Rorug01G0064900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001580
Physical Location & Seq
Forward (+)
10523932 .. 10524531
600 bp
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UTR
Exon/CDS
Intron
Rorug01G0064900.1

Sequence Viewer

Length: 600 bp
ATGGCCAGCAAGTACTCCTTTCCTCATGATCCCCTCGTCAACCTCACCATAATGGCTGCCATCAAGGCAAAAGACACTGCAGAAGAATTTTTCAAGCTTCAAAACTACGAGTTGGCGATCAAGCAGGCCATGGTAGCCAAACAATTCGACCCCGATTTGCGAGGTATCGATCATTTCATCCTTGCCTACCAGATTCATAAAGCCGTGTCGGAAAAGAAAGATTGGTATCATGTTCTTGGCTTCCAAGAGGTTACCGAGTATGCGACCATCAAGAAACAATACAAAGAGTTGGCACGCTTTGTACACCCTGACAAGAACAGCTCTATTGCTGCCGAAGGCGCTTTCAACCATGTCAAAGTGGCATGGGATTTTCTTTCTGACCCTGCCAAAAAGAAGGCCAACGACAAGTCGTTAAGTACATCGCGATTTCAAAGGGCGTCCTATGTCAAGAACACTTGCAAAAGGGCTTGCCCTCAGGCTAAGACCAGTACTAAAGATGATCGTGGTTTCTGTAAGAGAACAGTAAACATAATAAGGAAAACGAATACTGACCAAAACACGAGTACTACTCTGAGGTTGTCAGTACGTCGTGTTGCTTAG

Protein Analysis

199

Amino Acids

22.78

Weight (kDa)

9.71

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 74 - 134 2.8e-15 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 424
AclWI GGATC 1 cut(s) 23
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 437
AfaI GTAC 6 cut(s) 14, 303, 418, 490, 565, 585
AgsI TTSAA 4 cut(s) 94, 101, 346, 431
AluBI AGCT 2 cut(s) 97, 321
AluI AGCT 2 cut(s) 97, 321
AlwI GGATC 1 cut(s) 23
AoxI GGCC 3 cut(s) 3, 126, 396
ApeKI GCWGC 2 cut(s) 56, 329
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 474
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 559
BbvI GCAGC 2 cut(s) 43, 316
BccI CCATC 2 cut(s) 68, 275
BceAI ACGGC 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 342
BglI GCCNNNNNGGC 1 cut(s) 65
BisI GCNGC 2 cut(s) 57, 330
BlsI GCNGC 2 cut(s) 58, 331
BmcAI AGTACT 3 cut(s) 14, 490, 565
BplI GAGNNNNNCTC 2 cut(s) 553, 585
Bsa29I ATCGAT 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 129
Bse1I ACTGG 1 cut(s) 486
Bse21I CCTNAGG 1 cut(s) 474
Bse8I GATNNNNATC 1 cut(s) 225
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 177
BseJI GATNNNNATC 1 cut(s) 225
BseMII CTCAG 2 cut(s) 488, 563
BseNI ACTGG 1 cut(s) 486
BseXI GCAGC 2 cut(s) 43, 316
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 3 cut(s) 5, 128, 398
BshVI ATCGAT 1 cut(s) 168
BsnI GGCC 3 cut(s) 5, 128, 398
Bsp1407I TGTACA 1 cut(s) 301
Bsp143I GATC 4 cut(s) 28, 117, 169, 499
Bsp19I CCATGG 1 cut(s) 129
Bsp68I TCGCGA 1 cut(s) 424
BspANI GGCC 3 cut(s) 5, 128, 398
BspCNI CTCAG 2 cut(s) 487, 564
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 424
BspHI TCATGA 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 301
BsrI ACTGG 1 cut(s) 486
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 4 cut(s) 28, 117, 169, 499
BssNI GRCGYC 1 cut(s) 437
BssSI CACGAG 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 523
BstACI GRCGYC 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 301
BstC8I GCNNGC 4 cut(s) 7, 126, 295, 469
BstDEI CTNAG 4 cut(s) 474, 480, 572, 597
BstDSI CCRYGG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 250
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 424
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 4 cut(s) 31, 120, 172, 502
BstMBI GATC 4 cut(s) 28, 117, 169, 499
BstMWI GCNNNNNNNGC 3 cut(s) 65, 134, 338
BstPI GGTNACC 1 cut(s) 250
BstSFI CTRYAG 1 cut(s) 78
BstUI CGCG 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 43, 316
Bsu15I ATCGAT 1 cut(s) 168
Bsu36I CCTNAGG 1 cut(s) 474
BsuRI GGCC 3 cut(s) 5, 128, 398
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
BtuMI TCGCGA 1 cut(s) 424
Cac8I GCNNGC 4 cut(s) 7, 126, 295, 469
CciI TCATGA 1 cut(s) 25
CfoI GCGC 1 cut(s) 341
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 1 cut(s) 426
Csp6I GTAC 6 cut(s) 13, 302, 417, 489, 564, 584
CviAII CATG 5 cut(s) 26, 130, 230, 350, 363
CviQI GTAC 6 cut(s) 13, 302, 417, 489, 564, 584
DdeI CTNAG 4 cut(s) 474, 480, 572, 597
DpnI GATC 4 cut(s) 30, 119, 171, 501
DpnII GATC 4 cut(s) 28, 117, 169, 499
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 129
Eco81I CCTNAGG 1 cut(s) 474
Eco91I GGTNACC 1 cut(s) 250
EcoO65I GGTNACC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 5 cut(s) 29, 133, 233, 353, 366
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 5 cut(s) 25, 129, 229, 349, 362
Fnu4HI GCNGC 2 cut(s) 57, 330
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 57, 330
GlaI GCGC 1 cut(s) 340
GluI GCNGC 2 cut(s) 57, 330
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 3 cut(s) 5, 128, 398
HgaI GACGC 1 cut(s) 426
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 5 cut(s) 29, 133, 233, 353, 366
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 1 cut(s) 193
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 3 cut(s) 40, 304, 526
Hpy188I TCNGA 3 cut(s) 211, 379, 573
Hpy188III TCNNGA 4 cut(s) 26, 271, 423, 448
Hpy8I GTNNAC 3 cut(s) 40, 304, 526
Hpy99I CGWCG 1 cut(s) 591
HpyAV CCTTC 2 cut(s) 329, 388
HpyCH4III ACNGT 1 cut(s) 523
HpyCH4IV ACGT 1 cut(s) 586
HpyCH4V TGCA 2 cut(s) 80, 459
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 134, 338
HpyF3I CTNAG 4 cut(s) 474, 480, 572, 597
HpySE526I ACGT 1 cut(s) 586
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 5 cut(s) 29, 133, 233, 353, 366
HspAI GCGC 1 cut(s) 339
Kzo9I GATC 4 cut(s) 28, 117, 169, 499
LpnPI CCDG 7 cut(s) 19, 110, 203, 321, 396, 461, 499
Lsp1109I GCAGC 2 cut(s) 43, 316
MaeII ACGT 1 cut(s) 586
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 30, 119, 171, 501
MboI GATC 4 cut(s) 28, 117, 169, 499
MboII GAAGA 1 cut(s) 95
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 86, 143
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 7 cut(s) 33, 44, 53, 155, 241, 483, 567
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 413
MslI CAYNNNNRTG 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 5
MvnI CGCG 1 cut(s) 424
MwoI GCNNNNNNNGC 3 cut(s) 65, 134, 338
NcoI CCATGG 1 cut(s) 129
NdeII GATC 4 cut(s) 28, 117, 169, 499
NlaIII CATG 5 cut(s) 29, 133, 233, 353, 366
NruI TCGCGA 1 cut(s) 424
PagI TCATGA 1 cut(s) 25
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 2 cut(s) 58, 331
PspEI GGTNACC 1 cut(s) 250
PstI CTGCAG 1 cut(s) 82
RruI TCGCGA 1 cut(s) 424
RsaI GTAC 6 cut(s) 14, 303, 418, 490, 565, 585
RsaNI GTAC 6 cut(s) 13, 302, 417, 489, 564, 584
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 413
SatI GCNGC 2 cut(s) 57, 330
Sau3AI GATC 4 cut(s) 28, 117, 169, 499
ScaI AGTACT 3 cut(s) 14, 490, 565
SetI ASST 7 cut(s) 45, 99, 166, 252, 323, 578, 589
SfcI CTRYAG 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 2 cut(s) 86, 143
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 523
TaiI ACGT 1 cut(s) 589
TaqI TCGA 2 cut(s) 147, 168
TasI AATT 2 cut(s) 86, 143
TatI WGTACW 5 cut(s) 12, 301, 416, 488, 563
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 1 cut(s) 82
TseI GCWGC 2 cut(s) 56, 329
TspDTI ATGAA 2 cut(s) 166, 185
TspRI CASTG 1 cut(s) 82
XapI RAATTY 1 cut(s) 86
ZrmI AGTACT 3 cut(s) 14, 490, 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.