Rh1AG072500

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005206
Physical Location & Seq
Reverse (-)
12145820 .. 12148007
2188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG072500.1

Sequence Viewer

Length: 486 bp
ATGGTCAACTATCTCAAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGAGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTAATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGGAATGGATGGTGTAGTGTCTATCTTCCCAAGCAAACAAAGGAGCGTGAAAACAACAAAGTCATGGAACTTCCTTGGTTTTCCAGAAACAGTTAAGAGAAGCGCCCTTGAAAGGGATATCATCATCGGTGTGATGGACAGTGGAATTTGGCCAGAGTCAGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAAGTATGCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

161

Amino Acids

17.99

Weight (kDa)

8.82

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 12 - 91 1.1e-14 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 377
AcsI RAATTY 1 cut(s) 372
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 339, 340
AgsI TTSAA 2 cut(s) 181, 338
AluBI AGCT 3 cut(s) 149, 165, 393
AluI AGCT 3 cut(s) 149, 165, 393
AoxI GGCC 1 cut(s) 377
ApeKI GCWGC 2 cut(s) 123, 191
ApoI RAATTY 1 cut(s) 372
AseI ATTAAT 1 cut(s) 132
Asp718I GGTACC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 413
AvaII GGWCC 1 cut(s) 413
BalI TGGCCA 1 cut(s) 379
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 135, 178
BccI CCATC 2 cut(s) 230, 355
BciVI GTATCC 1 cut(s) 13
BfmI CTRYAG 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 333
BfuI GTATCC 1 cut(s) 13
BisI GCNGC 2 cut(s) 124, 192
BlsI GCNGC 2 cut(s) 125, 193
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 1 cut(s) 72
BmsI GCATC 1 cut(s) 391
BsaJI CCNNGG 1 cut(s) 301
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 340
Bse3DI GCAATG 2 cut(s) 201, 457
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 2 cut(s) 339, 340
BseMI GCAATG 2 cut(s) 201, 457
BseXI GCAGC 2 cut(s) 135, 178
BshFI GGCC 1 cut(s) 379
BshNI GGYRCC 1 cut(s) 70
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 339, 340
BsnI GGCC 1 cut(s) 379
Bsp143I GATC 1 cut(s) 85
BspANI GGCC 1 cut(s) 379
BspLI GGNNCC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 201, 457
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 319, 368
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 1 cut(s) 88
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 146, 205
BstSFI CTRYAG 1 cut(s) 104
BstV1I GCAGC 2 cut(s) 135, 178
BsuI GTATCC 1 cut(s) 13
BsuRI GGCC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 241
BtsIMutI CAGTG 1 cut(s) 373
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 1 cut(s) 71
CviAII CATG 3 cut(s) 92, 143, 291
CviJI RGCY 6 cut(s) 32, 58, 149, 165, 379, 393
CviKI_1 RGCY 6 cut(s) 32, 58, 149, 165, 379, 393
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 1 cut(s) 423
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 301
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 413
EcoRV GATATC 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 3 cut(s) 95, 146, 294
FaiI YATR 9 cut(s) 36, 45, 47, 77, 93, 101, 144, 292, 477
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 3 cut(s) 91, 142, 290
Fnu4HI GCNGC 2 cut(s) 124, 192
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 2 cut(s) 124, 192
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 124, 192
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 1 cut(s) 379
HapII CCGG 1 cut(s) 228
HhaI GCGC 1 cut(s) 332
Hin1II CATG 3 cut(s) 95, 146, 294
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HincII GTYRAC 2 cut(s) 7, 118
HindII GTYRAC 2 cut(s) 7, 118
HinfI GANTC 1 cut(s) 383
HpaII CCGG 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 7, 118
Hpy188I TCNGA 1 cut(s) 388
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 7, 118
HpyCH4III ACNGT 2 cut(s) 319, 368
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 2 cut(s) 140, 194
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 205
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 3 cut(s) 95, 146, 294
HspAI GCGC 1 cut(s) 330
KpnI GGTACC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 1 cut(s) 270
LpnPI CCDG 6 cut(s) 92, 96, 241, 324, 390, 393
Lsp1109I GCAGC 2 cut(s) 135, 178
LweI GCATC 1 cut(s) 391
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 467
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MboII GAAGA 3 cut(s) 80, 149, 244
MlsI TGGCCA 1 cut(s) 379
MluCI AATT 1 cut(s) 372
MluNI TGGCCA 1 cut(s) 379
MlyI GAGTC 1 cut(s) 392
MnlI CCTC 2 cut(s) 61, 426
Mox20I TGGCCA 1 cut(s) 379
MscI TGGCCA 1 cut(s) 379
MseI TTAA 2 cut(s) 132, 321
MslI CAYNNNNRTG 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 379
MspI CCGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 146, 205
NdeII GATC 1 cut(s) 85
NlaIII CATG 3 cut(s) 95, 146, 294
NlaIV GGNNCC 1 cut(s) 72
PkrI GCNGC 2 cut(s) 125, 193
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PshBI ATTAAT 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 413
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 2 cut(s) 132, 321
SatI GCNGC 2 cut(s) 124, 192
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 1 cut(s) 392
SetI ASST 7 cut(s) 72, 85, 114, 151, 167, 395, 424
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 1 cut(s) 372
SspI AATATT 1 cut(s) 136
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 2 cut(s) 319, 368
TaiI ACGT 1 cut(s) 114
TasI AATT 1 cut(s) 372
Tru1I TTAA 2 cut(s) 132, 321
Tru9I TTAA 2 cut(s) 132, 321
TscAI CASTG 1 cut(s) 373
TseI GCWGC 2 cut(s) 123, 191
TspDTI ATGAA 2 cut(s) 80, 159
TspRI CASTG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 413
VspI ATTAAT 1 cut(s) 132
XapI RAATTY 1 cut(s) 372
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.