Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001715

Physical Location & Seq

Genomic Coordinates Forward (+)

29021664 .. 29028748

Gene Structure 7085 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018383

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1677 bp

ATGTCAGAGCCAATCATCCTCTCAGACGAAGAAGAAGAGCACCACAATGCCTCATTCCCACCATTTCCCTCCAAGAAACGCCGAACCCAATTCGAACCCAATCCGAATCCGAATCCGAATCCAAATCCTACGGTTCTCATCCTCGACGACGACCCGACGCCGCAGAAACCCGGCCCCAAATCCACGGCCTACTTGGTCCCGGACACCCCGAATTCCGATATCGCAATCGTGAAATGCACAAGGGCTGCCCAGCACAAGTTATCTGGCATCAATGGATTGATATGTTTGGAATCCGATAATGAGCCCGGAAGTAGTTGTGGAGGGGAAAAGAAGAAGGAAAATGGATCAATGCCGGGTGGTTTTGGTCTGGCCAAGGTCTTGGATTGGAGTCCGAGTTTTGTTGAGTCCTCATCTGCACTTGGACATTCTGAGCTGGCGCGCACTCTTGAGGGCAGCTCTTCAAATCCTACTTCTTTGCAGGATGATATTGATCAGGTACTTGATTGTCCTGACGATGAAAACACAATGGATCAAATGGGCAACATTATGAGACCAAGAAAAGAATCTATAGCTATTACAGAAAAGGAAAATATCACTAAAGAAGTAAAGGGAAGAAAGAAGATGACCAAAGAGGAAAGGACACGCTTAATGGAAGAAAAGAAGCTAAAGAAGCTTGAAGAGAAGTTGCAAAGAGAGGCTCTGAAAGCTGAAGCTGCAGAGATGAAAAAATTACAGAAAGAAAAGCTAAGGTGGGAAAAAGGGAAGTTCGCACTTAAATCCATTGTGGCCGAGATTGATTCTAAAGTAATTGAATCCGGATCAGTTGGAGGAAGTCTGCTTACAAGGTTTGCCGAAAGGGGGTTAACATACCGTATAACATCAAATCCAATTGAAAGATCAATTGTCTGGACGATGACTGTTCCGGAACATATTTCACAGCTTTCTCCTGGAGGAGTGGAGATTCAATATATATTGATTGTTTATGAGGCTGAAGATTTTTGTAAACTCATCATCAACGAATCTCTTCCGGGACATGTCTTGAGAGTTCAAAACCATTATCCATCATATACAGTGTGTTACCTCACAAATAAGTTGATTGCATATGTTAGGAAAAGGGAAGGACAATTGTACAATGGCCGGGGCTGTGAGGGACACCCAAGTGTTGACAAGGTTGAAAAGGTGTTGGCAAATTTGACCACTAGCTTTTCCAAAGTACATTCCAGGCAGTGCAAAGATGAGGCTGAACTTGCTGAACATGTTGTTGGTTTAACATGTAGCCTGTCATCCTGCCTTTTCAGGAAGAAGTTAACTAGACTTGATGTAAATGCTAATGGATCCTTTATCACAAAGGACTGCGTTGACCGGCATTTGATAAAAAAGAGCTCATGGTTAAAGGCTTTGGTAGCTATCCCGAAGGTGCAGCCACGATTTGCTATTGCCATAGGGAAGAAGTATCCTACCTTGAAGTCTCTGTTGAGTGTTTACATGGACCCAAATATATCAGTACATGAAAAGGAATTTCTGCTCAAGGACTTGACAACAGAAGGTTTACTCGGTGGTGATAGAAGATTAGGTGAGGTGTGTTCCAAGAGAGTGTACAGAATACTCATGGCACAAAGTGGCTGCACCAAGACCGATGATGTTGAGGATGGTGCAGATTTTTTCAGGGTCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0006139 GO:0006259 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009987 GO:0010212 GO:0010332 GO:0016787 GO:0016788 GO:0033554 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0090305 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

ERCC4

Protein Analysis

559

Amino Acids

62.29

Weight (kDa)

8.09

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ERCC4	PF02732	265 - 421	1.9e-14	ERCC4 domain
EME1-MUS81_C	PF21292	463 - 533	8.8e-08	EME1/MUS81, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001715)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21800 AT2G21800 AT2G21800 AT2G22140 AT2G22140
fragaria_vesca	FvH4_1g23210 FvH4_1g23210 FvH4_1g23210 FvH4_1g23210 FvH4_1g23210 FvH4_1g23210 FvH4_5g31350 FvH4_5g31350
malus_domestica	MD15G1335700.v1.1 MD15G1404100.v1.1
prunus_persica	Prupe.1G550600_v2.0.a1 Prupe.1G550600_v2.0.a1 Prupe.1G550600_v2.0.a1 Prupe.6G166800_v2.0.a1
pyrus_communis	pycom15g28970
rosa_chinensis	RchiOBHm_Chr2g0117911 RchiOBHm_Chr7g0226601 RchiOBHm_Chr7g0226611 RchiOBHm_Chr7g0228631
rosa_laevigata	RLG00000001714 RLG00000018383
rosa_multiflora	Rmu_sc0001423.1_g000001
rosa_roxburghii	Rroxscaffold_2G00126110 Rroxscaffold_3G00230990 Rroxscaffold_3G00232670
rosa_rugosa	Rorug02G0214800 Rorug02G0214900 Rorug02G0214900 Rorug07G0238500 Rorug07G0238600
rosa_samantha	Rh2AG271200 Rh2BG282800 Rh2DG279100 Rh2DG297000 Rh7AG386100 Rh7AG403200 Rh7BG372700 Rh7BG372800 Rh7CG405400 Rh7CG422500 Rh7DG384700
rosa_wichuraiana	Rw2G021450 Rw7G032410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	439
AccIII	TCCGGA	2 cut(s)	815, 922
AciI	CCGC	1 cut(s)	161
AclWI	GGATC	5 cut(s)	352, 537, 826, 1329, 1342
AcoI	YGGCCR	3 cut(s)	369, 786, 1135
AcsI	RAATTY	3 cut(s)	211, 1189, 1517
AcuI	CTGAAG	2 cut(s)	729, 1011
AcyI	GRCGYC	1 cut(s)	158
AdeI	CACNNNGTG	1 cut(s)	1619
AfaI	GTAC	5 cut(s)	498, 1130, 1215, 1506, 1598
AfiI	CCNNNNNNNGG	1 cut(s)	858
AflIII	ACRYGT	3 cut(s)	1033, 1255, 1271
AgsI	TTSAA	8 cut(s)	462, 677, 812, 893, 965, 1049, 1175, 1465
AjnI	CCWGG	2 cut(s)	946, 1220
AjuI	GAANNNNNNNTTGG	2 cut(s)	1245, 1277
AleI	CACNNNNGTG	1 cut(s)	1158
Alw21I	GWGCWC	2 cut(s)	42, 1385
Alw26I	GTCTC	2 cut(s)	544, 1473
AlwI	GGATC	5 cut(s)	352, 537, 826, 1329, 1342
Aor13HI	TCCGGA	2 cut(s)	815, 922
AoxI	GGCC	5 cut(s)	172, 186, 369, 786, 1135
ApeKI	GCWGC	5 cut(s)	245, 453, 713, 1420, 1623
ApoI	RAATTY	3 cut(s)	211, 1189, 1517
Asp700I	GAANNNNTTC	1 cut(s)	1023
AspLEI	GCGC	2 cut(s)	439, 441
AspS9I	GGNCC	3 cut(s)	173, 196, 1489
AsuC2I	CCSGG	6 cut(s)	171, 200, 306, 354, 1029, 1139
AsuHPI	GGTGA	2 cut(s)	1571, 1586
AsuII	TTCGAA	1 cut(s)	93
AvaII	GGWCC	2 cut(s)	196, 1489
BalI	TGGCCA	1 cut(s)	371
BamHI	GGATCC	1 cut(s)	1334
BanII	GRGCYC	2 cut(s)	306, 1385
BarI	GAAGNNNNNNTAC	2 cut(s)	823, 855
Bbv12I	GWGCWC	2 cut(s)	42, 1385
BbvI	GCAGC	5 cut(s)	232, 465, 700, 1432, 1610
BccI	CCATC	2 cut(s)	1069, 1643
BceAI	ACGGC	1 cut(s)	201
BciT130I	CCWGG	2 cut(s)	948, 1222
BciVI	GTATCC	1 cut(s)	1464
BclI	TGATCA	1 cut(s)	490
BcnI	CCSGG	6 cut(s)	171, 200, 306, 354, 1029, 1139
BcoDI	GTCTC	2 cut(s)	544, 1473
BfaI	CTAG	2 cut(s)	1200, 1311
BfmI	CTRYAG	2 cut(s)	567, 714
BfuI	GTATCC	1 cut(s)	1464
BisI	GCNGC	6 cut(s)	161, 246, 454, 714, 1421, 1624
BlsI	GCNGC	6 cut(s)	162, 247, 455, 715, 1422, 1625
Bme1390I	CCNGG	8 cut(s)	171, 200, 306, 354, 948, 1029, 1139, 1222
Bme18I	GGWCC	2 cut(s)	196, 1489
BmgT120I	GGNCC	3 cut(s)	173, 196, 1489
BmiI	GGNNCC	4 cut(s)	175, 198, 1336, 1491
BmrFI	CCNGG	8 cut(s)	171, 200, 306, 354, 948, 1029, 1139, 1222
BmsI	GCATC	1 cut(s)	276
BplI	GAGNNNNNCTC	2 cut(s)	440, 472
BpmI	CTGGAG	1 cut(s)	969
Bpu10I	CCTNAGC	1 cut(s)	746
Bpu14I	TTCGAA	1 cut(s)	93
BpuEI	CTTGAG	3 cut(s)	467, 1060, 1511
BpuMI	CCSGG	6 cut(s)	171, 200, 306, 354, 1029, 1139
BsaBI	GATNNNNATC	1 cut(s)	489
BsaHI	GRCGYC	1 cut(s)	158
BsaI	GGTCTC	1 cut(s)	544
BsaJI	CCNNGG	3 cut(s)	183, 372, 1138
BsaWI	WCCGGW	2 cut(s)	815, 922
BsaXI	ACNNNNNCTCC	2 cut(s)	379, 409
Bsc4I	CCNNNNNNNGG	1 cut(s)	858
Bse118I	RCCGGY	1 cut(s)	1362
Bse8I	GATNNNNATC	1 cut(s)	489
BseAI	TCCGGA	2 cut(s)	815, 922
BseBI	CCWGG	2 cut(s)	948, 1222
BseDI	CCNNGG	3 cut(s)	183, 372, 1138
BseGI	GGATG	5 cut(s)	15, 138, 487, 1283, 1654
BseJI	GATNNNNATC	1 cut(s)	489
BseLI	CCNNNNNNNGG	1 cut(s)	858
BseMII	CTCAG	2 cut(s)	36, 420
BsePI	GCGCGC	1 cut(s)	437
BseRI	GAGGAG	1 cut(s)	966
BseXI	GCAGC	5 cut(s)	232, 465, 700, 1432, 1610
BseYI	CCCAGC	1 cut(s)	249
BsgI	GTGCAG	4 cut(s)	399, 1439, 1609, 1674
Bsh1236I	CGCG	1 cut(s)	439
BshFI	GGCC	5 cut(s)	174, 188, 371, 788, 1137
BsiHKAI	GWGCWC	2 cut(s)	42, 1385
BsiSI	CCGG	9 cut(s)	171, 200, 306, 353, 816, 923, 1028, 1138, 1363
BslFI	GGGAC	3 cut(s)	182, 1044, 1164
BslI	CCNNNNNNNGG	1 cut(s)	858
BsmAI	GTCTC	2 cut(s)	544, 1473
BsmFI	GGGAC	3 cut(s)	182, 1044, 1164
BsnI	GGCC	5 cut(s)	174, 188, 371, 788, 1137
Bso31I	GGTCTC	1 cut(s)	544
Bsp119I	TTCGAA	1 cut(s)	93
Bsp1286I	GDGCHC	3 cut(s)	42, 306, 1385
Bsp13I	TCCGGA	2 cut(s)	815, 922
Bsp1407I	TGTACA	2 cut(s)	1128, 1596
Bsp143I	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
BspACI	CCGC	1 cut(s)	161
BspANI	GGCC	5 cut(s)	174, 188, 371, 788, 1137
BspCNI	CTCAG	2 cut(s)	35, 421
BspEI	TCCGGA	2 cut(s)	815, 922
BspFNI	CGCG	1 cut(s)	439
BspLI	GGNNCC	4 cut(s)	175, 198, 1336, 1491
BspMAI	CTGCAG	1 cut(s)	718
BspPI	GGATC	5 cut(s)	352, 537, 826, 1329, 1342
BspQI	GCTCTTC	2 cut(s)	30, 463
BspT104I	TTCGAA	1 cut(s)	93
BspTNI	GGTCTC	1 cut(s)	544
BsrFI	RCCGGY	1 cut(s)	1362
BsrGI	TGTACA	2 cut(s)	1128, 1596
BssAI	RCCGGY	1 cut(s)	1362
BssECI	CCNNGG	3 cut(s)	183, 372, 1138
BssHII	GCGCGC	1 cut(s)	437
BssMI	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
BssNI	GRCGYC	1 cut(s)	158
BssT1I	CCWWGG	1 cut(s)	372
Bst2UI	CCWGG	2 cut(s)	948, 1222
Bst4CI	ACNGT	4 cut(s)	133, 872, 919, 1072
Bst6I	CTCTTC	4 cut(s)	30, 463, 672, 1029
BstACI	GRCGYC	1 cut(s)	158
BstAUI	TGTACA	2 cut(s)	1128, 1596
BstBI	TTCGAA	1 cut(s)	93
BstC8I	GCNNGC	2 cut(s)	435, 439
BstDEI	CTNAG	3 cut(s)	22, 429, 746
BstDSI	CCRYGG	1 cut(s)	183
BstF5I	GGATG	5 cut(s)	15, 138, 487, 1283, 1654
BstFNI	CGCG	1 cut(s)	439
BstHHI	GCGC	2 cut(s)	439, 441
BstKTI	GATC	6 cut(s)	347, 493, 532, 821, 899, 1337
BstMAI	GTCTC	2 cut(s)	544, 1473
BstMBI	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
BstMWI	GCNNNNNNNGC	5 cut(s)	670, 704, 713, 1247, 1403
BstNI	CCWGG	2 cut(s)	948, 1222
BstNSI	RCATGY	3 cut(s)	1037, 1259, 1275
BstSCI	CCNGG	8 cut(s)	169, 198, 304, 352, 946, 1027, 1137, 1220
BstSFI	CTRYAG	2 cut(s)	567, 714
BstUI	CGCG	1 cut(s)	439
BstV1I	GCAGC	5 cut(s)	232, 465, 700, 1432, 1610
BstX2I	RGATCY	1 cut(s)	1334
BstXI	CCANNNNNNTGG	1 cut(s)	379
BstYI	RGATCY	1 cut(s)	1334
BsuI	GTATCC	1 cut(s)	1464
BsuRI	GGCC	5 cut(s)	174, 188, 371, 788, 1137
BtgI	CCRYGG	1 cut(s)	183
BtsCI	GGATG	5 cut(s)	15, 138, 487, 1283, 1654
BtsI	GCAGTG	1 cut(s)	1232
BtsIMutI	CAGTG	2 cut(s)	1077, 1232
Cac8I	GCNNGC	2 cut(s)	435, 439
CfoI	GCGC	2 cut(s)	439, 441
Cfr10I	RCCGGY	1 cut(s)	1362
Cfr13I	GGNCC	3 cut(s)	173, 196, 1489
CseI	GACGC	1 cut(s)	166
Csp6I	GTAC	5 cut(s)	497, 1129, 1214, 1505, 1597
CviAII	CATG	7 cut(s)	1034, 1256, 1272, 1386, 1486, 1508, 1609
CviQI	GTAC	5 cut(s)	497, 1129, 1214, 1505, 1597
DdeI	CTNAG	3 cut(s)	22, 429, 746
DpnI	GATC	6 cut(s)	346, 492, 531, 820, 898, 1336
DpnII	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
DraIII	CACNNNGTG	1 cut(s)	1619
EaeI	YGGCCR	3 cut(s)	369, 786, 1135
Eam1104I	CTCTTC	4 cut(s)	30, 463, 672, 1029
EarI	CTCTTC	4 cut(s)	30, 463, 672, 1029
Ecl136II	GAGCTC	1 cut(s)	1383
Eco130I	CCWWGG	1 cut(s)	372
Eco24I	GRGCYC	2 cut(s)	306, 1385
Eco31I	GGTCTC	1 cut(s)	544
Eco32I	GATATC	1 cut(s)	220
Eco47I	GGWCC	2 cut(s)	196, 1489
Eco53kI	GAGCTC	1 cut(s)	1383
Eco57I	CTGAAG	2 cut(s)	729, 1011
EcoICRI	GAGCTC	1 cut(s)	1383
EcoRI	GAATTC	1 cut(s)	211
EcoRII	CCWGG	2 cut(s)	946, 1220
EcoRV	GATATC	1 cut(s)	220
EcoT14I	CCWWGG	1 cut(s)	372
EcoT38I	GRGCYC	2 cut(s)	306, 1385
ErhI	CCWWGG	1 cut(s)	372
FaeI	CATG	7 cut(s)	1037, 1259, 1275, 1389, 1489, 1511, 1612
FaqI	GGGAC	3 cut(s)	182, 1044, 1164
FatI	CATG	7 cut(s)	1033, 1255, 1271, 1385, 1485, 1507, 1608
FauNDI	CATATG	1 cut(s)	1102
FbaI	TGATCA	1 cut(s)	490
Fnu4HI	GCNGC	6 cut(s)	161, 246, 454, 714, 1421, 1624
FokI	GGATG	5 cut(s)	2, 125, 494, 1270, 1661
FriOI	GRGCYC	2 cut(s)	306, 1385
Fsp4HI	GCNGC	6 cut(s)	161, 246, 454, 714, 1421, 1624
FspBI	CTAG	2 cut(s)	1200, 1311
GlaI	GCGC	2 cut(s)	438, 440
GluI	GCNGC	6 cut(s)	161, 246, 454, 714, 1421, 1624
GsaI	CCCAGC	1 cut(s)	253
GsuI	CTGGAG	1 cut(s)	969
HaeIII	GGCC	5 cut(s)	174, 188, 371, 788, 1137
HapII	CCGG	9 cut(s)	171, 200, 306, 353, 816, 923, 1028, 1138, 1363
HgaI	GACGC	1 cut(s)	166
HhaI	GCGC	2 cut(s)	439, 441
Hin1I	GRCGYC	1 cut(s)	158
Hin1II	CATG	7 cut(s)	1037, 1259, 1275, 1389, 1489, 1511, 1612
Hin6I	GCGC	2 cut(s)	437, 439
HinP1I	GCGC	2 cut(s)	437, 439
HincII	GTYRAC	4 cut(s)	864, 1165, 1308, 1360
HindII	GTYRAC	4 cut(s)	864, 1165, 1308, 1360
HindIII	AAGCTT	1 cut(s)	671
HpaI	GTTAAC	2 cut(s)	864, 1308
HpaII	CCGG	9 cut(s)	171, 200, 306, 353, 816, 923, 1028, 1138, 1363
HphI	GGTGA	2 cut(s)	1571, 1586
Hpy166II	GTNNAC	8 cut(s)	864, 1004, 1165, 1308, 1360, 1483, 1550, 1597
Hpy188III	TCNNGA	9 cut(s)	229, 446, 509, 816, 907, 923, 1039, 1297, 1411
Hpy8I	GTNNAC	8 cut(s)	864, 1004, 1165, 1308, 1360, 1483, 1550, 1597
Hpy99I	CGWCG	3 cut(s)	149, 152, 160
HpyAV	CCTTC	4 cut(s)	328, 1112, 1408, 1538
HpyCH4III	ACNGT	4 cut(s)	133, 872, 919, 1072
HpyF10VI	GCNNNNNNNGC	5 cut(s)	670, 704, 713, 1247, 1403
HpyF3I	CTNAG	3 cut(s)	22, 429, 746
Hsp92I	GRCGYC	1 cut(s)	158
Hsp92II	CATG	7 cut(s)	1037, 1259, 1275, 1389, 1489, 1511, 1612
HspAI	GCGC	2 cut(s)	437, 439
Kpn2I	TCCGGA	2 cut(s)	815, 922
Ksp22I	TGATCA	1 cut(s)	490
KspAI	GTTAAC	2 cut(s)	864, 1308
Kzo9I	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
LguI	GCTCTTC	2 cut(s)	30, 463
Lsp1109I	GCAGC	5 cut(s)	232, 465, 700, 1432, 1610
LweI	GCATC	1 cut(s)	276
MaeI	CTAG	2 cut(s)	1200, 1311
MaeIII	GTNAC	1 cut(s)	1076
MalI	GATC	6 cut(s)	346, 492, 531, 820, 898, 1336
MboI	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
MfeI	CAATTG	4 cut(s)	888, 900, 1124, 1672
MflI	RGATCY	1 cut(s)	1334
MhlI	GDGCHC	3 cut(s)	42, 306, 1385
MlsI	TGGCCA	1 cut(s)	371
MluNI	TGGCCA	1 cut(s)	371
MlyI	GAGTC	2 cut(s)	397, 413
MmeI	TCCRAC	1 cut(s)	805
Mox20I	TGGCCA	1 cut(s)	371
MroI	TCCGGA	2 cut(s)	815, 922
MroXI	GAANNNNTTC	1 cut(s)	1023
MscI	TGGCCA	1 cut(s)	371
MseI	TTAA	6 cut(s)	647, 774, 863, 1268, 1307, 1391
MslI	CAYNNNNRTG	2 cut(s)	45, 1158
Msp20I	TGGCCA	1 cut(s)	371
MspI	CCGG	9 cut(s)	171, 200, 306, 353, 816, 923, 1028, 1138, 1363
MspR9I	CCNGG	8 cut(s)	171, 200, 306, 354, 948, 1029, 1139, 1222
MunI	CAATTG	4 cut(s)	888, 900, 1124, 1672
MvaI	CCWGG	2 cut(s)	948, 1222
MvnI	CGCG	1 cut(s)	439
MwoI	GCNNNNNNNGC	5 cut(s)	670, 704, 713, 1247, 1403
NciI	CCSGG	6 cut(s)	171, 200, 306, 354, 1029, 1139
NdeI	CATATG	1 cut(s)	1102
NdeII	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
NlaIII	CATG	7 cut(s)	1037, 1259, 1275, 1389, 1489, 1511, 1612
NlaIV	GGNNCC	4 cut(s)	175, 198, 1336, 1491
NmeAIII	GCCGAG	1 cut(s)	814
NspI	RCATGY	3 cut(s)	1037, 1259, 1275
NspV	TTCGAA	1 cut(s)	93
OliI	CACNNNNGTG	1 cut(s)	1158
PauI	GCGCGC	1 cut(s)	437
PciI	ACATGT	3 cut(s)	1033, 1255, 1271
PciSI	GCTCTTC	2 cut(s)	30, 463
PdmI	GAANNNNTTC	1 cut(s)	1023
PfeI	GAWTC	9 cut(s)	106, 112, 118, 290, 563, 797, 812, 961, 1019
PfoI	TCCNGGA	3 cut(s)	198, 946, 1027
PkrI	GCNGC	6 cut(s)	162, 247, 455, 715, 1422, 1625
PleI	GAGTC	2 cut(s)	396, 412
PpsI	GAGTC	2 cut(s)	396, 412
PscI	ACATGT	3 cut(s)	1033, 1255, 1271
Psp124BI	GAGCTC	1 cut(s)	1385
Psp6I	CCWGG	2 cut(s)	946, 1220
PspFI	CCCAGC	1 cut(s)	249
PspGI	CCWGG	2 cut(s)	946, 1220
PspN4I	GGNNCC	4 cut(s)	175, 198, 1336, 1491
PspPI	GGNCC	3 cut(s)	173, 196, 1489
PstI	CTGCAG	1 cut(s)	718
PsuI	RGATCY	1 cut(s)	1334
PteI	GCGCGC	1 cut(s)	437
RsaI	GTAC	5 cut(s)	498, 1130, 1215, 1506, 1598
RsaNI	GTAC	5 cut(s)	497, 1129, 1214, 1505, 1597
RseI	CAYNNNNRTG	2 cut(s)	45, 1158
SacI	GAGCTC	1 cut(s)	1385
SapI	GCTCTTC	2 cut(s)	30, 463
SaqAI	TTAA	6 cut(s)	647, 774, 863, 1268, 1307, 1391
SatI	GCNGC	6 cut(s)	161, 246, 454, 714, 1421, 1624
Sau3AI	GATC	6 cut(s)	344, 490, 529, 818, 896, 1334
Sau96I	GGNCC	3 cut(s)	173, 196, 1489
SchI	GAGTC	2 cut(s)	397, 413
ScrFI	CCNGG	8 cut(s)	171, 200, 306, 354, 948, 1029, 1139, 1222
SduI	GDGCHC	3 cut(s)	42, 306, 1385
SfaNI	GCATC	1 cut(s)	276
SfcI	CTRYAG	2 cut(s)	567, 714
SfuI	TTCGAA	1 cut(s)	93
SinI	GGWCC	2 cut(s)	196, 1489
SmiMI	CAYNNNNRTG	2 cut(s)	45, 1158
SmlI	CTYRAG	3 cut(s)	446, 1039, 1526
SmoI	CTYRAG	3 cut(s)	446, 1039, 1526
SsiI	CCGC	1 cut(s)	161
SspMI	CTAG	2 cut(s)	1200, 1311
SstI	GAGCTC	1 cut(s)	1385
StyD4I	CCNGG	8 cut(s)	169, 198, 304, 352, 946, 1027, 1137, 1220
StyI	CCWWGG	1 cut(s)	372
TaaI	ACNGT	4 cut(s)	133, 872, 919, 1072
TaqI	TCGA	2 cut(s)	93, 144
TaqII	GACCGA	1 cut(s)	1649
TatI	WGTACW	4 cut(s)	1128, 1213, 1504, 1596
TauI	GCSGC	1 cut(s)	163
TfiI	GAWTC	9 cut(s)	106, 112, 118, 290, 563, 797, 812, 961, 1019
Tru1I	TTAA	6 cut(s)	647, 774, 863, 1268, 1307, 1391
Tru9I	TTAA	6 cut(s)	647, 774, 863, 1268, 1307, 1391
TscAI	CASTG	2 cut(s)	1077, 1232
TseI	GCWGC	5 cut(s)	245, 453, 713, 1420, 1623
TspDTI	ATGAA	3 cut(s)	531, 737, 1524
TspRI	CASTG	2 cut(s)	1077, 1232
VpaK11BI	GGWCC	2 cut(s)	196, 1489
XapI	RAATTY	3 cut(s)	211, 1189, 1517
XceI	RCATGY	3 cut(s)	1037, 1259, 1275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	190
XmnI	GAANNNNTTC	1 cut(s)	1023
XspI	CTAG	2 cut(s)	1200, 1311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.