Rh4CG073800

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005206
Physical Location & Seq
Forward (+)
13619388 .. 13628525
9138 bp
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UTR
Exon/CDS
Intron
Rh4CG073800.1

Sequence Viewer

Length: 648 bp
ATGGCTCTTCGATGGTTTCTCCTTCTTAGCCTCATTGGAAGTATTCTACTTGTTGTTGTTACTCAGTCAGCTGCTCAGGATTCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAATGGGGTGCAAGTAACACCTGATTTTCATGTGAATATTCTACGAGACGTAGTTGACAGCAGCATTAATAATGATATTGCACATGAATCTCTGCTTCTTCATAGCTACAAGAGAAGTTTCCATGGTTTTGCTGCAATGCTCACTGAACAAGAAGCACAAAAACTAGCTGGAATGGAGGGTGTAGTGTCTGTCTTCCCAAGCAAAAAGAGGAGCATCAAAACAACAAAACTAGGCCGCCAAAGAAAAAAAATTCTCTCTCGACAGAAAATCCCAGCAACTCTTCGAATTCGATCATCTCTTCCCCAGAGGACTGAAATGGGAGAGGAGGAAGGGAGAATTTATCAAGGCCAAGAAAGGTGGTTGTTTGTGAATTTTGCAGAATGGAATAGCAGAGAAGAATTGGCGGGGGAGAGTTTGGGTTCGGTTTTCGTTGGGATCGACAATGAGAATTGGGAACCTACTAAAAGCTCTTATCTAGGATTGCATGGAGCAAAACTCGATAACAAAGGGTTCCATCATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

215

Amino Acids

24.34

Weight (kDa)

9.39

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 113 6.1e-16 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 125
AciI CCGC 2 cut(s) 358, 527
AclWI GGATC 1 cut(s) 566
AcsI RAATTY 4 cut(s) 372, 408, 459, 493
AfiI CCNNNNNNNGG 1 cut(s) 125
AluBI AGCT 4 cut(s) 71, 228, 290, 591
AluI AGCT 4 cut(s) 71, 228, 290, 591
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 1 cut(s) 566
AoxI GGCC 2 cut(s) 355, 469
ApeKI GCWGC 3 cut(s) 71, 183, 254
ApoI RAATTY 4 cut(s) 372, 408, 459, 493
AseI ATTAAT 1 cut(s) 189
AsuII TTCGAA 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 3 cut(s) 58, 195, 241
BccI CCATC 2 cut(s) 6, 645
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 3 cut(s) 287, 353, 599
BisI GCNGC 4 cut(s) 72, 184, 255, 358
BlsI GCNGC 4 cut(s) 73, 185, 256, 359
BmiI GGNNCC 2 cut(s) 579, 635
BmsI GCATC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 603, 635
Bpu10I CCTNAGC 1 cut(s) 75
Bpu14I TTCGAA 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 77, 89
BseRI GAGGAG 2 cut(s) 346, 461
BseXI GCAGC 3 cut(s) 58, 195, 241
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 2 cut(s) 357, 471
BslI CCNNNNNNNGG 1 cut(s) 125
BsmAI GTCTC 1 cut(s) 162
BsmBI CGTCTC 1 cut(s) 162
BsnI GGCC 2 cut(s) 357, 471
Bsp119I TTCGAA 1 cut(s) 406
Bsp143I GATC 2 cut(s) 413, 558
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 2 cut(s) 358, 527
BspANI GGCC 2 cut(s) 357, 471
BspCNI CTCAG 2 cut(s) 76, 88
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 2 cut(s) 579, 635
BspPI GGATC 1 cut(s) 566
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 2 cut(s) 413, 558
BssT1I CCWWGG 1 cut(s) 244
Bst6I CTCTTC 3 cut(s) 12, 408, 426
BstBI TTCGAA 1 cut(s) 406
BstDEI CTNAG 3 cut(s) 26, 63, 75
BstDSI CCRYGG 1 cut(s) 244
BstKTI GATC 2 cut(s) 416, 561
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 2 cut(s) 413, 558
BstV1I GCAGC 3 cut(s) 58, 195, 241
BstV2I GAAGAC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 357, 471
BtgI CCRYGG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 264
CciI TCATGA 1 cut(s) 640
CspCI CAANNNNNGTGG 2 cut(s) 461, 496
CviAII CATG 5 cut(s) 152, 206, 245, 608, 641
DdeI CTNAG 3 cut(s) 26, 63, 75
DpnI GATC 2 cut(s) 415, 560
DpnII GATC 2 cut(s) 413, 558
Eam1104I CTCTTC 3 cut(s) 12, 408, 426
EarI CTCTTC 3 cut(s) 12, 408, 426
Eco130I CCWWGG 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 5 cut(s) 155, 209, 248, 611, 644
FaiI YATR 9 cut(s) 96, 105, 107, 153, 207, 225, 246, 609, 642
FatI CATG 5 cut(s) 151, 205, 244, 607, 640
FauI CCCGC 1 cut(s) 520
Fnu4HI GCNGC 4 cut(s) 72, 184, 255, 358
Fsp4HI GCNGC 4 cut(s) 72, 184, 255, 358
FspBI CTAG 3 cut(s) 287, 353, 599
GluI GCNGC 4 cut(s) 72, 184, 255, 358
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 2 cut(s) 357, 471
Hin1II CATG 5 cut(s) 155, 209, 248, 611, 644
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 80, 209
Hpy166II GTNNAC 1 cut(s) 178
Hpy188III TCNNGA 4 cut(s) 77, 84, 381, 641
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 32, 446
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 133, 203, 257, 500, 607
HpyF3I CTNAG 3 cut(s) 26, 63, 75
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 5 cut(s) 155, 209, 248, 611, 644
Kzo9I GATC 2 cut(s) 413, 558
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 333, 611
LpnPI CCDG 5 cut(s) 62, 156, 276, 408, 440
Lsp1109I GCAGC 3 cut(s) 58, 195, 241
LweI GCATC 1 cut(s) 345
MaeI CTAG 3 cut(s) 287, 353, 599
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 58, 136
MalI GATC 2 cut(s) 415, 560
MboI GATC 2 cut(s) 413, 558
MboII GAAGA 5 cut(s) 212, 307, 395, 413, 530
MluCI AATT 6 cut(s) 372, 408, 459, 493, 521, 571
MnlI CCTC 6 cut(s) 41, 292, 324, 423, 439, 442
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 244
NdeII GATC 2 cut(s) 413, 558
NlaIII CATG 5 cut(s) 155, 209, 248, 611, 644
NlaIV GGNNCC 2 cut(s) 579, 635
NspV TTCGAA 1 cut(s) 406
PagI TCATGA 1 cut(s) 640
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PfeI GAWTC 2 cut(s) 80, 209
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 4 cut(s) 73, 185, 256, 359
PshBI ATTAAT 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 579, 635
PvuII CAGCTG 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 72, 184, 255, 358
Sau3AI GATC 2 cut(s) 413, 558
SetI ASST 8 cut(s) 73, 145, 174, 230, 292, 482, 583, 593
SfaNI GCATC 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 406
Sse9I AATT 6 cut(s) 372, 408, 459, 493, 521, 571
SsiI CCGC 2 cut(s) 358, 527
SspI AATATT 1 cut(s) 160
SspMI CTAG 3 cut(s) 287, 353, 599
StyI CCWWGG 1 cut(s) 244
TaiI ACGT 1 cut(s) 174
TaqI TCGA 6 cut(s) 10, 382, 406, 412, 561, 621
TasI AATT 6 cut(s) 372, 408, 459, 493, 521, 571
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 2 cut(s) 80, 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 271
TseI GCWGC 3 cut(s) 71, 183, 254
TspDTI ATGAA 3 cut(s) 140, 212, 222
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 125
VspI ATTAAT 1 cut(s) 189
XapI RAATTY 4 cut(s) 372, 408, 459, 493
XspI CTAG 3 cut(s) 287, 353, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.