Rh7AG403200

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001715
Physical Location & Seq
Forward (+)
53286041 .. 53286634
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG403200.1

Sequence Viewer

Length: 594 bp
ATGGCTGGGGTTTCAGTCCAGTTTAGTATTATTCCTAGTGTGGTTGCCGGGCGAAGGGCAACGAAGATGATTTGTCCTTTACAAGCACCTGCATTGAGATTGAGCAGTTTCTCAGGACTCCGTAGTGTTCATGCTTTAGATATTATGACGAGGCCTAGGCAAGACTTATATTCTAATGTGGGAGAAGCTGTCTCCTCTCAGCGTCGAAATGCTTTCAGATGTGTGCCTAAAGCCATGTTTGAGCGCTTCTCAGAAAAGGCAATAAAGGTGATTTTGCTTGCACAAGAGGAAGCAAGGCGACTTGGTCACAACTTCGCTGGTACAGAGCAGATACTTTTGGGTCTTATTGGTGAAGGAACTGGAATTGCTGCCAGGGTCCTTAAATATATGAGAATCAATCTTAAAGGTGCACGCGTTGAAGTGGAAAAGATCATTGGAAGGGGTAGTGGTTTTGTTGCTGTTGAAATTCCGTTCACTCCTCTTGCAAAGCGAGTTTTGGAACTGTCACTGGAGGAAGCTCGTCAACTGCGTATATATTATTTTTCGCTGTTCTTTAAATATCTTTCAGAGAAACATTTATCTGGTTTTAAATAA

Protein Analysis

197

Amino Acids

21.99

Weight (kDa)

10.31

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 82 - 176 1.1e-19 Clp repeat (R) N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 97
Acc36I ACCTGC 1 cut(s) 97
AccII CGCG 1 cut(s) 414
AcsI RAATTY 1 cut(s) 465
AfaI GTAC 1 cut(s) 322
AfeI AGCGCT 1 cut(s) 245
AfiI CCNNNNNNNGG 1 cut(s) 54
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 2 cut(s) 419, 464
AjnI CCWGG 1 cut(s) 371
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
AluBI AGCT 2 cut(s) 188, 518
AluI AGCT 2 cut(s) 188, 518
Alw21I GWGCWC 1 cut(s) 412
Alw26I GTCTC 1 cut(s) 196
Alw44I GTGCAC 1 cut(s) 408
Aor51HI AGCGCT 1 cut(s) 245
AoxI GGCC 1 cut(s) 152
ApaLI GTGCAC 1 cut(s) 408
ApeKI GCWGC 1 cut(s) 368
ApoI RAATTY 1 cut(s) 465
AspA2I CCTAGG 1 cut(s) 155
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 376
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 280, 362
AvaII GGWCC 1 cut(s) 376
AvrII CCTAGG 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 1 cut(s) 355
BciT130I CCWGG 1 cut(s) 373
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 2 cut(s) 36, 156
BfoI RGCGCY 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 1 cut(s) 369
BlnI CCTAGG 1 cut(s) 155
BlsI GCNGC 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 49, 373
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 377
BmrFI CCNGG 2 cut(s) 49, 373
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 155, 372
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 54
Bse1I ACTGG 3 cut(s) 19, 364, 513
BseBI CCWGG 1 cut(s) 373
BseDI CCNNGG 2 cut(s) 155, 372
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 126, 212, 264
BseNI ACTGG 3 cut(s) 19, 364, 513
BseRI GAGGAG 2 cut(s) 184, 468
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 1 cut(s) 355
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 196
BsnI GGCC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 412
Bsp143I GATC 1 cut(s) 429
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 3 cut(s) 125, 211, 263
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 377
BspMI ACCTGC 1 cut(s) 97
BsrI ACTGG 3 cut(s) 19, 364, 513
BssECI CCNNGG 2 cut(s) 155, 372
BssMI GATC 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 504
BstC8I GCNNGC 2 cut(s) 279, 412
BstDEI CTNAG 3 cut(s) 112, 198, 250
BstFNI CGCG 1 cut(s) 414
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 1 cut(s) 432
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 1 cut(s) 429
BstNI CCWGG 1 cut(s) 373
BstSCI CCNGG 2 cut(s) 47, 371
BstSLI GKGCMC 1 cut(s) 412
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 355
BsuRI GGCC 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 506
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 2 cut(s) 279, 412
CfoI GCGC 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 376
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 321
CviAII CATG 2 cut(s) 131, 235
CviJI RGCY 5 cut(s) 5, 154, 188, 233, 518
CviKI_1 RGCY 5 cut(s) 5, 154, 188, 233, 518
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 3 cut(s) 112, 198, 250
DpnI GATC 1 cut(s) 431
DpnII GATC 1 cut(s) 429
DraI TTTAAA 2 cut(s) 556, 589
Eco130I CCWWGG 1 cut(s) 155
Eco147I AGGCCT 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 376
Eco47III AGCGCT 1 cut(s) 245
EcoO109I RGGNCCY 1 cut(s) 376
EcoRII CCWGG 1 cut(s) 371
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 2 cut(s) 134, 238
FaiI YATR 8 cut(s) 132, 146, 169, 236, 387, 389, 533, 535
FatI CATG 2 cut(s) 130, 234
Fnu4HI GCNGC 1 cut(s) 369
Fsp4HI GCNGC 1 cut(s) 369
FspBI CTAG 2 cut(s) 36, 156
GlaI GCGC 1 cut(s) 245
GluI GCNGC 1 cut(s) 369
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 1 cut(s) 154
HapII CCGG 1 cut(s) 48
HgaI GACGC 1 cut(s) 191
HhaI GCGC 1 cut(s) 246
Hin1II CATG 2 cut(s) 134, 238
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HinfI GANTC 2 cut(s) 117, 393
HpaII CCGG 1 cut(s) 48
HphI GGTGA 2 cut(s) 280, 362
Hpy166II GTNNAC 3 cut(s) 410, 474, 524
Hpy188I TCNGA 3 cut(s) 218, 253, 568
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 3 cut(s) 410, 474, 524
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 3 cut(s) 48, 347, 432
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4V TGCA 4 cut(s) 92, 281, 410, 485
HpyF3I CTNAG 3 cut(s) 112, 198, 250
Hsp92II CATG 2 cut(s) 134, 238
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 429
Lsp1109I GCAGC 1 cut(s) 355
MaeI CTAG 2 cut(s) 36, 156
MaeIII GTNAC 2 cut(s) 305, 504
MalI GATC 1 cut(s) 431
MboI GATC 1 cut(s) 429
MboII GAAGA 1 cut(s) 76
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 2 cut(s) 363, 465
MluI ACGCGT 1 cut(s) 412
MlyI GAGTC 1 cut(s) 111
MnlI CCTC 5 cut(s) 144, 205, 280, 489, 505
MseI TTAA 4 cut(s) 381, 402, 555, 588
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 49, 373
MvaI CCWGG 1 cut(s) 373
MvnI CGCG 1 cut(s) 414
NciI CCSGG 1 cut(s) 49
NdeII GATC 1 cut(s) 429
NlaIII CATG 2 cut(s) 134, 238
NlaIV GGNNCC 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 305, 504
PaqCI CACCTGC 1 cut(s) 97
PceI AGGCCT 1 cut(s) 154
PcsI WCGNNNNNNNCGW 1 cut(s) 526
PfeI GAWTC 1 cut(s) 393
PflFI GACNNNGTC 1 cut(s) 303
PkrI GCNGC 1 cut(s) 370
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 376
Psp5II RGGWCCY 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 377
PspPI GGNCC 1 cut(s) 376
PspPPI RGGWCCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 303
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
SaqAI TTAA 4 cut(s) 381, 402, 555, 588
SatI GCNGC 1 cut(s) 369
Sau3AI GATC 1 cut(s) 429
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 2 cut(s) 49, 373
SduI GDGCHC 1 cut(s) 412
SetI ASST 5 cut(s) 91, 190, 270, 409, 520
SinI GGWCC 1 cut(s) 376
Sse9I AATT 2 cut(s) 363, 465
SseBI AGGCCT 1 cut(s) 154
SspMI CTAG 2 cut(s) 36, 156
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 47, 371
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 504
TaqI TCGA 1 cut(s) 205
TasI AATT 2 cut(s) 363, 465
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 4 cut(s) 381, 402, 555, 588
Tru9I TTAA 4 cut(s) 381, 402, 555, 588
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 2 cut(s) 305, 504
TseI GCWGC 1 cut(s) 368
Tsp45I GTSAC 2 cut(s) 305, 504
TspDTI ATGAA 1 cut(s) 119
TspGWI ACGGA 2 cut(s) 110, 459
TspRI CASTG 1 cut(s) 513
Tth111I GACNNNGTC 1 cut(s) 303
VneI GTGCAC 1 cut(s) 408
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 1 cut(s) 465
XmaJI CCTAGG 1 cut(s) 155
XspI CTAG 2 cut(s) 36, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.