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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 163 |
| Acc36I | ACCTGC | 1 cut(s) | 163 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 431 |
| AciI | CCGC | 1 cut(s) | 323 |
| AcsI | RAATTY | 1 cut(s) | 23 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 431 |
| AflIII | ACRYGT | 1 cut(s) | 91 |
| AgsI | TTSAA | 1 cut(s) | 329 |
| AjnI | CCWGG | 2 cut(s) | 66, 224 |
| AluBI | AGCT | 2 cut(s) | 260, 334 |
| AluI | AGCT | 2 cut(s) | 260, 334 |
| Alw26I | GTCTC | 1 cut(s) | 106 |
| ApoI | RAATTY | 1 cut(s) | 23 |
| AseI | ATTAAT | 1 cut(s) | 339 |
| AsuHPI | GGTGA | 2 cut(s) | 55, 144 |
| BbvCI | CCTCAGC | 1 cut(s) | 256 |
| BccI | CCATC | 4 cut(s) | 60, 310, 425, 433 |
| BciT130I | CCWGG | 2 cut(s) | 68, 226 |
| BcoDI | GTCTC | 1 cut(s) | 106 |
| BfaI | CTAG | 1 cut(s) | 417 |
| BfuAI | ACCTGC | 1 cut(s) | 163 |
| BlpI | GCTNAGC | 1 cut(s) | 261 |
| Bme1390I | CCNGG | 2 cut(s) | 68, 226 |
| BmrFI | CCNGG | 2 cut(s) | 68, 226 |
| BmsI | GCATC | 3 cut(s) | 193, 219, 254 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 103, 135 |
| Bpu10I | CCTNAGC | 1 cut(s) | 256 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 261 |
| BpuEI | CTTGAG | 3 cut(s) | 84, 157, 410 |
| BsaJI | CCNNGG | 2 cut(s) | 66, 190 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 431 |
| BseBI | CCWGG | 2 cut(s) | 68, 226 |
| BseDI | CCNNGG | 2 cut(s) | 66, 190 |
| BseGI | GGATG | 2 cut(s) | 52, 234 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 431 |
| BseMII | CTCAG | 1 cut(s) | 270 |
| BseRI | GAGGAG | 1 cut(s) | 244 |
| BslFI | GGGAC | 1 cut(s) | 80 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 431 |
| BsmAI | GTCTC | 1 cut(s) | 106 |
| BsmFI | GGGAC | 1 cut(s) | 80 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 261 |
| Bsp19I | CCATGG | 1 cut(s) | 190 |
| BspACI | CCGC | 1 cut(s) | 323 |
| BspCNI | CTCAG | 1 cut(s) | 269 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspMI | ACCTGC | 1 cut(s) | 163 |
| BspQI | GCTCTTC | 1 cut(s) | 291 |
| BssECI | CCNNGG | 2 cut(s) | 66, 190 |
| BssT1I | CCWWGG | 1 cut(s) | 190 |
| Bst2UI | CCWGG | 2 cut(s) | 68, 226 |
| Bst4CI | ACNGT | 3 cut(s) | 144, 242, 346 |
| Bst6I | CTCTTC | 2 cut(s) | 102, 291 |
| BstDEI | CTNAG | 3 cut(s) | 42, 256, 261 |
| BstDSI | CCRYGG | 1 cut(s) | 190 |
| BstF5I | GGATG | 2 cut(s) | 52, 234 |
| BstMAI | GTCTC | 1 cut(s) | 106 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 43, 298 |
| BstNI | CCWGG | 2 cut(s) | 68, 226 |
| BstNSI | RCATGY | 1 cut(s) | 95 |
| BstSCI | CCNGG | 2 cut(s) | 66, 224 |
| BtgI | CCRYGG | 1 cut(s) | 190 |
| BtsCI | GGATG | 2 cut(s) | 52, 234 |
| BveI | ACCTGC | 1 cut(s) | 163 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 9, 44 |
| CviAII | CATG | 3 cut(s) | 92, 191, 271 |
| CviJI | RGCY | 6 cut(s) | 11, 32, 46, 260, 334, 388 |
| CviKI_1 | RGCY | 6 cut(s) | 11, 32, 46, 260, 334, 388 |
| DdeI | CTNAG | 3 cut(s) | 42, 256, 261 |
| Eam1104I | CTCTTC | 2 cut(s) | 102, 291 |
| EarI | CTCTTC | 2 cut(s) | 102, 291 |
| Eco130I | CCWWGG | 1 cut(s) | 190 |
| EcoRII | CCWGG | 2 cut(s) | 66, 224 |
| EcoT14I | CCWWGG | 1 cut(s) | 190 |
| ErhI | CCWWGG | 1 cut(s) | 190 |
| FaeI | CATG | 3 cut(s) | 95, 194, 274 |
| FaiI | YATR | 6 cut(s) | 93, 187, 192, 272, 303, 362 |
| FaqI | GGGAC | 1 cut(s) | 80 |
| FatI | CATG | 3 cut(s) | 91, 190, 270 |
| FokI | GGATG | 2 cut(s) | 39, 241 |
| FspBI | CTAG | 1 cut(s) | 417 |
| Hin1II | CATG | 3 cut(s) | 95, 194, 274 |
| HinfI | GANTC | 3 cut(s) | 182, 410, 421 |
| HphI | GGTGA | 2 cut(s) | 55, 144 |
| Hpy188I | TCNGA | 4 cut(s) | 163, 238, 315, 430 |
| Hpy188III | TCNNGA | 3 cut(s) | 101, 200, 271 |
| Hpy99I | CGWCG | 1 cut(s) | 350 |
| HpyAV | CCTTC | 1 cut(s) | 48 |
| HpyCH4III | ACNGT | 3 cut(s) | 144, 242, 346 |
| HpyCH4V | TGCA | 3 cut(s) | 232, 245, 364 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 43, 298 |
| HpyF3I | CTNAG | 3 cut(s) | 42, 256, 261 |
| Hsp92II | CATG | 3 cut(s) | 95, 194, 274 |
| LguI | GCTCTTC | 1 cut(s) | 291 |
| LmnI | GCTCC | 1 cut(s) | 434 |
| LpnPI | CCDG | 7 cut(s) | 53, 80, 168, 211, 213, 238, 264 |
| LweI | GCATC | 3 cut(s) | 193, 219, 254 |
| MaeI | CTAG | 1 cut(s) | 417 |
| MboII | GAAGA | 4 cut(s) | 119, 308, 362, 419 |
| MluCI | AATT | 1 cut(s) | 23 |
| MmeI | TCCRAC | 1 cut(s) | 216 |
| MnlI | CCTC | 5 cut(s) | 57, 108, 169, 265, 313 |
| MseI | TTAA | 1 cut(s) | 339 |
| MspR9I | CCNGG | 2 cut(s) | 68, 226 |
| MvaI | CCWGG | 2 cut(s) | 68, 226 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 43, 298 |
| NcoI | CCATGG | 1 cut(s) | 190 |
| NlaIII | CATG | 3 cut(s) | 95, 194, 274 |
| NspI | RCATGY | 1 cut(s) | 95 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PaqCI | CACCTGC | 1 cut(s) | 163 |
| PciI | ACATGT | 1 cut(s) | 91 |
| PciSI | GCTCTTC | 1 cut(s) | 291 |
| PfeI | GAWTC | 3 cut(s) | 182, 410, 421 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 431 |
| PfoI | TCCNGGA | 1 cut(s) | 224 |
| PscI | ACATGT | 1 cut(s) | 91 |
| PshBI | ATTAAT | 1 cut(s) | 339 |
| Psp6I | CCWGG | 2 cut(s) | 66, 224 |
| PspGI | CCWGG | 2 cut(s) | 66, 224 |
| SapI | GCTCTTC | 1 cut(s) | 291 |
| SaqAI | TTAA | 1 cut(s) | 339 |
| ScrFI | CCNGG | 2 cut(s) | 68, 226 |
| SetI | ASST | 4 cut(s) | 19, 157, 262, 336 |
| SfaNI | GCATC | 3 cut(s) | 193, 219, 254 |
| SmlI | CTYRAG | 3 cut(s) | 99, 172, 389 |
| SmoI | CTYRAG | 3 cut(s) | 99, 172, 389 |
| Sse9I | AATT | 1 cut(s) | 23 |
| SsiI | CCGC | 1 cut(s) | 323 |
| SspMI | CTAG | 1 cut(s) | 417 |
| StyD4I | CCNGG | 2 cut(s) | 66, 224 |
| StyI | CCWWGG | 1 cut(s) | 190 |
| TaaI | ACNGT | 3 cut(s) | 144, 242, 346 |
| TaqI | TCGA | 2 cut(s) | 294, 348 |
| TasI | AATT | 1 cut(s) | 23 |
| TfiI | GAWTC | 3 cut(s) | 182, 410, 421 |
| Tru1I | TTAA | 1 cut(s) | 339 |
| Tru9I | TTAA | 1 cut(s) | 339 |
| TspDTI | ATGAA | 2 cut(s) | 120, 420 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 431 |
| VspI | ATTAAT | 1 cut(s) | 339 |
| XapI | RAATTY | 1 cut(s) | 23 |
| XceI | RCATGY | 1 cut(s) | 95 |
| XspI | CTAG | 1 cut(s) | 417 |