Rh1DG354300

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005206
Physical Location & Seq
Forward (+)
57782692 .. 57793938
11247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG354300.1

Sequence Viewer

Length: 516 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAATATTCTACTGCTTGTCGATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCGAAGAACGGGCCCATATTACCTGATCATCATGTGGATATGCTACAGGACGTAGTTGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGACAGTGGTGAGAAAGTGAAGGATACAAATAACTATATGATTACAAAAAACAAGGCTCAAACGTGTTTATGCCCAAAGCAGTGCAGCAGCCACATTGAAGCTCCGAGGATTTGTGATCATGATGTACAGATCAAACAAGATAATGCTGATTTCACAGTAGAGATTGGCTTCTGCGAAGGATATAGGATGGGCGGGCAAAATGATCAAGTCTCAGCTCAGCTCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

171

Amino Acids

19.26

Weight (kDa)

5.39

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 95 1.7e-09 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 480
AfaI GTAC 1 cut(s) 414
AfiI CCNNNNNNNGG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 350
AgsI TTSAA 2 cut(s) 241, 386
AluBI AGCT 5 cut(s) 74, 225, 389, 503, 508
AluI AGCT 5 cut(s) 74, 225, 389, 503, 508
Alw26I GTCTC 1 cut(s) 502
AoxI GGCC 1 cut(s) 131
ApaI GGGCCC 1 cut(s) 135
ApeKI GCWGC 4 cut(s) 74, 251, 372, 375
AspS9I GGNCC 2 cut(s) 131, 132
AsuHPI GGTGA 1 cut(s) 308
BaeGI GKGCMC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 135
BbvI GCAGC 4 cut(s) 61, 238, 384, 387
BccI CCATC 2 cut(s) 6, 469
BciVI GTATCC 1 cut(s) 304
BclI TGATCA 3 cut(s) 145, 403, 490
BcoDI GTCTC 1 cut(s) 502
BfaI CTAG 1 cut(s) 514
BfmI CTRYAG 1 cut(s) 164
BfuI GTATCC 1 cut(s) 304
BglI GCCNNNNNGGC 1 cut(s) 92
BglII AGATCT 1 cut(s) 208
BisI GCNGC 4 cut(s) 75, 252, 373, 376
BlpI GCTNAGC 1 cut(s) 504
BlsI GCNGC 4 cut(s) 76, 253, 374, 377
BmgT120I GGNCC 2 cut(s) 131, 132
BmiI GGNNCC 1 cut(s) 133
BmsI GCATC 1 cut(s) 73
Bpu10I CCTNAGC 1 cut(s) 78
Bpu1102I GCTNAGC 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 392
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse3DI GCAATG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 2 cut(s) 88, 480
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 3 cut(s) 84, 92, 513
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 4 cut(s) 61, 238, 384, 387
BsgI GTGCAG 1 cut(s) 391
BshFI GGCC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 502
BsnI GGCC 1 cut(s) 133
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 135
Bsp1407I TGTACA 1 cut(s) 412
Bsp143I GATC 5 cut(s) 145, 208, 403, 417, 490
Bsp1720I GCTNAGC 1 cut(s) 504
BspACI CCGC 1 cut(s) 480
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 3 cut(s) 83, 91, 512
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 261
BsrGI TGTACA 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 5 cut(s) 145, 208, 403, 417, 490
Bst4CI ACNGT 3 cut(s) 182, 293, 445
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 412
BstC8I GCNNGC 1 cut(s) 482
BstDEI CTNAG 4 cut(s) 70, 78, 499, 504
BstF5I GGATG 2 cut(s) 88, 480
BstKTI GATC 5 cut(s) 148, 211, 406, 420, 493
BstMAI GTCTC 1 cut(s) 502
BstMBI GATC 5 cut(s) 145, 208, 403, 417, 490
BstMWI GCNNNNNNNGC 3 cut(s) 83, 92, 265
BstSFI CTRYAG 1 cut(s) 164
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 4 cut(s) 61, 238, 384, 387
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 2 cut(s) 88, 480
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 2 cut(s) 298, 374
Cac8I GCNNGC 1 cut(s) 482
CciI TCATGA 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 131, 132
Csp6I GTAC 1 cut(s) 413
CviAII CATG 3 cut(s) 152, 203, 407
CviQI GTAC 1 cut(s) 413
DdeI CTNAG 4 cut(s) 70, 78, 499, 504
DpnI GATC 5 cut(s) 147, 210, 405, 419, 492
DpnII GATC 5 cut(s) 145, 208, 403, 417, 490
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 3 cut(s) 155, 206, 410
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FatI CATG 3 cut(s) 151, 202, 406
FauI CCCGC 1 cut(s) 473
FbaI TGATCA 3 cut(s) 145, 403, 490
Fnu4HI GCNGC 4 cut(s) 75, 252, 373, 376
FokI GGATG 2 cut(s) 95, 487
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 75, 252, 373, 376
FspBI CTAG 1 cut(s) 514
GluI GCNGC 4 cut(s) 75, 252, 373, 376
HaeIII GGCC 1 cut(s) 133
Hin1II CATG 3 cut(s) 155, 206, 410
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 2 cut(s) 80, 407
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 3 cut(s) 32, 301, 458
HpyCH4III ACNGT 3 cut(s) 182, 293, 445
HpyCH4IV ACGT 2 cut(s) 171, 350
HpyCH4V TGCA 4 cut(s) 39, 200, 254, 372
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 92, 265
HpyF3I CTNAG 4 cut(s) 70, 78, 499, 504
HpySE526I ACGT 2 cut(s) 171, 350
Hsp92II CATG 3 cut(s) 155, 206, 410
Ksp22I TGATCA 3 cut(s) 145, 403, 490
Kzo9I GATC 5 cut(s) 145, 208, 403, 417, 490
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 3 cut(s) 65, 152, 156
Lsp1109I GCAGC 4 cut(s) 61, 238, 384, 387
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 514
MaeII ACGT 2 cut(s) 171, 350
MaeIII GTNAC 1 cut(s) 61
MalI GATC 5 cut(s) 147, 210, 405, 419, 492
MboI GATC 5 cut(s) 145, 208, 403, 417, 490
MboII GAAGA 3 cut(s) 136, 209, 218
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 135
MnlI CCTC 2 cut(s) 41, 387
MspA1I CMGCKG 1 cut(s) 74
MwoI GCNNNNNNNGC 3 cut(s) 83, 92, 265
NdeII GATC 5 cut(s) 145, 208, 403, 417, 490
NlaIII CATG 3 cut(s) 155, 206, 410
NlaIV GGNNCC 1 cut(s) 133
PagI TCATGA 1 cut(s) 406
PciSI GCTCTTC 1 cut(s) 12
PkrI GCNGC 4 cut(s) 76, 253, 374, 377
PspN4I GGNNCC 1 cut(s) 133
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 2 cut(s) 131, 132
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 4 cut(s) 75, 252, 373, 376
Sau3AI GATC 5 cut(s) 145, 208, 403, 417, 490
Sau96I GGNCC 2 cut(s) 131, 132
SduI GDGCHC 1 cut(s) 135
SetI ASST 9 cut(s) 33, 76, 145, 174, 227, 353, 391, 505, 510
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 164
SsiI CCGC 1 cut(s) 480
SspI AATATT 1 cut(s) 43
SspMI CTAG 1 cut(s) 514
TaaI ACNGT 3 cut(s) 182, 293, 445
TaiI ACGT 2 cut(s) 174, 353
TaqI TCGA 2 cut(s) 10, 57
TatI WGTACW 1 cut(s) 412
TscAI CASTG 2 cut(s) 298, 374
TseI GCWGC 4 cut(s) 74, 251, 372, 375
TspDTI ATGAA 1 cut(s) 219
TspRI CASTG 2 cut(s) 298, 374
XspI CTAG 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.