Rh7CG422500

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001715
Physical Location & Seq
Forward (+)
54641289 .. 54643571
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG422500.1

Sequence Viewer

Length: 498 bp
ATGATTTGTCCTTTACAAGCACCTGCATTGAGATTGAGCAGTTTCTCAGGACTCCGTAGTGTTCATGCTTTAGATATTATGACGAGGCCTAGGCAAGACTTATATTCTAATGTGGGAGAAGCTGTCTCCTCTCAGCGTCGAAATGCTTTCAGATGTGTGCCTAAAGCCATGTTTGAGCGCTTCTCAGAAAAGGCAATAAAGGTGATTTTGCTTGCACAAGAGGAAGCAAGGCGACTTGGTCACAACTTCGCTGGTACAGAGCAGATACTTTTGGGTCTTATTGGTGAAGGAACTGGAATTGCTGCCAGGGTCCTTAAATATATGAGAATCAATCTTAAAGGTGCACGCGTTGAAGTGGAAAAGATCATTGGAAGGGGTAGTGGTTTTGTTGCTGTTGAAATTCCGTTCACTCCTCTTGCAAAGCGAGTTTTGGAACTGTCACTGGAGGAAGCTCGTCAACTGGTGATCGCTTTCTACCTGATAAAGCAATTGACTTGA

Protein Analysis

165

Amino Acids

18.37

Weight (kDa)

10.07

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 60 - 154 1.1e-19 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccII CGCG 1 cut(s) 348
AcsI RAATTY 1 cut(s) 399
AfaI GTAC 1 cut(s) 256
AfeI AGCGCT 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 2 cut(s) 353, 398
AjnI CCWGG 1 cut(s) 305
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 2 cut(s) 122, 452
AluI AGCT 2 cut(s) 122, 452
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 1 cut(s) 130
Alw44I GTGCAC 1 cut(s) 342
Aor51HI AGCGCT 1 cut(s) 179
AoxI GGCC 1 cut(s) 86
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 1 cut(s) 399
AspA2I CCTAGG 1 cut(s) 89
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 3 cut(s) 214, 296, 475
AvaII GGWCC 1 cut(s) 310
AvrII CCTAGG 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 346
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 1 cut(s) 90
BfoI RGCGCY 1 cut(s) 181
BfuAI ACCTGC 1 cut(s) 31
BisI GCNGC 1 cut(s) 303
BlnI CCTAGG 1 cut(s) 89
BlsI GCNGC 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 307
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 167, 199
BpmI CTGGAG 1 cut(s) 464
BsaJI CCNNGG 2 cut(s) 89, 306
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bse1I ACTGG 3 cut(s) 298, 447, 465
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 2 cut(s) 89, 306
BseMII CTCAG 3 cut(s) 60, 146, 198
BseNI ACTGG 3 cut(s) 298, 447, 465
BseRI GAGGAG 2 cut(s) 118, 402
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 1 cut(s) 289
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 346
BsmAI GTCTC 1 cut(s) 130
BsnI GGCC 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 2 cut(s) 363, 465
BspANI GGCC 1 cut(s) 88
BspCNI CTCAG 3 cut(s) 59, 145, 197
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 311
BspMI ACCTGC 1 cut(s) 31
BsrI ACTGG 3 cut(s) 298, 447, 465
BssECI CCNNGG 2 cut(s) 89, 306
BssMI GATC 2 cut(s) 363, 465
BssT1I CCWWGG 1 cut(s) 89
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 438
BstC8I GCNNGC 2 cut(s) 213, 346
BstDEI CTNAG 3 cut(s) 46, 132, 184
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 181
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 2 cut(s) 366, 468
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 363, 465
BstNI CCWGG 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 305
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 289
BsuRI GGCC 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 440
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 2 cut(s) 213, 346
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 255
CviAII CATG 2 cut(s) 65, 169
CviJI RGCY 4 cut(s) 88, 122, 167, 452
CviKI_1 RGCY 4 cut(s) 88, 122, 167, 452
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 3 cut(s) 46, 132, 184
DpnI GATC 2 cut(s) 365, 467
DpnII GATC 2 cut(s) 363, 465
Eco130I CCWWGG 1 cut(s) 89
Eco147I AGGCCT 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 310
Eco47III AGCGCT 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 2 cut(s) 68, 172
FaiI YATR 6 cut(s) 66, 80, 103, 170, 321, 323
FatI CATG 2 cut(s) 64, 168
Fnu4HI GCNGC 1 cut(s) 303
Fsp4HI GCNGC 1 cut(s) 303
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 179
GluI GCNGC 1 cut(s) 303
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 181
HaeIII GGCC 1 cut(s) 88
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 180
Hin1II CATG 2 cut(s) 68, 172
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 458
HindII GTYRAC 1 cut(s) 458
HinfI GANTC 2 cut(s) 51, 327
HphI GGTGA 3 cut(s) 214, 296, 475
Hpy166II GTNNAC 3 cut(s) 344, 408, 458
Hpy188I TCNGA 2 cut(s) 152, 187
Hpy188III TCNNGA 1 cut(s) 48
Hpy8I GTNNAC 3 cut(s) 344, 408, 458
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 2 cut(s) 281, 366
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4V TGCA 4 cut(s) 26, 215, 344, 419
HpyF3I CTNAG 3 cut(s) 46, 132, 184
Hsp92II CATG 2 cut(s) 68, 172
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 2 cut(s) 363, 465
LpnPI CCDG 9 cut(s) 33, 36, 237, 279, 292, 319, 428, 446, 491
Lsp1109I GCAGC 1 cut(s) 289
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 239, 438
MalI GATC 2 cut(s) 365, 467
MboI GATC 2 cut(s) 363, 465
MfeI CAATTG 1 cut(s) 488
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 3 cut(s) 297, 399, 488
MluI ACGCGT 1 cut(s) 346
MlyI GAGTC 1 cut(s) 45
MnlI CCTC 5 cut(s) 78, 139, 214, 423, 439
MseI TTAA 2 cut(s) 315, 336
MspR9I CCNGG 1 cut(s) 307
MunI CAATTG 1 cut(s) 488
MvaI CCWGG 1 cut(s) 307
MvnI CGCG 1 cut(s) 348
NdeII GATC 2 cut(s) 363, 465
NlaIII CATG 2 cut(s) 68, 172
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 2 cut(s) 239, 438
PaqCI CACCTGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 88
PfeI GAWTC 1 cut(s) 327
PflFI GACNNNGTC 1 cut(s) 237
PkrI GCNGC 1 cut(s) 304
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
PpuMI RGGWCCY 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 310
PspPPI RGGWCCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 237
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 315, 336
SatI GCNGC 1 cut(s) 303
Sau3AI GATC 2 cut(s) 363, 465
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 1 cut(s) 307
SduI GDGCHC 1 cut(s) 346
SetI ASST 6 cut(s) 25, 124, 204, 343, 454, 480
SinI GGWCC 1 cut(s) 310
Sse9I AATT 3 cut(s) 297, 399, 488
SseBI AGGCCT 1 cut(s) 88
SspMI CTAG 1 cut(s) 90
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 1 cut(s) 305
StyI CCWWGG 1 cut(s) 89
TaaI ACNGT 1 cut(s) 438
TaqI TCGA 1 cut(s) 139
TasI AATT 3 cut(s) 297, 399, 488
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 2 cut(s) 315, 336
Tru9I TTAA 2 cut(s) 315, 336
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 2 cut(s) 239, 438
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 2 cut(s) 239, 438
TspDTI ATGAA 1 cut(s) 53
TspGWI ACGGA 2 cut(s) 44, 393
TspRI CASTG 1 cut(s) 447
Tth111I GACNNNGTC 1 cut(s) 237
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 399
XmaJI CCTAGG 1 cut(s) 89
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.