Rmu_sc0000078.1_g000035

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000078.1
OG0001935
Physical Location & Seq
Forward (+)
157015 .. 159086
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
atggaacttgttcaaagtgtggcctctaccatgtccctaatccatggtgacattgatgataacgatgatgatggtccaaatctcaatgtttctcttccacaatgtgcatatttgcttggtgaaactgctggtgttatgaattggaccctttcggcgattaatcagcaatatactcaaggtgatgccatttcccatgcgaggacactgttgagtggtagtcttggcagccaagaaatgtgtacgcaaacgcttgatggtaccaaacatttcatggtttcaaggaggctcaaacaagtgaccacatccatatatgatatcctagatatgttgcaagtgccacgatttggtcctgatttttccagggccactggtgcttctttacttggttcgactagattcgacggcgttaagatttcacaaaaaccaaacgtcattgtttcacaagacgggaagggagatttcaagctaataatggacgcgattaaggaagcaccaagtcactccacaaattactttgtgatacttgttaagagaggagtttaccatgaatacgtgaacattgatgagacaaaatcgaatctggtcttgatgggagagggcatggatgttactaccatttccggaagtaaaagcgtaaggggtggtcagccaacatttgatacagccacatttgccgttagggccctaggattcattgcaatggacatagggtttgagaacactgccggacaagagaacatgcaggcgttggcggttcgctccagcggcgaccgctccgccttttaccgatgcaagatgaccggaaatcaagacacattgctagtgcaagctggccgccaattcttccgagaatgcagcatcacgggcaccgttgacttcatctttggctatggcagtgctgtctttcaaaaatgcgacatttacatcaagaaaaatccaataggcggaaccagcgtcatcgcggcccatggccgtaacaacccatcagacccatcaggcttttcattccacctctgcaccatcaccggagaccccaatgtggtcattaaagactcgactaacaacggagcgtacctcggtcggaattggggggtttatgcacgaacagtgttcatagattcgaccataagcaatgttctacggccggaaggttggctccagtggcaaggcaagggtgtggacaaagtgtatttcggggagtacatgaacaatggcccgggtgcaaacgctgcaaaccgggtgagctggcctggttgtcatgcgaggatgagccgatctgaggctgatagatttacagtggcaaagcttattgatggaccttcatggctgtcaactgtaggtatcccaaacacaatgggattggatggatcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
PMEI Pectinesterase

Protein Analysis

457

Amino Acids

49.62

Weight (kDa)

6.08

Isoelectric Point (pI)

27.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 143 - 439 9.5e-111 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 257
AccB1I GGYRCC 2 cut(s) 257, 866
AccB7I CCANNNNNTGG 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 774
AccII CGCG 2 cut(s) 479, 962
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 7 cut(s) 752, 765, 772, 777, 835, 945, 962
AclWI GGATC 1 cut(s) 1362
AcoI YGGCCR 3 cut(s) 832, 970, 1142
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 4 cut(s) 241, 259, 1073, 1202
AfiI CCNNNNNNNGG 5 cut(s) 198, 344, 944, 1039, 1279
AgsI TTSAA 4 cut(s) 14, 279, 463, 908
AjnI CCWGG 2 cut(s) 359, 1249
AluBI AGCT 4 cut(s) 466, 830, 1245, 1306
AluI AGCT 4 cut(s) 466, 830, 1245, 1306
Alw26I GTCTC 2 cut(s) 560, 1023
AlwI GGATC 1 cut(s) 1362
Ama87I CYCGRG 1 cut(s) 1216
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 9 cut(s) 21, 363, 681, 832, 963, 970, 1142, 1213, 1247
ApaI GGGCCC 1 cut(s) 685
ApeKI GCWGC 3 cut(s) 225, 855, 1229
ArsI GACNNNNNNTTYG 4 cut(s) 695, 727, 866, 898
AseI ATTAAT 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 9
Asp718I GGTACC 1 cut(s) 257
AspA2I CCTAGG 1 cut(s) 685
AspS9I GGNCC 9 cut(s) 74, 144, 347, 363, 681, 682, 964, 1214, 1316
AsuC2I CCSGG 3 cut(s) 1217, 1218, 1238
AsuHPI GGTGA 5 cut(s) 59, 131, 191, 1015, 1252
AvaI CYCGRG 1 cut(s) 1216
AvaII GGWCC 4 cut(s) 74, 144, 347, 1316
AvrII CCTAGG 1 cut(s) 685
BaeGI GKGCMC 2 cut(s) 685, 869
BamHI GGATCC 1 cut(s) 1367
BanI GGYRCC 2 cut(s) 257, 866
BanII GRGCYC 1 cut(s) 685
BbvI GCAGC 3 cut(s) 237, 867, 1216
BccI CCATC 8 cut(s) 65, 248, 583, 991, 1000, 1028, 1307, 1358
BceAI ACGGC 4 cut(s) 418, 659, 957, 1157
BciT130I CCWGG 2 cut(s) 361, 1251
BciVI GTATCC 1 cut(s) 1352
BcnI CCSGG 3 cut(s) 1217, 1218, 1238
BcoDI GTCTC 2 cut(s) 560, 1023
BfaI CTAG 4 cut(s) 320, 393, 686, 821
BfmI CTRYAG 1 cut(s) 1335
BfuI GTATCC 1 cut(s) 1352
BglI GCCNNNNNGGC 1 cut(s) 680
BisI GCNGC 6 cut(s) 226, 766, 835, 856, 963, 1230
BlnI CCTAGG 1 cut(s) 685
BlsI GCNGC 6 cut(s) 227, 767, 836, 857, 964, 1231
Bme1390I CCNGG 5 cut(s) 361, 1217, 1218, 1238, 1251
Bme18I GGWCC 4 cut(s) 74, 144, 347, 1316
BmeT110I CYCGRG 1 cut(s) 1216
BmgT120I GGNCC 9 cut(s) 74, 144, 347, 363, 681, 682, 964, 1214, 1316
BmiI GGNNCC 7 cut(s) 146, 259, 683, 868, 949, 1157, 1369
BmrFI CCNGG 5 cut(s) 361, 1217, 1218, 1238, 1251
BmsI GCATC 3 cut(s) 172, 779, 867
BplI GAGNNNNNCTC 2 cut(s) 1059, 1091
BpmI CTGGAG 2 cut(s) 745, 1142
BpuEI CTTGAG 1 cut(s) 159
BpuMI CCSGG 3 cut(s) 1217, 1218, 1238
BsaAI YACGTR 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 1023
BsaJI CCNNGG 6 cut(s) 43, 360, 685, 967, 1075, 1216
BsaWI WCCGGW 3 cut(s) 620, 800, 1025
Bsc4I CCNNNNNNNGG 5 cut(s) 198, 344, 944, 1039, 1279
Bse1I ACTGG 2 cut(s) 373, 1159
Bse3DI GCAATG 4 cut(s) 693, 705, 815, 1138
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 2 cut(s) 361, 1251
BseDI CCNNGG 6 cut(s) 43, 360, 685, 967, 1075, 1216
BseGI GGATG 4 cut(s) 302, 610, 1272, 1369
BseLI CCNNNNNNNGG 5 cut(s) 198, 344, 944, 1039, 1279
BseMI GCAATG 4 cut(s) 693, 705, 815, 1138
BseMII CTCAG 1 cut(s) 1269
BseNI ACTGG 2 cut(s) 373, 1159
BseRI GAGGAG 1 cut(s) 549
BseSI GKGCMC 2 cut(s) 685, 869
BseX3I CGGCCG 1 cut(s) 1142
BseXI GCAGC 3 cut(s) 237, 867, 1216
BsgI GTGCAG 1 cut(s) 1000
Bsh1236I CGCG 2 cut(s) 479, 962
Bsh1285I CGRYCG 3 cut(s) 772, 1081, 1145
BshFI GGCC 9 cut(s) 23, 365, 683, 834, 965, 972, 1144, 1215, 1249
BshNI GGYRCC 2 cut(s) 257, 866
BsiEI CGRYCG 3 cut(s) 772, 1081, 1145
BsiHKCI CYCGRG 1 cut(s) 1216
BsiSI CCGG 7 cut(s) 621, 726, 801, 1026, 1145, 1217, 1237
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 5 cut(s) 198, 344, 944, 1039, 1279
BsmAI GTCTC 2 cut(s) 560, 1023
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 857
BsnI GGCC 9 cut(s) 23, 365, 683, 834, 965, 972, 1144, 1215, 1249
Bso31I GGTCTC 1 cut(s) 1023
BsoBI CYCGRG 1 cut(s) 1216
Bsp120I GGGCCC 1 cut(s) 681
Bsp1286I GDGCHC 2 cut(s) 685, 869
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 2 cut(s) 1274, 1367
Bsp19I CCATGG 2 cut(s) 43, 967
BspACI CCGC 7 cut(s) 752, 765, 772, 777, 835, 945, 962
BspANI GGCC 9 cut(s) 23, 365, 683, 834, 965, 972, 1144, 1215, 1249
BspCNI CTCAG 1 cut(s) 1270
BspEI TCCGGA 1 cut(s) 620
BspFNI CGCG 2 cut(s) 479, 962
BspLI GGNNCC 7 cut(s) 146, 259, 683, 868, 949, 1157, 1369
BspPI GGATC 1 cut(s) 1362
BspT107I GGYRCC 2 cut(s) 257, 866
BspTNI GGTCTC 1 cut(s) 1023
BsrBI CCGCTC 1 cut(s) 774
BsrDI GCAATG 4 cut(s) 693, 705, 815, 1138
BsrI ACTGG 2 cut(s) 373, 1159
BssECI CCNNGG 6 cut(s) 43, 360, 685, 967, 1075, 1216
BssMI GATC 2 cut(s) 1274, 1367
BssT1I CCWWGG 3 cut(s) 43, 685, 967
Bst2UI CCWGG 2 cut(s) 361, 1251
Bst4CI ACNGT 5 cut(s) 207, 871, 1108, 1297, 1336
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstBAI YACGTR 1 cut(s) 553
BstC8I GCNNGC 4 cut(s) 744, 828, 832, 1247
BstDEI CTNAG 1 cut(s) 1278
BstDSI CCRYGG 2 cut(s) 43, 967
BstF5I GGATG 4 cut(s) 302, 610, 1272, 1369
BstFNI CGCG 2 cut(s) 479, 962
BstKTI GATC 2 cut(s) 1277, 1370
BstMAI GTCTC 2 cut(s) 560, 1023
BstMBI GATC 2 cut(s) 1274, 1367
BstMCI CGRYCG 3 cut(s) 772, 1081, 1145
BstMWI GCNNNNNNNGC 9 cut(s) 371, 671, 680, 765, 771, 864, 951, 1162, 1229
BstNI CCWGG 2 cut(s) 361, 1251
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 5 cut(s) 359, 1215, 1216, 1236, 1249
BstSFI CTRYAG 1 cut(s) 1335
BstSLI GKGCMC 2 cut(s) 685, 869
BstUI CGCG 2 cut(s) 479, 962
BstV1I GCAGC 3 cut(s) 237, 867, 1216
BstX2I RGATCY 1 cut(s) 1367
BstYI RGATCY 1 cut(s) 1367
BstZI CGGCCG 1 cut(s) 1142
BsuI GTATCC 1 cut(s) 1352
BsuRI GGCC 9 cut(s) 23, 365, 683, 834, 965, 972, 1144, 1215, 1249
BtgI CCRYGG 2 cut(s) 43, 967
BtgZI GCGATG 1 cut(s) 943
BtsCI GGATG 4 cut(s) 302, 610, 1272, 1369
BtsI GCAGTG 2 cut(s) 720, 901
BtsIMutI CAGTG 7 cut(s) 203, 366, 720, 901, 1113, 1166, 1302
Cac8I GCNNGC 4 cut(s) 744, 828, 832, 1247
Cfr13I GGNCC 9 cut(s) 74, 144, 347, 363, 681, 682, 964, 1214, 1316
Cfr9I CCCGGG 1 cut(s) 1216
CseI GACGC 2 cut(s) 485, 943
Csp6I GTAC 4 cut(s) 240, 258, 1072, 1201
CviQI GTAC 4 cut(s) 240, 258, 1072, 1201
DdeI CTNAG 1 cut(s) 1278
DpnI GATC 2 cut(s) 1276, 1369
DpnII GATC 2 cut(s) 1274, 1367
DraIII CACNNNGTG 1 cut(s) 104
EaeI YGGCCR 3 cut(s) 832, 970, 1142
EagI CGGCCG 1 cut(s) 1142
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 2 cut(s) 766, 960
EclXI CGGCCG 1 cut(s) 1142
Eco130I CCWWGG 3 cut(s) 43, 685, 967
Eco24I GRGCYC 1 cut(s) 685
Eco31I GGTCTC 1 cut(s) 1023
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 4 cut(s) 74, 144, 347, 1316
Eco52I CGGCCG 1 cut(s) 1142
Eco88I CYCGRG 1 cut(s) 1216
EcoO109I RGGNCCY 2 cut(s) 681, 682
EcoRII CCWGG 2 cut(s) 359, 1249
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 43, 685, 967
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 3 cut(s) 43, 685, 967
FaqI GGGAC 1 cut(s) 19
Fnu4HI GCNGC 6 cut(s) 226, 766, 835, 856, 963, 1230
FokI GGATG 3 cut(s) 289, 617, 1279
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 6 cut(s) 226, 766, 835, 856, 963, 1230
FspBI CTAG 4 cut(s) 320, 393, 686, 821
GluI GCNGC 6 cut(s) 226, 766, 835, 856, 963, 1230
GsuI CTGGAG 2 cut(s) 745, 1142
HaeIII GGCC 9 cut(s) 23, 365, 683, 834, 965, 972, 1144, 1215, 1249
HapII CCGG 7 cut(s) 621, 726, 801, 1026, 1145, 1217, 1237
HgaI GACGC 2 cut(s) 485, 943
HincII GTYRAC 2 cut(s) 874, 1332
HindII GTYRAC 2 cut(s) 874, 1332
HindIII AAGCTT 1 cut(s) 1304
HinfI GANTC 5 cut(s) 396, 577, 690, 1052, 1118
HpaII CCGG 7 cut(s) 621, 726, 801, 1026, 1145, 1217, 1237
HphI GGTGA 5 cut(s) 59, 131, 191, 1015, 1252
Hpy166II GTNNAC 6 cut(s) 240, 541, 556, 874, 1180, 1332
Hpy188I TCNGA 4 cut(s) 848, 988, 1083, 1279
Hpy188III TCNNGA 5 cut(s) 350, 586, 621, 809, 928
Hpy8I GTNNAC 6 cut(s) 240, 541, 556, 874, 1180, 1332
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 3 cut(s) 445, 1142, 1329
HpyCH4III ACNGT 5 cut(s) 207, 871, 1108, 1297, 1336
HpyCH4IV ACGT 2 cut(s) 429, 552
HpyF10VI GCNNNNNNNGC 9 cut(s) 371, 671, 680, 765, 771, 864, 951, 1162, 1229
HpyF3I CTNAG 1 cut(s) 1278
HpySE526I ACGT 2 cut(s) 429, 552
Kpn2I TCCGGA 1 cut(s) 620
KpnI GGTACC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 1274, 1367
LmnI GCTCC 4 cut(s) 764, 779, 1067, 1161
Lsp1109I GCAGC 3 cut(s) 237, 867, 1216
LweI GCATC 3 cut(s) 172, 779, 867
MaeI CTAG 4 cut(s) 320, 393, 686, 821
MaeII ACGT 2 cut(s) 429, 552
MaeIII GTNAC 5 cut(s) 47, 295, 497, 607, 974
MalI GATC 2 cut(s) 1276, 1369
MbiI CCGCTC 1 cut(s) 774
MboI GATC 2 cut(s) 1274, 1367
MboII GAAGA 2 cut(s) 86, 835
MflI RGATCY 1 cut(s) 1367
MhlI GDGCHC 2 cut(s) 685, 869
MluCI AATT 4 cut(s) 139, 508, 839, 1084
MlyI GAGTC 1 cut(s) 1046
MmeI TCCRAC 1 cut(s) 1061
MnlI CCTC 9 cut(s) 34, 192, 276, 527, 589, 1022, 1085, 1257, 1273
MroI TCCGGA 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 5 cut(s) 159, 408, 483, 528, 1047
MslI CAYNNNNRTG 1 cut(s) 698
MspA1I CMGCKG 1 cut(s) 765
MspI CCGG 7 cut(s) 621, 726, 801, 1026, 1145, 1217, 1237
MspR9I CCNGG 5 cut(s) 361, 1217, 1218, 1238, 1251
Mva1269I GAATGC 1 cut(s) 857
MvaI CCWGG 2 cut(s) 361, 1251
MvnI CGCG 2 cut(s) 479, 962
MwoI GCNNNNNNNGC 9 cut(s) 371, 671, 680, 765, 771, 864, 951, 1162, 1229
NciI CCSGG 3 cut(s) 1217, 1218, 1238
NcoI CCATGG 2 cut(s) 43, 967
NdeII GATC 2 cut(s) 1274, 1367
NlaIV GGNNCC 7 cut(s) 146, 259, 683, 868, 949, 1157, 1369
NmuCI GTSAC 3 cut(s) 47, 295, 497
NspI RCATGY 1 cut(s) 742
PctI GAATGC 1 cut(s) 857
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 4 cut(s) 396, 577, 690, 1118
PflMI CCANNNNNTGG 1 cut(s) 344
PkrI GCNGC 6 cut(s) 227, 767, 836, 857, 964, 1231
PleI GAGTC 1 cut(s) 1046
PpsI GAGTC 1 cut(s) 1046
Ppu21I YACGTR 1 cut(s) 553
PshBI ATTAAT 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 359, 1249
PspGI CCWGG 2 cut(s) 359, 1249
PspN4I GGNNCC 7 cut(s) 146, 259, 683, 868, 949, 1157, 1369
PspOMI GGGCCC 1 cut(s) 681
PspPI GGNCC 9 cut(s) 74, 144, 347, 363, 681, 682, 964, 1214, 1316
PsuI RGATCY 1 cut(s) 1367
RsaI GTAC 4 cut(s) 241, 259, 1073, 1202
RsaNI GTAC 4 cut(s) 240, 258, 1072, 1201
RseI CAYNNNNRTG 1 cut(s) 698
SaqAI TTAA 5 cut(s) 159, 408, 483, 528, 1047
SatI GCNGC 6 cut(s) 226, 766, 835, 856, 963, 1230
Sau3AI GATC 2 cut(s) 1274, 1367
Sau96I GGNCC 9 cut(s) 74, 144, 347, 363, 681, 682, 964, 1214, 1316
SchI GAGTC 1 cut(s) 1046
ScrFI CCNGG 5 cut(s) 361, 1217, 1218, 1238, 1251
SduI GDGCHC 2 cut(s) 685, 869
SfaNI GCATC 3 cut(s) 172, 779, 867
SfcI CTRYAG 1 cut(s) 1335
SinI GGWCC 4 cut(s) 74, 144, 347, 1316
SmaI CCCGGG 1 cut(s) 1218
SmiMI CAYNNNNRTG 1 cut(s) 698
SmlI CTYRAG 1 cut(s) 174
SmoI CTYRAG 1 cut(s) 174
Sse9I AATT 4 cut(s) 139, 508, 839, 1084
SsiI CCGC 7 cut(s) 752, 765, 772, 777, 835, 945, 962
SspMI CTAG 4 cut(s) 320, 393, 686, 821
StyD4I CCNGG 5 cut(s) 359, 1215, 1216, 1236, 1249
StyI CCWWGG 3 cut(s) 43, 685, 967
TaaI ACNGT 5 cut(s) 207, 871, 1108, 1297, 1336
TaiI ACGT 2 cut(s) 432, 555
TaqI TCGA 5 cut(s) 389, 399, 575, 1055, 1121
TaqII GACCGA 1 cut(s) 1067
TasI AATT 4 cut(s) 139, 508, 839, 1084
TatI WGTACW 1 cut(s) 1200
TauI GCSGC 3 cut(s) 768, 837, 965
TfiI GAWTC 4 cut(s) 396, 577, 690, 1118
Tru1I TTAA 5 cut(s) 159, 408, 483, 528, 1047
Tru9I TTAA 5 cut(s) 159, 408, 483, 528, 1047
TscAI CASTG 7 cut(s) 210, 373, 727, 901, 1113, 1166, 1302
TseFI GTSAC 3 cut(s) 47, 295, 497
TseI GCWGC 3 cut(s) 225, 855, 1229
Tsp45I GTSAC 3 cut(s) 47, 295, 497
TspDTI ATGAA 9 cut(s) 152, 259, 561, 682, 868, 993, 1102, 1220, 1311
TspGWI ACGGA 1 cut(s) 1080
TspMI CCCGGG 1 cut(s) 1216
TspRI CASTG 7 cut(s) 210, 373, 727, 901, 1113, 1166, 1302
Van91I CCANNNNNTGG 1 cut(s) 344
VpaK11BI GGWCC 4 cut(s) 74, 144, 347, 1316
VspI ATTAAT 1 cut(s) 159
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XmaI CCCGGG 1 cut(s) 1216
XmaJI CCTAGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 4 cut(s) 320, 393, 686, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.