Rorug05G0572700

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003459
Physical Location & Seq
Reverse (-)
76598759 .. 76604473
5715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0572700.1

Sequence Viewer

Length: 4041 bp
ATGGCCTCGACCTCCCGAGAAACTGCTGCATCTCTTCCTTCATCAAATGAAGCATCATCAACGGCTCGTCGCTGGAAGGATGATGTGTTTTTGAGTTACAAGAGTGGTGACACTCCAAAGGGCTTTATATTCCATTTATACGATCGGCTGCAACAGAGGAAAATCACAACATTCATGGATCATTCAGATTTTCAAGTAGGGGATGCTATCTCTCCCTCTCTCCTAAAGGCAATTGAAGAATCAAAGTTCGCAGTTGTTGTTCTGTCCCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTACAAAGATTTGTCAGTTCATAGAGGACGGCTTTACAATTCTTCCAGTTTTTTATGAGTTCGACCCTATTAATGTAAGAAATCAAAAGGGAAGTTTTCAGGATGCTTTCGCCAAGCACGAAAGGTCTGGGCGACACCAACCGCAGAAGATCCAGCAGTGGAGAGATGCTTTAACAAAAGTGGCCAGTTTGTCTGGGTTGAATACACAGGATTACAGGACTGACAATGATCTTGTCGAAGCCATTGTGGAATCTATGTGCAGTCAAATGAAGACTATTGAAATTGTGCATACAATTACGGAGTCAGATGAAGATTGCATTGTGGAATCGGAGTCAGATGAAGATTCCACTGTGGAACCTGTGTGCAGTCAAATGGAGACTATTGAAATTAAGCCTACAATGCCAAAGGATTTTGAAGCAGTTGAAGCAACAACACAAGCCACCAATGGAAACTCTGAACCAGCTCCTCAGTGGAAGCATGATGTGTTTTTGAGCTTTAGGGGTGATGACACTCGCAAGGGTTTTCTATCCCATTTATACCATGAATTACAAAACACGAAAGTAATTAGAACATTTAAGGATGATGAACAACTTAAGAAAGGGAAGGATATTTCTCAAAGTCTGCTGACGGCAATTGAAGAATCAAGGTTTGCGATCATCGTTCTGTCAGAAGACTATGCTTCTTCTGCATGGTGTTTAGATGAACTTTCAAAGATTTTTCAATGCATGGAAGGCAAGGATACAATTCTGCCGATTTTTTATCATGTGGATCCCTCTGATGTACGATATCAAAACAATAAACTCGAAACGGCCTTTACAAAGCATTCAAGCAAGGAAAGGTATGACATAGAGAAGGTGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGAAATCTCTCTGGGTGGGATTCAAAGAACCACAAATCTGAAAGGGAACTCGTTGAAGACGTAGTGAAATTTGTGCTCAAAGAAGTACTTCAAGAACCAAGGGGAGATTTTGAAGAATTTGAAGCAACAACAGAAGCCATGGATGAGGTTAAAAAGATGCTGAAAGATGATAAGATCACCGCCATTGGGGTCTATGGAAGAGGAGGCGTTGGCAAGACAGCCATGGTGAAACATGTCGGTGCACAAGCCAAAAGAATTGGGCTTTTTGATCACGTTGTTATGGCTGTTATATCCCGAAGCCCTGATTTGCAAAAAATTCAAGGCGCATTGGTAAATCAGCTGGGATTGGAATTGCTGGAGAATGAACAAACAGAAAGAGCCAGTAGATTGAAGGCGAGGATCATGGGAGGAAAGAGGATCCTTATTATCTTGGACGACATTTGGGAGATGATAGATTTGTCAAGCATTGGAATTCCTAGCTATGACGAGCTTCAGAGATGCAATTCCAAGGTCCTACTCACCACAAGGATCAGGAATCTTTGTCATTCTATGGGATGCCAAGCTCATATTCCTCTCAATATCCTATCAGAGCAAGATTCTTTGAAGTTATTCATGAAGAAGTCAAGAAAGCCTTTTCATGAATCTACTGATTCCTATGATGAAGAGAGGAACATAGTTAGAGAATGCGCTGGTCTCCCAAATGTATTGGAAGAAGCTGCAAGGGCACTTGAACATAAATACGTGGAAGAATGGAACGAAGCAGTTTCAAAGGCATTTGGAGATGATGACGATGAATTGCAAAAAGCAGCTCAAGAACTACATGCTTCTCAACTTGTCCACACTAAAGATGAGAGAGATACATTTAGAAGAATAAAGTTAACCTATCATACCTTGAGGTTTGATGATGCCAAGTCATTTTTCTTGCTTTGCTGCATGTTTCCAGAAGATTATGATATCCCAATTGAAGAATTTTTGAAGTATGGGATTGGAAAAGGTTTGCTTCAATATTCAGACACATTGCAAGAAGCTAGAGACAGAGCACATTCTTTGGTCGCATACCTTAAAGCTTCGGGGTTGCTTTTGGATGGCAAGAAGGGATGCGTAAGGATGCATTGTGACATCCGAAATGTGGCCATGTCTATTGCATTGTCTGAAGAAGGTCAGGCTATTTACGTAAAAGCTGGTTGTGAATTGAAGGATTGGCCAATGGATGCTCTTGAGGGCTACTCAGCAATCTCACTAATGAGGAACAAACTTAACAAGCTTCCCGAAACTTTGGTATGTTCTGAACTCCAAATCTTACTGCTGCAAAGTAATGCTGGTATATGTGACATTCCAAAAACTTTTTTTCAAAGTCCAAATGCACTAAGGGTCATTGATCTCAGCTGTACCGGTATTTCAGTACTACCCTCTTCATTCAATGTCCTCACCAACCTCCAAGCCTTGTTTTTGGACTGTTGCAATAAAATAACTGACATTTCCGTACTTAGAAAGCTGAAGAAAGTTGAGATTCTCAGTTTGAGAAAATTTCCTCATAAGGAGCTGCCAAAAGAGATAGGAAATTTGACCAGTCTAAGGATGCTGGACGTCACTGGTGGATGTGTTCACAAAATTCCATCTGAAGTGATATCGAGGTTGTATAGACTCGAAGAATTGTACATGAAATGTGAATTTGGGAACTGGGGAAGTAAAGTCGAGGAAGCAGTAGGACAAACAAATGCCGACTTTGATGAGATAATTGGCTTGTCCCGTTTAAACATTTTGAAGGTTTACATATCTGATGCAGCTTGCTTACCCAAAGATATTGGGTACTTTGTGCCGAATTGGGTAAAATTTGACATATGCATCAGCAGAGATCTCTCTTCGGAGATGATGCTATCTCCGGATGATCAACCTTCAAGAACCTTGACTCTTGACAGAACAATTAATGGCTTATCGGATTGGTTTATCAATGTGGTGACGGAGACAGCAATAAAACTAGAGTACAGAGAGTGTAAGGGATTAAAAAACATTCTTAAGGAATATGAGCATGGGAGATTACATGGGCTCAAGCATCTCTCTGTAATTGGGTGCCATGAGAACTTGGAAGAACTGATAAATGTAACAACATGGGATCCAAGTAAACCTGCGTTCGAGAGCTTAGAAGTGTTGCATCTCCTTCACTTGCAACGCCTCAAGGAGTTATGTGTTGGCGAATTACCACTTGGGTCTCTATGCAATCTCAAGTTATTGAAAGTGCAAACTTGTTGCAACTTGGTGAATGCACTTTTACCACCAAAATTGTTGCAGAGACTACCAAAACTAGAAACAATATTTTGTGTGAATATGGAACACATGAAATATGTGTTTGGATCTGAAGGGCTTGAGCCTGGAATGATTCTCTTGACAAAATTGAGCGAGATGAGACTGTGCGCTCTCTTCGAACTAATCAGCATCTGGAATGGTCCTGCTCCACATGCAGTCTTTCGTAATCTTAAAGTTTTGGCAGTCTGCATGTGTACAAAACTGAAATATCTATTCCCGTTCGATGTAGCTCGATCTCTTGTGCAATTGGAAGACCTCTCTGTAGAGCATTGCCTTAACTTGGGCCGAGTTATTGAAGCAAGCAACAGCTTGAACAGATATATCGTTTTTCTAGAATTGAAGAAATTAGTTTTGATAAATCTTCCAGAGCTCACATGGTTCTGCACCGAAAATGGTACTGTTGATATCGAGTGCCCTTTGTTGGAATACTTGTATGTGGATAAGTGCCCCCAGTTTAAAAACTCTGCTTCTGCTTCTGTTAAACTCAATGATCAACAACATTTGAATTCATTAGTGCAAAGATGGGAGGATATATATAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

1346

Amino Acids

153.02

Weight (kDa)

5.56

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 28 - 192 4.2e-44 TIR domain
TIR_2 PF13676 29 - 124 7.8e-16 TIR domain
TIR PF01582 260 - 429 5.1e-50 TIR domain
TIR_2 PF13676 263 - 359 4.4e-15 TIR domain
NB-ARC PF00931 442 - 605 1.5e-28 NB-ARC domain
LRR_8 PF13855 827 - 883 1.1e-06 Leucine rich repeat
LRR_14 PF23598 889 - 952 1.3e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 1113 - 1256 1e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2781
Acc36I ACCTGC 1 cut(s) 3325
AccB1I GGYRCC 1 cut(s) 3261
AccIII TCCGGA 1 cut(s) 3073
AciI CCGC 2 cut(s) 446, 1371
AcoI YGGCCR 3 cut(s) 486, 2322, 2393
AcuI CTGAAG 5 cut(s) 1667, 2364, 2708, 2832, 3567
AcyI GRCGYC 1 cut(s) 2778
AfiI CCNNNNNNNGG 5 cut(s) 1377, 2320, 2766, 3775, 3916
AflII CTTAAG 2 cut(s) 896, 3206
AflIII ACRYGT 1 cut(s) 1423
AgeI ACCGGT 1 cut(s) 2582
AjnI CCWGG 1 cut(s) 3559
AjuI GAANNNNNNNTTGG 6 cut(s) 1743, 1775, 2093, 2125, 3378, 3410
Alw21I GWGCWC 4 cut(s) 1269, 1435, 2233, 3867
Alw26I GTCTC 7 cut(s) 674, 1889, 2217, 3149, 3405, 3475, 3589
Alw44I GTGCAC 1 cut(s) 1431
AlwNI CAGNNNCTG 1 cut(s) 3627
Ama87I CYCGRG 1 cut(s) 15
Aor13HI TCCGGA 1 cut(s) 3073
AoxI GGCC 6 cut(s) 3, 486, 1113, 2322, 2393, 3778
ApaLI GTGCAC 1 cut(s) 1431
ApeKI GCWGC 8 cut(s) 26, 148, 1907, 1997, 2121, 2497, 2734, 2975
AseI ATTAAT 2 cut(s) 375, 3117
AsiGI ACCGGT 1 cut(s) 2582
Asp700I GAANNNNTTC 2 cut(s) 1278, 1799
AspLEI GCGC 3 cut(s) 1517, 1880, 3605
AspS9I GGNCC 3 cut(s) 1702, 3635, 3778
AsuHPI GGTGA 9 cut(s) 119, 818, 1171, 1360, 1429, 1702, 2611, 3160, 3460
AsuII TTCGAA 1 cut(s) 3612
AvaI CYCGRG 1 cut(s) 15
AvaII GGWCC 2 cut(s) 1702, 3635
BaeGI GKGCMC 4 cut(s) 1435, 1918, 3911, 3944
BalI TGGCCA 3 cut(s) 488, 2324, 2395
BamHI GGATCC 3 cut(s) 1072, 1608, 3304
BanI GGYRCC 1 cut(s) 3261
BanII GRGCYC 2 cut(s) 3240, 3867
BbsI GAAGAC 4 cut(s) 581, 981, 1254, 3753
Bbv12I GWGCWC 4 cut(s) 1269, 1435, 2233, 3867
BbvI GCAGC 8 cut(s) 13, 135, 1894, 2009, 2108, 2484, 2721, 2987
BccI CCATC 3 cut(s) 2270, 2815, 4011
BceAI ACGGC 4 cut(s) 78, 349, 948, 1128
BciT130I CCWGG 1 cut(s) 3561
BciVI GTATCC 1 cut(s) 1036
BclI TGATCA 3 cut(s) 1459, 3079, 3985
BcoDI GTCTC 7 cut(s) 674, 1889, 2217, 3149, 3405, 3475, 3589
BfaI CTAG 5 cut(s) 1668, 2220, 3170, 3494, 3827
BfmI CTRYAG 1 cut(s) 3756
BfrI CTTAAG 2 cut(s) 896, 3206
BfuAI ACCTGC 1 cut(s) 3325
BfuI GTATCC 1 cut(s) 1036
BglII AGATCT 1 cut(s) 3046
BisI GCNGC 8 cut(s) 27, 149, 1908, 1998, 2122, 2498, 2735, 2976
BlsI GCNGC 8 cut(s) 28, 150, 1909, 1999, 2123, 2499, 2736, 2977
BmcAI AGTACT 2 cut(s) 1278, 2595
Bme1390I CCNGG 1 cut(s) 3561
Bme18I GGWCC 2 cut(s) 1702, 3635
BmeT110I CYCGRG 1 cut(s) 15
BmgT120I GGNCC 3 cut(s) 1702, 3635, 3778
BmiI GGNNCC 5 cut(s) 660, 1074, 1610, 3263, 3306
BmrFI CCNGG 1 cut(s) 3561
BmrI ACTGGG 2 cut(s) 2881, 3940
BmuI ACTGGG 2 cut(s) 2881, 3940
BpiI GAAGAC 4 cut(s) 581, 981, 1254, 3753
BplI GAGNNNNNCTC 2 cut(s) 2402, 2434
BpmI CTGGAG 1 cut(s) 1568
Bpu14I TTCGAA 1 cut(s) 3612
BpuEI CTTGAG 7 cut(s) 1986, 2104, 2429, 3224, 3350, 3398, 3575
BsaAI YACGTR 2 cut(s) 1933, 2365
BsaHI GRCGYC 1 cut(s) 2778
BsaI GGTCTC 2 cut(s) 1889, 3405
BsaJI CCNNGG 4 cut(s) 1289, 1329, 1413, 1698
BsaWI WCCGGW 2 cut(s) 2582, 3073
Bsc4I CCNNNNNNNGG 5 cut(s) 1377, 2320, 2766, 3775, 3916
Bse118I RCCGGY 1 cut(s) 2582
Bse1I ACTGG 7 cut(s) 350, 489, 1572, 2760, 2788, 2876, 3946
Bse3DI GCAATG 2 cut(s) 2207, 3763
BseAI TCCGGA 1 cut(s) 3073
BseBI CCWGG 1 cut(s) 3561
BseDI CCNNGG 4 cut(s) 1289, 1329, 1413, 1698
BseLI CCNNNNNNNGG 5 cut(s) 1377, 2320, 2766, 3775, 3916
BseMI GCAATG 2 cut(s) 2207, 3763
BseMII CTCAG 4 cut(s) 785, 2433, 2587, 2719
BseNI ACTGG 7 cut(s) 350, 489, 1572, 2760, 2788, 2876, 3946
BseRI GAGGAG 2 cut(s) 759, 1407
BseSI GKGCMC 4 cut(s) 1435, 1918, 3911, 3944
BseXI GCAGC 8 cut(s) 13, 135, 1894, 2009, 2108, 2484, 2721, 2987
BseYI CCCAGC 1 cut(s) 1531
BsgI GTGCAG 3 cut(s) 583, 688, 3862
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 6 cut(s) 5, 488, 1115, 2324, 2395, 3780
BshNI GGYRCC 1 cut(s) 3261
BshTI ACCGGT 1 cut(s) 2582
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 4 cut(s) 1269, 1435, 2233, 3867
BsiHKCI CYCGRG 1 cut(s) 15
BsiSI CCGG 2 cut(s) 2583, 3074
BslFI GGGAC 2 cut(s) 250, 2923
BslI CCNNNNNNNGG 5 cut(s) 1377, 2320, 2766, 3775, 3916
BsmAI GTCTC 7 cut(s) 674, 1889, 2217, 3149, 3405, 3475, 3589
BsmFI GGGAC 2 cut(s) 250, 2923
BsmI GAATGC 3 cut(s) 1126, 1880, 3457
BsnI GGCC 6 cut(s) 5, 488, 1115, 2324, 2395, 3780
Bso31I GGTCTC 2 cut(s) 1889, 3405
BsoBI CYCGRG 1 cut(s) 15
Bsp119I TTCGAA 1 cut(s) 3612
Bsp1286I GDGCHC 8 cut(s) 1269, 1435, 1918, 2233, 3240, 3867, 3911, 3944
Bsp13I TCCGGA 1 cut(s) 3073
Bsp1407I TGTACA 2 cut(s) 2847, 3689
Bsp19I CCATGG 2 cut(s) 1329, 1413
BspACI CCGC 2 cut(s) 446, 1371
BspANI GGCC 6 cut(s) 5, 488, 1115, 2324, 2395, 3780
BspCNI CTCAG 4 cut(s) 784, 2432, 2586, 2718
BspEI TCCGGA 1 cut(s) 3073
BspHI TCATGA 2 cut(s) 1803, 1828
BspLI GGNNCC 5 cut(s) 660, 1074, 1610, 3263, 3306
BspMI ACCTGC 1 cut(s) 3325
BspT104I TTCGAA 1 cut(s) 3612
BspT107I GGYRCC 1 cut(s) 3261
BspTI CTTAAG 2 cut(s) 896, 3206
BspTNI GGTCTC 2 cut(s) 1889, 3405
BsrDI GCAATG 2 cut(s) 2207, 3763
BsrFI RCCGGY 1 cut(s) 2582
BsrGI TGTACA 2 cut(s) 2847, 3689
BsrI ACTGG 7 cut(s) 350, 489, 1572, 2760, 2788, 2876, 3946
BssAI RCCGGY 1 cut(s) 2582
BssECI CCNNGG 4 cut(s) 1289, 1329, 1413, 1698
BssNI GRCGYC 1 cut(s) 2778
BssT1I CCWWGG 4 cut(s) 1289, 1329, 1413, 1698
Bst2UI CCWGG 1 cut(s) 3561
Bst4CI ACNGT 4 cut(s) 655, 2648, 3600, 3895
Bst6I CTCTTC 6 cut(s) 39, 1384, 1848, 2608, 3058, 3614
BstACI GRCGYC 1 cut(s) 2778
BstAFI CTTAAG 2 cut(s) 896, 3206
BstAUI TGTACA 2 cut(s) 2847, 3689
BstBAI YACGTR 2 cut(s) 1933, 2365
BstBI TTCGAA 1 cut(s) 3612
BstC8I GCNNGC 2 cut(s) 2980, 3796
BstDEI CTNAG 8 cut(s) 771, 2419, 2558, 2573, 2678, 2705, 2765, 3331
BstDSI CCRYGG 2 cut(s) 1329, 1413
BstHHI GCGC 3 cut(s) 1517, 1880, 3605
BstMAI GTCTC 7 cut(s) 674, 1889, 2217, 3149, 3405, 3475, 3589
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 6 cut(s) 703, 728, 989, 1035, 1913, 3647
BstNI CCWGG 1 cut(s) 3561
BstNSI RCATGY 5 cut(s) 1427, 2015, 2128, 3650, 3688
BstSCI CCNGG 1 cut(s) 3559
BstSFI CTRYAG 1 cut(s) 3756
BstSLI GKGCMC 4 cut(s) 1435, 1918, 3911, 3944
BstSNI TACGTA 1 cut(s) 2365
BstV1I GCAGC 8 cut(s) 13, 135, 1894, 2009, 2108, 2484, 2721, 2987
BstV2I GAAGAC 4 cut(s) 581, 981, 1254, 3753
BstX2I RGATCY 6 cut(s) 453, 1072, 1608, 3046, 3304, 3542
BstYI RGATCY 6 cut(s) 453, 1072, 1608, 3046, 3304, 3542
BsuI GTATCC 1 cut(s) 1036
BsuRI GGCC 6 cut(s) 5, 488, 1115, 2324, 2395, 3780
BtgI CCRYGG 2 cut(s) 1329, 1413
BtsI GCAGTG 2 cut(s) 467, 1172
BtsIMutI CAGTG 5 cut(s) 467, 651, 779, 1172, 2781
BveI ACCTGC 1 cut(s) 3325
Cac8I GCNNGC 2 cut(s) 2980, 3796
CaiI CAGNNNCTG 1 cut(s) 3627
CciI TCATGA 2 cut(s) 1803, 1828
CfoI GCGC 3 cut(s) 1517, 1880, 3605
Cfr10I RCCGGY 1 cut(s) 2582
Cfr13I GGNCC 3 cut(s) 1702, 3635, 3778
CspAI ACCGGT 1 cut(s) 2582
CspCI CAANNNNNGTGG 2 cut(s) 3453, 3488
DdeI CTNAG 8 cut(s) 771, 2419, 2558, 2573, 2678, 2705, 2765, 3331
DraI TTTAAA 2 cut(s) 2946, 3952
EaeI YGGCCR 3 cut(s) 486, 2322, 2393
Eam1104I CTCTTC 6 cut(s) 39, 1384, 1848, 2608, 3058, 3614
EarI CTCTTC 6 cut(s) 39, 1384, 1848, 2608, 3058, 3614
Ecl136II GAGCTC 1 cut(s) 3865
Eco105I TACGTA 1 cut(s) 2365
Eco130I CCWWGG 4 cut(s) 1289, 1329, 1413, 1698
Eco24I GRGCYC 2 cut(s) 3240, 3867
Eco31I GGTCTC 2 cut(s) 1889, 3405
Eco32I GATATC 4 cut(s) 1091, 2146, 2820, 3901
Eco47I GGWCC 2 cut(s) 1702, 3635
Eco53kI GAGCTC 1 cut(s) 3865
Eco57I CTGAAG 5 cut(s) 1667, 2364, 2708, 2832, 3567
Eco88I CYCGRG 1 cut(s) 15
EcoICRI GAGCTC 1 cut(s) 3865
EcoO109I RGGNCCY 1 cut(s) 1702
EcoRI GAATTC 2 cut(s) 1662, 4000
EcoRII CCWGG 1 cut(s) 3559
EcoRV GATATC 4 cut(s) 1091, 2146, 2820, 3901
EcoT14I CCWWGG 4 cut(s) 1289, 1329, 1413, 1698
EcoT22I ATGCAT 3 cut(s) 1031, 2304, 3038
EcoT38I GRGCYC 2 cut(s) 3240, 3867
ErhI CCWWGG 4 cut(s) 1289, 1329, 1413, 1698
FalI AAGNNNNNCTT 6 cut(s) 1263, 1295, 1807, 1839, 2175, 2207
FaqI GGGAC 2 cut(s) 250, 2923
FauNDI CATATG 1 cut(s) 3032
FbaI TGATCA 3 cut(s) 1459, 3079, 3985
Fnu4HI GCNGC 8 cut(s) 27, 149, 1908, 1998, 2122, 2498, 2735, 2976
FriOI GRGCYC 2 cut(s) 3240, 3867
Fsp4HI GCNGC 8 cut(s) 27, 149, 1908, 1998, 2122, 2498, 2735, 2976
FspBI CTAG 5 cut(s) 1668, 2220, 3170, 3494, 3827
GlaI GCGC 3 cut(s) 1516, 1879, 3604
GluI GCNGC 8 cut(s) 27, 149, 1908, 1998, 2122, 2498, 2735, 2976
GsaI CCCAGC 1 cut(s) 1535
GsuI CTGGAG 1 cut(s) 1568
HaeIII GGCC 6 cut(s) 5, 488, 1115, 2324, 2395, 3780
HapII CCGG 2 cut(s) 2583, 3074
HhaI GCGC 3 cut(s) 1517, 1880, 3605
Hin1I GRCGYC 1 cut(s) 2778
Hin6I GCGC 3 cut(s) 1515, 1878, 3603
HinP1I GCGC 3 cut(s) 1515, 1878, 3603
HincII GTYRAC 1 cut(s) 2070
HindII GTYRAC 1 cut(s) 2070
HindIII AAGCTT 3 cut(s) 2256, 2453, 4034
HpaI GTTAAC 1 cut(s) 2070
HpaII CCGG 2 cut(s) 2583, 3074
HphI GGTGA 9 cut(s) 119, 818, 1171, 1360, 1429, 1702, 2611, 3160, 3460
Hpy166II GTNNAC 7 cut(s) 1433, 2029, 2070, 2797, 2962, 3314, 3690
Hpy8I GTNNAC 7 cut(s) 1433, 2029, 2070, 2797, 2962, 3314, 3690
Hpy99I CGWCG 1 cut(s) 72
HpyCH4III ACNGT 4 cut(s) 655, 2648, 3600, 3895
HpyCH4IV ACGT 5 cut(s) 1251, 1464, 1932, 2364, 2778
HpyF10VI GCNNNNNNNGC 6 cut(s) 703, 728, 989, 1035, 1913, 3647
HpyF3I CTNAG 8 cut(s) 771, 2419, 2558, 2573, 2678, 2705, 2765, 3331
HpySE526I ACGT 5 cut(s) 1251, 1464, 1932, 2364, 2778
Hsp92I GRCGYC 1 cut(s) 2778
HspAI GCGC 3 cut(s) 1515, 1878, 3603
Kpn2I TCCGGA 1 cut(s) 3073
Ksp22I TGATCA 3 cut(s) 1459, 3079, 3985
KspAI GTTAAC 1 cut(s) 2070
LmnI GCTCC 3 cut(s) 772, 2731, 3646
Lsp1109I GCAGC 8 cut(s) 13, 135, 1894, 2009, 2108, 2484, 2721, 2987
MaeI CTAG 5 cut(s) 1668, 2220, 3170, 3494, 3827
MaeII ACGT 5 cut(s) 1251, 1464, 1932, 2364, 2778
MaeIII GTNAC 7 cut(s) 95, 107, 2306, 2519, 2779, 3148, 3292
MfeI CAATTG 4 cut(s) 231, 936, 2151, 3740
MflI RGATCY 6 cut(s) 453, 1072, 1608, 3046, 3304, 3542
MhlI GDGCHC 8 cut(s) 1269, 1435, 1918, 2233, 3240, 3867, 3911, 3944
MlsI TGGCCA 3 cut(s) 488, 2324, 2395
MluNI TGGCCA 3 cut(s) 488, 2324, 2395
MlyI GAGTC 4 cut(s) 614, 644, 2829, 3094
MmeI TCCRAC 1 cut(s) 3897
Mox20I TGGCCA 3 cut(s) 488, 2324, 2395
Mph1103I ATGCAT 3 cut(s) 1031, 2304, 3038
MroI TCCGGA 1 cut(s) 3073
MroXI GAANNNNTTC 2 cut(s) 1278, 1799
MscI TGGCCA 3 cut(s) 488, 2324, 2395
MslI CAYNNNNRTG 1 cut(s) 1428
Msp20I TGGCCA 3 cut(s) 488, 2324, 2395
MspA1I CMGCKG 2 cut(s) 1531, 2577
MspCI CTTAAG 2 cut(s) 896, 3206
MspI CCGG 2 cut(s) 2583, 3074
MspR9I CCNGG 1 cut(s) 3561
MssI GTTTAAAC 1 cut(s) 2946
MunI CAATTG 4 cut(s) 231, 936, 2151, 3740
Mva1269I GAATGC 3 cut(s) 1126, 1880, 3457
MvaI CCWGG 1 cut(s) 3561
MwoI GCNNNNNNNGC 6 cut(s) 703, 728, 989, 1035, 1913, 3647
NcoI CCATGG 2 cut(s) 1329, 1413
NdeI CATATG 1 cut(s) 3032
NlaIV GGNNCC 5 cut(s) 660, 1074, 1610, 3263, 3306
NmeAIII GCCGAG 1 cut(s) 3806
NmuCI GTSAC 5 cut(s) 107, 2306, 2519, 2779, 3148
NsiI ATGCAT 3 cut(s) 1031, 2304, 3038
NspI RCATGY 5 cut(s) 1427, 2015, 2128, 3650, 3688
NspV TTCGAA 1 cut(s) 3612
PagI TCATGA 2 cut(s) 1803, 1828
PciI ACATGT 1 cut(s) 1423
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 1248
PctI GAATGC 3 cut(s) 1126, 1880, 3457
PdmI GAANNNNTTC 2 cut(s) 1278, 1799
PinAI ACCGGT 1 cut(s) 2582
PkrI GCNGC 8 cut(s) 28, 150, 1909, 1999, 2123, 2499, 2736, 2977
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 4 cut(s) 613, 643, 2829, 3094
PmeI GTTTAAAC 1 cut(s) 2946
PpsI GAGTC 4 cut(s) 613, 643, 2829, 3094
Ppu21I YACGTR 2 cut(s) 1933, 2365
PpuMI RGGWCCY 1 cut(s) 1702
PscI ACATGT 1 cut(s) 1423
PshBI ATTAAT 2 cut(s) 375, 3117
Psp124BI GAGCTC 1 cut(s) 3867
Psp5II RGGWCCY 1 cut(s) 1702
Psp6I CCWGG 1 cut(s) 3559
PspFI CCCAGC 1 cut(s) 1531
PspGI CCWGG 1 cut(s) 3559
PspN4I GGNNCC 5 cut(s) 660, 1074, 1610, 3263, 3306
PspPI GGNCC 3 cut(s) 1702, 3635, 3778
PspPPI RGGWCCY 1 cut(s) 1702
PstNI CAGNNNCTG 1 cut(s) 3627
PsuI RGATCY 6 cut(s) 453, 1072, 1608, 3046, 3304, 3542
PvuI CGATCG 1 cut(s) 145
PvuII CAGCTG 2 cut(s) 1531, 2577
RseI CAYNNNNRTG 1 cut(s) 1428
SacI GAGCTC 1 cut(s) 3867
SatI GCNGC 8 cut(s) 27, 149, 1908, 1998, 2122, 2498, 2735, 2976
Sau96I GGNCC 3 cut(s) 1702, 3635, 3778
ScaI AGTACT 2 cut(s) 1278, 2595
SchI GAGTC 4 cut(s) 614, 644, 2829, 3094
ScrFI CCNGG 1 cut(s) 3561
SduI GDGCHC 8 cut(s) 1269, 1435, 1918, 2233, 3240, 3867, 3911, 3944
SfcI CTRYAG 1 cut(s) 3756
SfuI TTCGAA 1 cut(s) 3612
SinI GGWCC 2 cut(s) 1702, 3635
SmiMI CAYNNNNRTG 1 cut(s) 1428
SmlI CTYRAG 9 cut(s) 896, 2001, 2083, 2408, 3206, 3239, 3365, 3413, 3554
SmoI CTYRAG 9 cut(s) 896, 2001, 2083, 2408, 3206, 3239, 3365, 3413, 3554
SnaBI TACGTA 1 cut(s) 2365
SsiI CCGC 2 cut(s) 446, 1371
SspI AATATT 2 cut(s) 2198, 3504
SspMI CTAG 5 cut(s) 1668, 2220, 3170, 3494, 3827
SstI GAGCTC 1 cut(s) 3867
StyD4I CCNGG 1 cut(s) 3559
StyI CCWWGG 4 cut(s) 1289, 1329, 1413, 1698
TaaI ACNGT 4 cut(s) 655, 2648, 3600, 3895
TaiI ACGT 5 cut(s) 1254, 1467, 1935, 2367, 2781
TatI WGTACW 5 cut(s) 1276, 2593, 2847, 3174, 3689
TscAI CASTG 5 cut(s) 467, 658, 779, 1172, 2788
TseFI GTSAC 5 cut(s) 107, 2306, 2519, 2779, 3148
TseI GCWGC 8 cut(s) 26, 148, 1907, 1997, 2121, 2497, 2734, 2975
Tsp45I GTSAC 5 cut(s) 107, 2306, 2519, 2779, 3148
TspGWI ACGGA 3 cut(s) 617, 2662, 3167
TspRI CASTG 5 cut(s) 467, 658, 779, 1172, 2788
Vha464I CTTAAG 2 cut(s) 896, 3206
VneI GTGCAC 1 cut(s) 1431
VpaK11BI GGWCC 2 cut(s) 1702, 3635
VspI ATTAAT 2 cut(s) 375, 3117
XbaI TCTAGA 1 cut(s) 3826
XceI RCATGY 5 cut(s) 1427, 2015, 2128, 3650, 3688
XcmI CCANNNNNNNNNTGG 2 cut(s) 771, 3867
XmnI GAANNNNTTC 2 cut(s) 1278, 1799
XspI CTAG 5 cut(s) 1668, 2220, 3170, 3494, 3827
ZraI GACGTC 1 cut(s) 2779
ZrmI AGTACT 2 cut(s) 1278, 2595
Zsp2I ATGCAT 3 cut(s) 1031, 2304, 3038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.