RchiOBHm_Chr7g0226601

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001715
Physical Location & Seq
Reverse (-)
49842169 .. 49843929
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20295

Sequence Viewer

Length: 627 bp
ATGGCTAGGGTTTTCGTCCAGTCTACTAACATTCCTAGTGTGGTTGCCAGGCCAAAGCTTGGTCAATTTAAAGGATCTGAAAATTCTAGAAAGGCAACAAAGATGATGTGTACTTTACGAGCACCCGCATTGAGATTGAGCAGTTTCTCAGGACTCCGTAGTGTTAATGTTTTAGATATTATGGTGAAGCCTGGGCAAGACTTATATGCTAAGGTGGGAGAAGCGATCTCCTCCCGGCATCGAAAGGCTAGCAGATGTGTGCCTAAAGCCACGTCGTTTGATCGCTTCTCAGAAAAGGCAATAAAAGCGATTATGCTTGCACAAGAGGAAACAATGCGAATTGGTCACAACTTTGTTGGTACAGAGCACATACTTTTGGGTCTTGTTGGTGAAGGAACTGGGATTGCTGCCAAGGTCCTTAAATCTATTGGAATCAATCTTAAAGGTGCACGCGTTGAAGTGAAAAAGATCATTGGAACGGGTCGTGGCTTTGTTGCTATTGAAATCCCATTTACTTCTCGTGCAAAGCGAGTTTTGGAACTGTCACAGGAGGAAGCTCGACAACTGGGTATATATTATTTTTCCATGTCCTTTAAATATCTTTCACAAAAACATGATAGTACTTAA

Protein Analysis

208

Amino Acids

22.89

Weight (kDa)

10.31

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 95 - 193 3.2e-22 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 59
AccI GTMKAC 1 cut(s) 23
AccII CGCG 1 cut(s) 453
AciI CCGC 1 cut(s) 126
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 82
AfaI GTAC 3 cut(s) 112, 361, 622
AfiI CCNNNNNNNGG 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 2 cut(s) 458, 503
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 2 cut(s) 47, 190
AluBI AGCT 2 cut(s) 58, 557
AluI AGCT 2 cut(s) 58, 557
Alw21I GWGCWC 3 cut(s) 124, 369, 451
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 1 cut(s) 82
AoxI GGCC 1 cut(s) 50
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 196, 401
AsuNHI GCTAGC 1 cut(s) 248
AvaII GGWCC 1 cut(s) 415
BaeGI GKGCMC 1 cut(s) 451
BauI CACGAG 1 cut(s) 519
Bbv12I GWGCWC 3 cut(s) 124, 369, 451
BbvI GCAGC 1 cut(s) 394
BciT130I CCWGG 2 cut(s) 49, 192
BcnI CCSGG 1 cut(s) 235
BfaI CTAG 4 cut(s) 6, 36, 87, 249
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
BmcAI AGTACT 1 cut(s) 622
Bme1390I CCNGG 3 cut(s) 49, 192, 235
Bme18I GGWCC 1 cut(s) 415
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 415
BmrFI CCNGG 3 cut(s) 49, 192, 235
BmrI ACTGGG 2 cut(s) 408, 575
BmsI GCATC 1 cut(s) 247
BmtI GCTAGC 1 cut(s) 252
BmuI ACTGGG 2 cut(s) 408, 575
Bpu10I CCTNAGC 1 cut(s) 210
BpuMI CCSGG 1 cut(s) 235
BsaJI CCNNGG 2 cut(s) 191, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 59
Bse1I ACTGG 3 cut(s) 19, 403, 570
BseBI CCWGG 2 cut(s) 49, 192
BseDI CCNNGG 2 cut(s) 191, 411
BseLI CCNNNNNNNGG 1 cut(s) 59
BseMII CTCAG 2 cut(s) 162, 303
BseNI ACTGG 3 cut(s) 19, 403, 570
BseRI GAGGAG 1 cut(s) 220
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 453
BshFI GGCC 1 cut(s) 52
BsiHKAI GWGCWC 3 cut(s) 124, 369, 451
BsiSI CCGG 1 cut(s) 235
BslI CCNNNNNNNGG 1 cut(s) 59
BsnI GGCC 1 cut(s) 52
Bsp1286I GDGCHC 3 cut(s) 124, 369, 451
Bsp143I GATC 4 cut(s) 74, 225, 280, 468
BspACI CCGC 1 cut(s) 126
BspANI GGCC 1 cut(s) 52
BspCNI CTCAG 2 cut(s) 161, 302
BspFNI CGCG 1 cut(s) 453
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 1 cut(s) 82
BsrI ACTGG 3 cut(s) 19, 403, 570
BssECI CCNNGG 2 cut(s) 191, 411
BssMI GATC 4 cut(s) 74, 225, 280, 468
BssSI CACGAG 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 2 cut(s) 49, 192
Bst4CI ACNGT 1 cut(s) 543
BstC8I GCNNGC 3 cut(s) 250, 318, 451
BstDEI CTNAG 3 cut(s) 148, 210, 289
BstFNI CGCG 1 cut(s) 453
BstKTI GATC 4 cut(s) 77, 228, 283, 471
BstMBI GATC 4 cut(s) 74, 225, 280, 468
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 2 cut(s) 49, 192
BstSCI CCNGG 3 cut(s) 47, 190, 233
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 1 cut(s) 453
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BsuRI GGCC 1 cut(s) 52
BtrI CACGTC 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 250, 318, 451
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 3 cut(s) 111, 360, 621
CspCI CAANNNNNGTGG 2 cut(s) 259, 294
CviAII CATG 2 cut(s) 586, 614
CviJI RGCY 8 cut(s) 5, 52, 58, 190, 248, 269, 489, 557
CviKI_1 RGCY 8 cut(s) 5, 52, 58, 190, 248, 269, 489, 557
CviQI GTAC 3 cut(s) 111, 360, 621
DdeI CTNAG 3 cut(s) 148, 210, 289
DpnI GATC 4 cut(s) 76, 227, 282, 470
DpnII GATC 4 cut(s) 74, 225, 280, 468
DraI TTTAAA 2 cut(s) 70, 595
Eco130I CCWWGG 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 47, 190
EcoT14I CCWWGG 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 2 cut(s) 589, 617
FaiI YATR 9 cut(s) 182, 205, 207, 314, 371, 572, 574, 587, 615
FatI CATG 2 cut(s) 585, 613
FauI CCCGC 1 cut(s) 133
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 4 cut(s) 6, 36, 87, 249
GluI GCNGC 1 cut(s) 408
HaeIII GGCC 1 cut(s) 52
HapII CCGG 1 cut(s) 235
Hin1II CATG 2 cut(s) 589, 617
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 2 cut(s) 153, 432
HpaII CCGG 1 cut(s) 235
HphI GGTGA 2 cut(s) 196, 401
Hpy166II GTNNAC 3 cut(s) 24, 111, 449
Hpy188I TCNGA 2 cut(s) 79, 292
Hpy188III TCNNGA 2 cut(s) 87, 150
Hpy8I GTNNAC 3 cut(s) 24, 111, 449
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 1 cut(s) 386
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 3 cut(s) 320, 449, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 3 cut(s) 148, 210, 289
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 2 cut(s) 589, 617
Kzo9I GATC 4 cut(s) 74, 225, 280, 468
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 247
MaeI CTAG 4 cut(s) 6, 36, 87, 249
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 344, 543
MalI GATC 4 cut(s) 76, 227, 282, 470
MboI GATC 4 cut(s) 74, 225, 280, 468
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 3 cut(s) 124, 369, 451
MluCI AATT 3 cut(s) 65, 82, 339
MluI ACGCGT 1 cut(s) 451
MlyI GAGTC 1 cut(s) 147
MnlI CCTC 3 cut(s) 241, 319, 544
MseI TTAA 6 cut(s) 69, 165, 420, 441, 594, 625
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 3 cut(s) 49, 192, 235
MvaI CCWGG 2 cut(s) 49, 192
MvnI CGCG 1 cut(s) 453
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 235
NdeII GATC 4 cut(s) 74, 225, 280, 468
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 2 cut(s) 589, 617
NmuCI GTSAC 2 cut(s) 344, 543
PcsI WCGNNNNNNNCGW 1 cut(s) 526
PfeI GAWTC 1 cut(s) 432
PflMI CCANNNNNTGG 1 cut(s) 59
PkrI GCNGC 1 cut(s) 409
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
PpuMI RGGWCCY 1 cut(s) 415
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 47, 190
PspGI CCWGG 2 cut(s) 47, 190
PspPI GGNCC 1 cut(s) 415
PspPPI RGGWCCY 1 cut(s) 415
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 3 cut(s) 112, 361, 622
RsaNI GTAC 3 cut(s) 111, 360, 621
SaqAI TTAA 6 cut(s) 69, 165, 420, 441, 594, 625
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 4 cut(s) 74, 225, 280, 468
Sau96I GGNCC 1 cut(s) 415
ScaI AGTACT 1 cut(s) 622
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 3 cut(s) 49, 192, 235
SduI GDGCHC 3 cut(s) 124, 369, 451
SetI ASST 6 cut(s) 60, 216, 275, 417, 448, 559
SfaNI GCATC 1 cut(s) 247
SinI GGWCC 1 cut(s) 415
Sse9I AATT 3 cut(s) 65, 82, 339
SsiI CCGC 1 cut(s) 126
SspMI CTAG 4 cut(s) 6, 36, 87, 249
StyD4I CCNGG 3 cut(s) 47, 190, 233
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 1 cut(s) 275
TaqI TCGA 2 cut(s) 241, 559
TasI AATT 3 cut(s) 65, 82, 339
TatI WGTACW 2 cut(s) 110, 620
TfiI GAWTC 1 cut(s) 432
Tru1I TTAA 6 cut(s) 69, 165, 420, 441, 594, 625
Tru9I TTAA 6 cut(s) 69, 165, 420, 441, 594, 625
TseFI GTSAC 2 cut(s) 344, 543
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 2 cut(s) 344, 543
TspGWI ACGGA 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 59
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 1 cut(s) 82
XbaI TCTAGA 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 23
XspI CTAG 4 cut(s) 6, 36, 87, 249
ZrmI AGTACT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.