Rmu_co8145310.1_g000001

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8145310.1
OG0005206
Physical Location & Seq
Reverse (-)
2 .. 421
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8145310.1_g000001.1.cds

Sequence Viewer

Length: 270 bp
atgcctctagctagtagcaccatacagagtaatagtatggctcttcgatggtttctccttctcaacctcatttgcagtattctactgcttgtcgatgttactcattcagctgctcaggatgcccgaaaggcttatgttgtgtatatgggcggcaagccaaagaacggggtacccatattacctgatcttcatgtgaatatgctacaagacgtagttgacagtagcaacattgatattgcacatgaagatctgcttcttcacagctacaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

90

Amino Acids

9.94

Weight (kDa)

5.99

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 169
AciI CCGC 1 cut(s) 150
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 164
AluBI AGCT 3 cut(s) 11, 110, 264
AluI AGCT 3 cut(s) 11, 110, 264
ApeKI GCWGC 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 97
BccI CCATC 1 cut(s) 42
BfaI CTAG 2 cut(s) 8, 12
BglI GCCNNNNNGGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 247
BisI GCNGC 2 cut(s) 111, 151
BlsI GCNGC 2 cut(s) 112, 152
BmiI GGNNCC 1 cut(s) 171
BmsI GCATC 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMII CTCAG 1 cut(s) 128
BseXI GCAGC 1 cut(s) 97
BshNI GGYRCC 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 164
Bsp143I GATC 2 cut(s) 184, 247
BspACI CCGC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 171
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 169
BssMI GATC 2 cut(s) 184, 247
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 2 cut(s) 187, 250
BstMBI GATC 2 cut(s) 184, 247
BstMWI GCNNNNNNNGC 2 cut(s) 119, 128
BstV1I GCAGC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BtsCI GGATG 1 cut(s) 124
Cac8I GCNNGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 170
CviAII CATG 2 cut(s) 191, 242
CviJI RGCY 6 cut(s) 11, 41, 110, 131, 157, 264
CviKI_1 RGCY 6 cut(s) 11, 41, 110, 131, 157, 264
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 1 cut(s) 114
DpnI GATC 2 cut(s) 186, 249
DpnII GATC 2 cut(s) 184, 247
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
FaeI CATG 2 cut(s) 194, 245
FaiI YATR 9 cut(s) 23, 38, 135, 144, 146, 176, 192, 200, 243
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 2 cut(s) 190, 241
Fnu4HI GCNGC 2 cut(s) 111, 151
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 2 cut(s) 111, 151
FspBI CTAG 2 cut(s) 8, 12
GluI GCNGC 2 cut(s) 111, 151
Hin1II CATG 2 cut(s) 194, 245
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 217
Hpy188III TCNNGA 1 cut(s) 116
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 75, 239
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 128
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 2 cut(s) 194, 245
KpnI GGTACC 1 cut(s) 173
Kzo9I GATC 2 cut(s) 184, 247
LguI GCTCTTC 1 cut(s) 48
LpnPI CCDG 2 cut(s) 101, 195
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 1 cut(s) 109
MaeI CTAG 2 cut(s) 8, 12
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 186, 249
MboI GATC 2 cut(s) 184, 247
MboII GAAGA 4 cut(s) 35, 179, 248, 257
MflI RGATCY 1 cut(s) 247
MnlI CCTC 2 cut(s) 15, 77
MspA1I CMGCKG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 119, 128
NdeII GATC 2 cut(s) 184, 247
NlaIII CATG 2 cut(s) 194, 245
NlaIV GGNNCC 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 48
PkrI GCNGC 2 cut(s) 112, 152
PspN4I GGNNCC 1 cut(s) 171
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 48
SatI GCNGC 2 cut(s) 111, 151
Sau3AI GATC 2 cut(s) 184, 247
SetI ASST 6 cut(s) 13, 69, 112, 184, 213, 266
SfaNI GCATC 1 cut(s) 109
SsiI CCGC 1 cut(s) 150
SspMI CTAG 2 cut(s) 8, 12
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 46, 93
TauI GCSGC 1 cut(s) 153
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 179, 258
XspI CTAG 2 cut(s) 8, 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.