RLG00000027547

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004557
Physical Location & Seq
Reverse (-)
12590693 .. 12591129
437 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027547

Sequence Viewer

Length: 345 bp
ATGGATCGCCTGGAGCGCATGACATCCTTCTCGACGATTTCCAAGCGAAGGACGACAGCGGGGTCGACCCGGAGGGCGACCTGCCAAGTCAGCAGGGAGGGGAAGAGCGAGGAAGCCGAGGAAGGCTTCCGGCGACTCCGAAAGAGGAAGGAAGGTGAACAGGTCGGGCAGATCGGCGGTGACCTTCCTCCTGGCTCTCTTGGTAGCCTCGCTCACAACCATGGTAGATCTGAGCTTTGTCGAAAAAAACCATGTCCGGTGAAGCGACTCTGTGGTTTTGGGTCAGAGCAATCGCCGGTGACTTCATCCCGGTCAATCCGATCTGGTGCCCATAGCAATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

115

Amino Acids

12.56

Weight (kDa)

10.57

Isoelectric Point (pI)

73.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
Acc36I ACCTGC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 65
AciI CCGC 2 cut(s) 59, 177
AclWI GGATC 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 48
AjnI CCWGG 2 cut(s) 9, 190
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
AlwI GGATC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 18
AsuC2I CCSGG 2 cut(s) 70, 310
AsuHPI GGTGA 4 cut(s) 167, 191, 271, 310
BaeGI GKGCMC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 326
BciT130I CCWGG 2 cut(s) 11, 192
BcnI CCSGG 2 cut(s) 70, 310
BfaI CTAG 1 cut(s) 343
BfuAI ACCTGC 1 cut(s) 89
BglII AGATCT 1 cut(s) 227
Bme1390I CCNGG 4 cut(s) 11, 70, 192, 310
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 4 cut(s) 11, 70, 192, 310
BpmI CTGGAG 1 cut(s) 32
BpuMI CCSGG 2 cut(s) 70, 310
BsaJI CCNNGG 2 cut(s) 117, 220
BsaWI WCCGGW 1 cut(s) 256
Bsc4I CCNNNNNNNGG 1 cut(s) 48
Bse118I RCCGGY 1 cut(s) 295
BseBI CCWGG 2 cut(s) 11, 192
BseDI CCNNGG 2 cut(s) 117, 220
BseGI GGATG 2 cut(s) 23, 305
BseLI CCNNNNNNNGG 1 cut(s) 48
BseMII CTCAG 1 cut(s) 222
BseSI GKGCMC 1 cut(s) 331
BshNI GGYRCC 1 cut(s) 326
BsiSI CCGG 5 cut(s) 70, 130, 257, 296, 310
BslI CCNNNNNNNGG 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 331
Bsp143I GATC 4 cut(s) 4, 171, 227, 320
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 2 cut(s) 59, 177
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 328
BspMI ACCTGC 1 cut(s) 89
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 2 cut(s) 117, 220
BssMI GATC 4 cut(s) 4, 171, 227, 320
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 2 cut(s) 11, 192
Bst6I CTCTTC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 179
BstF5I GGATG 2 cut(s) 23, 305
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 4 cut(s) 7, 174, 230, 323
BstMBI GATC 4 cut(s) 4, 171, 227, 320
BstMWI GCNNNNNNNGC 2 cut(s) 15, 90
BstNI CCWGG 2 cut(s) 11, 192
BstPI GGTNACC 1 cut(s) 179
BstSCI CCNGG 4 cut(s) 9, 68, 190, 308
BstSLI GKGCMC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 23, 305
BveI ACCTGC 1 cut(s) 89
CfoI GCGC 1 cut(s) 18
Cfr10I RCCGGY 1 cut(s) 295
CviAII CATG 3 cut(s) 19, 221, 252
CviJI RGCY 5 cut(s) 116, 126, 195, 207, 235
CviKI_1 RGCY 5 cut(s) 116, 126, 195, 207, 235
DdeI CTNAG 1 cut(s) 231
DpnI GATC 4 cut(s) 6, 173, 229, 322
DpnII GATC 4 cut(s) 4, 171, 227, 320
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 220
Eco91I GGTNACC 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 9, 190
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 3 cut(s) 22, 224, 255
FaiI YATR 4 cut(s) 20, 222, 253, 333
FatI CATG 3 cut(s) 18, 220, 251
FauI CCCGC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 65
FokI GGATG 2 cut(s) 10, 292
FspBI CTAG 1 cut(s) 343
GlaI GCGC 1 cut(s) 17
GsuI CTGGAG 1 cut(s) 32
HapII CCGG 5 cut(s) 70, 130, 257, 296, 310
HhaI GCGC 1 cut(s) 18
Hin1II CATG 3 cut(s) 22, 224, 255
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 2 cut(s) 135, 267
HpaII CCGG 5 cut(s) 70, 130, 257, 296, 310
HphI GGTGA 4 cut(s) 167, 191, 271, 310
Hpy166II GTNNAC 2 cut(s) 66, 158
Hpy188I TCNGA 4 cut(s) 140, 232, 286, 320
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 2 cut(s) 66, 158
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 6 cut(s) 37, 42, 116, 142, 146, 194
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 90
HpyF3I CTNAG 1 cut(s) 231
Hsp92II CATG 3 cut(s) 22, 224, 255
HspAI GCGC 1 cut(s) 16
Kzo9I GATC 4 cut(s) 4, 171, 227, 320
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 13
MaeI CTAG 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 179, 298
MalI GATC 4 cut(s) 6, 173, 229, 322
MboI GATC 4 cut(s) 4, 171, 227, 320
MboII GAAGA 1 cut(s) 115
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 1 cut(s) 337
MlyI GAGTC 2 cut(s) 129, 261
MnlI CCTC 7 cut(s) 66, 91, 103, 112, 138, 198, 218
MslI CAYNNNNRTG 1 cut(s) 219
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 5 cut(s) 70, 130, 257, 296, 310
MspR9I CCNGG 4 cut(s) 11, 70, 192, 310
MvaI CCWGG 2 cut(s) 11, 192
MwoI GCNNNNNNNGC 2 cut(s) 15, 90
NciI CCSGG 2 cut(s) 70, 310
NcoI CCATGG 1 cut(s) 220
NdeII GATC 4 cut(s) 4, 171, 227, 320
NlaIII CATG 3 cut(s) 22, 224, 255
NlaIV GGNNCC 1 cut(s) 328
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 179, 298
PciSI GCTCTTC 1 cut(s) 98
PleI GAGTC 2 cut(s) 129, 261
PpsI GAGTC 2 cut(s) 129, 261
Psp6I CCWGG 2 cut(s) 9, 190
PspEI GGTNACC 1 cut(s) 179
PspGI CCWGG 2 cut(s) 9, 190
PspN4I GGNNCC 1 cut(s) 328
PsuI RGATCY 1 cut(s) 227
RseI CAYNNNNRTG 1 cut(s) 219
SalI GTCGAC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 98
Sau3AI GATC 4 cut(s) 4, 171, 227, 320
SchI GAGTC 2 cut(s) 129, 261
ScrFI CCNGG 4 cut(s) 11, 70, 192, 310
SduI GDGCHC 1 cut(s) 331
SetI ASST 5 cut(s) 83, 157, 165, 186, 237
SgrAI CRCCGGYG 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 219
Sse9I AATT 1 cut(s) 337
SsiI CCGC 2 cut(s) 59, 177
SspMI CTAG 1 cut(s) 343
StyD4I CCNGG 4 cut(s) 9, 68, 190, 308
StyI CCWWGG 1 cut(s) 220
TaqI TCGA 3 cut(s) 32, 65, 241
TasI AATT 1 cut(s) 337
TseFI GTSAC 2 cut(s) 179, 298
Tsp45I GTSAC 2 cut(s) 179, 298
TspDTI ATGAA 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 65
XspI CTAG 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.