Rh1DG076500

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005206
Physical Location & Seq
Reverse (-)
14109755 .. 14117443
7689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG076500.1

Sequence Viewer

Length: 495 bp
ATGGCTCTTCGATGGCTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTACTTGTCGATGTTACTCACTCAACTGTTCAGGATGTCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTATCCATGTTACCGGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAACAGCAACATTGATATTGCACATGAAGCTCTTCTTCTTCACAGCTACAGGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATTGCTGCAAAGATAAACCAAAGCCAGTTTGAACAAGGTCTGTGTAATCGGTGTATCCGCACCTGCTTTTACATCAGAAACCATAATCAAACGAAAAAACATAAGAAATTTGAAATGAGTTTTTTGGGGTTTAAGCTAAGCCTACCGAGATTGGCTATGAGAGAGGAAGTGAAGCTCATTAGTACGAGAAAGATGGAGATGAGCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

164

Amino Acids

18.94

Weight (kDa)

9.39

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 98 2.3e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 356
AciI CCGC 1 cut(s) 343
AclWI GGATC 1 cut(s) 156
AcsI RAATTY 1 cut(s) 392
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 128
AgsI TTSAA 3 cut(s) 244, 317, 398
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 5 cut(s) 212, 228, 421, 460, 489
AluI AGCT 5 cut(s) 212, 228, 421, 460, 489
AlwI GGATC 1 cut(s) 156
ApeKI GCWGC 2 cut(s) 254, 290
ApoI RAATTY 1 cut(s) 392
Asp700I GAANNNNTTC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 241, 277
BccI CCATC 2 cut(s) 6, 472
BciVI GTATCC 2 cut(s) 145, 350
BfmI CTRYAG 2 cut(s) 167, 229
BfuAI ACCTGC 1 cut(s) 356
BfuI GTATCC 2 cut(s) 145, 350
BisI GCNGC 2 cut(s) 255, 291
BlpI GCTNAGC 1 cut(s) 422
BlsI GCNGC 2 cut(s) 256, 292
Bpu1102I GCTNAGC 1 cut(s) 422
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 264
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 2 cut(s) 241, 277
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 128
Bsp143I GATC 1 cut(s) 148
Bsp1720I GCTNAGC 1 cut(s) 422
BspACI CCGC 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 356
BspPI GGATC 1 cut(s) 156
BspQI GCTCTTC 2 cut(s) 12, 219
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 1 cut(s) 310
BssMI GATC 1 cut(s) 148
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 2 cut(s) 12, 219
BstDEI CTNAG 2 cut(s) 26, 422
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 36, 209, 268
BstSFI CTRYAG 2 cut(s) 167, 229
BstV1I GCAGC 2 cut(s) 241, 277
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
BsuI GTATCC 2 cut(s) 145, 350
BtsCI GGATG 1 cut(s) 88
BveI ACCTGC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 468
CviAII CATG 3 cut(s) 139, 155, 206
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 2 cut(s) 26, 422
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 12, 219
EarI CTCTTC 2 cut(s) 12, 219
FaeI CATG 3 cut(s) 142, 158, 209
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FatI CATG 3 cut(s) 138, 154, 205
Fnu4HI GCNGC 2 cut(s) 255, 291
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 2 cut(s) 255, 291
GluI GCNGC 2 cut(s) 255, 291
HapII CCGG 1 cut(s) 146
Hin1II CATG 3 cut(s) 142, 158, 209
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HpaII CCGG 1 cut(s) 146
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 89, 362
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 39, 203, 257, 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 209, 268
HpyF3I CTNAG 2 cut(s) 26, 422
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 3 cut(s) 142, 158, 209
Kzo9I GATC 1 cut(s) 148
LguI GCTCTTC 2 cut(s) 12, 219
LpnPI CCDG 6 cut(s) 65, 155, 159, 217, 323, 361
Lsp1109I GCAGC 2 cut(s) 241, 277
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 61, 141
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 4 cut(s) 143, 206, 209, 212
MflI RGATCY 1 cut(s) 148
MluCI AATT 2 cut(s) 285, 392
MnlI CCTC 2 cut(s) 41, 442
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 1 cut(s) 417
MspI CCGG 1 cut(s) 146
MwoI GCNNNNNNNGC 3 cut(s) 36, 209, 268
NdeII GATC 1 cut(s) 148
NlaIII CATG 3 cut(s) 142, 158, 209
PaqCI CACCTGC 1 cut(s) 356
PciSI GCTCTTC 2 cut(s) 12, 219
PdmI GAANNNNTTC 1 cut(s) 213
PkrI GCNGC 2 cut(s) 256, 292
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
SapI GCTCTTC 2 cut(s) 12, 219
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 2 cut(s) 255, 291
Sau3AI GATC 1 cut(s) 148
SetI ASST 8 cut(s) 177, 214, 230, 325, 350, 423, 462, 491
SfcI CTRYAG 2 cut(s) 167, 229
Sse9I AATT 2 cut(s) 285, 392
SsiI CCGC 1 cut(s) 343
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 10, 57
TasI AATT 2 cut(s) 285, 392
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseI GCWGC 2 cut(s) 254, 290
TspDTI ATGAA 2 cut(s) 143, 222
XapI RAATTY 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.