Rh1DG350900

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005206
Physical Location & Seq
Reverse (-)
57550588 .. 57554163
3576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG350900.1

Sequence Viewer

Length: 339 bp
ATGGCTCTTCGATGGTTTCTCCTCCTTAGCCTCATTGGCAGTATTCTATTTCTTGTTGATGTTACTCACTCAGCTGCTCGGGATGCCCGAAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGATGCTAGTAACACCTGATCTTCATGTAAATATTCTACGAGACATAGTTGATAGCAGCATTCATAAGGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCACGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGCTGGTACTCATACAGGCACTGATGATGGCATGGAAGAGATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

112

Amino Acids

12.55

Weight (kDa)

6.12

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 99 1.5e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 128
AluBI AGCT 3 cut(s) 74, 215, 231
AluI AGCT 3 cut(s) 74, 215, 231
Alw26I GTCTC 1 cut(s) 165
AlwNI CAGNNNCTG 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 74, 186, 257
AvaI CYCGRG 1 cut(s) 78
BbvI GCAGC 3 cut(s) 61, 198, 244
BccI CCATC 2 cut(s) 6, 311
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 1 cut(s) 137
BglI GCCNNNNNGGC 2 cut(s) 36, 92
BisI GCNGC 3 cut(s) 75, 187, 258
BlsI GCNGC 3 cut(s) 76, 188, 259
BmeT110I CYCGRG 1 cut(s) 78
BmsI GCATC 3 cut(s) 73, 123, 321
Bpu10I CCTNAGC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 2 cut(s) 88, 138
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 3 cut(s) 61, 198, 244
BsiHKCI CYCGRG 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 165
BsmI GAATGC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 78
Bsp143I GATC 1 cut(s) 148
BspCNI CTCAG 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 267
BsrI ACTGG 1 cut(s) 294
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 1 cut(s) 148
Bst6I CTCTTC 2 cut(s) 12, 321
BstDEI CTNAG 2 cut(s) 26, 70
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 2 cut(s) 88, 138
BstKTI GATC 1 cut(s) 151
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 4 cut(s) 36, 83, 92, 212
BstV1I GCAGC 3 cut(s) 61, 198, 244
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 88, 138
BtsIMutI CAGTG 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 311
Csp6I GTAC 1 cut(s) 297
CviAII CATG 3 cut(s) 155, 209, 322
CviJI RGCY 8 cut(s) 5, 30, 74, 95, 121, 215, 231, 293
CviKI_1 RGCY 8 cut(s) 5, 30, 74, 95, 121, 215, 231, 293
CviQI GTAC 1 cut(s) 297
DdeI CTNAG 2 cut(s) 26, 70
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 12, 321
EarI CTCTTC 2 cut(s) 12, 321
Eco88I CYCGRG 1 cut(s) 78
FaeI CATG 3 cut(s) 158, 212, 325
FaiI YATR 9 cut(s) 99, 108, 110, 156, 176, 195, 210, 303, 323
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 3 cut(s) 154, 208, 321
Fnu4HI GCNGC 3 cut(s) 75, 187, 258
FokI GGATG 2 cut(s) 95, 145
Fsp4HI GCNGC 3 cut(s) 75, 187, 258
FspBI CTAG 1 cut(s) 137
GluI GCNGC 3 cut(s) 75, 187, 258
Hin1II CATG 3 cut(s) 158, 212, 325
Hpy188III TCNNGA 1 cut(s) 80
HpyCH4V TGCA 3 cut(s) 206, 260, 334
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 83, 92, 212
HpyF3I CTNAG 2 cut(s) 26, 70
Hsp92II CATG 3 cut(s) 158, 212, 325
Kzo9I GATC 1 cut(s) 148
LguI GCTCTTC 1 cut(s) 12
LpnPI CCDG 4 cut(s) 159, 275, 279, 291
Lsp1109I GCAGC 3 cut(s) 61, 198, 244
LweI GCATC 3 cut(s) 73, 123, 321
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 2 cut(s) 61, 139
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 3 cut(s) 143, 215, 338
MnlI CCTC 2 cut(s) 32, 41
MspA1I CMGCKG 1 cut(s) 74
Mva1269I GAATGC 1 cut(s) 189
MwoI GCNNNNNNNGC 4 cut(s) 36, 83, 92, 212
NdeII GATC 1 cut(s) 148
NlaIII CATG 3 cut(s) 158, 212, 325
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PctI GAATGC 1 cut(s) 189
PkrI GCNGC 3 cut(s) 76, 188, 259
PstNI CAGNNNCTG 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 298
RsaNI GTAC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 3 cut(s) 75, 187, 258
Sau3AI GATC 1 cut(s) 148
SetI ASST 4 cut(s) 76, 148, 217, 233
SfaNI GCATC 3 cut(s) 73, 123, 321
SspI AATATT 1 cut(s) 163
SspMI CTAG 1 cut(s) 137
TaqI TCGA 1 cut(s) 10
TscAI CASTG 1 cut(s) 316
TseI GCWGC 3 cut(s) 74, 186, 257
TspDTI ATGAA 3 cut(s) 143, 182, 225
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 316
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.