Rh1CG339700

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0005206
Physical Location & Seq
Reverse (-)
61362554 .. 61363060
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG339700.1

Sequence Viewer

Length: 357 bp
ATGCCTCTAGCTAGTAGCACCATACAGAGTAATAGTATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAGTATTCTACTGCTTGTCGATGTTACTCATTCAGCTGCTCAGGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGGCAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATATGAATATGCTACAAGACGTAGTTGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGTACTGCTGCAACCCTACCTTTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

118

Amino Acids

12.99

Weight (kDa)

6.15

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 108 2.2e-09 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 169
AciI CCGC 1 cut(s) 150
AfaI GTAC 2 cut(s) 171, 331
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 1 cut(s) 280
AluBI AGCT 3 cut(s) 11, 110, 264
AluI AGCT 3 cut(s) 11, 110, 264
ApeKI GCWGC 3 cut(s) 110, 290, 335
Asp718I GGTACC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 169
BbvI GCAGC 3 cut(s) 97, 277, 322
BccI CCATC 1 cut(s) 42
BfaI CTAG 2 cut(s) 8, 12
BglI GCCNNNNNGGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 247
BisI GCNGC 4 cut(s) 111, 151, 291, 336
BlsI GCNGC 4 cut(s) 112, 152, 292, 337
BmiI GGNNCC 1 cut(s) 171
BmsI GCATC 1 cut(s) 109
Bpu10I CCTNAGC 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 300
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 97, 277, 322
BshNI GGYRCC 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 164
Bsp143I GATC 2 cut(s) 184, 247
BspACI CCGC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 171
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 300
BssMI GATC 2 cut(s) 184, 247
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 2 cut(s) 187, 250
BstMBI GATC 2 cut(s) 184, 247
BstMWI GCNNNNNNNGC 3 cut(s) 119, 128, 304
BstV1I GCAGC 3 cut(s) 97, 277, 322
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BtsCI GGATG 1 cut(s) 124
Cac8I GCNNGC 1 cut(s) 155
Csp6I GTAC 2 cut(s) 170, 330
CviAII CATG 1 cut(s) 242
CviJI RGCY 6 cut(s) 11, 41, 110, 131, 157, 264
CviKI_1 RGCY 6 cut(s) 11, 41, 110, 131, 157, 264
CviQI GTAC 2 cut(s) 170, 330
DdeI CTNAG 1 cut(s) 114
DpnI GATC 2 cut(s) 186, 249
DpnII GATC 2 cut(s) 184, 247
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
FaeI CATG 1 cut(s) 245
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 1 cut(s) 241
FauNDI CATATG 1 cut(s) 192
Fnu4HI GCNGC 4 cut(s) 111, 151, 291, 336
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 111, 151, 291, 336
FspBI CTAG 2 cut(s) 8, 12
GluI GCNGC 4 cut(s) 111, 151, 291, 336
Hin1II CATG 1 cut(s) 245
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 217
Hpy188III TCNNGA 1 cut(s) 116
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 5 cut(s) 75, 239, 293, 338, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 128, 304
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 1 cut(s) 245
KpnI GGTACC 1 cut(s) 173
Kzo9I GATC 2 cut(s) 184, 247
LguI GCTCTTC 1 cut(s) 48
LpnPI CCDG 2 cut(s) 101, 195
Lsp1109I GCAGC 3 cut(s) 97, 277, 322
LweI GCATC 1 cut(s) 109
MaeI CTAG 2 cut(s) 8, 12
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 186, 249
MboI GATC 2 cut(s) 184, 247
MboII GAAGA 4 cut(s) 35, 179, 248, 257
MflI RGATCY 1 cut(s) 247
MnlI CCTC 2 cut(s) 15, 77
MseI TTAA 1 cut(s) 355
MspA1I CMGCKG 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 119, 128, 304
NdeI CATATG 1 cut(s) 192
NdeII GATC 2 cut(s) 184, 247
NlaIII CATG 1 cut(s) 245
NlaIV GGNNCC 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 48
PkrI GCNGC 4 cut(s) 112, 152, 292, 337
PspN4I GGNNCC 1 cut(s) 171
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 171, 331
RsaNI GTAC 2 cut(s) 170, 330
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 355
SatI GCNGC 4 cut(s) 111, 151, 291, 336
Sau3AI GATC 2 cut(s) 184, 247
SetI ASST 7 cut(s) 13, 69, 112, 184, 213, 266, 349
SfaNI GCATC 1 cut(s) 109
SsiI CCGC 1 cut(s) 150
SspMI CTAG 2 cut(s) 8, 12
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 46, 93
TauI GCSGC 1 cut(s) 153
Tru1I TTAA 1 cut(s) 355
Tru9I TTAA 1 cut(s) 355
TseI GCWGC 3 cut(s) 110, 290, 335
TspDTI ATGAA 3 cut(s) 179, 209, 258
XspI CTAG 2 cut(s) 8, 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.