Rh1CG174700

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0005206
Physical Location & Seq
Reverse (-)
38486279 .. 38487152
874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG174700.1

Sequence Viewer

Length: 582 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTAGCAGTATTTTACTTCTTGTTCATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTAAGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTTCTTCTTCATAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTAGCTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAATGAGCGTCAAAACAACAAAGTCATGGAACATCCTTGAGTTTCCAGAAACAGTTAAAAGAAGCAACCTTGAAAAAGATATCATTATCGGTGTGATCGACACTGGAATTTGGCCAGAATCGCACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAAGTATGCTAATGAATTTATCCATATAAGACACTCATCATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

193

Amino Acids

21.7

Weight (kDa)

8.5

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 7.2e-16 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 1 cut(s) 495
AcoI YGGCCR 1 cut(s) 443
AcsI RAATTY 2 cut(s) 438, 551
AfiI CCNNNNNNNGG 2 cut(s) 128, 495
AgsI TTSAA 1 cut(s) 404
AluBI AGCT 5 cut(s) 74, 215, 231, 293, 459
AluI AGCT 5 cut(s) 74, 215, 231, 293, 459
Alw26I GTCTC 1 cut(s) 165
AoxI GGCC 1 cut(s) 443
ApeKI GCWGC 2 cut(s) 74, 257
ApoI RAATTY 2 cut(s) 438, 551
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 479
AvaII GGWCC 1 cut(s) 479
BalI TGGCCA 1 cut(s) 445
BanI GGYRCC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 310
BbvI GCAGC 2 cut(s) 61, 244
BccI CCATC 2 cut(s) 6, 296
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 290
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 2 cut(s) 75, 258
BlsI GCNGC 2 cut(s) 76, 259
Bme18I GGWCC 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 479
BmiI GGNNCC 1 cut(s) 135
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 247
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 495
Bse1I ACTGG 2 cut(s) 137, 439
Bse3DI GCAATG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 3 cut(s) 88, 307, 363
BseLI CCNNNNNNNGG 2 cut(s) 128, 495
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 3 cut(s) 40, 84, 92
BseNI ACTGG 2 cut(s) 137, 439
BseXI GCAGC 2 cut(s) 61, 244
BshFI GGCC 1 cut(s) 445
BshNI GGYRCC 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 128, 495
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 1 cut(s) 445
Bsp143I GATC 2 cut(s) 148, 426
Bsp19I CCATGG 1 cut(s) 247
BspANI GGCC 1 cut(s) 445
BspCNI CTCAG 3 cut(s) 39, 83, 91
BspLI GGNNCC 1 cut(s) 135
BspQI GCTCTTC 2 cut(s) 12, 222
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 267
BsrI ACTGG 2 cut(s) 137, 439
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 2 cut(s) 148, 426
BssT1I CCWWGG 1 cut(s) 247
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 2 cut(s) 12, 222
BstDEI CTNAG 4 cut(s) 26, 70, 78, 167
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 3 cut(s) 88, 307, 363
BstKTI GATC 2 cut(s) 151, 429
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 148, 426
BstMWI GCNNNNNNNGC 5 cut(s) 36, 83, 92, 212, 451
BstV1I GCAGC 2 cut(s) 61, 244
BstV2I GAAGAC 1 cut(s) 310
BsuRI GGCC 1 cut(s) 445
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 3 cut(s) 88, 307, 363
BtsIMutI CAGTG 1 cut(s) 432
Cfr13I GGNCC 1 cut(s) 479
CseI GACGC 1 cut(s) 328
CviAII CATG 5 cut(s) 59, 155, 209, 248, 357
DdeI CTNAG 4 cut(s) 26, 70, 78, 167
DpnI GATC 2 cut(s) 150, 428
DpnII GATC 2 cut(s) 148, 426
EaeI YGGCCR 1 cut(s) 443
Eam1104I CTCTTC 2 cut(s) 12, 222
EarI CTCTTC 2 cut(s) 12, 222
Eco130I CCWWGG 1 cut(s) 247
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 479
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 247
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 5 cut(s) 62, 158, 212, 251, 360
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 5 cut(s) 58, 154, 208, 247, 356
FauNDI CATATG 1 cut(s) 506
Fnu4HI GCNGC 2 cut(s) 75, 258
FokI GGATG 3 cut(s) 95, 314, 350
Fsp4HI GCNGC 2 cut(s) 75, 258
FspBI CTAG 1 cut(s) 290
GluI GCNGC 2 cut(s) 75, 258
HaeIII GGCC 1 cut(s) 445
HgaI GACGC 1 cut(s) 328
Hin1II CATG 5 cut(s) 62, 158, 212, 251, 360
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 181
Hpy188III TCNNGA 2 cut(s) 80, 377
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 2 cut(s) 206, 260
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 83, 92, 212, 451
HpyF3I CTNAG 4 cut(s) 26, 70, 78, 167
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 5 cut(s) 62, 158, 212, 251, 360
Kzo9I GATC 2 cut(s) 148, 426
LguI GCTCTTC 2 cut(s) 12, 222
LpnPI CCDG 7 cut(s) 65, 150, 159, 279, 390, 420, 459
Lsp1109I GCAGC 2 cut(s) 61, 244
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 3 cut(s) 61, 139, 533
MalI GATC 2 cut(s) 150, 428
MboI GATC 2 cut(s) 148, 426
MboII GAAGA 5 cut(s) 143, 209, 212, 215, 310
MlsI TGGCCA 1 cut(s) 445
MluCI AATT 2 cut(s) 438, 551
MluNI TGGCCA 1 cut(s) 445
MnlI CCTC 2 cut(s) 41, 492
Mox20I TGGCCA 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 216
MscI TGGCCA 1 cut(s) 445
MseI TTAA 2 cut(s) 192, 387
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 36, 83, 92, 212, 451
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 1 cut(s) 506
NdeII GATC 2 cut(s) 148, 426
NlaIII CATG 5 cut(s) 62, 158, 212, 251, 360
NlaIV GGNNCC 1 cut(s) 135
PciSI GCTCTTC 2 cut(s) 12, 222
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 495
PkrI GCNGC 2 cut(s) 76, 259
PshBI ATTAAT 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 74
SapI GCTCTTC 2 cut(s) 12, 222
SaqAI TTAA 2 cut(s) 192, 387
SatI GCNGC 2 cut(s) 75, 258
Sau3AI GATC 2 cut(s) 148, 426
Sau96I GGNCC 1 cut(s) 479
SetI ASST 8 cut(s) 76, 148, 177, 217, 233, 295, 402, 461
SfaNI GCATC 1 cut(s) 73
SinI GGWCC 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 438, 551
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 10, 429
TasI AATT 2 cut(s) 438, 551
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 192, 387
Tru9I TTAA 2 cut(s) 192, 387
TscAI CASTG 1 cut(s) 439
TseI GCWGC 2 cut(s) 74, 257
TspDTI ATGAA 5 cut(s) 47, 143, 215, 225, 564
TspRI CASTG 1 cut(s) 439
Van91I CCANNNNNTGG 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 479
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 2 cut(s) 438, 551
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.