Rh1DG076900

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005206
Physical Location & Seq
Reverse (-)
14185647 .. 14186140
494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG076900.1

Sequence Viewer

Length: 348 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCGGTATTCTATTGCTTGTCGATGTTACTCACTCAACTGTTCAAGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTATCCATATTACCTGATCTTCATGTGAATATGCTACAGAACGTAGTTGACAACAGCAACATTGATATTGCACATGAAGCGCTTCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATAACCGGTACTGCTGCAACCCTACCTTTGCATTGTATAATCTTACTATTCATACAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

115

Amino Acids

12.84

Weight (kDa)

6.17

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 97 1.5e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 39
AfaI GTAC 1 cut(s) 295
AfeI AGCGCT 1 cut(s) 213
AfiI CCNNNNNNNGG 1 cut(s) 128
AgeI ACCGGT 1 cut(s) 290
AgsI TTSAA 2 cut(s) 80, 244
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 1 cut(s) 228
AluI AGCT 1 cut(s) 228
Aor51HI AGCGCT 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 254, 299
AsiGI ACCGGT 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 241, 286
BccI CCATC 1 cut(s) 6
BciVI GTATCC 1 cut(s) 145
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 1 cut(s) 215
BfuI GTATCC 1 cut(s) 145
BglI GCCNNNNNGGC 1 cut(s) 92
BisI GCNGC 2 cut(s) 255, 300
BlsI GCNGC 2 cut(s) 256, 301
BmsI GCATC 1 cut(s) 73
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse118I RCCGGY 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 241, 286
BshTI ACCGGT 1 cut(s) 290
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 1 cut(s) 128
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 1 cut(s) 39
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 290
BssMI GATC 1 cut(s) 148
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 1 cut(s) 214
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 92, 209, 268
BstSFI CTRYAG 1 cut(s) 167
BstV1I GCAGC 2 cut(s) 241, 286
BsuI GTATCC 1 cut(s) 145
CfoI GCGC 1 cut(s) 214
Cfr10I RCCGGY 1 cut(s) 290
Csp6I GTAC 1 cut(s) 294
CspAI ACCGGT 1 cut(s) 290
CviAII CATG 2 cut(s) 155, 206
CviJI RGCY 4 cut(s) 5, 95, 121, 228
CviKI_1 RGCY 4 cut(s) 5, 95, 121, 228
CviQI GTAC 1 cut(s) 294
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco47III AGCGCT 1 cut(s) 213
FaeI CATG 2 cut(s) 158, 209
FaiI YATR 9 cut(s) 99, 108, 110, 140, 156, 164, 207, 323, 338
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FatI CATG 2 cut(s) 154, 205
Fnu4HI GCNGC 2 cut(s) 255, 300
Fsp4HI GCNGC 2 cut(s) 255, 300
GlaI GCGC 1 cut(s) 213
GluI GCNGC 2 cut(s) 255, 300
HaeII RGCGCY 1 cut(s) 215
HapII CCGG 1 cut(s) 291
HhaI GCGC 1 cut(s) 214
Hin1II CATG 2 cut(s) 158, 209
Hin6I GCGC 1 cut(s) 212
HinP1I GCGC 1 cut(s) 212
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HpaII CCGG 1 cut(s) 291
Hpy166II GTNNAC 1 cut(s) 181
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 203, 257, 302, 316
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 209, 268
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 2 cut(s) 158, 209
HspAI GCGC 1 cut(s) 212
Kzo9I GATC 1 cut(s) 148
LguI GCTCTTC 1 cut(s) 12
LpnPI CCDG 3 cut(s) 159, 304, 326
Lsp1109I GCAGC 2 cut(s) 241, 286
LweI GCATC 1 cut(s) 73
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 3 cut(s) 143, 209, 212
MnlI CCTC 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 213
MspI CCGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 92, 209, 268
NdeII GATC 1 cut(s) 148
NlaIII CATG 2 cut(s) 158, 209
PciSI GCTCTTC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 213
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 2 cut(s) 256, 301
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 2 cut(s) 255, 300
Sau3AI GATC 1 cut(s) 148
SetI ASST 5 cut(s) 33, 148, 177, 230, 313
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 167
SsiI CCGC 1 cut(s) 39
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 10, 57
TseI GCWGC 2 cut(s) 254, 299
TspDTI ATGAA 3 cut(s) 143, 222, 325
XmnI GAANNNNTTC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.