Rh5DG116300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004557
Physical Location & Seq
Reverse (-)
10542993 .. 10543294
302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG116300.1

Sequence Viewer

Length: 258 bp
ATGGATCGCCTGGAGCGCATGACGTCCTCCTCGACGATATCCAAGGGAAGGACGACAGTGGGGTCGACCCGGAGGGCGATCTGCCAGGTCAGCAGGGAGGGGAAGAGCGAGGAAGCCGAGGAAGGCTTCCGGCGACTCCGGAGGAGGAGGGAAGGTGAACAGGTCGAGCAGATCGGCAGTGACCTTCCTCCTGGCTCTCTTGGTAGCCTCGTTCACAACCATGGTAGATATGAGCTTTGTCGAAAAAAACCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

9.71

Weight (kDa)

10.15

Isoelectric Point (pI)

62.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AatII GACGTC 1 cut(s) 26
AccI GTMKAC 1 cut(s) 65
AccIII TCCGGA 1 cut(s) 138
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 23
AfiI CCNNNNNNNGG 1 cut(s) 48
AjnI CCWGG 3 cut(s) 9, 84, 190
AluBI AGCT 1 cut(s) 235
AluI AGCT 1 cut(s) 235
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 138
AspLEI GCGC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 167
BciT130I CCWGG 3 cut(s) 11, 86, 192
BcnI CCSGG 1 cut(s) 70
Bme1390I CCNGG 4 cut(s) 11, 70, 86, 192
BmrFI CCNGG 4 cut(s) 11, 70, 86, 192
BpmI CTGGAG 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 4 cut(s) 42, 117, 220, 251
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 1 cut(s) 48
BseAI TCCGGA 1 cut(s) 138
BseBI CCWGG 3 cut(s) 11, 86, 192
BseDI CCNNGG 4 cut(s) 42, 117, 220, 251
BseLI CCNNNNNNNGG 1 cut(s) 48
BseRI GAGGAG 3 cut(s) 19, 157, 160
BsiSI CCGG 3 cut(s) 70, 130, 139
BslI CCNNNNNNNGG 1 cut(s) 48
Bsp13I TCCGGA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 4, 78, 171
Bsp19I CCATGG 2 cut(s) 220, 251
BspEI TCCGGA 1 cut(s) 138
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 42, 117, 220, 251
BssMI GATC 3 cut(s) 4, 78, 171
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 3 cut(s) 42, 220, 251
Bst2UI CCWGG 3 cut(s) 11, 86, 192
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 23
BstDSI CCRYGG 2 cut(s) 220, 251
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 3 cut(s) 7, 81, 174
BstMBI GATC 3 cut(s) 4, 78, 171
BstMWI GCNNNNNNNGC 2 cut(s) 15, 90
BstNI CCWGG 3 cut(s) 11, 86, 192
BstSCI CCNGG 4 cut(s) 9, 68, 84, 190
BtgI CCRYGG 2 cut(s) 220, 251
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 63, 184
CfoI GCGC 1 cut(s) 18
CviAII CATG 3 cut(s) 19, 221, 252
CviJI RGCY 5 cut(s) 116, 126, 195, 207, 235
CviKI_1 RGCY 5 cut(s) 116, 126, 195, 207, 235
DpnI GATC 3 cut(s) 6, 80, 173
DpnII GATC 3 cut(s) 4, 78, 171
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 3 cut(s) 42, 220, 251
Eco32I GATATC 1 cut(s) 39
EcoRII CCWGG 3 cut(s) 9, 84, 190
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 3 cut(s) 42, 220, 251
ErhI CCWWGG 3 cut(s) 42, 220, 251
FaeI CATG 3 cut(s) 22, 224, 255
FaiI YATR 4 cut(s) 20, 222, 231, 253
FatI CATG 3 cut(s) 18, 220, 251
FblI GTMKAC 1 cut(s) 65
GlaI GCGC 1 cut(s) 17
GsuI CTGGAG 1 cut(s) 32
HapII CCGG 3 cut(s) 70, 130, 139
HhaI GCGC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 22, 224, 255
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 1 cut(s) 135
HpaII CCGG 3 cut(s) 70, 130, 139
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 3 cut(s) 66, 158, 214
Hpy188III TCNNGA 1 cut(s) 139
Hpy8I GTNNAC 3 cut(s) 66, 158, 214
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 4 cut(s) 42, 116, 146, 194
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 23
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 90
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 22, 224, 255
HspAI GCGC 1 cut(s) 16
Kpn2I TCCGGA 1 cut(s) 138
Kzo9I GATC 3 cut(s) 4, 78, 171
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 13
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 179
MalI GATC 3 cut(s) 6, 80, 173
MboI GATC 3 cut(s) 4, 78, 171
MboII GAAGA 1 cut(s) 115
MlyI GAGTC 1 cut(s) 129
MroI TCCGGA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 3 cut(s) 70, 130, 139
MspR9I CCNGG 4 cut(s) 11, 70, 86, 192
MvaI CCWGG 3 cut(s) 11, 86, 192
MwoI GCNNNNNNNGC 2 cut(s) 15, 90
NciI CCSGG 1 cut(s) 70
NcoI CCATGG 2 cut(s) 220, 251
NdeII GATC 3 cut(s) 4, 78, 171
NlaIII CATG 3 cut(s) 22, 224, 255
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp6I CCWGG 3 cut(s) 9, 84, 190
PspGI CCWGG 3 cut(s) 9, 84, 190
RseI CAYNNNNRTG 1 cut(s) 219
SalI GTCGAC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 98
Sau3AI GATC 3 cut(s) 4, 78, 171
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 4 cut(s) 11, 70, 86, 192
SetI ASST 6 cut(s) 26, 90, 157, 165, 186, 237
SmiMI CAYNNNNRTG 1 cut(s) 219
StyD4I CCNGG 4 cut(s) 9, 68, 84, 190
StyI CCWWGG 3 cut(s) 42, 220, 251
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 26
TaqI TCGA 4 cut(s) 32, 65, 165, 241
TscAI CASTG 2 cut(s) 63, 184
TseFI GTSAC 1 cut(s) 179
Tsp45I GTSAC 1 cut(s) 179
TspRI CASTG 2 cut(s) 63, 184
XmiI GTMKAC 1 cut(s) 65
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.