Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0001580

Physical Location & Seq

Genomic Coordinates Forward (+)

53160731 .. 53162469

Gene Structure 1739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030002

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGATTTCTGGGTATGCAATGCACCAGTTGGGTGGAAATGTGTTAGAGGTTTTTATACTGATGCGTAGGAGTGAGGAGAGGGAAGAAGAGAATGAGTTTGTTCTGACCGCGGTTCTTAGTGGTTTGGCGGTTCCTGAATTTGTTGATACAGGCAAGCAGATTCATTGCCTTGCGGTGAAAAATGGGCTGATGTGTAATGTTTCTGTTGGGAATGCGCTTGTCACGATGTATGCGAAATGTGGGAGTTTAGATTATGCGCTTAGAATTTTTGAGCTGTCTGGTGATAAGAATTCTATCACATGGTCTGCGATGATATCTGGTTTTGCGCAGAGTAGGGATTCGCAAAAGGCTTTGGACTTGTTTTCAGACATGCATTTTTCTGGGATAATGCCGACGGAGTTTGCGTTTGGTGGGGTCATCAGTGCCTGTAGTGACATCAATGCTCTTGAACAAGGAAAGCAAGTGCATAGTTATCTGCTCAAGTTGGGATTTGAATTCCAATTATATATAATTTCAGCTTTGGTTGACATGTATGGCAAATGTGGTAGCGTTAGTGATGCTCGGAAAGGGTTTGATTGTATAAAAGAGCCTGATATTGTTCTTTGGAGTTCTATGATCGGAGGGTATGTACAAAATGGGGAGAATGAAGCTGCTTTGAGTTTATACTGTAGAATGCAGAGGGAGGGATTTTTGCCCAATGAACTCACTATGGCCAGTCTCCTGAAAGCATGTTCAAGCCTATGTGCTTTTGAACAAGGAAAGCAAATCCATGTGAGTACCATTAAGTATGGATTCAGTCTTGAATTTCCAATTGGAAGTTCTCTTTTCACTATGTATGCAAAGTGTGGGAATTTGGAAGATGGGAAGTTAGTGTTTAGGAGAATGCCTACGAGAGATGTAGTGTCTTGGAATGCAATGATATCTGGGCTTTCACAAAATGGGCAGGGTAACGAAGCTCTAGAGCTCTTTGAGGAGATGCGATTGGGTGGCACAGAGCCAGATTACGTTACATTTGTGAATGTTCTCTCTGCTTGCAGCCATATGGGGTCTGTGGAGAGAGGATGGATTTATTTCAAGATGATGTCTAATGAATTTGGCATTTCCCCAAGGGTGGAGCATTATGCTTGCATGGTTGATGTCCTGAGCCGTGCTGGGAAGTTAGATGAAGCCAAAGATTTCATAGAGTCAGCAACTATTGATCATGGCATCTGTTTATGGCGTATTTTATTAAGTGCTTGTCGAAATTATCGGAATTATGAATTGGGAGCATATGCCGGAGAGAAGCTAATGGAGTTAGGTTCACTAGAATCACAGGCTTACGTGCTATTGTCAAGCATCTATACTTCCCTGGGTTGGCGGGAAGATGTTGAGCGGGTAAGGAGGATGATGAAACTTCGAGGGGTGAGTAAGAAGCCTGGCTGTAGCTGGATTGAGTTGAAGAGTCAGGTTCATGTGTTTGTTGTTGGTGATGTGATGCATCCACAGATTGATAGTATTGGTGTTGAGATCCGAAGGTTAATCAAGCATATGAAGGATGAAGGATACCAACCTTCCTCTACCACTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

523

Amino Acids

58.22

Weight (kDa)

5.59

Isoelectric Point (pI)

44.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	96 - 144	7.5e-09	PPR repeat family
PPR	PF01535	99 - 129	2.2e-06	PPR repeat
PPR_2	PF13041	197 - 245	7.4e-12	PPR repeat family
PPR	PF01535	200 - 230	2.9e-07	PPR repeat
PPR_2	PF13041	298 - 346	2.1e-13	PPR repeat family
PPR_1	PF12854	299 - 327	1e-06	PPR repeat
PPR	PF01535	301 - 327	3e-09	PPR repeat
E_motif	PF20431	416 - 478	2.2e-18	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001580)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33680 AT2G33680 AT2G33680 AT2G33680 AT2G33680 AT2G33680 AT2G33680 AT2G33680
fragaria_vesca	FvH4_7g03841 FvH4_7g03850
malus_domestica	MD02G1276700.v1.1
prunus_persica	Prupe.2G043400_v2.0.a1
pyrus_communis	pycom02g23700
rosa_chinensis	RchiOBHm_Chr1g0326811 RchiOBHm_Chr1g0326821 RchiOBHm_Chr1g0327271 RchiOBHm_Chr1g0327321
rosa_laevigata	RLG00000030002 RLG00000030005 RLG00000030054
rosa_multiflora	Rmu_sc0001021.1_g000002 Rmu_sc0003600.1_g000043 Rmu_sc0005038.1_g000015 Rmu_sc0010151.1_g000003 Rmu_sc0010151.1_g000004 Rmu_sc0020365.1_g000001
rosa_roxburghii	Rroxscaffold_2G00128000 Rroxscaffold_4G00322770 Rroxscaffold_4G00322870
rosa_rugosa	Rorug01G0064900 Rorug01G0065300 Rorug01G0065400 Rorug01G0065500 Rorug01G0065500
rosa_samantha	Rh1AG082500 Rh1AG083100 Rh1BG065600 Rh1BG065900 Rh1BG066000 Rh1CG080500 Rh1DG087500 Rh2AG148500 Rh2AG148600 Rh2BG154000 Rh2DG154200 Rh3CG329400
rosa_wichuraiana	Rw1G006500 Rw1G006530 Rw2G011590 Rw2G011600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	327
AccBSI	CCGCTC	1 cut(s)	1372
AccII	CGCG	1 cut(s)	110
AciI	CCGC	6 cut(s)	108, 110, 128, 173, 1357, 1372
AclWI	GGATC	1 cut(s)	1501
AcoI	YGGCCR	1 cut(s)	711
AcsI	RAATTY	7 cut(s)	137, 264, 289, 494, 803, 850, 1091
AfaI	GTAC	2 cut(s)	630, 778
AfiI	CCNNNNNNNGG	2 cut(s)	1111, 1353
AflIII	ACRYGT	1 cut(s)	528
AgsI	TTSAA	7 cut(s)	449, 494, 735, 752, 803, 1075, 1438
AjnI	CCWGG	2 cut(s)	1347, 1414
AjuI	GAANNNNNNNTTGG	6 cut(s)	795, 802, 827, 834, 1244, 1276
AluBI	AGCT	7 cut(s)	274, 518, 650, 956, 964, 1285, 1425
AluI	AGCT	7 cut(s)	274, 518, 650, 956, 964, 1285, 1425
Alw21I	GWGCWC	1 cut(s)	966
Alw26I	GTCTC	1 cut(s)	722
AlwI	GGATC	1 cut(s)	1501
AlwNI	CAGNNNCTG	1 cut(s)	426
AoxI	GGCC	1 cut(s)	711
ApeKI	GCWGC	2 cut(s)	650, 1035
ApoI	RAATTY	7 cut(s)	137, 264, 289, 494, 803, 850, 1091
AspLEI	GCGC	3 cut(s)	217, 259, 328
AsuHPI	GGTGA	4 cut(s)	187, 293, 1414, 1478
BalI	TGGCCA	1 cut(s)	713
BanII	GRGCYC	1 cut(s)	966
Bbv12I	GWGCWC	1 cut(s)	966
BbvI	GCAGC	2 cut(s)	637, 1047
BccI	CCATC	2 cut(s)	854, 1056
BceAI	ACGGC	1 cut(s)	1131
BciT130I	CCWGG	2 cut(s)	1349, 1416
BciVI	GTATCC	1 cut(s)	1535
BclI	TGATCA	1 cut(s)	1198
BcoDI	GTCTC	1 cut(s)	722
BfaI	CTAG	2 cut(s)	959, 1304
BfmI	CTRYAG	3 cut(s)	427, 667, 1420
BfuI	GTATCC	1 cut(s)	1535
BisI	GCNGC	2 cut(s)	651, 1036
BlsI	GCNGC	2 cut(s)	652, 1037
Bme1390I	CCNGG	2 cut(s)	1349, 1416
BmiI	GGNNCC	1 cut(s)	132
BmrFI	CCNGG	2 cut(s)	1349, 1416
BmsI	GCATC	7 cut(s)	51, 547, 966, 1215, 1344, 1464, 1486
Bpu10I	CCTNAGC	1 cut(s)	1142
BpuEI	CTTGAG	1 cut(s)	464
BsaAI	YACGTR	1 cut(s)	1321
BsaJI	CCNNGG	4 cut(s)	108, 1106, 1347, 1348
BsaXI	ACNNNNNCTCC	4 cut(s)	389, 419, 612, 642
Bsc4I	CCNNNNNNNGG	2 cut(s)	1111, 1353
Bse1I	ACTGG	2 cut(s)	25, 714
Bse3DI	GCAATG	3 cut(s)	24, 163, 921
BseBI	CCWGG	2 cut(s)	1349, 1416
BseDI	CCNNGG	4 cut(s)	108, 1106, 1347, 1348
BseGI	GGATG	4 cut(s)	1067, 1389, 1477, 1540
BseLI	CCNNNNNNNGG	2 cut(s)	1111, 1353
BseMI	GCAATG	3 cut(s)	24, 163, 921
BseMII	CTCAG	1 cut(s)	1133
BseNI	ACTGG	2 cut(s)	25, 714
BseRI	GAGGAG	2 cut(s)	89, 986
BseXI	GCAGC	2 cut(s)	637, 1047
BseYI	CCCAGC	1 cut(s)	1151
Bsh1236I	CGCG	1 cut(s)	110
BshFI	GGCC	1 cut(s)	713
BsiHKAI	GWGCWC	1 cut(s)	966
BsiSI	CCGG	1 cut(s)	1275
BslI	CCNNNNNNNGG	2 cut(s)	1111, 1353
BsmAI	GTCTC	1 cut(s)	722
BsmI	GAATGC	4 cut(s)	217, 678, 888, 916
BsnI	GGCC	1 cut(s)	713
Bsp1286I	GDGCHC	1 cut(s)	966
Bsp1407I	TGTACA	1 cut(s)	628
Bsp143I	GATC	3 cut(s)	615, 1198, 1506
BspACI	CCGC	6 cut(s)	108, 110, 128, 173, 1357, 1372
BspANI	GGCC	1 cut(s)	713
BspCNI	CTCAG	1 cut(s)	1134
BspFNI	CGCG	1 cut(s)	110
BspLI	GGNNCC	1 cut(s)	132
BspPI	GGATC	1 cut(s)	1501
BsrBI	CCGCTC	1 cut(s)	1372
BsrDI	GCAATG	3 cut(s)	24, 163, 921
BsrGI	TGTACA	1 cut(s)	628
BsrI	ACTGG	2 cut(s)	25, 714
BssECI	CCNNGG	4 cut(s)	108, 1106, 1347, 1348
BssMI	GATC	3 cut(s)	615, 1198, 1506
BssT1I	CCWWGG	1 cut(s)	1106
Bst2UI	CCWGG	2 cut(s)	1349, 1416
Bst4CI	ACNGT	1 cut(s)	668
Bst6I	CTCTTC	2 cut(s)	81, 1433
BstAUI	TGTACA	1 cut(s)	628
BstBAI	YACGTR	1 cut(s)	1321
BstC8I	GCNNGC	3 cut(s)	155, 1033, 1126
BstDEI	CTNAG	3 cut(s)	116, 260, 1142
BstDSI	CCRYGG	1 cut(s)	108
BstF5I	GGATG	4 cut(s)	1067, 1389, 1477, 1540
BstFNI	CGCG	1 cut(s)	110
BstHHI	GCGC	3 cut(s)	217, 259, 328
BstKTI	GATC	3 cut(s)	618, 1201, 1509
BstMAI	GTCTC	1 cut(s)	722
BstMBI	GATC	3 cut(s)	615, 1198, 1506
BstNI	CCWGG	2 cut(s)	1349, 1416
BstNSI	RCATGY	3 cut(s)	373, 532, 732
BstSCI	CCNGG	2 cut(s)	1347, 1414
BstSFI	CTRYAG	3 cut(s)	427, 667, 1420
BstUI	CGCG	1 cut(s)	110
BstV1I	GCAGC	2 cut(s)	637, 1047
BstX2I	RGATCY	1 cut(s)	1506
BstXI	CCANNNNNNTGG	1 cut(s)	32
BstYI	RGATCY	1 cut(s)	1506
BsuI	GTATCC	1 cut(s)	1535
BsuRI	GGCC	1 cut(s)	713
BtgI	CCRYGG	1 cut(s)	108
BtgZI	GCGATG	1 cut(s)	323
BtsCI	GGATG	4 cut(s)	1067, 1389, 1477, 1540
BtsIMutI	CAGTG	1 cut(s)	427
Cac8I	GCNNGC	3 cut(s)	155, 1033, 1126
CaiI	CAGNNNCTG	1 cut(s)	426
CfoI	GCGC	3 cut(s)	217, 259, 328
Cfr42I	CCGCGG	1 cut(s)	111
Csp6I	GTAC	2 cut(s)	629, 777
CviAII	CATG	8 cut(s)	300, 370, 529, 729, 770, 1129, 1202, 1451
CviQI	GTAC	2 cut(s)	629, 777
DdeI	CTNAG	3 cut(s)	116, 260, 1142
DpnI	GATC	3 cut(s)	617, 1200, 1508
DpnII	GATC	3 cut(s)	615, 1198, 1506
EaeI	YGGCCR	1 cut(s)	711
Eam1104I	CTCTTC	2 cut(s)	81, 1433
EarI	CTCTTC	2 cut(s)	81, 1433
Ecl136II	GAGCTC	1 cut(s)	964
Eco130I	CCWWGG	1 cut(s)	1106
Eco24I	GRGCYC	1 cut(s)	966
Eco32I	GATATC	2 cut(s)	315, 921
Eco53kI	GAGCTC	1 cut(s)	964
EcoICRI	GAGCTC	1 cut(s)	964
EcoRI	GAATTC	2 cut(s)	289, 494
EcoRII	CCWGG	2 cut(s)	1347, 1414
EcoRV	GATATC	2 cut(s)	315, 921
EcoT14I	CCWWGG	1 cut(s)	1106
EcoT22I	ATGCAT	2 cut(s)	375, 1479
EcoT38I	GRGCYC	1 cut(s)	966
ErhI	CCWWGG	1 cut(s)	1106
FaeI	CATG	8 cut(s)	303, 373, 532, 732, 773, 1132, 1205, 1454
FatI	CATG	8 cut(s)	299, 369, 528, 728, 769, 1128, 1201, 1450
FauI	CCCGC	2 cut(s)	1350, 1365
FauNDI	CATATG	3 cut(s)	1041, 1270, 1527
FbaI	TGATCA	1 cut(s)	1198
Fnu4HI	GCNGC	2 cut(s)	651, 1036
FokI	GGATG	4 cut(s)	1074, 1396, 1464, 1547
FriOI	GRGCYC	1 cut(s)	966
Fsp4HI	GCNGC	2 cut(s)	651, 1036
FspBI	CTAG	2 cut(s)	959, 1304
FspI	TGCGCA	1 cut(s)	327
GlaI	GCGC	3 cut(s)	216, 258, 327
GluI	GCNGC	2 cut(s)	651, 1036
GsaI	CCCAGC	1 cut(s)	1155
HaeIII	GGCC	1 cut(s)	713
HapII	CCGG	1 cut(s)	1275
HhaI	GCGC	3 cut(s)	217, 259, 328
Hin1II	CATG	8 cut(s)	303, 373, 532, 732, 773, 1132, 1205, 1454
Hin6I	GCGC	3 cut(s)	215, 257, 326
HinP1I	GCGC	3 cut(s)	215, 257, 326
HincII	GTYRAC	1 cut(s)	526
HindII	GTYRAC	1 cut(s)	526
HinfI	GANTC	6 cut(s)	160, 338, 792, 1184, 1307, 1441
HpaII	CCGG	1 cut(s)	1275
HphI	GGTGA	4 cut(s)	187, 293, 1414, 1478
Hpy166II	GTNNAC	2 cut(s)	526, 1301
Hpy188I	TCNGA	6 cut(s)	105, 367, 564, 620, 1251, 1511
Hpy188III	TCNNGA	8 cut(s)	134, 223, 446, 721, 800, 959, 1075, 1141
Hpy8I	GTNNAC	2 cut(s)	526, 1301
Hpy99I	CGWCG	1 cut(s)	397
HpyAV	CCTTC	4 cut(s)	1506, 1525, 1532, 1560
HpyCH4III	ACNGT	1 cut(s)	668
HpyCH4IV	ACGT	2 cut(s)	1005, 1320
HpyF3I	CTNAG	3 cut(s)	116, 260, 1142
HpySE526I	ACGT	2 cut(s)	1005, 1320
Hsp92II	CATG	8 cut(s)	303, 373, 532, 732, 773, 1132, 1205, 1454
HspAI	GCGC	3 cut(s)	215, 257, 326
Ksp22I	TGATCA	1 cut(s)	1198
KspI	CCGCGG	1 cut(s)	111
Kzo9I	GATC	3 cut(s)	615, 1198, 1506
LmnI	GCTCC	2 cut(s)	1114, 1265
Lsp1109I	GCAGC	2 cut(s)	637, 1047
LweI	GCATC	7 cut(s)	51, 547, 966, 1215, 1344, 1464, 1486
MaeI	CTAG	2 cut(s)	959, 1304
MaeII	ACGT	2 cut(s)	1005, 1320
MaeIII	GTNAC	4 cut(s)	220, 431, 947, 1006
MalI	GATC	3 cut(s)	617, 1200, 1508
MbiI	CCGCTC	1 cut(s)	1372
MboI	GATC	3 cut(s)	615, 1198, 1506
MboII	GAAGA	5 cut(s)	95, 98, 869, 1373, 1450
MfeI	CAATTG	1 cut(s)	810
MflI	RGATCY	1 cut(s)	1506
MhlI	GDGCHC	1 cut(s)	966
MlsI	TGGCCA	1 cut(s)	713
MluNI	TGGCCA	1 cut(s)	713
MlyI	GAGTC	2 cut(s)	1193, 1450
Mox20I	TGGCCA	1 cut(s)	713
Mph1103I	ATGCAT	2 cut(s)	375, 1479
MscI	TGGCCA	1 cut(s)	713
MseI	TTAA	3 cut(s)	783, 1229, 1517
Msp20I	TGGCCA	1 cut(s)	713
MspA1I	CMGCKG	1 cut(s)	110
MspI	CCGG	1 cut(s)	1275
MspR9I	CCNGG	2 cut(s)	1349, 1416
MunI	CAATTG	1 cut(s)	810
Mva1269I	GAATGC	4 cut(s)	217, 678, 888, 916
MvaI	CCWGG	2 cut(s)	1349, 1416
MvnI	CGCG	1 cut(s)	110
NdeI	CATATG	3 cut(s)	1041, 1270, 1527
NdeII	GATC	3 cut(s)	615, 1198, 1506
NlaIII	CATG	8 cut(s)	303, 373, 532, 732, 773, 1132, 1205, 1454
NlaIV	GGNNCC	1 cut(s)	132
NmuCI	GTSAC	2 cut(s)	220, 431
NsbI	TGCGCA	1 cut(s)	327
NsiI	ATGCAT	2 cut(s)	375, 1479
NspI	RCATGY	3 cut(s)	373, 532, 732
PasI	CCCWGGG	1 cut(s)	1348
PciI	ACATGT	1 cut(s)	528
PcsI	WCGNNNNNNNCGW	2 cut(s)	230, 401
PctI	GAATGC	4 cut(s)	217, 678, 888, 916
PfeI	GAWTC	4 cut(s)	160, 338, 792, 1307
PkrI	GCNGC	2 cut(s)	652, 1037
PleI	GAGTC	2 cut(s)	1192, 1449
PpsI	GAGTC	2 cut(s)	1192, 1449
Ppu21I	YACGTR	1 cut(s)	1321
PscI	ACATGT	1 cut(s)	528
Psp124BI	GAGCTC	1 cut(s)	966
Psp6I	CCWGG	2 cut(s)	1347, 1414
PspFI	CCCAGC	1 cut(s)	1151
PspGI	CCWGG	2 cut(s)	1347, 1414
PspN4I	GGNNCC	1 cut(s)	132
PstNI	CAGNNNCTG	1 cut(s)	426
PsuI	RGATCY	1 cut(s)	1506
RsaI	GTAC	2 cut(s)	630, 778
RsaNI	GTAC	2 cut(s)	629, 777
SacI	GAGCTC	1 cut(s)	966
SacII	CCGCGG	1 cut(s)	111
SaqAI	TTAA	3 cut(s)	783, 1229, 1517
SatI	GCNGC	2 cut(s)	651, 1036
Sau3AI	GATC	3 cut(s)	615, 1198, 1506
SchI	GAGTC	2 cut(s)	1193, 1450
ScrFI	CCNGG	2 cut(s)	1349, 1416
SduI	GDGCHC	1 cut(s)	966
SfaNI	GCATC	7 cut(s)	51, 547, 966, 1215, 1344, 1464, 1486
SfcI	CTRYAG	3 cut(s)	427, 667, 1420
Sfr303I	CCGCGG	1 cut(s)	111
SgrBI	CCGCGG	1 cut(s)	111
SmlI	CTYRAG	1 cut(s)	479
SmoI	CTYRAG	1 cut(s)	479
SsiI	CCGC	6 cut(s)	108, 110, 128, 173, 1357, 1372
SspMI	CTAG	2 cut(s)	959, 1304
SstI	GAGCTC	1 cut(s)	966
StyD4I	CCNGG	2 cut(s)	1347, 1414
StyI	CCWWGG	1 cut(s)	1106
TaaI	ACNGT	1 cut(s)	668
TaiI	ACGT	2 cut(s)	1008, 1323
TaqI	TCGA	2 cut(s)	1240, 1396
TatI	WGTACW	1 cut(s)	628
TfiI	GAWTC	4 cut(s)	160, 338, 792, 1307
Tru1I	TTAA	3 cut(s)	783, 1229, 1517
Tru9I	TTAA	3 cut(s)	783, 1229, 1517
TscAI	CASTG	1 cut(s)	427
TseFI	GTSAC	2 cut(s)	220, 431
TseI	GCWGC	2 cut(s)	650, 1035
Tsp45I	GTSAC	2 cut(s)	220, 431
TspGWI	ACGGA	1 cut(s)	410
TspRI	CASTG	1 cut(s)	427
XapI	RAATTY	7 cut(s)	137, 264, 289, 494, 803, 850, 1091
XbaI	TCTAGA	1 cut(s)	958
XceI	RCATGY	3 cut(s)	373, 532, 732
XspI	CTAG	2 cut(s)	959, 1304
Zsp2I	ATGCAT	2 cut(s)	375, 1479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.