Rh1DG347100

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005206
Physical Location & Seq
Forward (+)
57077467 .. 57077942
476 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG347100.1

Sequence Viewer

Length: 324 bp
ATGCCTCTTCGATGGCTACTCCTTCTCAGCCTCATTGCCAATATTCTACTTCTCATTGATGCTACTCAATCAGTTGCTCGGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTACGAGATGTAGTTGACAACAACATTAATAATGATATTTCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTAGCTGGTACTCCTGCAATCCTACATTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

107

Amino Acids

11.93

Weight (kDa)

6.96

Isoelectric Point (pI)

59.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 103 4.4e-12 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 133
AfaI GTAC 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 128
AluBI AGCT 3 cut(s) 215, 231, 293
AluI AGCT 3 cut(s) 215, 231, 293
Ama87I CYCGRG 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 257
AseI ATTAAT 1 cut(s) 192
AvaI CYCGRG 1 cut(s) 78
BanI GGYRCC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 6
BfaI CTAG 1 cut(s) 290
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 78
BmiI GGNNCC 1 cut(s) 135
BmsI GCATC 2 cut(s) 49, 73
BsaJI CCNNGG 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 137
Bse3DI GCAATG 2 cut(s) 33, 267
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 2 cut(s) 33, 267
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 78
Bsp143I GATC 1 cut(s) 148
Bsp19I CCATGG 1 cut(s) 247
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 2 cut(s) 33, 267
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 26
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 83, 92
BstV1I GCAGC 1 cut(s) 244
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 88
Csp6I GTAC 1 cut(s) 297
CviAII CATG 3 cut(s) 155, 209, 248
CviJI RGCY 7 cut(s) 16, 30, 95, 121, 215, 231, 293
CviKI_1 RGCY 7 cut(s) 16, 30, 95, 121, 215, 231, 293
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 1 cut(s) 26
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 247
Eco88I CYCGRG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 247
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 3 cut(s) 158, 212, 251
FaiI YATR 6 cut(s) 99, 108, 110, 156, 210, 249
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 3 cut(s) 154, 208, 247
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 1 cut(s) 290
GluI GCNGC 1 cut(s) 258
Hin1II CATG 3 cut(s) 158, 212, 251
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 181
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 32
HpyCH4V TGCA 2 cut(s) 260, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 92
HpyF3I CTNAG 1 cut(s) 26
Hsp92II CATG 3 cut(s) 158, 212, 251
Kzo9I GATC 1 cut(s) 148
LpnPI CCDG 4 cut(s) 150, 159, 279, 315
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 2 cut(s) 49, 73
MaeI CTAG 1 cut(s) 290
MaeIII GTNAC 1 cut(s) 139
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 2 cut(s) 143, 215
MnlI CCTC 2 cut(s) 15, 41
MseI TTAA 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 83, 92
NcoI CCATGG 1 cut(s) 247
NdeII GATC 1 cut(s) 148
NlaIII CATG 3 cut(s) 158, 212, 251
NlaIV GGNNCC 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PkrI GCNGC 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 135
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 1 cut(s) 148
SetI ASST 4 cut(s) 148, 217, 233, 295
SfaNI GCATC 2 cut(s) 49, 73
SspI AATATT 2 cut(s) 43, 163
SspMI CTAG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 247
TaqI TCGA 1 cut(s) 10
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 2 cut(s) 143, 225
VspI ATTAAT 1 cut(s) 192
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.