Rh2DG154200

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001580
Physical Location & Seq
Reverse (-)
12889261 .. 12895976
6716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG154200.1

Sequence Viewer

Length: 333 bp
ATGGAGTTAGGTTCACTAGAATCACAGTCTTACGTGCTATTGTCAAGCATCTATACTTCCCTGGGTAGGCGGGAAGATGTTGAGCGGGTAAGGAGGATGATGAAACTTCGAGGGGCCCAGCCACCATCTGGTGAGGTTCAAGCTGATAATGTTGAACCTGAAGCTATTCCAACAAAGAATGTTCAAGAAGATGTTCCAGCCCCTGATCCAGCTCCTGCTGATGTGCCTAATGTGTCTTCTCAAGCTCCAGCAAGCTCTGGATTTAAACAAAGAACATTCAAATCCCTTGCACAGAGGAAACCAAAGCCTCCAAAGCAGCCACAAGTTCCTTGA

Protein Analysis

110

Amino Acids

12.02

Weight (kDa)

9.1

Isoelectric Point (pI)

67.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 2 - 40 1.5e-08 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 128
AccBSI CCGCTC 1 cut(s) 85
AciI CCGC 2 cut(s) 70, 85
AclWI GGATC 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 66, 128
AgsI TTSAA 4 cut(s) 140, 155, 185, 280
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 5 cut(s) 143, 164, 212, 245, 255
AluI AGCT 5 cut(s) 143, 164, 212, 245, 255
AlwI GGATC 1 cut(s) 200
AlwNI CAGNNNCTG 2 cut(s) 203, 215
AoxI GGCC 1 cut(s) 114
ApaI GGGCCC 1 cut(s) 118
ApeKI GCWGC 1 cut(s) 316
Asp700I GAANNNNTTC 2 cut(s) 165, 192
AspS9I GGNCC 2 cut(s) 114, 115
AsuHPI GGTGA 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 328
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 62
BfaI CTAG 1 cut(s) 17
BisI GCNGC 1 cut(s) 317
BlsI GCNGC 1 cut(s) 318
Bme1390I CCNGG 1 cut(s) 62
BmgT120I GGNCC 2 cut(s) 114, 115
BmiI GGNNCC 2 cut(s) 115, 116
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 34
BsaJI CCNNGG 2 cut(s) 60, 61
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 128
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 60, 61
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 66, 128
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 1 cut(s) 328
BseYI CCCAGC 1 cut(s) 117
BshFI GGCC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 66, 128
BsnI GGCC 1 cut(s) 116
Bsp120I GGGCCC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 2 cut(s) 70, 85
BspANI GGCC 1 cut(s) 116
BspLI GGNNCC 2 cut(s) 115, 116
BspPI GGATC 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 60, 61
BssMI GATC 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 27
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 253
BstF5I GGATG 1 cut(s) 102
BstKTI GATC 1 cut(s) 208
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstNI CCWGG 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 228
BsuRI GGCC 1 cut(s) 116
BtsCI GGATG 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 253
CaiI CAGNNNCTG 2 cut(s) 203, 215
Cfr13I GGNCC 2 cut(s) 114, 115
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DraI TTTAAA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 118
FaiI YATR 1 cut(s) 54
FauI CCCGC 2 cut(s) 63, 78
Fnu4HI GCNGC 1 cut(s) 317
FokI GGATG 1 cut(s) 109
FriOI GRGCYC 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 317
FspBI CTAG 1 cut(s) 17
GluI GCNGC 1 cut(s) 317
GsaI CCCAGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 116
HinfI GANTC 1 cut(s) 20
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 14
Hpy188III TCNNGA 2 cut(s) 185, 258
Hpy8I GTNNAC 1 cut(s) 14
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpySE526I ACGT 1 cut(s) 33
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 217, 250
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 1 cut(s) 57
MaeI CTAG 1 cut(s) 17
MaeII ACGT 1 cut(s) 33
MalI GATC 1 cut(s) 207
MbiI CCGCTC 1 cut(s) 85
MboI GATC 1 cut(s) 205
MboII GAAGA 3 cut(s) 86, 200, 228
MhlI GDGCHC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 5 cut(s) 87, 104, 127, 288, 318
MroXI GAANNNNTTC 2 cut(s) 165, 192
MseI TTAA 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 313
NdeII GATC 1 cut(s) 205
NlaIV GGNNCC 2 cut(s) 115, 116
PasI CCCWGGG 1 cut(s) 61
PdmI GAANNNNTTC 2 cut(s) 165, 192
PfeI GAWTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 128
PkrI GCNGC 1 cut(s) 318
Ppu21I YACGTR 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 60
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 2 cut(s) 115, 116
PspOMI GGGCCC 1 cut(s) 114
PspPI GGNCC 2 cut(s) 114, 115
PstNI CAGNNNCTG 2 cut(s) 203, 215
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 1 cut(s) 317
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 2 cut(s) 114, 115
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 1 cut(s) 118
SetI ASST 9 cut(s) 13, 36, 138, 145, 160, 166, 214, 247, 257
SfaNI GCATC 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
SsiI CCGC 2 cut(s) 70, 85
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 109
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TseI GCWGC 1 cut(s) 316
TspDTI ATGAA 1 cut(s) 116
Van91I CCANNNNNTGG 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmnI GAANNNNTTC 2 cut(s) 165, 192
XspI CTAG 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.