Rroxscaffold_3G00228150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007494
Physical Location & Seq
Forward (+)
12500525 .. 12514717
14193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228150.1

Sequence Viewer

Length: 513 bp
ATGCGATCGACGGTGCGTCTCACCCTTAGCAATGCAAGTCATGATGACCGGGGCAACCACGCTAGAAGAGCAGTAGCTAATCCGATGGAGGCGGTTCAAAAGTTCACCAATACCTTCGAGGAAAAGGATATGCAGATTGCTCAACTTTGGAGTAGGCTTGAGAAGCAAAATGAAGAGGAATCTGCTAGGGTTCCGTACAAGGATGACAAAGACAAACAAGAAGAAACTTCAAAGGCAAATGACAAGGACGGTGAAGGCCAACTTGGTTCTTTGTCCGTCCAAAGACCGCAAGACATGATTAACAACTCCATTAGAGCTCAATATGGAGGTATCTCTTGTGATTCTCTCACATACTCCAAGCCATACACAAAAAGAGTAGATAGCCCGAGGATGCCATATGGGTACCGGCGCCAAGTTTCAGCAATTCGAGGGGAAGGGCAATCCAAAACAACATATTGCTCATTTTGTCGAGACTTTCAACAATGCTGGAACATAGGGAGATTATCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotrans_gag

Protein Analysis

170

Amino Acids

19.5

Weight (kDa)

8.8

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 2 cut(s) 402, 408
AciI CCGC 2 cut(s) 92, 287
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 2 cut(s) 197, 404
AgsI TTSAA 3 cut(s) 98, 231, 479
AhdI GACNNNNNGTC 1 cut(s) 15
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 2 cut(s) 77, 317
AluI AGCT 2 cut(s) 77, 317
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 2 cut(s) 23, 465
Ama87I CYCGRG 1 cut(s) 385
AoxI GGCC 1 cut(s) 256
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 1 cut(s) 411
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 3 cut(s) 13, 97, 263
AvaI CYCGRG 1 cut(s) 385
BanI GGYRCC 2 cut(s) 402, 408
BanII GRGCYC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 319
BccI CCATC 1 cut(s) 79
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 2 cut(s) 23, 465
BfaI CTAG 2 cut(s) 63, 186
BfoI RGCGCY 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 50
BmeRI GACNNNNNGTC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 385
BmiI GGNNCC 3 cut(s) 192, 404, 410
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 49, 386
Bse118I RCCGGY 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 37
BseDI CCNNGG 2 cut(s) 49, 386
BseGI GGATG 2 cut(s) 208, 396
BseMI GCAATG 1 cut(s) 37
Bsh1285I CGRYCG 1 cut(s) 8
BshFI GGCC 1 cut(s) 258
BshNI GGYRCC 2 cut(s) 402, 408
BsiEI CGRYCG 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 385
BsiSI CCGG 2 cut(s) 49, 406
BsmAI GTCTC 2 cut(s) 23, 465
BsmBI CGTCTC 1 cut(s) 23
BsnI GGCC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 1 cut(s) 5
BspACI CCGC 2 cut(s) 92, 287
BspANI GGCC 1 cut(s) 258
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 3 cut(s) 192, 404, 410
BspQI GCTCTTC 1 cut(s) 61
BspT107I GGYRCC 2 cut(s) 402, 408
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 405
BssECI CCNNGG 2 cut(s) 49, 386
BssMI GATC 1 cut(s) 5
BssNI GRCGYC 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 13, 251
Bst6I CTCTTC 2 cut(s) 61, 168
BstACI GRCGYC 1 cut(s) 409
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 2 cut(s) 208, 396
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 1 cut(s) 8
BstMAI GTCTC 2 cut(s) 23, 465
BstMBI GATC 1 cut(s) 5
BstMCI CGRYCG 1 cut(s) 8
BstMWI GCNNNNNNNGC 2 cut(s) 68, 163
BstSCI CCNGG 1 cut(s) 48
BsuRI GGCC 1 cut(s) 258
BtsCI GGATG 2 cut(s) 208, 396
CciI TCATGA 1 cut(s) 40
CfoI GCGC 1 cut(s) 411
Cfr10I RCCGGY 1 cut(s) 405
CseI GACGC 1 cut(s) 5
Csp6I GTAC 2 cut(s) 196, 403
CviAII CATG 2 cut(s) 41, 295
CviJI RGCY 6 cut(s) 77, 157, 258, 317, 361, 384
CviKI_1 RGCY 6 cut(s) 77, 157, 258, 317, 361, 384
CviQI GTAC 2 cut(s) 196, 403
DdeI CTNAG 1 cut(s) 26
DinI GGCGCC 1 cut(s) 410
DpnI GATC 1 cut(s) 7
DpnII GATC 1 cut(s) 5
DriI GACNNNNNGTC 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 61, 168
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 2 cut(s) 61, 168
Ecl136II GAGCTC 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 319
Eco53kI GAGCTC 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 385
EcoICRI GAGCTC 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 319
EgeI GGCGCC 1 cut(s) 410
EheI GGCGCC 1 cut(s) 410
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 2 cut(s) 44, 298
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FatI CATG 2 cut(s) 40, 294
FauNDI CATATG 1 cut(s) 397
FokI GGATG 2 cut(s) 215, 403
FriOI GRGCYC 1 cut(s) 319
FspBI CTAG 2 cut(s) 63, 186
GlaI GCGC 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 1 cut(s) 258
HapII CCGG 2 cut(s) 49, 406
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 411
Hin1I GRCGYC 1 cut(s) 409
Hin1II CATG 2 cut(s) 44, 298
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HinfI GANTC 2 cut(s) 179, 341
HpaII CCGG 2 cut(s) 49, 406
HphI GGTGA 3 cut(s) 13, 97, 263
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 2 cut(s) 41, 470
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 124, 248, 428
HpyCH4III ACNGT 2 cut(s) 13, 251
HpyCH4V TGCA 2 cut(s) 35, 133
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 163
HpyF3I CTNAG 1 cut(s) 26
Hsp92I GRCGYC 1 cut(s) 409
Hsp92II CATG 2 cut(s) 44, 298
HspAI GCGC 1 cut(s) 409
KasI GGCGCC 1 cut(s) 408
KpnI GGTACC 1 cut(s) 406
Kzo9I GATC 1 cut(s) 5
LguI GCTCTTC 1 cut(s) 61
LpnPI CCDG 3 cut(s) 62, 419, 472
LweI GCATC 1 cut(s) 381
MaeI CTAG 2 cut(s) 63, 186
MalI GATC 1 cut(s) 7
MboI GATC 1 cut(s) 5
MboII GAAGA 3 cut(s) 78, 185, 233
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 1 cut(s) 423
Mly113I GGCGCC 1 cut(s) 409
MnlI CCTC 6 cut(s) 82, 112, 169, 320, 381, 422
MseI TTAA 2 cut(s) 300, 511
MspI CCGG 2 cut(s) 49, 406
MspR9I CCNGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 68, 163
NarI GGCGCC 1 cut(s) 409
NciI CCSGG 1 cut(s) 50
NdeI CATATG 1 cut(s) 397
NdeII GATC 1 cut(s) 5
NlaIII CATG 2 cut(s) 44, 298
NlaIV GGNNCC 3 cut(s) 192, 404, 410
PagI TCATGA 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 61
PfeI GAWTC 2 cut(s) 179, 341
Ple19I CGATCG 1 cut(s) 8
PluTI GGCGCC 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 319
PspN4I GGNNCC 3 cut(s) 192, 404, 410
PvuI CGATCG 1 cut(s) 8
RsaI GTAC 2 cut(s) 197, 404
RsaNI GTAC 2 cut(s) 196, 403
SacI GAGCTC 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 2 cut(s) 300, 511
Sau3AI GATC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 1 cut(s) 319
SetI ASST 4 cut(s) 79, 116, 319, 331
SfaNI GCATC 1 cut(s) 381
SfoI GGCGCC 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 1 cut(s) 423
SsiI CCGC 2 cut(s) 92, 287
SspDI GGCGCC 1 cut(s) 408
SspMI CTAG 2 cut(s) 63, 186
SstI GAGCTC 1 cut(s) 319
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 13, 251
TaqI TCGA 4 cut(s) 8, 117, 427, 469
TasI AATT 1 cut(s) 423
TfiI GAWTC 2 cut(s) 179, 341
Tru1I TTAA 2 cut(s) 300, 511
Tru9I TTAA 2 cut(s) 300, 511
TspDTI ATGAA 1 cut(s) 186
TspGWI ACGGA 2 cut(s) 183, 265
XspI CTAG 2 cut(s) 63, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.