Rh2BG435500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004557
Physical Location & Seq
Forward (+)
61738824 .. 61739243
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG435500.1

Sequence Viewer

Length: 420 bp
ATGGATCGCCTGGAGCGCATGACGTCCTCCTCGACGATATCCAAGGGAAGTACGACAGTGGGGTCAACCCGGAGGGCGATCTGCCAGGTCAGTAGGGAGGGGAAGAGCGAGGAAGCCCAGGAAGGCTTCTGGCTACTCCGGAGGAGGAGGGAAGGTGAACAGGTTGGGCAGATCGGCAGCGACCTTCCTCTTCACGATCTGCAGAAGCTAGAGGAAGCTGAGGAAGGCTTCCGGCGACTCCGGAGGAGGAGGGAAGGTGAACAGGTCGGGCAGATTGGCGGCGACCTTCATCTTGGCTCTTTTGGTAGCCTCGTTCACAACCATGGGAGATCTGAGCTTTGTCGAAAAAACCATGGTAGATCTGAGAGCCTAGCTTCTGCAGATCGTGAAGTGAAGGTTGATGCTTCTAGGGTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.61

Weight (kDa)

9.23

Isoelectric Point (pI)

69.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 61
AatII GACGTC 1 cut(s) 26
AccIII TCCGGA 2 cut(s) 138, 240
AciI CCGC 1 cut(s) 279
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 52
AjnI CCWGG 3 cut(s) 9, 84, 117
AluBI AGCT 4 cut(s) 208, 218, 337, 374
AluI AGCT 4 cut(s) 208, 218, 337, 374
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 2 cut(s) 138, 240
ApeKI GCWGC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 167, 269
BbvCI CCTCAGC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 189
BciT130I CCWGG 3 cut(s) 11, 86, 119
BcnI CCSGG 1 cut(s) 70
BfaI CTAG 3 cut(s) 209, 371, 408
BfmI CTRYAG 2 cut(s) 200, 378
BglII AGATCT 2 cut(s) 329, 359
BisI GCNGC 2 cut(s) 178, 280
BlsI GCNGC 2 cut(s) 179, 281
Bme1390I CCNGG 4 cut(s) 11, 70, 86, 119
BmrFI CCNGG 4 cut(s) 11, 70, 86, 119
BmsI GCATC 1 cut(s) 391
BpmI CTGGAG 1 cut(s) 32
Bpu10I CCTNAGC 1 cut(s) 219
BpuMI CCSGG 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 4 cut(s) 42, 117, 322, 352
BsaWI WCCGGW 2 cut(s) 138, 240
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 241, 271
BseAI TCCGGA 2 cut(s) 138, 240
BseBI CCWGG 3 cut(s) 11, 86, 119
BseDI CCNNGG 4 cut(s) 42, 117, 322, 352
BseMII CTCAG 3 cut(s) 210, 324, 354
BseRI GAGGAG 5 cut(s) 19, 157, 160, 259, 262
BseXI GCAGC 1 cut(s) 189
BsiSI CCGG 4 cut(s) 70, 139, 232, 241
Bsp13I TCCGGA 2 cut(s) 138, 240
Bsp143I GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
Bsp19I CCATGG 2 cut(s) 322, 352
BspACI CCGC 1 cut(s) 279
BspCNI CTCAG 3 cut(s) 211, 325, 355
BspEI TCCGGA 2 cut(s) 138, 240
BspMAI CTGCAG 2 cut(s) 204, 382
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 98
BssECI CCNNGG 4 cut(s) 42, 117, 322, 352
BssMI GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 3 cut(s) 42, 322, 352
Bst2UI CCWGG 3 cut(s) 11, 86, 119
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 98, 195
BstACI GRCGYC 1 cut(s) 23
BstDEI CTNAG 3 cut(s) 219, 333, 363
BstDSI CCRYGG 2 cut(s) 322, 352
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 7 cut(s) 7, 81, 174, 199, 332, 362, 385
BstMBI GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstNI CCWGG 3 cut(s) 11, 86, 119
BstSCI CCNGG 4 cut(s) 9, 68, 84, 117
BstSFI CTRYAG 2 cut(s) 200, 378
BstV1I GCAGC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 329, 359
BstYI RGATCY 2 cut(s) 329, 359
BtgI CCRYGG 2 cut(s) 322, 352
BtsIMutI CAGTG 1 cut(s) 63
CfoI GCGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 51
CviAII CATG 3 cut(s) 19, 323, 353
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 3 cut(s) 219, 333, 363
DpnI GATC 7 cut(s) 6, 80, 173, 198, 331, 361, 384
DpnII GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
DrdI GACNNNNNNGTC 1 cut(s) 61
DseDI GACNNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 98, 195
EarI CTCTTC 2 cut(s) 98, 195
Eco130I CCWWGG 3 cut(s) 42, 322, 352
Eco32I GATATC 1 cut(s) 39
EcoRII CCWGG 3 cut(s) 9, 84, 117
EcoRV GATATC 1 cut(s) 39
EcoT14I CCWWGG 3 cut(s) 42, 322, 352
ErhI CCWWGG 3 cut(s) 42, 322, 352
FaeI CATG 3 cut(s) 22, 326, 356
FaiI YATR 3 cut(s) 20, 324, 354
FatI CATG 3 cut(s) 18, 322, 352
Fnu4HI GCNGC 2 cut(s) 178, 280
Fsp4HI GCNGC 2 cut(s) 178, 280
FspBI CTAG 3 cut(s) 209, 371, 408
GlaI GCGC 1 cut(s) 17
GluI GCNGC 2 cut(s) 178, 280
GsuI CTGGAG 1 cut(s) 32
HapII CCGG 4 cut(s) 70, 139, 232, 241
HhaI GCGC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 22, 326, 356
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 1 cut(s) 237
HpaII CCGG 4 cut(s) 70, 139, 232, 241
HphI GGTGA 2 cut(s) 167, 269
Hpy166II GTNNAC 4 cut(s) 66, 158, 260, 316
Hpy188I TCNGA 2 cut(s) 334, 364
Hpy188III TCNNGA 4 cut(s) 139, 194, 241, 386
Hpy8I GTNNAC 4 cut(s) 66, 158, 260, 316
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 7 cut(s) 116, 146, 194, 218, 248, 296, 388
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 202, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
HpyF3I CTNAG 3 cut(s) 219, 333, 363
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 22, 326, 356
HspAI GCGC 1 cut(s) 16
Kpn2I TCCGGA 2 cut(s) 138, 240
Kzo9I GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 1 cut(s) 391
MaeI CTAG 3 cut(s) 209, 371, 408
MaeII ACGT 1 cut(s) 23
MalI GATC 7 cut(s) 6, 80, 173, 198, 331, 361, 384
MboI GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
MboII GAAGA 2 cut(s) 115, 182
MflI RGATCY 2 cut(s) 329, 359
MluCI AATT 1 cut(s) 415
MlyI GAGTC 1 cut(s) 231
MroI TCCGGA 2 cut(s) 138, 240
MseI TTAA 2 cut(s) 414, 418
MslI CAYNNNNRTG 1 cut(s) 321
MspI CCGG 4 cut(s) 70, 139, 232, 241
MspR9I CCNGG 4 cut(s) 11, 70, 86, 119
MvaI CCWGG 3 cut(s) 11, 86, 119
MwoI GCNNNNNNNGC 1 cut(s) 15
NciI CCSGG 1 cut(s) 70
NcoI CCATGG 2 cut(s) 322, 352
NdeII GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
NlaIII CATG 3 cut(s) 22, 326, 356
PacI TTAATTAA 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PkrI GCNGC 2 cut(s) 179, 281
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
Psp6I CCWGG 3 cut(s) 9, 84, 117
PspGI CCWGG 3 cut(s) 9, 84, 117
PstI CTGCAG 2 cut(s) 204, 382
PsuI RGATCY 2 cut(s) 329, 359
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 414, 418
SatI GCNGC 2 cut(s) 178, 280
Sau3AI GATC 7 cut(s) 4, 78, 171, 196, 329, 359, 382
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 4 cut(s) 11, 70, 86, 119
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 2 cut(s) 200, 378
SmiMI CAYNNNNRTG 1 cut(s) 321
Sse9I AATT 1 cut(s) 415
SsiI CCGC 1 cut(s) 279
SspMI CTAG 3 cut(s) 209, 371, 408
StyD4I CCNGG 4 cut(s) 9, 68, 84, 117
StyI CCWWGG 3 cut(s) 42, 322, 352
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 32, 343
TasI AATT 1 cut(s) 415
TauI GCSGC 1 cut(s) 282
Tru1I TTAA 2 cut(s) 414, 418
Tru9I TTAA 2 cut(s) 414, 418
TscAI CASTG 1 cut(s) 63
TseI GCWGC 1 cut(s) 177
TspDTI ATGAA 1 cut(s) 278
TspRI CASTG 1 cut(s) 63
XspI CTAG 3 cut(s) 209, 371, 408
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.