGGAATGGGAGGCGTCGGCAAGACAACCATGGCAATACATGTCGCTGCACAAGCCTGCATAAATGGGACTTTTCATCATGTGATTATGGCTGTTTTATCCCAAATCTCCGACTTGAGAAAAATTCAAGGCACATTGGCAGATATGTTGGGCGTTAAATTGGAGGAAGAGTCAGAATTTGGAAGAGCTGCTAGATTGAATGAGGAGATAATGAGAAGAGAAAAGATCCTTATAATCTTGGATGACATGTGGGAGAGAATAGATTTGCCAAGCATAGGTATTCCAAGCCAATACGAGCTACAAAATTGCAACATCAAAGTCCTACTCACCACAAGGAGTTTGACTGTCTGTCATGTCATGAGATGCCAAGCAATTATTCAACTAGAGATGTTATCAGAAGAAGATTCTTGGTCCTTGTTTGTGAAAACAGCAAGAAGGGAATTTGAATCGACCCTTTTCCATAATGTAGCGAGGAAGGTGGCTAGAGAATGTTATGGTCTACCAATTGCATTGGTAGCAATTGCAAGGGCACTCGGAGATAAAGATCTGGCGGAATGGCAAAACGCAGCTCAACGACTAGAGAAGCGGCAAATTGACGACATGCACAATTGGGGAGCTGCCGCCATATGCATAAAATCAAGCTATGATTACTTGAAAGATGAAGATTCCAAGTCATGCTTCTTGCTCTGTTGCCTATTTCCAGAAGACTATGACATTCCAATAGAAGACTTGTTCAGGTATGCAATCGGGAAAGGATTATTTCAAGATTCTGACAGAATCAAAGAAGCCAGAGTAACAGCAGATAAAGTGGTCAAGTACCTGAAAGATTCTAACCTGCTTATCGATAGTGTTAAACATGGATGTGTAAGGATGCATGATATCATCCGGGCTACAGGCATGATGATTGCAAGATCTGAAGATGGGCATGGGTTTTGGGTAGAAGCAGGCTGTGGTTTACAGGATTGGCCACGTGAATTACCTAAAGGCTACTCTGCTGTCTCACTAATGGAGAATGATATTCGCAAGCTACCCGAAGAGTTGGTATGCCCAAAACTCCAGATTCTATTACTACAGGGGAATAGTCATCTAGAAGAGATTCCAGAAGCTTTTTTCCAAAGTAGTAATGCATTGAGGGTTTTAGATCTTAGCCACACTAGTATTTCTGCGCTAAGCCTATCACTCAGTCTTCAGTCAGACCTCCGAGGTTTGTATTTGGATTTTTGCCAAAAGATACTTGACATATCCATACTCAAAAGACTTAAGAAGCTTGAGATTCTTAGTATGAGATATACTTGTTTGGAGGAATTGCCGAGAGAAATAGGACATTTAACCAATCTAAGGATGTTGGATGTCACTGGTGGATTGGTTAATACAGCTCCATCAAATGTTATATCAAGGTTGCATAGATTAGAAGAACTGCACATGCAATGTGAGTTTTTTGACTGGGGGAGTAAATTTGATGAGTTAACTGGCTTATTATATTTAAGCATATTGAAGGTTTGCATATCTGATGCAAAATGCTTGCCTGAAACTGTTGAGTTTGAGCCGAATTGGGTATACTTTGACATATGTATCAGCAGAATCCCTCGAGCAAGAGAATCCTCTTGGGATGACCCAGACTCTTCACATGTTCTTTCAAGAGCCTTGACTCTTGACACAACCATCAATACTTTACCGGACTGGTTTATCAGATCAGTGACGCAGAGAACAGAGAAACTACAATACCTAAACTGCAAGGGCTTAAATAATATCCTTGATGAATATGGTCATGGAAGATTACACTGCCTGAAGTATCTCTCTGTAATCGATTGTCATGAGACGTTGAAACAGTTGATGAACCCAAAAGCATGGGATCCAAATGAACCTGTGTTCGAAAATTTGGAAATGATGCATCTCGATATTGTGGGTTGTCTGGAGGAGTTATGTGTTGGTGAATTACCACCTGGATCTCTATCCAACTTAAAGTCATTGAAGGTGGAAGCTTGTCATAACTTGGTGAATGCAGTTTTATCTTCAAATTTGTTGCAGAGACTACATAATCTGGACAAATTAGTTTGTTCACAAATGGATGGAATGAAATATGTGTTTGGATGCGAAGGGCTTGAGCCAGGAAAAATTACCTTGACAAAATTGAGAGAGATGAAGTTGATGATGCTGGAGAATCTTGCAAGTATCGGGAATGGTCCAAGTGTTCCTCGACATTCATTACTCTTCCATAATCTTAAAATTCTGGTAGTTGTCAAGTGCCCTAAGCTAAGATATCTCTTCACATCTGATGTAGCTCGATCTCTTTTGCAATTGGAAGATCTTTGGGTACAGAGTTGTGATAGATTGGAAAGAGTTATTGAAGCAACTGAGGACACAGTGAACTACAACCACAAGATCATCGTTTTTCCAGAATTGAGGAACTTGGTTTTGGAAGATCTTCCAGATCTCACAGGTTTCTACAACATTACTACTGGAAGTGCTACTTCATTGGATGTCGAATGCCCTTCGTTGCAACACATGTATGTGAACAAGTGCCCCCATTTTTCACCCTCTGCTTCTGACTTTCATAGCAGGAACCAAGTACAAGTCAATGATGACCAACATTATCGGTCTCTAACTAAACGGTTTATTTCTGTGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

2284

Amino Acids

258.67

Weight (kDa)

5.91

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 111 - 276 5e-49 TIR domain
TIR_2 PF13676 113 - 209 1.1e-15 TIR domain
NB-ARC PF00931 293 - 459 1.1e-26 NB-ARC domain
LRR_14 PF23598 663 - 789 3.3e-14 Leucine-rich repeat region
LRR_8 PF13855 663 - 720 1e-06 Leucine rich repeat
LRR_4 PF12799 689 - 729 3.6e-06 Leucine Rich repeats (2 copies)
LRR_RPS2 PF23247 949 - 1090 2.7e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
TIR PF01582 1203 - 1369 1.8e-40 TIR domain
TIR_2 PF13676 1206 - 1299 9.5e-09 TIR domain
NB-ARC PF00931 1389 - 1552 9.2e-30 NB-ARC domain
LRR_14 PF23598 1787 - 1909 2.7e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 2046 - 2184 6.5e-27 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4457
AasI GACNNNNNNGTC 2 cut(s) 2180, 2558
AatII GACGTC 1 cut(s) 636
AbsI CCTCGAGG 1 cut(s) 2864
Acc36I ACCTGC 2 cut(s) 2290, 5067
AccI GTMKAC 3 cut(s) 3575, 4723, 5781
AccII CGCG 1 cut(s) 1448
AclI AACGTT 3 cut(s) 422, 2418, 3846
AcoI YGGCCR 5 cut(s) 735, 1855, 1898, 3878, 5189
AcuI CTGAAG 8 cut(s) 237, 309, 1614, 1869, 1893, 5160, 5396, 6032
AcvI CACGTG 1 cut(s) 5195
AcyI GRCGYC 5 cut(s) 633, 1089, 3598, 4221, 4239
AfaI GTAC 8 cut(s) 822, 1703, 2690, 3912, 4101, 5042, 6540, 6795
AfeI AGCGCT 1 cut(s) 4191
AfiI CCNNNNNNNGG 9 cut(s) 176, 248, 320, 1741, 2845, 3433, 3451, 4499, 5562
AflII CTTAAG 1 cut(s) 5483
AflIII ACRYGT 5 cut(s) 976, 4264, 4470, 5851, 6729
AhdI GACNNNNNGTC 2 cut(s) 785, 4572
AhlI ACTAGT 2 cut(s) 2267, 5378
AjnI CCWGG 4 cut(s) 319, 584, 6166, 6331
AloI GAACNNNNNNTCC 2 cut(s) 3407, 3439
Alw21I GWGCWC 3 cut(s) 1471, 3028, 3264
Alw44I GTGCAC 2 cut(s) 3024, 3260
AlwNI CAGNNNCTG 1 cut(s) 455
Ama87I CYCGRG 2 cut(s) 2864, 5811
Aor51HI AGCGCT 1 cut(s) 4191
AoxI GGCC 6 cut(s) 735, 1855, 1898, 2774, 3878, 5189
ApaI GGGCCC 1 cut(s) 2778
ApaLI GTGCAC 2 cut(s) 3024, 3260
AseI ATTAAT 3 cut(s) 57, 1835, 1940
Asp700I GAANNNNTTC 4 cut(s) 1059, 1375, 5319, 6648
AspLEI GCGC 4 cut(s) 904, 1070, 4192, 5392
AspS9I GGNCC 9 cut(s) 89, 356, 1749, 2774, 2775, 3280, 3401, 4635, 6407
AsuC2I CCSGG 3 cut(s) 846, 3463, 5111
AsuII TTCGAA 2 cut(s) 1985, 6096
AvaI CYCGRG 2 cut(s) 2864, 5811
AvaII GGWCC 7 cut(s) 89, 356, 1749, 3280, 3401, 4635, 6407
AxyI CCTNAGG 3 cut(s) 174, 246, 2235
BaeGI GKGCMC 7 cut(s) 1781, 2778, 3028, 3264, 4756, 6473, 6749
BaeI ACNNNNGTAYC 4 cut(s) 2472, 2505, 5779, 5812
BalI TGGCCA 4 cut(s) 737, 1857, 1900, 5191
BamHI GGATCC 1 cut(s) 6076
BanII GRGCYC 2 cut(s) 921, 2778
BarI GAAGNNNNNNTAC 2 cut(s) 2510, 2542
BauI CACGAG 2 cut(s) 627, 3448
BbrPI CACGTG 1 cut(s) 5195
BbsI GAAGAC 8 cut(s) 1368, 1644, 3255, 3645, 3870, 4935, 4956, 5402
Bbv12I GWGCWC 3 cut(s) 1471, 3028, 3264
BbvCI CCTCAGC 1 cut(s) 2753
BceAI ACGGC 4 cut(s) 495, 1719, 3383, 4199
BciT130I CCWGG 4 cut(s) 321, 586, 6168, 6333
BciVI GTATCC 2 cut(s) 391, 3979
BclI TGATCA 2 cut(s) 1579, 2596
BcnI CCSGG 3 cut(s) 846, 3463, 5111
BcuI ACTAGT 2 cut(s) 2267, 5378
BfmI CTRYAG 3 cut(s) 2684, 5115, 5294
BfoI RGCGCY 2 cut(s) 905, 4193
BfrI CTTAAG 1 cut(s) 5483
BfuAI ACCTGC 2 cut(s) 2290, 5067
BfuI GTATCC 2 cut(s) 391, 3979
BglII AGATCT 8 cut(s) 826, 2077, 4768, 5135, 5365, 6529, 6646, 6655
BlpI GCTNAGC 2 cut(s) 1571, 5393
Bme1390I CCNGG 7 cut(s) 321, 586, 846, 3463, 5111, 6168, 6333
Bme18I GGWCC 7 cut(s) 89, 356, 1749, 3280, 3401, 4635, 6407
BmeRI GACNNNNNGTC 2 cut(s) 785, 4572
BmeT110I CYCGRG 2 cut(s) 2864, 5811
BmgT120I GGNCC 9 cut(s) 89, 356, 1749, 2774, 2775, 3280, 3401, 4635, 6407
BmiI GGNNCC 7 cut(s) 90, 624, 1751, 2776, 2777, 6078, 6788
BmrFI CCNGG 7 cut(s) 321, 586, 846, 3463, 5111, 6168, 6333
BmrI ACTGGG 2 cut(s) 599, 5677
BmuI ACTGGG 2 cut(s) 599, 5677
BoxI GACNNNNGTC 1 cut(s) 1519
BpiI GAAGAC 8 cut(s) 1368, 1644, 3255, 3645, 3870, 4935, 4956, 5402
BpmI CTGGAG 5 cut(s) 347, 3630, 5264, 6158, 6401
Bpu10I CCTNAGC 3 cut(s) 888, 2753, 6474
Bpu1102I GCTNAGC 2 cut(s) 1571, 5393
Bpu14I TTCGAA 2 cut(s) 1985, 6096
BpuEI CTTGAG 6 cut(s) 1976, 2225, 2912, 4360, 5513, 6347
BpuMI CCSGG 3 cut(s) 846, 3463, 5111
Bsa29I ATCGAT 2 cut(s) 5067, 6030
BsaAI YACGTR 1 cut(s) 5195
BsaBI GATNNNNATC 3 cut(s) 117, 4767, 6175
BsaHI GRCGYC 5 cut(s) 633, 1089, 3598, 4221, 4239
BsaI GGTCTC 4 cut(s) 1100, 1442, 2919, 6828
BsaWI WCCGGW 3 cut(s) 2641, 4133, 5899
BsaXI ACNNNNNCTCC 6 cut(s) 840, 870, 3966, 3996, 4379, 4409
Bsc4I CCNNNNNNNGG 9 cut(s) 176, 248, 320, 1741, 2845, 3433, 3451, 4499, 5562
Bse1I ACTGG 8 cut(s) 605, 2443, 3249, 5584, 5672, 5698, 5909, 6688
Bse21I CCTNAGG 3 cut(s) 174, 246, 2235
Bse3DI GCAATG 5 cut(s) 809, 1311, 1709, 2369, 5657
Bse8I GATNNNNATC 3 cut(s) 117, 4767, 6175
BseBI CCWGG 4 cut(s) 321, 586, 6168, 6333
BseCI ATCGAT 2 cut(s) 5067, 6030
BseJI GATNNNNATC 3 cut(s) 117, 4767, 6175
BseLI CCNNNNNNNGG 9 cut(s) 176, 248, 320, 1741, 2845, 3433, 3451, 4499, 5562
BseMI GCAATG 5 cut(s) 809, 1311, 1709, 2369, 5657
BseNI ACTGG 8 cut(s) 605, 2443, 3249, 5584, 5672, 5698, 5909, 6688
BseRI GAGGAG 9 cut(s) 156, 159, 231, 303, 2578, 2852, 3940, 4442, 6155
BseSI GKGCMC 7 cut(s) 1781, 2778, 3028, 3264, 4756, 6473, 6749
BseYI CCCAGC 1 cut(s) 576
BsgI GTGCAG 2 cut(s) 4257, 5627
Bsh1236I CGCG 1 cut(s) 1448
BshFI GGCC 6 cut(s) 737, 1857, 1900, 2776, 3880, 5191
BshVI ATCGAT 2 cut(s) 5067, 6030
BsiHKAI GWGCWC 3 cut(s) 1471, 3028, 3264
BsiHKCI CYCGRG 2 cut(s) 2864, 5811
BsiSI CCGG 7 cut(s) 747, 846, 2642, 3463, 4134, 5110, 5900
BslFI GGGAC 4 cut(s) 466, 1735, 2495, 4306
BslI CCNNNNNNNGG 9 cut(s) 176, 248, 320, 1741, 2845, 3433, 3451, 4499, 5562
BsmBI CGTCTC 1 cut(s) 6035
BsmFI GGGAC 4 cut(s) 466, 1735, 2495, 4306
BsmI GAATGC 7 cut(s) 2809, 2834, 2971, 3461, 3860, 6229, 6716
BsnI GGCC 6 cut(s) 737, 1857, 1900, 2776, 3880, 5191
Bso31I GGTCTC 4 cut(s) 1100, 1442, 2919, 6828
BsoBI CYCGRG 2 cut(s) 2864, 5811
Bsp119I TTCGAA 2 cut(s) 1985, 6096
Bsp120I GGGCCC 1 cut(s) 2774
Bsp1286I GDGCHC 9 cut(s) 921, 1471, 1781, 2778, 3028, 3264, 4756, 6473, 6749
Bsp1720I GCTNAGC 2 cut(s) 1571, 5393
Bsp19I CCATGG 3 cut(s) 882, 1293, 4254
BspANI GGCC 6 cut(s) 737, 1857, 1900, 2776, 3880, 5191
BspDI ATCGAT 2 cut(s) 5067, 6030
BspFNI CGCG 1 cut(s) 1448
BspHI TCATGA 3 cut(s) 2593, 4581, 6037
BspLI GGNNCC 7 cut(s) 90, 624, 1751, 2776, 2777, 6078, 6788
BspMI ACCTGC 2 cut(s) 2290, 5067
BspQI GCTCTTC 3 cut(s) 432, 1114, 4402
BspT104I TTCGAA 2 cut(s) 1985, 6096
BspTI CTTAAG 1 cut(s) 5483
BspTNI GGTCTC 4 cut(s) 1100, 1442, 2919, 6828
BsrDI GCAATG 5 cut(s) 809, 1311, 1709, 2369, 5657
BsrI ACTGG 8 cut(s) 605, 2443, 3249, 5584, 5672, 5698, 5909, 6688
BssNAI GTATAC 1 cut(s) 5782
BssNI GRCGYC 5 cut(s) 633, 1089, 3598, 4221, 4239
BssSI CACGAG 2 cut(s) 627, 3448
BssT1I CCWWGG 3 cut(s) 882, 1293, 4254
Bst1107I GTATAC 1 cut(s) 5782
Bst2BI CACGAG 2 cut(s) 627, 3448
Bst2UI CCWGG 4 cut(s) 321, 586, 6168, 6333
BstACI GRCGYC 5 cut(s) 633, 1089, 3598, 4221, 4239
BstAFI CTTAAG 1 cut(s) 5483
BstBAI YACGTR 1 cut(s) 5195
BstBI TTCGAA 2 cut(s) 1985, 6096
BstC8I GCNNGC 6 cut(s) 1769, 3783, 4282, 5170, 5249, 5747
BstDSI CCRYGG 5 cut(s) 882, 1293, 2734, 3365, 4254
BstFNI CGCG 1 cut(s) 1448
BstH2I RGCGCY 2 cut(s) 905, 4193
BstHHI GCGC 4 cut(s) 904, 1070, 4192, 5392
BstMWI GCNNNNNNNGC 8 cut(s) 536, 989, 1454, 2091, 3149, 3604, 4277, 4739
BstNI CCWGG 4 cut(s) 321, 586, 6168, 6333
BstPAI GACNNNNGTC 1 cut(s) 1519
BstSCI CCNGG 7 cut(s) 319, 584, 844, 3461, 5109, 6166, 6331
BstSFI CTRYAG 3 cut(s) 2684, 5115, 5294
BstSLI GKGCMC 7 cut(s) 1781, 2778, 3028, 3264, 4756, 6473, 6749
BstUI CGCG 1 cut(s) 1448
BstV2I GAAGAC 8 cut(s) 1368, 1644, 3255, 3645, 3870, 4935, 4956, 5402
BstXI CCANNNNNNTGG 2 cut(s) 1975, 6072
BstZ17I GTATAC 1 cut(s) 5782
Bsu15I ATCGAT 2 cut(s) 5067, 6030
Bsu36I CCTNAGG 3 cut(s) 174, 246, 2235
BsuI GTATCC 2 cut(s) 391, 3979
BsuRI GGCC 6 cut(s) 737, 1857, 1900, 2776, 3880, 5191
BsuTUI ATCGAT 2 cut(s) 5067, 6030
BtgI CCRYGG 5 cut(s) 882, 1293, 2734, 3365, 4254
BtsI GCAGTG 3 cut(s) 921, 4209, 6004
BveI ACCTGC 2 cut(s) 2290, 5067
Cac8I GCNNGC 6 cut(s) 1769, 3783, 4282, 5170, 5249, 5747
CaiI CAGNNNCTG 1 cut(s) 455
CciI TCATGA 3 cut(s) 2593, 4581, 6037
CfoI GCGC 4 cut(s) 904, 1070, 4192, 5392
Cfr13I GGNCC 9 cut(s) 89, 356, 1749, 2774, 2775, 3280, 3401, 4635, 6407
ClaI ATCGAT 2 cut(s) 5067, 6030
CseI GACGC 7 cut(s) 1078, 2304, 3587, 4079, 4210, 4228, 5932
Csp6I GTAC 8 cut(s) 821, 1702, 2689, 3911, 4100, 5041, 6539, 6794
CviQI GTAC 8 cut(s) 821, 1702, 2689, 3911, 4100, 5041, 6539, 6794
DraI TTTAAA 2 cut(s) 3484, 4005
DrdI GACNNNNNNGTC 2 cut(s) 2180, 2558
DriI GACNNNNNGTC 2 cut(s) 785, 4572
DseDI GACNNNNNNGTC 2 cut(s) 2180, 2558
EaeI YGGCCR 5 cut(s) 735, 1855, 1898, 3878, 5189
Eam1105I GACNNNNNGTC 2 cut(s) 785, 4572
EciI GGCGGA 1 cut(s) 4790
Eco130I CCWWGG 3 cut(s) 882, 1293, 4254
Eco24I GRGCYC 2 cut(s) 921, 2778
Eco31I GGTCTC 4 cut(s) 1100, 1442, 2919, 6828
Eco32I GATATC 6 cut(s) 1648, 2287, 2328, 3917, 5104, 6485
Eco47I GGWCC 7 cut(s) 89, 356, 1749, 3280, 3401, 4635, 6407
Eco47III AGCGCT 1 cut(s) 4191
Eco57I CTGAAG 8 cut(s) 237, 309, 1614, 1869, 1893, 5160, 5396, 6032
Eco72I CACGTG 1 cut(s) 5195
Eco81I CCTNAGG 3 cut(s) 174, 246, 2235
Eco88I CYCGRG 2 cut(s) 2864, 5811
EcoO109I RGGNCCY 3 cut(s) 356, 1749, 2775
EcoRI GAATTC 1 cut(s) 1215
EcoRII CCWGG 4 cut(s) 319, 584, 6166, 6331
EcoRV GATATC 6 cut(s) 1648, 2287, 2328, 3917, 5104, 6485
EcoT14I CCWWGG 3 cut(s) 882, 1293, 4254
EcoT22I ATGCAT 5 cut(s) 3860, 4856, 5100, 5353, 6117
EcoT38I GRGCYC 2 cut(s) 921, 2778
ErhI CCWWGG 3 cut(s) 882, 1293, 4254
Esp3I CGTCTC 1 cut(s) 6035
FalI AAGNNNNNCTT 8 cut(s) 1360, 1392, 1595, 1627, 4886, 4918, 6679, 6711
FaqI GGGAC 4 cut(s) 466, 1735, 2495, 4306
FauI CCCGC 1 cut(s) 78
FauNDI CATATG 7 cut(s) 766, 1184, 2020, 3030, 3661, 4850, 5792
FbaI TGATCA 2 cut(s) 1579, 2596
FblI GTMKAC 3 cut(s) 3575, 4723, 5781
FriOI GRGCYC 2 cut(s) 921, 2778
GlaI GCGC 4 cut(s) 903, 1069, 4191, 5391
GsaI CCCAGC 1 cut(s) 580
GsuI CTGGAG 5 cut(s) 347, 3630, 5264, 6158, 6401
HaeII RGCGCY 2 cut(s) 905, 4193
HaeIII GGCC 6 cut(s) 737, 1857, 1900, 2776, 3880, 5191
HapII CCGG 7 cut(s) 747, 846, 2642, 3463, 4134, 5110, 5900
HgaI GACGC 7 cut(s) 1078, 2304, 3587, 4079, 4210, 4228, 5932
HhaI GCGC 4 cut(s) 904, 1070, 4192, 5392
Hin1I GRCGYC 5 cut(s) 633, 1089, 3598, 4221, 4239
Hin6I GCGC 4 cut(s) 902, 1068, 4190, 5390
HinP1I GCGC 4 cut(s) 902, 1068, 4190, 5390
HincII GTYRAC 3 cut(s) 1389, 2436, 5691
HindII GTYRAC 3 cut(s) 1389, 2436, 5691
HindIII AAGCTT 5 cut(s) 1961, 2201, 5328, 5489, 6204
HpaI GTTAAC 2 cut(s) 2436, 5691
HpaII CCGG 7 cut(s) 747, 846, 2642, 3463, 4134, 5110, 5900
Hpy99I CGWCG 2 cut(s) 1887, 4244
HpyCH4IV ACGT 8 cut(s) 422, 633, 1670, 2332, 2418, 3846, 5194, 6044
HpyF10VI GCNNNNNNNGC 8 cut(s) 536, 989, 1454, 2091, 3149, 3604, 4277, 4739
HpySE526I ACGT 8 cut(s) 422, 633, 1670, 2332, 2418, 3846, 5194, 6044
Hsp92I GRCGYC 5 cut(s) 633, 1089, 3598, 4221, 4239
HspAI GCGC 4 cut(s) 902, 1068, 4190, 5390
Ksp22I TGATCA 2 cut(s) 1579, 2596
KspAI GTTAAC 2 cut(s) 2436, 5691
LguI GCTCTTC 3 cut(s) 432, 1114, 4402
LmnI GCTCC 2 cut(s) 4838, 5605
MaeII ACGT 8 cut(s) 422, 633, 1670, 2332, 2418, 3846, 5194, 6044
MhlI GDGCHC 9 cut(s) 921, 1471, 1781, 2778, 3028, 3264, 4756, 6473, 6749
MlsI TGGCCA 4 cut(s) 737, 1857, 1900, 5191
MluNI TGGCCA 4 cut(s) 737, 1857, 1900, 5191
MlyI GAGTC 6 cut(s) 67, 848, 2608, 4403, 5837, 5866
Mox20I TGGCCA 4 cut(s) 737, 1857, 1900, 5191
Mph1103I ATGCAT 5 cut(s) 3860, 4856, 5100, 5353, 6117
MroXI GAANNNNTTC 4 cut(s) 1059, 1375, 5319, 6648
MscI TGGCCA 4 cut(s) 737, 1857, 1900, 5191
MslI CAYNNNNRTG 7 cut(s) 390, 1545, 2720, 3513, 5085, 6732, 6734
Msp20I TGGCCA 4 cut(s) 737, 1857, 1900, 5191
MspA1I CMGCKG 3 cut(s) 215, 287, 1508
MspCI CTTAAG 1 cut(s) 5483
MspI CCGG 7 cut(s) 747, 846, 2642, 3463, 4134, 5110, 5900
MspR9I CCNGG 7 cut(s) 321, 586, 846, 3463, 5111, 6168, 6333
Mva1269I GAATGC 7 cut(s) 2809, 2834, 2971, 3461, 3860, 6229, 6716
MvaI CCWGG 4 cut(s) 321, 586, 6168, 6333
MvnI CGCG 1 cut(s) 1448
MwoI GCNNNNNNNGC 8 cut(s) 536, 989, 1454, 2091, 3149, 3604, 4277, 4739
NciI CCSGG 3 cut(s) 846, 3463, 5111
NcoI CCATGG 3 cut(s) 882, 1293, 4254
NdeI CATATG 7 cut(s) 766, 1184, 2020, 3030, 3661, 4850, 5792
NlaIV GGNNCC 7 cut(s) 90, 624, 1751, 2776, 2777, 6078, 6788
NmeAIII GCCGAG 1 cut(s) 5559
NmuCI GTSAC 6 cut(s) 516, 629, 2662, 4207, 5575, 5920
NsiI ATGCAT 5 cut(s) 3860, 4856, 5100, 5353, 6117
NspV TTCGAA 2 cut(s) 1985, 6096
PaeR7I CTCGAG 2 cut(s) 2864, 5811
PagI TCATGA 3 cut(s) 2593, 4581, 6037
PciI ACATGT 5 cut(s) 976, 4264, 4470, 5851, 6729
PciSI GCTCTTC 3 cut(s) 432, 1114, 4402
PcsI WCGNNNNNNNCGW 1 cut(s) 634
PctI GAATGC 7 cut(s) 2809, 2834, 2971, 3461, 3860, 6229, 6716
PdmI GAANNNNTTC 4 cut(s) 1059, 1375, 5319, 6648
PfoI TCCNGGA 1 cut(s) 844
PleI GAGTC 6 cut(s) 67, 847, 2608, 4402, 5837, 5866
PmaCI CACGTG 1 cut(s) 5195
PmlI CACGTG 1 cut(s) 5195
PpsI GAGTC 6 cut(s) 67, 847, 2608, 4402, 5837, 5866
Ppu21I YACGTR 1 cut(s) 5195
PpuMI RGGWCCY 2 cut(s) 356, 1749
PscI ACATGT 5 cut(s) 976, 4264, 4470, 5851, 6729
PshAI GACNNNNGTC 1 cut(s) 1519
PshBI ATTAAT 3 cut(s) 57, 1835, 1940
PsiI TTATAA 1 cut(s) 4457
Psp1406I AACGTT 3 cut(s) 422, 2418, 3846
Psp5II RGGWCCY 2 cut(s) 356, 1749
Psp6I CCWGG 4 cut(s) 319, 584, 6166, 6331
PspCI CACGTG 1 cut(s) 5195
PspFI CCCAGC 1 cut(s) 576
PspGI CCWGG 4 cut(s) 319, 584, 6166, 6331
PspN4I GGNNCC 7 cut(s) 90, 624, 1751, 2776, 2777, 6078, 6788
PspOMI GGGCCC 1 cut(s) 2774
PspPI GGNCC 9 cut(s) 89, 356, 1749, 2774, 2775, 3280, 3401, 4635, 6407
PspPPI RGGWCCY 2 cut(s) 356, 1749
PspXI VCTCGAGB 2 cut(s) 2864, 5811
PstNI CAGNNNCTG 1 cut(s) 455
PvuII CAGCTG 3 cut(s) 215, 287, 1508
RsaI GTAC 8 cut(s) 822, 1703, 2690, 3912, 4101, 5042, 6540, 6795
RsaNI GTAC 8 cut(s) 821, 1702, 2689, 3911, 4100, 5041, 6539, 6794
RseI CAYNNNNRTG 7 cut(s) 390, 1545, 2720, 3513, 5085, 6732, 6734
SapI GCTCTTC 3 cut(s) 432, 1114, 4402
Sau96I GGNCC 9 cut(s) 89, 356, 1749, 2774, 2775, 3280, 3401, 4635, 6407
SchI GAGTC 6 cut(s) 67, 848, 2608, 4403, 5837, 5866
ScrFI CCNGG 7 cut(s) 321, 586, 846, 3463, 5111, 6168, 6333
SduI GDGCHC 9 cut(s) 921, 1471, 1781, 2778, 3028, 3264, 4756, 6473, 6749
SfcI CTRYAG 3 cut(s) 2684, 5115, 5294
Sfr274I CTCGAG 2 cut(s) 2864, 5811
SfuI TTCGAA 2 cut(s) 1985, 6096
SinI GGWCC 7 cut(s) 89, 356, 1749, 3280, 3401, 4635, 6407
SlaI CTCGAG 2 cut(s) 2864, 5811
SmiMI CAYNNNNRTG 7 cut(s) 390, 1545, 2720, 3513, 5085, 6732, 6734
SmlI CTYRAG 9 cut(s) 1991, 2204, 2864, 2927, 4339, 5483, 5492, 5811, 6326
SmoI CTYRAG 9 cut(s) 1991, 2204, 2864, 2927, 4339, 5483, 5492, 5811, 6326
SpeI ACTAGT 2 cut(s) 2267, 5378
StyD4I CCNGG 7 cut(s) 319, 584, 844, 3461, 5109, 6166, 6331
StyI CCWWGG 3 cut(s) 882, 1293, 4254
TaiI ACGT 8 cut(s) 425, 636, 1673, 2335, 2421, 3849, 5197, 6047
TaqII GACCGA 3 cut(s) 2901, 3297, 6810
TatI WGTACW 4 cut(s) 1701, 2688, 4099, 6793
TauI GCSGC 3 cut(s) 3598, 4813, 4847
TseFI GTSAC 6 cut(s) 516, 629, 2662, 4207, 5575, 5920
Tsp45I GTSAC 6 cut(s) 516, 629, 2662, 4207, 5575, 5920
TspGWI ACGGA 3 cut(s) 1685, 2170, 3987
Vha464I CTTAAG 1 cut(s) 5483
VneI GTGCAC 2 cut(s) 3024, 3260
VpaK11BI GGWCC 7 cut(s) 89, 356, 1749, 3280, 3401, 4635, 6407
VspI ATTAAT 3 cut(s) 57, 1835, 1940
XbaI TCTAGA 1 cut(s) 5311
XcmI CCANNNNNNNNNTGG 1 cut(s) 1273
XhoI CTCGAG 2 cut(s) 2864, 5811
XmiI GTMKAC 3 cut(s) 3575, 4723, 5781
XmnI GAANNNNTTC 4 cut(s) 1059, 1375, 5319, 6648
ZraI GACGTC 1 cut(s) 634
Zsp2I ATGCAT 5 cut(s) 3860, 4856, 5100, 5353, 6117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.