Rroxscaffold_4G00309930

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005206
Physical Location & Seq
Forward (+)
31784480 .. 31788488
4009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309930.1

Sequence Viewer

Length: 291 bp
ATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACTTGATCTTCATGTGAATATTCTAAGAGACGTAGTTGACAACAACATTAATAATGATATTTCACATGAAGCTCTTCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCATAGAACAAGAAGCACAAAAACTGGCTGTAAAATTATCGGAGCACGGTATTACCGTCATTTGGGATCCTTTGAAGAAGAACTTGAGTTTCCAAGAGACTCTGAAGGTCATGGAACCCATTACACATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

96

Amino Acids

10.89

Weight (kDa)

6.27

Isoelectric Point (pI)

35.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 16 - 62 8e-07 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AclWI GGATC 2 cut(s) 218, 231
AcuI CTGAAG 1 cut(s) 281
AfiI CCNNNNNNNGG 2 cut(s) 20, 219
AgsI TTSAA 1 cut(s) 232
AluBI AGCT 2 cut(s) 107, 123
AluI AGCT 2 cut(s) 107, 123
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 57, 248
AlwI GGATC 2 cut(s) 218, 231
ApeKI GCWGC 1 cut(s) 149
AseI ATTAAT 1 cut(s) 84
Asp700I GAANNNNTTC 1 cut(s) 108
BamHI GGATCC 1 cut(s) 223
BanI GGYRCC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 57, 248
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
BmiI GGNNCC 3 cut(s) 27, 225, 273
BpuEI CTTGAG 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 219
Bse1I ACTGG 2 cut(s) 29, 186
Bse3DI GCAATG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 139
BseLI CCNNNNNNNGG 2 cut(s) 20, 219
BseMI GCAATG 1 cut(s) 159
BseNI ACTGG 2 cut(s) 29, 186
BseXI GCAGC 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 20, 219
BsmAI GTCTC 2 cut(s) 57, 248
BsmBI CGTCTC 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 2 cut(s) 40, 223
Bsp19I CCATGG 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 27, 225, 273
BspPI GGATC 2 cut(s) 218, 231
BspQI GCTCTTC 1 cut(s) 114
BspT107I GGYRCC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 2 cut(s) 29, 186
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 2 cut(s) 40, 223
BssT1I CCWWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 206, 214
Bst6I CTCTTC 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 139
BstKTI GATC 2 cut(s) 43, 226
BstMAI GTCTC 2 cut(s) 57, 248
BstMBI GATC 2 cut(s) 40, 223
BstV1I GCAGC 1 cut(s) 136
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 139
CviAII CATG 4 cut(s) 47, 101, 140, 268
CviJI RGCY 4 cut(s) 13, 107, 123, 185
CviKI_1 RGCY 4 cut(s) 13, 107, 123, 185
DdeI CTNAG 1 cut(s) 59
DpnI GATC 2 cut(s) 42, 225
DpnII GATC 2 cut(s) 40, 223
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 281
EcoT14I CCWWGG 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 4 cut(s) 50, 104, 143, 271
FaiI YATR 5 cut(s) 48, 102, 141, 161, 269
FalI AAGNNNNNCTT 4 cut(s) 96, 128, 224, 256
FatI CATG 4 cut(s) 46, 100, 139, 267
Fnu4HI GCNGC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
Hin1II CATG 4 cut(s) 50, 104, 143, 271
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 199, 261
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 2 cut(s) 206, 214
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 1 cut(s) 152
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 4 cut(s) 50, 104, 143, 271
Kzo9I GATC 2 cut(s) 40, 223
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 2 cut(s) 42, 167
Lsp1109I GCAGC 1 cut(s) 136
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 42, 225
MboI GATC 2 cut(s) 40, 223
MboII GAAGA 6 cut(s) 35, 101, 104, 107, 244, 247
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 191
MlyI GAGTC 1 cut(s) 250
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 1 cut(s) 84
NcoI CCATGG 1 cut(s) 139
NdeII GATC 2 cut(s) 40, 223
NlaIII CATG 4 cut(s) 50, 104, 143, 271
NlaIV GGNNCC 3 cut(s) 27, 225, 273
PciSI GCTCTTC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 108
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PshBI ATTAAT 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 27, 225, 273
PsuI RGATCY 1 cut(s) 223
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 84
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 40, 223
SchI GAGTC 1 cut(s) 250
SduI GDGCHC 1 cut(s) 204
SetI ASST 4 cut(s) 69, 109, 125, 267
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 1 cut(s) 191
SspI AATATT 1 cut(s) 55
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 206, 214
TaiI ACGT 1 cut(s) 69
TasI AATT 1 cut(s) 191
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 35, 117
VspI ATTAAT 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.