Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000221.1

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

84161 .. 89888

Gene Structure 5728 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

atggcgaccaagttctttgctcttgcgtgcatacgcaacaacaacagtggtgaaggccgcggcggcagcgatttgtctccgcggcctcactacccctccatgccgaagtatccgaagggtgtggtggccgaggagaccactatggtggtgggcaccgagtccaaggccgtgttctccgtcatcggcatgacctgctccgcctgcgcgggatcggtagagaaggctgtgaagaggctacccgggattcgcgaggcctgctgcgtttttcatttcatggcaaccaagttctttgctcttgcgtgcatacgcaacaacaacagtggtgaaggccgcggcggcagcgatttgtctccgcggcctcactacccctccatgccgaagtatccgaagggtgtggtggccgaggagaccactatggtggtgggcaccgagtccaaggccgtgttctccgtcatcggcatgacctgctccgcctgcgcgggatcggtagagaaggctgtgaagaggctacccgggattcgcgaggcggtggtcgacgtgctcaacaaccgtgatccagtcccatgtaacaaacatagccttactttactctttctttgcaagactatccgtgagactattgaagatgttggatttcaagcaacattgattgctgatgagggaaacgagaagcccacgttggtgtgcagaataaggatcaaaggaatgacttgcacttcttgctcttccactgttgaatcagctttgcaggcagttcatggtgtacaaaaagcccaagttgccttagcaactgaagaagcagatgtccactatgatccaaagatagtgagctgcaatcagctgatggtagccattgaagacactggattcgaagccatacttattaattcaggtgaagacatgagcaagatagatcttaaagttgacggtgtaaggaccgatcattcaatgagaatacttgaacagtctcttcaagcactccctggggtgcaaggtgtagacatacatcatgatgacagaaaaatttccctgtcttacaagccagatatgacagggccccgaaatttcatcaatgtgattgaaacaactggatccaggcgtttcagggcaaaaatttatccaggcggtggagcaggaagagaaaatcataggaaggaggaaattcagcagtactacagagtctttctctggagtttgattttcaccattccagtatttttaacctccatggtcttcatgtacatccctggacttaagcatggcttagacaagaaagtagtaaatatgctcagcattggggagcttataaggtggattctgtctactccagtgcagttcatcataggcctgaggttctatactggggcatacaagtcattgcgccatggttctgctaatatggatgtgttaattgccttgggaacaaatgctgcatatttctattcagtctactccgtactgagagctgccacctccccacattttaagggtacagatttctttgagactagtgcaatgctaatctcattcattctcttagggaagtacctagaggtgttagctaaggggaagacatctgatgctattgccaaactgatggatttggcacctgaaacagccactttgttgactctagacgaagaaagaaacattttaggtgaagaagaaattgatggtcggttgatacaaaagaacgatgtcatcaaaattattccaggggcgaaggtatcttcagatggttatgtgatatggggacaaagccatataaatgagagtatgataacaggagaagcacgtccagtagcaaaaaggaagggtgatacagtcattggaggtactgtaaatgaaaatggtgtgttgcatatcaaggcaacacgggttggatcagaaagttccctcgcacagattgtacggcttgttgagtcagctcagatggccaaagctcctgttcagaagtttgctgaccgcatttctaaattctttgtgccactggtgatcatgctttcattctttacctggctttcatggtttttggctggaaaattccacggctacccgaaatcttggataccgaagtccatggatagctttcagcttgctctccagtttgggatctctgtcatggttattgcgtgcccttgtgctctaggcctagcaactcctactgctgtcatggttggtactggagttggtgcctcccaaggtgtattgatcaaaggaggtcaagcattagaaagtgcacataaggtggactgcattgtgttcgacaagacagggactcttacaattgggaagccactggttgttaacacacgactcctgaaaaatatggtgcttcgagatttctatgaacttgttgctgcagctgaggttaacagtgaacaccccttggccaaggccattgttgagtatgccaaaaaatttagagaagatgaagagaatccagccggcccagaagcgcatgactttgtctccattactggtcacggggtgaaggctattgttcggggcagggaaataattgtggggaacaagagcttgatggtggaccagaacattgcagttccactcgatgcagaagactacctagcagaagctgaagggctggctcaaactgggattctagtagccatagatggagaagtggccggagttctagcaatatctgatccactgaaaccagcgaaacctgatcaccagaaagcagagaaagtgaaggaattgcaggcttcaggctacactgtggcgacggtgggagatggcatcaatgactcaccagcacttgtggcagcagatgtaggaatggcaattggtgctggcactgacattgcaattgaggcagcagacattgtgctaatgaagagtaacttggaggatgtgataactgccattgatctttcaaggaaaaccttcacccgtattcgtttgaactacatttgggctttgggatataatgtgctcgggatcccaatagctgcaggagttctcttcccatccactggatttcgtttacccccttggattgctggagctgcaatggctgcttcttctgtcagtgttgtttgctgttcattgttgttgaagaactataagagacccaaggagctgaataaccttgaggtgcgtggaatagtgatcgagtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

1047

Amino Acids

113.26

Weight (kDa)

7.76

Isoelectric Point (pI)

33.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	57 - 85	1.9e-06	Heavy-metal-associated domain
HMA	PF00403	148 - 211	9.1e-10	Heavy-metal-associated domain
HMA	PF00403	231 - 291	3.4e-13	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	536 - 641	6e-23	P-type ATPase actuator domain
Hydrolase	PF00702	750 - 894	6.2e-10	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1307
Acc36I	ACCTGC	2 cut(s)	200, 473
AccB1I	GGYRCC	4 cut(s)	152, 425, 1606, 2192
AccB7I	CCANNNNNTGG	2 cut(s)	1127, 2999
AccI	GTMKAC	4 cut(s)	534, 999, 1322, 1449
AccII	CGCG	8 cut(s)	60, 82, 206, 249, 333, 355, 479, 522
AcoI	YGGCCR	5 cut(s)	126, 399, 1935, 2391, 2646
AcsI	RAATTY	7 cut(s)	1023, 1063, 1113, 1161, 1976, 2042, 2420
AcuI	CTGAAG	4 cut(s)	813, 1716, 2619, 2715
AdeI	CACNNNGTG	1 cut(s)	2491
AfaI	GTAC	9 cut(s)	765, 1172, 1241, 1458, 1492, 1547, 1837, 1911, 2182
AfiI	CCNNNNNNNGG	2 cut(s)	1127, 2999
AflII	CTTAAG	1 cut(s)	1253
AhlI	ACTAGT	1 cut(s)	1508
AjiI	CACGTC	2 cut(s)	538, 1796
AjnI	CCWGG	6 cut(s)	982, 1094, 1120, 1246, 1714, 2015
AjuI	GAANNNNNNNTTGG	8 cut(s)	155, 187, 428, 460, 662, 694, 2852, 2884
AleI	CACNNNNGTG	3 cut(s)	143, 416, 680
Alw21I	GWGCWC	4 cut(s)	543, 2146, 2242, 2961
Alw26I	GTCTC	9 cut(s)	81, 128, 354, 401, 608, 972, 1499, 2476, 3088
Alw44I	GTGCAC	1 cut(s)	2238
AlwNI	CAGNNNCTG	1 cut(s)	2597
Ama87I	CYCGRG	3 cut(s)	239, 512, 2960
ApaI	GGGCCC	1 cut(s)	1059
ApaLI	GTGCAC	1 cut(s)	2238
ApoI	RAATTY	7 cut(s)	1023, 1063, 1113, 1161, 1976, 2042, 2420
ArsI	GACNNNNNNTTYG	2 cut(s)	851, 883
AseI	ATTAAT	1 cut(s)	885
Asp700I	GAANNNNTTC	2 cut(s)	1024, 2909
AspLEI	GCGC	4 cut(s)	206, 479, 1383, 2461
AspS9I	GGNCC	5 cut(s)	936, 1055, 1056, 2450, 2548
AsuC2I	CCSGG	4 cut(s)	240, 241, 513, 514
AsuII	TTCGAA	1 cut(s)	870
AvaI	CYCGRG	3 cut(s)	239, 512, 2960
AvaII	GGWCC	2 cut(s)	936, 2548
AxyI	CCTNAGG	1 cut(s)	1349
BaeGI	GKGCMC	5 cut(s)	155, 428, 1059, 2138, 2242
BaeI	ACNNNNGTAYC	4 cut(s)	2060, 2093, 2172, 2205
BalI	TGGCCA	2 cut(s)	1937, 2393
BamHI	GGATCC	2 cut(s)	1091, 2964
BanI	GGYRCC	4 cut(s)	152, 425, 1606, 2192
BanII	GRGCYC	1 cut(s)	1059
BbsI	GAAGAC	5 cut(s)	864, 903, 1225, 1577, 2586
Bbv12I	GWGCWC	4 cut(s)	543, 2146, 2242, 2961
BbvCI	CCTCAGC	1 cut(s)	2367
BccI	CCATC	9 cut(s)	838, 1591, 1667, 1730, 1927, 2536, 2630, 2753, 3001
BceAI	ACGGC	4 cut(s)	152, 425, 1928, 2065
BcgI	CGANNNNNNTGC	2 cut(s)	2245, 2279
BciT130I	CCWGG	6 cut(s)	984, 1096, 1122, 1248, 1716, 2017
BciVI	GTATCC	3 cut(s)	120, 393, 2061
BclI	TGATCA	3 cut(s)	1995, 2211, 2692
BcnI	CCSGG	4 cut(s)	240, 241, 513, 514
BcoDI	GTCTC	9 cut(s)	81, 128, 354, 401, 608, 972, 1499, 2476, 3088
BcuI	ACTAGT	1 cut(s)	1508
BfaI	CTAG	8 cut(s)	1509, 1550, 1634, 2147, 2153, 2588, 2624, 2657
BfmI	CTRYAG	3 cut(s)	1174, 2361, 2976
BfrI	CTTAAG	1 cut(s)	1253
BfuAI	ACCTGC	2 cut(s)	200, 473
BfuI	GTATCC	3 cut(s)	120, 393, 2061
BglI	GCCNNNNNGGC	2 cut(s)	63, 336
BglII	AGATCT	1 cut(s)	913
BlpI	GCTNAGC	1 cut(s)	1289
BmcAI	AGTACT	1 cut(s)	1172
Bme18I	GGWCC	2 cut(s)	936, 2548
BmeT110I	CYCGRG	3 cut(s)	239, 512, 2960
BmgBI	CACGTC	2 cut(s)	538, 1796
BmgT120I	GGNCC	5 cut(s)	936, 1055, 1056, 2450, 2548
BmiI	GGNNCC	8 cut(s)	154, 427, 1057, 1058, 1093, 1608, 2194, 2966
BmrI	ACTGGG	2 cut(s)	1371, 2625
BmsI	GCATC	3 cut(s)	1570, 2563, 2772
BmuI	ACTGGG	2 cut(s)	1371, 2625
BpiI	GAAGAC	5 cut(s)	864, 903, 1225, 1577, 2586
BplI	GAGNNNNNCTC	2 cut(s)	1170, 1202
BpmI	CTGGAG	5 cut(s)	1210, 1311, 2087, 2205, 3048
Bpu10I	CCTNAGC	3 cut(s)	784, 1563, 2367
Bpu1102I	GCTNAGC	1 cut(s)	1289
Bpu14I	TTCGAA	1 cut(s)	870
BpuEI	CTTGAG	1 cut(s)	3137
BpuMI	CCSGG	4 cut(s)	240, 241, 513, 514
BsaI	GGTCTC	3 cut(s)	128, 401, 3088
BsaXI	ACNNNNNCTCC	8 cut(s)	80, 110, 353, 383, 1824, 1854, 2456, 2486
Bsc4I	CCNNNNNNNGG	2 cut(s)	1127, 2999
Bse118I	RCCGGY	1 cut(s)	2447
Bse21I	CCTNAGG	1 cut(s)	1349
Bse3DI	GCAATG	5 cut(s)	1376, 1521, 2556, 2826, 3042
BseBI	CCWGG	6 cut(s)	984, 1096, 1122, 1248, 1716, 2017
BseGI	GGATG	4 cut(s)	1242, 1408, 2881, 2993
BseLI	CCNNNNNNNGG	2 cut(s)	1127, 2999
BseMI	GCAATG	5 cut(s)	1376, 1521, 2556, 2826, 3042
BseMII	CTCAG	5 cut(s)	1303, 1340, 1451, 1943, 2358
BseRI	GAGGAG	2 cut(s)	146, 419
BseSI	GKGCMC	5 cut(s)	155, 428, 1059, 2138, 2242
BsgI	GTGCAG	2 cut(s)	706, 1352
Bsh1236I	CGCG	8 cut(s)	60, 82, 206, 249, 333, 355, 479, 522
BshNI	GGYRCC	4 cut(s)	152, 425, 1606, 2192
BsiHKAI	GWGCWC	4 cut(s)	543, 2146, 2242, 2961
BsiHKCI	CYCGRG	3 cut(s)	239, 512, 2960
BsiSI	CCGG	4 cut(s)	240, 513, 2448, 2649
BslFI	GGGAC	3 cut(s)	545, 1767, 2290
BslI	CCNNNNNNNGG	2 cut(s)	1127, 2999
BsmAI	GTCTC	9 cut(s)	81, 128, 354, 401, 608, 972, 1499, 2476, 3088
BsmFI	GGGAC	3 cut(s)	545, 1767, 2290
Bso31I	GGTCTC	3 cut(s)	128, 401, 3088
BsoBI	CYCGRG	3 cut(s)	239, 512, 2960
Bsp119I	TTCGAA	1 cut(s)	870
Bsp120I	GGGCCC	1 cut(s)	1055
Bsp1286I	GDGCHC	8 cut(s)	155, 428, 543, 1059, 2138, 2146, 2242, 2961
Bsp1407I	TGTACA	2 cut(s)	763, 1239
Bsp1720I	GCTNAGC	1 cut(s)	1289
Bsp19I	CCATGG	3 cut(s)	1227, 1384, 2079
Bsp68I	TCGCGA	2 cut(s)	249, 522
BspCNI	CTCAG	5 cut(s)	1302, 1341, 1452, 1942, 2359
BspFNI	CGCG	8 cut(s)	60, 82, 206, 249, 333, 355, 479, 522
BspHI	TCATGA	1 cut(s)	1009
BspLI	GGNNCC	8 cut(s)	154, 427, 1057, 1058, 1093, 1608, 2194, 2966
BspMAI	CTGCAG	2 cut(s)	2365, 2980
BspMI	ACCTGC	2 cut(s)	200, 473
BspQI	GCTCTTC	1 cut(s)	730
BspT104I	TTCGAA	1 cut(s)	870
BspT107I	GGYRCC	4 cut(s)	152, 425, 1606, 2192
BspTI	CTTAAG	1 cut(s)	1253
BspTNI	GGTCTC	3 cut(s)	128, 401, 3088
BsrDI	GCAATG	5 cut(s)	1376, 1521, 2556, 2826, 3042
BsrFI	RCCGGY	1 cut(s)	2447
BsrGI	TGTACA	2 cut(s)	763, 1239
BssAI	RCCGGY	1 cut(s)	2447
Bst2UI	CCWGG	6 cut(s)	984, 1096, 1122, 1248, 1716, 2017
Bst6I	CTCTTC	8 cut(s)	224, 497, 730, 975, 1132, 2430, 2855, 2993
BstAFI	CTTAAG	1 cut(s)	1253
BstAPI	GCANNNNNTGC	5 cut(s)	192, 465, 720, 2813, 3041
BstAUI	TGTACA	2 cut(s)	763, 1239
BstBI	TTCGAA	1 cut(s)	870
BstDEI	CTNAG	9 cut(s)	784, 1264, 1289, 1349, 1460, 1537, 1563, 1929, 2367
BstDSI	CCRYGG	8 cut(s)	58, 80, 331, 353, 1227, 1384, 2047, 2079
BstF5I	GGATG	4 cut(s)	1242, 1408, 2881, 2993
BstFNI	CGCG	8 cut(s)	60, 82, 206, 249, 333, 355, 479, 522
BstHHI	GCGC	4 cut(s)	206, 479, 1383, 2461
BstMAI	GTCTC	9 cut(s)	81, 128, 354, 401, 608, 972, 1499, 2476, 3088
BstNI	CCWGG	6 cut(s)	984, 1096, 1122, 1248, 1716, 2017
BstSFI	CTRYAG	3 cut(s)	1174, 2361, 2976
BstSLI	GKGCMC	5 cut(s)	155, 428, 1059, 2138, 2242
BstUI	CGCG	8 cut(s)	60, 82, 206, 249, 333, 355, 479, 522
BstV2I	GAAGAC	5 cut(s)	864, 903, 1225, 1577, 2586
BstX2I	RGATCY	4 cut(s)	913, 1091, 2112, 2964
BstXI	CCANNNNNNTGG	3 cut(s)	145, 418, 1597
BstYI	RGATCY	4 cut(s)	913, 1091, 2112, 2964
Bsu36I	CCTNAGG	1 cut(s)	1349
BsuI	GTATCC	3 cut(s)	120, 393, 2061
BtgI	CCRYGG	8 cut(s)	58, 80, 331, 353, 1227, 1384, 2047, 2079
BtrI	CACGTC	2 cut(s)	538, 1796
BtsCI	GGATG	4 cut(s)	1242, 1408, 2881, 2993
BtuMI	TCGCGA	2 cut(s)	249, 522
BveI	ACCTGC	2 cut(s)	200, 473
CaiI	CAGNNNCTG	1 cut(s)	2597
CciI	TCATGA	1 cut(s)	1009
CfoI	GCGC	4 cut(s)	206, 479, 1383, 2461
Cfr10I	RCCGGY	1 cut(s)	2447
Cfr13I	GGNCC	5 cut(s)	936, 1055, 1056, 2450, 2548
Cfr42I	CCGCGG	4 cut(s)	61, 83, 334, 356
Cfr9I	CCCGGG	2 cut(s)	239, 512
Csp6I	GTAC	9 cut(s)	764, 1171, 1240, 1457, 1491, 1546, 1836, 1910, 2181
CspCI	CAANNNNNGTGG	4 cut(s)	28, 63, 301, 336
CviQI	GTAC	9 cut(s)	764, 1171, 1240, 1457, 1491, 1546, 1836, 1910, 2181
DdeI	CTNAG	9 cut(s)	784, 1264, 1289, 1349, 1460, 1537, 1563, 1929, 2367
DraIII	CACNNNGTG	1 cut(s)	2491
EaeI	YGGCCR	5 cut(s)	126, 399, 1935, 2391, 2646
Eam1104I	CTCTTC	8 cut(s)	224, 497, 730, 975, 1132, 2430, 2855, 2993
EarI	CTCTTC	8 cut(s)	224, 497, 730, 975, 1132, 2430, 2855, 2993
EciI	GGCGGA	2 cut(s)	187, 460
Eco147I	AGGCCT	3 cut(s)	254, 1347, 2151
Eco24I	GRGCYC	1 cut(s)	1059
Eco31I	GGTCTC	3 cut(s)	128, 401, 3088
Eco47I	GGWCC	2 cut(s)	936, 2548
Eco57I	CTGAAG	4 cut(s)	813, 1716, 2619, 2715
Eco81I	CCTNAGG	1 cut(s)	1349
Eco88I	CYCGRG	3 cut(s)	239, 512, 2960
EcoO109I	RGGNCCY	2 cut(s)	1055, 1056
EcoRII	CCWGG	6 cut(s)	982, 1094, 1120, 1246, 1714, 2015
EcoT38I	GRGCYC	1 cut(s)	1059
FalI	AAGNNNNNCTT	6 cut(s)	864, 896, 1247, 1279, 2852, 2884
FaqI	GGGAC	3 cut(s)	545, 1767, 2290
FauI	CCCGC	2 cut(s)	199, 472
FbaI	TGATCA	3 cut(s)	1995, 2211, 2692
FblI	GTMKAC	4 cut(s)	534, 999, 1322, 1449
FokI	GGATG	4 cut(s)	1229, 1415, 2888, 2980
FriOI	GRGCYC	1 cut(s)	1059
FspBI	CTAG	8 cut(s)	1509, 1550, 1634, 2147, 2153, 2588, 2624, 2657
GlaI	GCGC	4 cut(s)	205, 478, 1382, 2460
GsuI	CTGGAG	5 cut(s)	1210, 1311, 2087, 2205, 3048
HapII	CCGG	4 cut(s)	240, 513, 2448, 2649
HhaI	GCGC	4 cut(s)	206, 479, 1383, 2461
Hin6I	GCGC	4 cut(s)	204, 477, 1381, 2459
HinP1I	GCGC	4 cut(s)	204, 477, 1381, 2459
HincII	GTYRAC	5 cut(s)	535, 925, 1629, 2308, 2374
HindII	GTYRAC	5 cut(s)	535, 925, 1629, 2308, 2374
HpaI	GTTAAC	2 cut(s)	2308, 2374
HpaII	CCGG	4 cut(s)	240, 513, 2448, 2649
Hpy188I	TCNGA	8 cut(s)	114, 387, 1579, 1735, 1888, 1932, 1953, 2668
Hpy188III	TCNNGA	8 cut(s)	248, 521, 1010, 1189, 1634, 2320, 2339, 2962
Hpy99I	CGWCG	2 cut(s)	539, 2752
HpyCH4IV	ACGT	3 cut(s)	537, 677, 1795
HpyF3I	CTNAG	9 cut(s)	784, 1264, 1289, 1349, 1460, 1537, 1563, 1929, 2367
HpySE526I	ACGT	3 cut(s)	537, 677, 1795
HspAI	GCGC	4 cut(s)	204, 477, 1381, 2459
KroI	GCCGGC	1 cut(s)	2447
KroNI	GCCGGC	1 cut(s)	2449
Ksp22I	TGATCA	3 cut(s)	1995, 2211, 2692
KspAI	GTTAAC	2 cut(s)	2308, 2374
KspI	CCGCGG	4 cut(s)	61, 83, 334, 356
LguI	GCTCTTC	1 cut(s)	730
LmnI	GCTCC	7 cut(s)	200, 473, 1130, 1300, 1948, 3029, 3103
LweI	GCATC	3 cut(s)	1570, 2563, 2772
MaeI	CTAG	8 cut(s)	1509, 1550, 1634, 2147, 2153, 2588, 2624, 2657
MaeII	ACGT	3 cut(s)	537, 677, 1795
MaeIII	GTNAC	3 cut(s)	566, 2483, 2864
MfeI	CAATTG	3 cut(s)	2286, 2808, 2832
MflI	RGATCY	4 cut(s)	913, 1091, 2112, 2964
MhlI	GDGCHC	8 cut(s)	155, 428, 543, 1059, 2138, 2146, 2242, 2961
MlsI	TGGCCA	2 cut(s)	1937, 2393
MluNI	TGGCCA	2 cut(s)	1937, 2393
MlyI	GAGTC	8 cut(s)	167, 440, 1188, 1624, 1931, 2272, 2310, 2765
MmeI	TCCRAC	2 cut(s)	610, 1861
Mox20I	TGGCCA	2 cut(s)	1937, 2393
MroNI	GCCGGC	1 cut(s)	2447
MroXI	GAANNNNTTC	2 cut(s)	1024, 2909
MscI	TGGCCA	2 cut(s)	1937, 2393
MseI	TTAA	8 cut(s)	885, 918, 1220, 1254, 1409, 1485, 2307, 2373
MslI	CAYNNNNRTG	8 cut(s)	143, 185, 416, 458, 680, 1011, 1073, 1767
Msp20I	TGGCCA	2 cut(s)	1937, 2393
MspA1I	CMGCKG	6 cut(s)	60, 82, 333, 355, 841, 2366
MspCI	CTTAAG	1 cut(s)	1253
MspI	CCGG	4 cut(s)	240, 513, 2448, 2649
MunI	CAATTG	3 cut(s)	2286, 2808, 2832
MvaI	CCWGG	6 cut(s)	984, 1096, 1122, 1248, 1716, 2017
MvnI	CGCG	8 cut(s)	60, 82, 206, 249, 333, 355, 479, 522
NaeI	GCCGGC	1 cut(s)	2449
NciI	CCSGG	4 cut(s)	240, 241, 513, 514
NcoI	CCATGG	3 cut(s)	1227, 1384, 2079
NgoMIV	GCCGGC	1 cut(s)	2447
NlaIV	GGNNCC	8 cut(s)	154, 427, 1057, 1058, 1093, 1608, 2194, 2966
NmeAIII	GCCGAG	2 cut(s)	154, 427
NmuCI	GTSAC	1 cut(s)	2483
NruI	TCGCGA	2 cut(s)	249, 522
NspV	TTCGAA	1 cut(s)	870
OliI	CACNNNNGTG	3 cut(s)	143, 416, 680
PagI	TCATGA	1 cut(s)	1009
PasI	CCCWGGG	1 cut(s)	983
PceI	AGGCCT	3 cut(s)	254, 1347, 2151
PciSI	GCTCTTC	1 cut(s)	730
PdiI	GCCGGC	1 cut(s)	2449
PdmI	GAANNNNTTC	2 cut(s)	1024, 2909
PfeI	GAWTC	7 cut(s)	244, 517, 737, 867, 1315, 2440, 2620
PflFI	GACNNNGTC	1 cut(s)	2468
PflMI	CCANNNNNTGG	2 cut(s)	1127, 2999
PleI	GAGTC	8 cut(s)	166, 439, 1187, 1624, 1930, 2272, 2310, 2765
PpsI	GAGTC	8 cut(s)	166, 439, 1187, 1624, 1930, 2272, 2310, 2765
PshBI	ATTAAT	1 cut(s)	885
PsiI	TTATAA	1 cut(s)	1307
Psp6I	CCWGG	6 cut(s)	982, 1094, 1120, 1246, 1714, 2015
PspGI	CCWGG	6 cut(s)	982, 1094, 1120, 1246, 1714, 2015
PspN4I	GGNNCC	8 cut(s)	154, 427, 1057, 1058, 1093, 1608, 2194, 2966
PspOMI	GGGCCC	1 cut(s)	1055
PspPI	GGNCC	5 cut(s)	936, 1055, 1056, 2450, 2548
PstI	CTGCAG	2 cut(s)	2365, 2980
PstNI	CAGNNNCTG	1 cut(s)	2597
PsuI	RGATCY	4 cut(s)	913, 1091, 2112, 2964
PsyI	GACNNNGTC	1 cut(s)	2468
PvuII	CAGCTG	2 cut(s)	841, 2366
RruI	TCGCGA	2 cut(s)	249, 522
RsaI	GTAC	9 cut(s)	765, 1172, 1241, 1458, 1492, 1547, 1837, 1911, 2182
RsaNI	GTAC	9 cut(s)	764, 1171, 1240, 1457, 1491, 1546, 1836, 1910, 2181
RseI	CAYNNNNRTG	8 cut(s)	143, 185, 416, 458, 680, 1011, 1073, 1767
SacII	CCGCGG	4 cut(s)	61, 83, 334, 356
SalI	GTCGAC	1 cut(s)	533
SapI	GCTCTTC	1 cut(s)	730
SaqAI	TTAA	8 cut(s)	885, 918, 1220, 1254, 1409, 1485, 2307, 2373
Sau96I	GGNCC	5 cut(s)	936, 1055, 1056, 2450, 2548
ScaI	AGTACT	1 cut(s)	1172
SchI	GAGTC	8 cut(s)	167, 440, 1188, 1624, 1931, 2272, 2310, 2765
SduI	GDGCHC	8 cut(s)	155, 428, 543, 1059, 2138, 2146, 2242, 2961
SfaNI	GCATC	3 cut(s)	1570, 2563, 2772
SfcI	CTRYAG	3 cut(s)	1174, 2361, 2976
Sfr303I	CCGCGG	4 cut(s)	61, 83, 334, 356
SfuI	TTCGAA	1 cut(s)	870
SgrBI	CCGCGG	4 cut(s)	61, 83, 334, 356
SinI	GGWCC	2 cut(s)	936, 2548
SmaI	CCCGGG	2 cut(s)	241, 514
SmiMI	CAYNNNNRTG	8 cut(s)	143, 185, 416, 458, 680, 1011, 1073, 1767
SmlI	CTYRAG	2 cut(s)	1253, 3116
SmoI	CTYRAG	2 cut(s)	1253, 3116
SpeI	ACTAGT	1 cut(s)	1508
SseBI	AGGCCT	3 cut(s)	254, 1347, 2151
SspMI	CTAG	8 cut(s)	1509, 1550, 1634, 2147, 2153, 2588, 2624, 2657
StuI	AGGCCT	3 cut(s)	254, 1347, 2151
TaiI	ACGT	3 cut(s)	540, 680, 1798
TaqI	TCGA	6 cut(s)	534, 870, 2265, 2338, 2571, 3138
TaqII	GACCGA	1 cut(s)	953
TatI	WGTACW	3 cut(s)	763, 1170, 1239
TauI	GCSGC	8 cut(s)	60, 63, 66, 85, 333, 336, 339, 358
TfiI	GAWTC	7 cut(s)	244, 517, 737, 867, 1315, 2440, 2620
Tru1I	TTAA	8 cut(s)	885, 918, 1220, 1254, 1409, 1485, 2307, 2373
Tru9I	TTAA	8 cut(s)	885, 918, 1220, 1254, 1409, 1485, 2307, 2373
TseFI	GTSAC	1 cut(s)	2483
Tsp45I	GTSAC	1 cut(s)	2483
TspGWI	ACGGA	4 cut(s)	166, 439, 599, 1444
TspMI	CCCGGG	2 cut(s)	239, 512
Tth111I	GACNNNGTC	1 cut(s)	2468
Van91I	CCANNNNNTGG	2 cut(s)	1127, 2999
Vha464I	CTTAAG	1 cut(s)	1253
VneI	GTGCAC	1 cut(s)	2238
VpaK11BI	GGWCC	2 cut(s)	936, 2548
VspI	ATTAAT	1 cut(s)	885
XapI	RAATTY	7 cut(s)	1023, 1063, 1113, 1161, 1976, 2042, 2420
XbaI	TCTAGA	1 cut(s)	1633
XcmI	CCANNNNNNNNNTGG	2 cut(s)	145, 418
XmaI	CCCGGG	2 cut(s)	239, 512
XmiI	GTMKAC	4 cut(s)	534, 999, 1322, 1449
XmnI	GAANNNNTTC	2 cut(s)	1024, 2909
XspI	CTAG	8 cut(s)	1509, 1550, 1634, 2147, 2153, 2588, 2624, 2657
ZrmI	AGTACT	1 cut(s)	1172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.