Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0006944

Physical Location & Seq

Genomic Coordinates Reverse (-)

10170525 .. 10173106

Gene Structure 2582 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027270

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGGATTCAACTAAATATGGGCTCAGTATCTGTGATGGTAACATTCCTTGCTTCCCAGTTTTGAGCTCTACTGCAAAGAACTTGGCTTCACATGGAACTAGAAGCGCTGAGAGTCCGCCTCACCTAGGAAACCAACACTACCAGAGGAAGGGAAATGCACCACAATCTCCTGAAGATACTTCAGAAGATAATAATCTCCTGGAAAAGCTAATTGGGATGCCAATCCGTTACAGTTACAAGGATCTTAAAACTGCAACTAACAACTTCTCAAATAAGCTTGGGGAAGGAGGTTTTGCCTCAGTTTACTTGGGGGTTCTCCCAGATGGAACTCGACTTGCTGTGAAGAAGTTGGAAGCCAATAAATATCGGGGAAAGAAAGATTTTGAAGCTGAAGTTACCATCAGTGGCAGTATCCATCATCTGCACTTGGTCAGGCTCCGGGGCTTCTGTGCAAAGGGAAGCAATCGACTTCTTGCCTACGAGTACATGGCAAATGGATCCCTGGGCAAATGGATTTTCAGGGAAAAAAATGAAGAGTTCGTATTGGATTGGGGGACAAGATTCAACATAGCAGTGGGTATTGCCAAAGGAATAGCTTATCTCCATGAAGATTGTGATTTGAAGATTATTCACTGTGACATAAATCCTCATAACGTGCTCCTTGACGACAATTACAATGCCAAACTCTCAGATTTTGGTTTGGCAGTGCTGATGACCAGGGAGCAGAGCCATCTCCTCCAAACAGCACGACGAGGAGCTATGGAATACATGGCCCCAGAGTGGTTCTATACAAATCTTGGTGATGTTTCGGAGAAAATTGACGTGTACAGCTACGGAATGCTGCTGCTGGAGATCATTGGTGGAAGAAAGAACTTGGACGCATGTCACTATATCTCTGATGAAATTTATTTTCCATCGTATGCCTCCAAAATGTTGGAAGAAGGGAAACTCAAGGACATCTTCGACTCCAAGATGAGGATAGATGACGTTGACGGGAGGGTTTCTACTGCGGTTATGGTAGCATTGTGGTGTATACAGTTAGACATGACTTTGAGGCCACCAATGACCAAGGTTGTCCAAATGCTTCAAGGCATCTGCTCTGTTCCTCAGCCTCCAATTCTGCAGTTTCCTCCAAATGAATTACCCAAGGGGGGGACTTTCTCGGGTCCATCGGACTGCAACAGCGATGCCTACCTTTCTGCAGAGAGCGCCTCTCCGGGCCAAGATAACATAATTAACATTTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0031625 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044464 GO:0046777 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

B_lectin PAN_2 Pkinase Pkinase_Tyr

Protein Analysis

416

Amino Acids

46.16

Weight (kDa)

5.91

Isoelectric Point (pI)

46.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	89 - 362	6.1e-39	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	90 - 360	3e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006944)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30110
malus_domestica	MD15G1122900.v1.1
rosa_chinensis	RchiOBHm_Chr1g0367661 RchiOBHm_Chr6g0297951
rosa_laevigata	RLG00000011549 RLG00000027270 RLG00000027276
rosa_multiflora	Rmu_co8343541.1_g000001 Rmu_sc0001091.1_g000014 Rmu_sc0008470.1_g000008 Rmu_sc0009775.1_g000007 Rmu_sc0027455.1_g000002
rosa_roxburghii	Rroxscaffold_4G00289420 Rroxscaffold_5G00333540 Rroxscaffold_5G00333550
rosa_rugosa	Rorug01G0336000 Rorug01G0336100 Rorug03G0303500 Rorug06G0277000
rosa_samantha	Rh1AG343100 Rh1BG304200 Rh1DG336500 Rh4BG005400 Rh6BG395800 Rh6BG396000
rosa_wichuraiana	Rw1G030530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1033
AciI	CCGC	2 cut(s)	116, 1010
AclWI	GGATC	3 cut(s)	249, 492, 505
AcsI	RAATTY	1 cut(s)	903
AcuI	CTGAAG	3 cut(s)	165, 192, 411
AfaI	GTAC	2 cut(s)	485, 827
AfeI	AGCGCT	1 cut(s)	106
AfiI	CCNNNNNNNGG	6 cut(s)	125, 148, 780, 975, 1151, 1152
AflIII	ACRYGT	1 cut(s)	822
AgsI	TTSAA	5 cut(s)	9, 386, 565, 622, 1088
AjiI	CACGTC	1 cut(s)	823
AjnI	CCWGG	3 cut(s)	198, 501, 716
AjuI	GAANNNNNNNTTGG	2 cut(s)	195, 227
AluBI	AGCT	7 cut(s)	66, 208, 277, 389, 596, 758, 831
AluI	AGCT	7 cut(s)	66, 208, 277, 389, 596, 758, 831
Alw21I	GWGCWC	2 cut(s)	68, 660
AlwI	GGATC	3 cut(s)	249, 492, 505
AlwNI	CAGNNNCTG	1 cut(s)	30
Ama87I	CYCGRG	1 cut(s)	1162
Aor51HI	AGCGCT	1 cut(s)	106
AoxI	GGCC	3 cut(s)	771, 1055, 1219
ApeKI	GCWGC	2 cut(s)	841, 844
ApoI	RAATTY	1 cut(s)	903
AspA2I	CCTAGG	1 cut(s)	124
AspLEI	GCGC	2 cut(s)	107, 1211
AspS9I	GGNCC	3 cut(s)	772, 1166, 1219
AsuC2I	CCSGG	2 cut(s)	440, 1218
AsuHPI	GGTGA	2 cut(s)	113, 812
AvaI	CYCGRG	1 cut(s)	1162
AvaII	GGWCC	1 cut(s)	1166
AvrII	CCTAGG	1 cut(s)	124
BamHI	GGATCC	1 cut(s)	497
BanII	GRGCYC	2 cut(s)	24, 68
Bbv12I	GWGCWC	2 cut(s)	68, 660
BbvCI	CCTCAGC	1 cut(s)	1107
BbvI	GCAGC	2 cut(s)	828, 831
BccI	CCATC	7 cut(s)	29, 317, 407, 423, 738, 922, 1177
BciT130I	CCWGG	3 cut(s)	200, 503, 718
BciVI	GTATCC	1 cut(s)	422
BcnI	CCSGG	2 cut(s)	440, 1218
BfaI	CTAG	2 cut(s)	99, 125
BfmI	CTRYAG	2 cut(s)	1121, 1200
BfoI	RGCGCY	2 cut(s)	108, 1212
BfuI	GTATCC	1 cut(s)	422
BisI	GCNGC	2 cut(s)	842, 845
BlnI	CCTAGG	1 cut(s)	124
BlsI	GCNGC	2 cut(s)	843, 846
Bme1390I	CCNGG	5 cut(s)	200, 440, 503, 718, 1218
Bme18I	GGWCC	1 cut(s)	1166
BmeT110I	CYCGRG	1 cut(s)	1162
BmgBI	CACGTC	1 cut(s)	823
BmgT120I	GGNCC	3 cut(s)	772, 1166, 1219
BmiI	GGNNCC	4 cut(s)	437, 499, 774, 1167
BmrFI	CCNGG	5 cut(s)	200, 440, 503, 718, 1218
BmrI	ACTGGG	1 cut(s)	50
BmsI	GCATC	3 cut(s)	207, 1101, 1177
BmuI	ACTGGG	1 cut(s)	50
BoxI	GACNNNNGTC	1 cut(s)	882
BplI	GAGNNNNNCTC	6 cut(s)	103, 135, 1196, 1228, 1198, 1230
BpmI	CTGGAG	1 cut(s)	869
Bpu10I	CCTNAGC	1 cut(s)	1107
BpuEI	CTTGAG	1 cut(s)	935
BpuMI	CCSGG	2 cut(s)	440, 1218
BsaBI	GATNNNNATC	2 cut(s)	192, 221
BsaJI	CCNNGG	7 cut(s)	124, 439, 501, 502, 717, 1068, 1146
Bsc4I	CCNNNNNNNGG	6 cut(s)	125, 148, 780, 975, 1151, 1152
Bse1I	ACTGG	1 cut(s)	56
Bse8I	GATNNNNATC	2 cut(s)	192, 221
BseBI	CCWGG	3 cut(s)	200, 503, 718
BseDI	CCNNGG	7 cut(s)	124, 439, 501, 502, 717, 1068, 1146
BseGI	GGATG	1 cut(s)	222
BseJI	GATNNNNATC	2 cut(s)	192, 221
BseLI	CCNNNNNNNGG	6 cut(s)	125, 148, 780, 975, 1151, 1152
BseMII	CTCAG	5 cut(s)	37, 99, 312, 702, 1121
BseNI	ACTGG	1 cut(s)	56
BseRI	GAGGAG	2 cut(s)	725, 768
BseXI	GCAGC	2 cut(s)	828, 831
BsgI	GTGCAG	1 cut(s)	407
BshFI	GGCC	3 cut(s)	773, 1057, 1221
BsiHKAI	GWGCWC	2 cut(s)	68, 660
BsiHKCI	CYCGRG	1 cut(s)	1162
BsiSI	CCGG	2 cut(s)	439, 1217
BslFI	GGGAC	2 cut(s)	568, 1168
BslI	CCNNNNNNNGG	6 cut(s)	125, 148, 780, 975, 1151, 1152
BsmFI	GGGAC	2 cut(s)	568, 1168
BsmI	GAATGC	1 cut(s)	843
BsnI	GGCC	3 cut(s)	773, 1057, 1221
BsoBI	CYCGRG	1 cut(s)	1162
Bsp1286I	GDGCHC	3 cut(s)	24, 68, 660
Bsp1407I	TGTACA	1 cut(s)	825
Bsp143I	GATC	3 cut(s)	241, 497, 852
BspACI	CCGC	2 cut(s)	116, 1010
BspANI	GGCC	3 cut(s)	773, 1057, 1221
BspCNI	CTCAG	5 cut(s)	36, 100, 311, 701, 1120
BspLI	GGNNCC	4 cut(s)	437, 499, 774, 1167
BspMAI	CTGCAG	2 cut(s)	1125, 1204
BspPI	GGATC	3 cut(s)	249, 492, 505
BsrGI	TGTACA	1 cut(s)	825
BsrI	ACTGG	1 cut(s)	56
BssECI	CCNNGG	7 cut(s)	124, 439, 501, 502, 717, 1068, 1146
BssMI	GATC	3 cut(s)	241, 497, 852
BssNAI	GTATAC	1 cut(s)	1034
BssT1I	CCWWGG	3 cut(s)	124, 1068, 1146
Bst1107I	GTATAC	1 cut(s)	1034
Bst2UI	CCWGG	3 cut(s)	200, 503, 718
Bst4CI	ACNGT	3 cut(s)	233, 635, 1038
Bst6I	CTCTTC	1 cut(s)	528
BstAUI	TGTACA	1 cut(s)	825
BstDEI	CTNAG	5 cut(s)	23, 108, 298, 688, 1107
BstENI	CCTNNNNNAGG	1 cut(s)	123
BstF5I	GGATG	1 cut(s)	222
BstH2I	RGCGCY	2 cut(s)	108, 1212
BstHHI	GCGC	2 cut(s)	107, 1211
BstKTI	GATC	3 cut(s)	244, 500, 855
BstMBI	GATC	3 cut(s)	241, 497, 852
BstMWI	GCNNNNNNNGC	1 cut(s)	1208
BstNI	CCWGG	3 cut(s)	200, 503, 718
BstNSI	RCATGY	1 cut(s)	885
BstPAI	GACNNNNGTC	1 cut(s)	882
BstSCI	CCNGG	5 cut(s)	198, 438, 501, 716, 1216
BstSFI	CTRYAG	2 cut(s)	1121, 1200
BstV1I	GCAGC	2 cut(s)	828, 831
BstX2I	RGATCY	2 cut(s)	241, 497
BstXI	CCANNNNNNTGG	1 cut(s)	934
BstYI	RGATCY	2 cut(s)	241, 497
BstZ17I	GTATAC	1 cut(s)	1034
BsuI	GTATCC	1 cut(s)	422
BsuRI	GGCC	3 cut(s)	773, 1057, 1221
BtgZI	GCGATG	1 cut(s)	1200
BtrI	CACGTC	1 cut(s)	823
BtsCI	GGATG	1 cut(s)	222
BtsI	GCAGTG	2 cut(s)	579, 711
BtsIMutI	CAGTG	4 cut(s)	409, 579, 631, 711
CaiI	CAGNNNCTG	1 cut(s)	30
CfoI	GCGC	2 cut(s)	107, 1211
Cfr13I	GGNCC	3 cut(s)	772, 1166, 1219
CseI	GACGC	1 cut(s)	887
Csp6I	GTAC	2 cut(s)	484, 826
CviAII	CATG	6 cut(s)	92, 487, 605, 769, 882, 1045
CviQI	GTAC	2 cut(s)	484, 826
DdeI	CTNAG	5 cut(s)	23, 108, 298, 688, 1107
DpnI	GATC	3 cut(s)	243, 499, 854
DpnII	GATC	3 cut(s)	241, 497, 852
Eam1104I	CTCTTC	1 cut(s)	528
EarI	CTCTTC	1 cut(s)	528
EciI	GGCGGA	1 cut(s)	105
Ecl136II	GAGCTC	1 cut(s)	66
Eco130I	CCWWGG	3 cut(s)	124, 1068, 1146
Eco24I	GRGCYC	2 cut(s)	24, 68
Eco47I	GGWCC	1 cut(s)	1166
Eco47III	AGCGCT	1 cut(s)	106
Eco53kI	GAGCTC	1 cut(s)	66
Eco57I	CTGAAG	3 cut(s)	165, 192, 411
Eco88I	CYCGRG	1 cut(s)	1162
EcoICRI	GAGCTC	1 cut(s)	66
EcoNI	CCTNNNNNAGG	1 cut(s)	123
EcoRII	CCWGG	3 cut(s)	198, 501, 716
EcoT14I	CCWWGG	3 cut(s)	124, 1068, 1146
EcoT38I	GRGCYC	2 cut(s)	24, 68
ErhI	CCWWGG	3 cut(s)	124, 1068, 1146
FaeI	CATG	6 cut(s)	95, 490, 608, 772, 885, 1048
FalI	AAGNNNNNCTT	2 cut(s)	944, 976
FaqI	GGGAC	2 cut(s)	568, 1168
FatI	CATG	6 cut(s)	91, 486, 604, 768, 881, 1044
FblI	GTMKAC	1 cut(s)	1033
Fnu4HI	GCNGC	2 cut(s)	842, 845
FokI	GGATG	1 cut(s)	229
FriOI	GRGCYC	2 cut(s)	24, 68
Fsp4HI	GCNGC	2 cut(s)	842, 845
FspBI	CTAG	2 cut(s)	99, 125
GlaI	GCGC	2 cut(s)	106, 1210
GluI	GCNGC	2 cut(s)	842, 845
GsuI	CTGGAG	1 cut(s)	869
HaeII	RGCGCY	2 cut(s)	108, 1212
HaeIII	GGCC	3 cut(s)	773, 1057, 1221
HapII	CCGG	2 cut(s)	439, 1217
HgaI	GACGC	1 cut(s)	887
HhaI	GCGC	2 cut(s)	107, 1211
Hin1II	CATG	6 cut(s)	95, 490, 608, 772, 885, 1048
Hin6I	GCGC	2 cut(s)	105, 1209
HinP1I	GCGC	2 cut(s)	105, 1209
HincII	GTYRAC	1 cut(s)	991
HindII	GTYRAC	1 cut(s)	991
HindIII	AAGCTT	1 cut(s)	275
HinfI	GANTC	4 cut(s)	5, 112, 561, 965
HpaII	CCGG	2 cut(s)	439, 1217
HphI	GGTGA	2 cut(s)	113, 812
Hpy166II	GTNNAC	4 cut(s)	304, 826, 991, 1034
Hpy188I	TCNGA	5 cut(s)	184, 691, 811, 898, 1174
Hpy188III	TCNNGA	1 cut(s)	170
Hpy8I	GTNNAC	4 cut(s)	304, 826, 991, 1034
Hpy99I	CGWCG	1 cut(s)	753
HpyAV	CCTTC	3 cut(s)	142, 278, 935
HpyCH4III	ACNGT	3 cut(s)	233, 635, 1038
HpyCH4IV	ACGT	3 cut(s)	654, 822, 987
HpyCH4V	TGCA	8 cut(s)	74, 158, 254, 424, 452, 1123, 1179, 1202
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1208
HpyF3I	CTNAG	5 cut(s)	23, 108, 298, 688, 1107
HpySE526I	ACGT	3 cut(s)	654, 822, 987
Hsp92II	CATG	6 cut(s)	95, 490, 608, 772, 885, 1048
HspAI	GCGC	2 cut(s)	105, 1209
Kzo9I	GATC	3 cut(s)	241, 497, 852
LmnI	GCTCC	4 cut(s)	441, 663, 721, 755
Lsp1109I	GCAGC	2 cut(s)	828, 831
LweI	GCATC	3 cut(s)	207, 1101, 1177
MaeI	CTAG	2 cut(s)	99, 125
MaeII	ACGT	3 cut(s)	654, 822, 987
MaeIII	GTNAC	6 cut(s)	38, 227, 233, 394, 635, 884
MalI	GATC	3 cut(s)	243, 499, 854
MboI	GATC	3 cut(s)	241, 497, 852
MboII	GAAGA	9 cut(s)	185, 197, 355, 545, 620, 634, 876, 950, 952
MflI	RGATCY	2 cut(s)	241, 497
MhlI	GDGCHC	3 cut(s)	24, 68, 660
MluCI	AATT	7 cut(s)	210, 670, 816, 903, 1116, 1139, 1233
MlyI	GAGTC	2 cut(s)	121, 959
MmeI	TCCRAC	2 cut(s)	330, 915
MseI	TTAA	2 cut(s)	246, 1236
MslI	CAYNNNNRTG	2 cut(s)	572, 1027
MspI	CCGG	2 cut(s)	439, 1217
MspR9I	CCNGG	5 cut(s)	200, 440, 503, 718, 1218
Mva1269I	GAATGC	1 cut(s)	843
MvaI	CCWGG	3 cut(s)	200, 503, 718
MwoI	GCNNNNNNNGC	1 cut(s)	1208
NciI	CCSGG	2 cut(s)	440, 1218
NdeII	GATC	3 cut(s)	241, 497, 852
NlaIII	CATG	6 cut(s)	95, 490, 608, 772, 885, 1048
NlaIV	GGNNCC	4 cut(s)	437, 499, 774, 1167
NmuCI	GTSAC	2 cut(s)	635, 884
NspI	RCATGY	1 cut(s)	885
PasI	CCCWGGG	1 cut(s)	502
PctI	GAATGC	1 cut(s)	843
PfeI	GAWTC	2 cut(s)	5, 561
PfoI	TCCNGGA	1 cut(s)	198
PkrI	GCNGC	2 cut(s)	843, 846
PleI	GAGTC	2 cut(s)	120, 959
PpsI	GAGTC	2 cut(s)	120, 959
PshAI	GACNNNNGTC	1 cut(s)	882
Psp124BI	GAGCTC	1 cut(s)	68
Psp6I	CCWGG	3 cut(s)	198, 501, 716
PspGI	CCWGG	3 cut(s)	198, 501, 716
PspN4I	GGNNCC	4 cut(s)	437, 499, 774, 1167
PspPI	GGNCC	3 cut(s)	772, 1166, 1219
PstI	CTGCAG	2 cut(s)	1125, 1204
PstNI	CAGNNNCTG	1 cut(s)	30
PsuI	RGATCY	2 cut(s)	241, 497
RsaI	GTAC	2 cut(s)	485, 827
RsaNI	GTAC	2 cut(s)	484, 826
RseI	CAYNNNNRTG	2 cut(s)	572, 1027
SacI	GAGCTC	1 cut(s)	68
SaqAI	TTAA	2 cut(s)	246, 1236
SatI	GCNGC	2 cut(s)	842, 845
Sau3AI	GATC	3 cut(s)	241, 497, 852
Sau96I	GGNCC	3 cut(s)	772, 1166, 1219
SchI	GAGTC	2 cut(s)	121, 959
ScrFI	CCNGG	5 cut(s)	200, 440, 503, 718, 1218
SduI	GDGCHC	3 cut(s)	24, 68, 660
SfaNI	GCATC	3 cut(s)	207, 1101, 1177
SfcI	CTRYAG	2 cut(s)	1121, 1200
SinI	GGWCC	1 cut(s)	1166
SmiMI	CAYNNNNRTG	2 cut(s)	572, 1027
SmlI	CTYRAG	1 cut(s)	950
SmoI	CTYRAG	1 cut(s)	950
Sse9I	AATT	7 cut(s)	210, 670, 816, 903, 1116, 1139, 1233
SsiI	CCGC	2 cut(s)	116, 1010
SspMI	CTAG	2 cut(s)	99, 125
SstI	GAGCTC	1 cut(s)	68
StyD4I	CCNGG	5 cut(s)	198, 438, 501, 716, 1216
StyI	CCWWGG	3 cut(s)	124, 1068, 1146
TaaI	ACNGT	3 cut(s)	233, 635, 1038
TaiI	ACGT	3 cut(s)	657, 825, 990
TaqI	TCGA	3 cut(s)	331, 466, 963
TasI	AATT	7 cut(s)	210, 670, 816, 903, 1116, 1139, 1233
TatI	WGTACW	2 cut(s)	483, 825
TfiI	GAWTC	2 cut(s)	5, 561
Tru1I	TTAA	2 cut(s)	246, 1236
Tru9I	TTAA	2 cut(s)	246, 1236
TscAI	CASTG	4 cut(s)	409, 579, 638, 711
TseFI	GTSAC	2 cut(s)	635, 884
TseI	GCWGC	2 cut(s)	841, 844
Tsp45I	GTSAC	2 cut(s)	635, 884
TspDTI	ATGAA	4 cut(s)	546, 621, 915, 1152
TspGWI	ACGGA	2 cut(s)	215, 849
TspRI	CASTG	4 cut(s)	409, 579, 638, 711
VpaK11BI	GGWCC	1 cut(s)	1166
XagI	CCTNNNNNAGG	1 cut(s)	123
XapI	RAATTY	1 cut(s)	903
XceI	RCATGY	1 cut(s)	885
XmaJI	CCTAGG	1 cut(s)	124
XmiI	GTMKAC	1 cut(s)	1033
XspI	CTAG	2 cut(s)	99, 125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.