Rh4DG063600

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005206
Physical Location & Seq
Forward (+)
9529310 .. 9529778
469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG063600.1

Sequence Viewer

Length: 321 bp
ATGGCTCTTCGATGGTTTCTCCTTCTTAGCCTCATTGGAAGTATTCTACTTGTTGATGTTACTCAGTCAGCTGCTCAGGATGCCCGAAAGACTTATATTGTGTATATGGGTGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATTTTCATGTGAATATTCTAAGAGACGTAGTTGACAGCAGCATTAATAATGATATTGCACATGAATCTCTGCTTCTTCATAGCTACAAGAGAAGTTTCCATGGTTTTGCTGCAATGCTCACTGAACAAGAAGCACAAAAACTGGCTGGTATTCCTGCAACCCTACCTTTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

106

Amino Acids

11.73

Weight (kDa)

6.39

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 98 2.7e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 125
AluBI AGCT 2 cut(s) 71, 228
AluI AGCT 2 cut(s) 71, 228
Alw26I GTCTC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 71, 183, 254
AseI ATTAAT 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 122
BanI GGYRCC 1 cut(s) 130
BbvI GCAGC 3 cut(s) 58, 195, 241
BccI CCATC 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 162
BisI GCNGC 3 cut(s) 72, 184, 255
BlsI GCNGC 3 cut(s) 73, 185, 256
BmiI GGNNCC 1 cut(s) 132
BmsI GCATC 1 cut(s) 70
Bpu10I CCTNAGC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 134, 291
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 77, 89
BseNI ACTGG 2 cut(s) 134, 291
BseXI GCAGC 3 cut(s) 58, 195, 241
BshNI GGYRCC 1 cut(s) 130
BslI CCNNNNNNNGG 1 cut(s) 125
BsmAI GTCTC 1 cut(s) 162
BsmBI CGTCTC 1 cut(s) 162
Bsp19I CCATGG 1 cut(s) 244
BspCNI CTCAG 2 cut(s) 76, 88
BspLI GGNNCC 1 cut(s) 132
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 264
BsrI ACTGG 2 cut(s) 134, 291
BssECI CCNNGG 1 cut(s) 244
BssT1I CCWWGG 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 12
BstDEI CTNAG 4 cut(s) 26, 63, 75, 164
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 85
BstMAI GTCTC 1 cut(s) 162
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 58, 195, 241
BtgI CCRYGG 1 cut(s) 244
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 264
CviAII CATG 3 cut(s) 152, 206, 245
CviJI RGCY 6 cut(s) 5, 30, 71, 118, 228, 290
CviKI_1 RGCY 6 cut(s) 5, 30, 71, 118, 228, 290
DdeI CTNAG 4 cut(s) 26, 63, 75, 164
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 3 cut(s) 155, 209, 248
FaiI YATR 7 cut(s) 96, 105, 107, 153, 207, 225, 246
FatI CATG 3 cut(s) 151, 205, 244
Fnu4HI GCNGC 3 cut(s) 72, 184, 255
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 3 cut(s) 72, 184, 255
GluI GCNGC 3 cut(s) 72, 184, 255
Hin1II CATG 3 cut(s) 155, 209, 248
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 1 cut(s) 209
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 178
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 1 cut(s) 32
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 203, 257, 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 4 cut(s) 26, 63, 75, 164
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 3 cut(s) 155, 209, 248
LguI GCTCTTC 1 cut(s) 12
LpnPI CCDG 6 cut(s) 62, 147, 156, 272, 276, 312
Lsp1109I GCAGC 3 cut(s) 58, 195, 241
LweI GCATC 1 cut(s) 70
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 58, 110, 136
MboII GAAGA 1 cut(s) 212
MnlI CCTC 1 cut(s) 41
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 80
NcoI CCATGG 1 cut(s) 244
NlaIII CATG 3 cut(s) 155, 209, 248
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 110
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 3 cut(s) 73, 185, 256
PshBI ATTAAT 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 3 cut(s) 72, 184, 255
SetI ASST 5 cut(s) 73, 145, 174, 230, 313
SfaNI GCATC 1 cut(s) 70
SspI AATATT 1 cut(s) 160
StyI CCWWGG 1 cut(s) 244
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 10
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 3 cut(s) 71, 183, 254
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 3 cut(s) 140, 212, 222
TspRI CASTG 1 cut(s) 271
VspI ATTAAT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.