Rh4BG066600

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005206
Physical Location & Seq
Forward (+)
11964215 .. 11970970
6756 bp
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UTR
Exon/CDS
Intron
Rh4BG066600.1

Sequence Viewer

Length: 384 bp
ATGGCTCTTCGATGGTTTCTCCTTCTTAGCCTCATTGGAAGTATTCTACTTGTTGTTGTTACTCAGTCAGCTGCTCAGGATTCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAATGGGGTGCAAGTAACACCTGATTTTCATGTGAATATTCTACGAGACGTAGTTGACAGCAGCATTAATAATGATATTGCACATGAATCTCTGCTTCTTCATAGCTACAAGAGAAGTTTCCATGGTTTTGCTGCAATGCTCACTGAACAAGAAGCACAAAAACTAGCTGGAATGGAGGGTGTAGTGTCTGTCTTCCCAAGCAAAAAGAGGAGCATCAAAACAACAAAGTCACTATGTTCGCTTGGCAATCGAATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

127

Amino Acids

14.08

Weight (kDa)

9.43

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 113 1.6e-16 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 125
AluBI AGCT 3 cut(s) 71, 228, 290
AluI AGCT 3 cut(s) 71, 228, 290
Alw26I GTCTC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 71, 183, 254
AseI ATTAAT 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 307
BbvI GCAGC 3 cut(s) 58, 195, 241
BccI CCATC 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 1 cut(s) 287
BisI GCNGC 3 cut(s) 72, 184, 255
BlsI GCNGC 3 cut(s) 73, 185, 256
BmsI GCATC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 125
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 77, 89
BseRI GAGGAG 1 cut(s) 346
BseXI GCAGC 3 cut(s) 58, 195, 241
BslI CCNNNNNNNGG 1 cut(s) 125
BsmAI GTCTC 1 cut(s) 162
BsmBI CGTCTC 1 cut(s) 162
Bsp19I CCATGG 1 cut(s) 244
BspCNI CTCAG 2 cut(s) 76, 88
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 244
BssT1I CCWWGG 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 12
BstDEI CTNAG 3 cut(s) 26, 63, 75
BstDSI CCRYGG 1 cut(s) 244
BstMAI GTCTC 1 cut(s) 162
BstV1I GCAGC 3 cut(s) 58, 195, 241
BstV2I GAAGAC 1 cut(s) 307
BtgI CCRYGG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 264
CviAII CATG 3 cut(s) 152, 206, 245
CviJI RGCY 7 cut(s) 5, 30, 71, 92, 118, 228, 290
CviKI_1 RGCY 7 cut(s) 5, 30, 71, 92, 118, 228, 290
DdeI CTNAG 3 cut(s) 26, 63, 75
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 162
FaeI CATG 3 cut(s) 155, 209, 248
FaiI YATR 8 cut(s) 96, 105, 107, 153, 207, 225, 246, 358
FatI CATG 3 cut(s) 151, 205, 244
Fnu4HI GCNGC 3 cut(s) 72, 184, 255
Fsp4HI GCNGC 3 cut(s) 72, 184, 255
FspBI CTAG 1 cut(s) 287
GluI GCNGC 3 cut(s) 72, 184, 255
Hin1II CATG 3 cut(s) 155, 209, 248
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 80, 209
Hpy166II GTNNAC 1 cut(s) 178
Hpy188III TCNNGA 2 cut(s) 77, 84
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 1 cut(s) 32
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 133, 203, 257
HpyF3I CTNAG 3 cut(s) 26, 63, 75
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 3 cut(s) 155, 209, 248
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 333
LpnPI CCDG 3 cut(s) 62, 156, 276
Lsp1109I GCAGC 3 cut(s) 58, 195, 241
LweI GCATC 1 cut(s) 345
MaeI CTAG 1 cut(s) 287
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 58, 136, 351
MboII GAAGA 2 cut(s) 212, 307
MnlI CCTC 3 cut(s) 41, 292, 324
MseI TTAA 2 cut(s) 189, 382
MspA1I CMGCKG 1 cut(s) 71
NcoI CCATGG 1 cut(s) 244
NlaIII CATG 3 cut(s) 155, 209, 248
NmuCI GTSAC 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 80, 209
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 3 cut(s) 73, 185, 256
PshBI ATTAAT 1 cut(s) 189
PvuII CAGCTG 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 189, 382
SatI GCNGC 3 cut(s) 72, 184, 255
SetI ASST 5 cut(s) 73, 145, 174, 230, 292
SfaNI GCATC 1 cut(s) 345
SspI AATATT 1 cut(s) 160
SspMI CTAG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 244
TaiI ACGT 1 cut(s) 174
TaqI TCGA 2 cut(s) 10, 373
TfiI GAWTC 2 cut(s) 80, 209
Tru1I TTAA 2 cut(s) 189, 382
Tru9I TTAA 2 cut(s) 189, 382
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 1 cut(s) 351
TseI GCWGC 3 cut(s) 71, 183, 254
Tsp45I GTSAC 1 cut(s) 351
TspDTI ATGAA 3 cut(s) 140, 212, 222
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 125
VspI ATTAAT 1 cut(s) 189
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.