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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 259 |
| Acc36I | ACCTGC | 1 cut(s) | 259 |
| AciI | CCGC | 1 cut(s) | 179 |
| AclWI | GGATC | 1 cut(s) | 286 |
| AcuI | CTGAAG | 1 cut(s) | 324 |
| AfaI | GTAC | 1 cut(s) | 284 |
| AgsI | TTSAA | 1 cut(s) | 425 |
| AluBI | AGCT | 3 cut(s) | 215, 404, 430 |
| AluI | AGCT | 3 cut(s) | 215, 404, 430 |
| Alw26I | GTCTC | 2 cut(s) | 202, 348 |
| AlwI | GGATC | 1 cut(s) | 286 |
| AoxI | GGCC | 1 cut(s) | 51 |
| AsuHPI | GGTGA | 4 cut(s) | 151, 240, 338, 424 |
| BbvCI | CCTCAGC | 1 cut(s) | 352 |
| BccI | CCATC | 3 cut(s) | 32, 329, 406 |
| BceAI | ACGGC | 1 cut(s) | 66 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 58, 92, 176, 210 |
| BclI | TGATCA | 1 cut(s) | 262 |
| BcoDI | GTCTC | 2 cut(s) | 202, 348 |
| BfaI | CTAG | 1 cut(s) | 164 |
| BfuAI | ACCTGC | 1 cut(s) | 259 |
| BmsI | GCATC | 1 cut(s) | 315 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 199, 231 |
| Bpu10I | CCTNAGC | 1 cut(s) | 352 |
| BpuEI | CTTGAG | 1 cut(s) | 253 |
| BsaJI | CCNNGG | 1 cut(s) | 230 |
| Bse3DI | GCAATG | 1 cut(s) | 363 |
| BseDI | CCNNGG | 1 cut(s) | 230 |
| BseGI | GGATG | 2 cut(s) | 24, 330 |
| BseMI | GCAATG | 1 cut(s) | 363 |
| BseMII | CTCAG | 1 cut(s) | 366 |
| Bsh1285I | CGRYCG | 1 cut(s) | 242 |
| BshFI | GGCC | 1 cut(s) | 53 |
| BsiEI | CGRYCG | 1 cut(s) | 242 |
| BslFI | GGGAC | 1 cut(s) | 176 |
| BsmAI | GTCTC | 2 cut(s) | 202, 348 |
| BsmBI | CGTCTC | 1 cut(s) | 348 |
| BsmFI | GGGAC | 1 cut(s) | 176 |
| BsnI | GGCC | 1 cut(s) | 53 |
| Bsp143I | GATC | 3 cut(s) | 239, 262, 278 |
| BspACI | CCGC | 1 cut(s) | 179 |
| BspANI | GGCC | 1 cut(s) | 53 |
| BspCNI | CTCAG | 1 cut(s) | 365 |
| BspMI | ACCTGC | 1 cut(s) | 259 |
| BspPI | GGATC | 1 cut(s) | 286 |
| BspQI | GCTCTTC | 1 cut(s) | 387 |
| BsrDI | GCAATG | 1 cut(s) | 363 |
| BssECI | CCNNGG | 1 cut(s) | 230 |
| BssMI | GATC | 3 cut(s) | 239, 262, 278 |
| BssT1I | CCWWGG | 1 cut(s) | 230 |
| Bst4CI | ACNGT | 1 cut(s) | 442 |
| Bst6I | CTCTTC | 2 cut(s) | 198, 387 |
| BstDEI | CTNAG | 3 cut(s) | 138, 256, 352 |
| BstF5I | GGATG | 2 cut(s) | 24, 330 |
| BstKTI | GATC | 3 cut(s) | 242, 265, 281 |
| BstMAI | GTCTC | 2 cut(s) | 202, 348 |
| BstMBI | GATC | 3 cut(s) | 239, 262, 278 |
| BstMCI | CGRYCG | 1 cut(s) | 242 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 139, 394 |
| BstNSI | RCATGY | 1 cut(s) | 191 |
| BsuRI | GGCC | 1 cut(s) | 53 |
| BtsCI | GGATG | 2 cut(s) | 24, 330 |
| BveI | ACCTGC | 1 cut(s) | 259 |
| CseI | GACGC | 1 cut(s) | 330 |
| Csp6I | GTAC | 1 cut(s) | 283 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 103, 138 |
| CviAII | CATG | 3 cut(s) | 97, 188, 299 |
| CviJI | RGCY | 8 cut(s) | 5, 23, 53, 112, 142, 215, 404, 430 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 23, 53, 112, 142, 215, 404, 430 |
| CviQI | GTAC | 1 cut(s) | 283 |
| DdeI | CTNAG | 3 cut(s) | 138, 256, 352 |
| DpnI | GATC | 3 cut(s) | 241, 264, 280 |
| DpnII | GATC | 3 cut(s) | 239, 262, 278 |
| Eam1104I | CTCTTC | 2 cut(s) | 198, 387 |
| EarI | CTCTTC | 2 cut(s) | 198, 387 |
| Eco130I | CCWWGG | 1 cut(s) | 230 |
| Eco57I | CTGAAG | 1 cut(s) | 324 |
| EcoT14I | CCWWGG | 1 cut(s) | 230 |
| ErhI | CCWWGG | 1 cut(s) | 230 |
| Esp3I | CGTCTC | 1 cut(s) | 348 |
| FaeI | CATG | 3 cut(s) | 100, 191, 302 |
| FaiI | YATR | 6 cut(s) | 98, 189, 300, 368, 438, 458 |
| FaqI | GGGAC | 1 cut(s) | 176 |
| FatI | CATG | 3 cut(s) | 96, 187, 298 |
| FauI | CCCGC | 1 cut(s) | 172 |
| FbaI | TGATCA | 1 cut(s) | 262 |
| FokI | GGATG | 2 cut(s) | 11, 337 |
| FspBI | CTAG | 1 cut(s) | 164 |
| HaeIII | GGCC | 1 cut(s) | 53 |
| HgaI | GACGC | 1 cut(s) | 330 |
| Hin1II | CATG | 3 cut(s) | 100, 191, 302 |
| HinfI | GANTC | 1 cut(s) | 421 |
| HphI | GGTGA | 4 cut(s) | 151, 240, 338, 424 |
| Hpy188I | TCNGA | 2 cut(s) | 334, 411 |
| Hpy188III | TCNNGA | 1 cut(s) | 28 |
| HpyAV | CCTTC | 1 cut(s) | 144 |
| HpyCH4III | ACNGT | 1 cut(s) | 442 |
| HpyCH4V | TGCA | 3 cut(s) | 328, 361, 460 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 139, 394 |
| HpyF3I | CTNAG | 3 cut(s) | 138, 256, 352 |
| Hsp92II | CATG | 3 cut(s) | 100, 191, 302 |
| Ksp22I | TGATCA | 1 cut(s) | 262 |
| Kzo9I | GATC | 3 cut(s) | 239, 262, 278 |
| LguI | GCTCTTC | 1 cut(s) | 387 |
| LmnI | GCTCC | 2 cut(s) | 220, 377 |
| LpnPI | CCDG | 2 cut(s) | 41, 264 |
| LweI | GCATC | 1 cut(s) | 315 |
| MaeI | CTAG | 1 cut(s) | 164 |
| MaeIII | GTNAC | 1 cut(s) | 85 |
| MalI | GATC | 3 cut(s) | 241, 264, 280 |
| MboI | GATC | 3 cut(s) | 239, 262, 278 |
| MboII | GAAGA | 4 cut(s) | 215, 317, 404, 458 |
| MnlI | CCTC | 4 cut(s) | 64, 204, 361, 409 |
| MseI | TTAA | 1 cut(s) | 469 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 139, 394 |
| NdeII | GATC | 3 cut(s) | 239, 262, 278 |
| NlaIII | CATG | 3 cut(s) | 100, 191, 302 |
| NspI | RCATGY | 1 cut(s) | 191 |
| PaqCI | CACCTGC | 1 cut(s) | 259 |
| PciSI | GCTCTTC | 1 cut(s) | 387 |
| PfeI | GAWTC | 1 cut(s) | 421 |
| Ple19I | CGATCG | 1 cut(s) | 242 |
| PvuI | CGATCG | 1 cut(s) | 242 |
| RsaI | GTAC | 1 cut(s) | 284 |
| RsaNI | GTAC | 1 cut(s) | 283 |
| SapI | GCTCTTC | 1 cut(s) | 387 |
| SaqAI | TTAA | 1 cut(s) | 469 |
| Sau3AI | GATC | 3 cut(s) | 239, 262, 278 |
| SetI | ASST | 5 cut(s) | 108, 217, 253, 406, 432 |
| SfaNI | GCATC | 1 cut(s) | 315 |
| SmlI | CTYRAG | 2 cut(s) | 18, 268 |
| SmoI | CTYRAG | 2 cut(s) | 18, 268 |
| SsiI | CCGC | 1 cut(s) | 179 |
| SspMI | CTAG | 1 cut(s) | 164 |
| StyI | CCWWGG | 1 cut(s) | 230 |
| TaaI | ACNGT | 1 cut(s) | 442 |
| TaqI | TCGA | 3 cut(s) | 196, 390, 444 |
| TaqII | GACCGA | 1 cut(s) | 68 |
| TfiI | GAWTC | 1 cut(s) | 421 |
| Tru1I | TTAA | 1 cut(s) | 469 |
| Tru9I | TTAA | 1 cut(s) | 469 |
| TspDTI | ATGAA | 1 cut(s) | 287 |
| XceI | RCATGY | 1 cut(s) | 191 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 121 |
| XspI | CTAG | 1 cut(s) | 164 |