Rh1BG155700

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005206
Physical Location & Seq
Reverse (-)
25393097 .. 25393704
608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG155700.1

Sequence Viewer

Length: 312 bp
ATGGTTTCTCCTTCTCTTATTTTACTTCTTGTTCATGTTACTCACTCAGCTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTAAGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTTCTTCTTCACAGCTACAACAGAAGCTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTGCTGTCTTCCCAAGCAAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

103

Amino Acids

11.26

Weight (kDa)

6.26

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 25 - 96 1.2e-12 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AfiI CCNNNNNNNGG 1 cut(s) 104
AluBI AGCT 4 cut(s) 50, 191, 207, 219
AluI AGCT 4 cut(s) 50, 191, 207, 219
Alw26I GTCTC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 50, 233
AseI ATTAAT 1 cut(s) 168
Asp700I GAANNNNTTC 1 cut(s) 192
BanI GGYRCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 37, 220
BccI CCATC 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 141
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 2 cut(s) 51, 234
BlsI GCNGC 2 cut(s) 52, 235
BmiI GGNNCC 1 cut(s) 111
BmsI GCATC 1 cut(s) 49
BpiI GAAGAC 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 223
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 113, 270
Bse3DI GCAATG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 223
BseGI GGATG 2 cut(s) 64, 283
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 2 cut(s) 60, 68
BseNI ACTGG 2 cut(s) 113, 270
BseXI GCAGC 2 cut(s) 37, 220
BshNI GGYRCC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 141
BsmBI CGTCTC 1 cut(s) 141
Bsp143I GATC 1 cut(s) 124
Bsp19I CCATGG 1 cut(s) 223
BspCNI CTCAG 2 cut(s) 59, 67
BspLI GGNNCC 1 cut(s) 111
BspQI GCTCTTC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 243
BsrI ACTGG 2 cut(s) 113, 270
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 1 cut(s) 124
BssT1I CCWWGG 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 198
BstDEI CTNAG 3 cut(s) 46, 54, 143
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 64, 283
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 3 cut(s) 59, 68, 188
BstV1I GCAGC 2 cut(s) 37, 220
BstV2I GAAGAC 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 64, 283
CviAII CATG 4 cut(s) 35, 131, 185, 224
CviJI RGCY 7 cut(s) 50, 71, 97, 191, 207, 219, 269
CviKI_1 RGCY 7 cut(s) 50, 71, 97, 191, 207, 219, 269
DdeI CTNAG 3 cut(s) 46, 54, 143
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 223
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 4 cut(s) 38, 134, 188, 227
FaiI YATR 7 cut(s) 36, 75, 84, 86, 132, 186, 225
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FatI CATG 4 cut(s) 34, 130, 184, 223
Fnu4HI GCNGC 2 cut(s) 51, 234
FokI GGATG 2 cut(s) 71, 290
Fsp4HI GCNGC 2 cut(s) 51, 234
GluI GCNGC 2 cut(s) 51, 234
Hin1II CATG 4 cut(s) 38, 134, 188, 227
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 157
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 2 cut(s) 182, 236
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 68, 188
HpyF3I CTNAG 3 cut(s) 46, 54, 143
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 4 cut(s) 38, 134, 188, 227
Kzo9I GATC 1 cut(s) 124
LguI GCTCTTC 1 cut(s) 198
LpnPI CCDG 5 cut(s) 41, 126, 135, 251, 255
Lsp1109I GCAGC 2 cut(s) 37, 220
LweI GCATC 1 cut(s) 49
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 2 cut(s) 37, 115
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 5 cut(s) 119, 185, 188, 191, 285
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 50
MwoI GCNNNNNNNGC 3 cut(s) 59, 68, 188
NcoI CCATGG 1 cut(s) 223
NdeII GATC 1 cut(s) 124
NlaIII CATG 4 cut(s) 38, 134, 188, 227
NlaIV GGNNCC 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 192
PkrI GCNGC 2 cut(s) 52, 235
PshBI ATTAAT 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 2 cut(s) 51, 234
Sau3AI GATC 1 cut(s) 124
SetI ASST 6 cut(s) 52, 124, 153, 193, 209, 221
SfaNI GCATC 1 cut(s) 49
SspI AATATT 1 cut(s) 139
StyI CCWWGG 1 cut(s) 223
TaiI ACGT 1 cut(s) 153
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseI GCWGC 2 cut(s) 50, 233
TspDTI ATGAA 3 cut(s) 23, 119, 201
VspI ATTAAT 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.