Rh1DG349600

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005206
Physical Location & Seq
Reverse (-)
57472161 .. 57472631
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG349600.1

Sequence Viewer

Length: 324 bp
ATGGCTCTTCGTTGGTTTCTCTTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTTGTCGATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAAGACGTAGTTGACAATAGCAACATTGATATTGCACATGAATATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGTACTGCTGCAACCCTACCTTTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

107

Amino Acids

12.0

Weight (kDa)

6.15

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 97 2.9e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 136
AfaI GTAC 2 cut(s) 138, 298
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 1 cut(s) 247
AluBI AGCT 2 cut(s) 77, 231
AluI AGCT 2 cut(s) 77, 231
ApeKI GCWGC 3 cut(s) 77, 257, 302
Asp718I GGTACC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 136
BbvI GCAGC 3 cut(s) 64, 244, 289
BglI GCCNNNNNGGC 1 cut(s) 95
BisI GCNGC 3 cut(s) 78, 258, 303
BlsI GCNGC 3 cut(s) 79, 259, 304
BmiI GGNNCC 1 cut(s) 138
BmsI GCATC 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 267
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 3 cut(s) 40, 87, 95
BseXI GCAGC 3 cut(s) 64, 244, 289
BshNI GGYRCC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 131
Bsp143I GATC 1 cut(s) 151
BspCNI CTCAG 3 cut(s) 39, 86, 94
BspLI GGNNCC 1 cut(s) 138
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 267
BssMI GATC 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 12
BstDEI CTNAG 3 cut(s) 26, 73, 81
BstF5I GGATG 1 cut(s) 91
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 4 cut(s) 36, 86, 95, 271
BstV1I GCAGC 3 cut(s) 64, 244, 289
BtsCI GGATG 1 cut(s) 91
Csp6I GTAC 2 cut(s) 137, 297
CviAII CATG 2 cut(s) 158, 209
CviJI RGCY 6 cut(s) 5, 30, 77, 98, 124, 231
CviKI_1 RGCY 6 cut(s) 5, 30, 77, 98, 124, 231
CviQI GTAC 2 cut(s) 137, 297
DdeI CTNAG 3 cut(s) 26, 73, 81
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
FaeI CATG 2 cut(s) 161, 212
FaiI YATR 7 cut(s) 102, 111, 113, 143, 159, 167, 210
FatI CATG 2 cut(s) 157, 208
Fnu4HI GCNGC 3 cut(s) 78, 258, 303
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 3 cut(s) 78, 258, 303
GluI GCNGC 3 cut(s) 78, 258, 303
Hin1II CATG 2 cut(s) 161, 212
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
Hpy166II GTNNAC 1 cut(s) 184
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 39, 206, 260, 305, 319
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 86, 95, 271
HpyF3I CTNAG 3 cut(s) 26, 73, 81
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 2 cut(s) 161, 212
KpnI GGTACC 1 cut(s) 140
Kzo9I GATC 1 cut(s) 151
LguI GCTCTTC 1 cut(s) 12
LpnPI CCDG 2 cut(s) 68, 162
Lsp1109I GCAGC 3 cut(s) 64, 244, 289
LweI GCATC 1 cut(s) 76
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 64
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 2 cut(s) 146, 215
MnlI CCTC 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 77
MwoI GCNNNNNNNGC 4 cut(s) 36, 86, 95, 271
NdeII GATC 1 cut(s) 151
NlaIII CATG 2 cut(s) 161, 212
NlaIV GGNNCC 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 12
PkrI GCNGC 3 cut(s) 79, 259, 304
PspN4I GGNNCC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 2 cut(s) 138, 298
RsaNI GTAC 2 cut(s) 137, 297
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 3 cut(s) 78, 258, 303
Sau3AI GATC 1 cut(s) 151
SetI ASST 5 cut(s) 79, 151, 180, 233, 316
SfaNI GCATC 1 cut(s) 76
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 60
TseI GCWGC 3 cut(s) 77, 257, 302
TspDTI ATGAA 2 cut(s) 146, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.