Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000221.1

Orthogroup ID

OG0002025

Physical Location & Seq

Genomic Coordinates Forward (+)

146197 .. 152640

Gene Structure 6444 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

atgtcacccgggccacggccacactacccctcgctgccccgatacccgaaaggcacgtctccagaagcaacgaggaaggccaggggttctgaggtgaaggccttgttctctgtggttggaatgacacgtgctgcctgtgtcgggtctgtggagaaggcggtgaagcggctcccggggatacaggaggccgccgtggacgtgttgaataacacagcccttgttctctactatcccagcttcgtcaatgaggacaaaatatgtgaggctattgaagatgctggatttgaagctaaactgattaagttggagatgaatgacaaaaccagacaagtatgcagaataagcataagtggaatgacttgcacgtcttgctcctccactgttgaatcagccctgcaagcaattcatggagtgcaaggagctcaggtggccttagcaactgaagaagcagagattcattatgacccaagcattgttacctcccatcagctattggaaaccatagaaatcaccggatttgaagccaaacttattagtttaggggaaggcattagcaagatagagcttcaagttgatggcataaaggttggacagtcgattagagcaatagcaaaaactcttgaagcacttcctggggttcaagacatagagacatttcctgaactcaacaagatttctatttcttataaaccagagatgataggaccaagaaccctcatcgaagccatcgaatcatcagggtccgcgcatttcaaagcaatgatatacccggaaggagaagaagacactcatagacagaaggaaattaagcaatactataaattcttcttatggagtttgttttgtacagttcctgtgtttttaacctccatggtcttgatgtatattcctggaattaagaaggtgttggatgtcaaaattgtgaataagttgagtgttggtcagattctgaggtgggagttgtccactccagtgcagttcatcataggcaagaggttctacattggagcatacaaagcattgcgccatggttcagctaacatggatgttttgattgccttgggaacaaatgcagcctacttctactcggtctacatagttttaagatctgcaacccataaggatttcaagggtacagatttttttgagactagttcaatgcttattacattcatcctgttaggaaagtatctagaggtcttggcaaaaggcaagacatctgaggctattgccaagctaatggacttggcaccagaaaaggcaacactgctaactttgggtgaggaaggaaatgtgataaatgaacaagaaatggatagccggttgatacaaaagaacgatctgattaaagttatgcctggtgccaaagtagcttgtgatggtgttgttatgtggggacaaagtcatgtgaatgaaagcatgataactggagaagcacggccagtgacaaaaaagaaggatgatgcagttattggaggcaccgtgaatgagaatggagtgttgcatattaaggcaacaagagttgggtcagagagtgctctttcacagattgtaaggcttgtcgagtctgcccagatggctaaagctcctgtacaaaagtttgctgatcgcatttccaaatactttgtgccagtggtcatcacactttcattttcgacttggcttgcctggttcttaggagggaagttccacaggtacccacgttcttggataccatcttcaatcgatagttttgagcttgctcttcagtttgggatctcggtcatggtcattgcctgcccttgtgctttaggcctagcaactcctacagctgttatggttggtacaggagttggtgcatctcaaggtatactgatcaaaggcggacatgcattagagagtgcgcaaaaggtgaactgcattgtgtttgacaagacagggactctcacagtagggaagccagtggttgttagcaccaaaatctttaacaaaatgctacctcaagaattctatgaactagttgctgcaactgaggtcaacagtgagcacccactagccaaagctattgtggaatatgccaagaaaatcagagaagatgaagagaattctacctggccagaagcacaaaacttcgcatcccttaccggccaaggagtgaaggcagttgttcgaaataaggaaataactgtaggcaacaagagtttaatgttggacattaacattgcgattccagaggaggctgaagttgcacttgcacaaactgaagcattggctcaaactgggattttaatagctgtagataacgaaatagcaggacttatagccatatctgatccactgaaacctggtgctcgagaagtcatttccatacttaaatctatgagagttagaagcatcatggttacaggtgacaattggggtacagccaattctattgccaaggaaactgaaattgagacggtgattgcagaagccaaaccccagcacaaagctgagaaaataaaagatttgcaggctgaaggctacactgtggcaatggtgggagatgggatcaatgactcaccggcacttgtggcagcagatgttggaatggcaattggtgctggcacagacattgcgatcgaggcagctgacattgttcttatgaagagcaacttggaagaggttataacagccattgacctttcccggaaaaccttttcccgtattcgtctgaactacatttgggcattgggttacaatgtgcttggaatcccgatagctgcaggggtcctttttccttccactaggttcagattaccaccatggattgcaggagctgcaatggcagcatcctccgttagtgttgtatgctgttctctgttgttgaagaactacaagagacccaagaagttggatatccttgagatacatgaagtaagggttgagtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0008150 GO:0008324 GO:0009636 GO:0010035 GO:0010038 GO:0010273 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046688 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061687 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0097501 GO:0098655 GO:0098660 GO:0098662 GO:0098754 GO:0099131 GO:0099132 GO:1990169

KEGG Pathways

Metabolic & Signaling

ko01524 ko04016 map01524 map04016

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

969

Amino Acids

105.29

Weight (kDa)

6.49

Isoelectric Point (pI)

38.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	36 - 96	2.2e-11	Heavy-metal-associated domain
HMA	PF00403	114 - 174	1.2e-12	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	416 - 522	9.9e-26	P-type ATPase actuator domain
Hydrolase	PF00702	632 - 854	1.8e-33	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63440
fragaria_vesca	FvH4_5g02090 FvH4_5g02090 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091 FvH4_5g02091
malus_domestica	MD06G1080800.v1.1 MD06G1080900.v1.1 MD14G1102600.v1.1
prunus_persica	Prupe.5G097900_v2.0.a1 Prupe.5G097900_v2.0.a1 Prupe.5G098000_v2.0.a1
pyrus_communis	pycom06g07780 pycom06g07790 pycom14g09890
rosa_chinensis	RchiOBHm_Chr7g0197781 RchiOBHm_Chr7g0198111 RchiOBHm_Chr7g0198141
rosa_laevigata	RLG00000003885 RLG00000003888 RLG00000019170
rosa_multiflora	Rmu_sc0000221.1_g000018 Rmu_sc0000221.1_g000030 Rmu_sc0006732.1_g000016
rosa_roxburghii	Rroxscaffold_3G00257940 Rroxscaffold_3G00257970 Rroxscaffold_3G00257990
rosa_rugosa	Rorug07G0045700 Rorug07G0045700 Rorug07G0045800 Rorug07G0045800 Rorug07G0045900
rosa_samantha	Rh7AG169100 Rh7AG169200 Rh7BG169900 Rh7BG172300 Rh7CG178600 Rh7CG178700 Rh7DG169200 Rh7DG171400 Rh7DG171800
rosa_wichuraiana	Rw7G014570 Rw7G014780 Rw7G014790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	687, 2648
AasI	GACNNNNNNGTC	1 cut(s)	364
Acc16I	TGCGCA	1 cut(s)	1884
Acc65I	GGTACC	1 cut(s)	1695
AccB1I	GGYRCC	4 cut(s)	1249, 1361, 1478, 1695
AccI	GTMKAC	2 cut(s)	1092, 1849
AccII	CGCG	1 cut(s)	746
AciI	CCGC	5 cut(s)	158, 166, 189, 744, 1863
AclWI	GGATC	3 cut(s)	1763, 2306, 2537
AcoI	YGGCCR	4 cut(s)	17, 1439, 2093, 2125
AcsI	RAATTY	3 cut(s)	821, 1985, 2083
AcuI	CTGAAG	5 cut(s)	462, 1730, 2241, 2262, 2517
AcvI	CACGTG	1 cut(s)	128
AfaI	GTAC	6 cut(s)	847, 1135, 1593, 1697, 1825, 2401
AfiI	CCNNNNNNNGG	2 cut(s)	15, 141
AflIII	ACRYGT	2 cut(s)	125, 198
AhlI	ACTAGT	2 cut(s)	1151, 1996
AjiI	CACGTC	3 cut(s)	57, 199, 366
AjnI	CCWGG	7 cut(s)	80, 631, 889, 1357, 1667, 2090, 2323
AjuI	GAANNNNNNNTTGG	2 cut(s)	2618, 2650
AleI	CACNNNNGTG	1 cut(s)	971
Alw21I	GWGCWC	4 cut(s)	424, 1540, 2028, 2332
Alw26I	GTCTC	5 cut(s)	63, 644, 1142, 2429, 2854
AlwI	GGATC	3 cut(s)	1763, 2306, 2537
AlwNI	CAGNNNCTG	3 cut(s)	854, 949, 2798
Ama87I	CYCGRG	3 cut(s)	8, 172, 2331
ApeKI	GCWGC	9 cut(s)	34, 131, 1073, 2003, 2555, 2606, 2741, 2798, 2807
ApoI	RAATTY	3 cut(s)	821, 1985, 2083
ArsI	GACNNNNNNTTYG	2 cut(s)	41, 73
Asp700I	GAANNNNTTC	1 cut(s)	627
Asp718I	GGTACC	1 cut(s)	1695
AspLEI	GCGC	3 cut(s)	748, 1026, 1885
AspS9I	GGNCC	4 cut(s)	11, 704, 741, 2749
AsuC2I	CCSGG	6 cut(s)	9, 10, 173, 174, 770, 2668
AsuHPI	GGTGA	8 cut(s)	106, 172, 502, 1292, 1903, 2399, 2452, 2532
AsuII	TTCGAA	1 cut(s)	2149
AvaI	CYCGRG	3 cut(s)	8, 172, 2331
AvaII	GGWCC	3 cut(s)	704, 741, 2749
BaeI	ACNNNNGTAYC	4 cut(s)	1687, 1720, 1815, 1848
BalI	TGGCCA	1 cut(s)	2095
BanI	GGYRCC	4 cut(s)	1249, 1361, 1478, 1695
BanII	GRGCYC	1 cut(s)	424
BbrPI	CACGTG	1 cut(s)	128
BbsI	GAAGAC	1 cut(s)	789
Bbv12I	GWGCWC	4 cut(s)	424, 1540, 2028, 2332
BbvI	GCAGC	9 cut(s)	21, 118, 1085, 1990, 2567, 2618, 2728, 2785, 2819
BccI	CCATC	7 cut(s)	492, 569, 734, 1373, 1570, 1723, 2519
BceAI	ACGGC	3 cut(s)	32, 176, 1454
BciT130I	CCWGG	7 cut(s)	82, 633, 891, 1359, 1669, 2092, 2325
BciVI	GTATCC	2 cut(s)	171, 1704
BclI	TGATCA	1 cut(s)	1854
BcnI	CCSGG	6 cut(s)	9, 10, 173, 174, 770, 2668
BcoDI	GTCTC	5 cut(s)	63, 644, 1142, 2429, 2854
BcuI	ACTAGT	2 cut(s)	1151, 1996
BfaI	CTAG	6 cut(s)	1152, 1193, 1796, 1997, 2033, 2766
BfmI	CTRYAG	4 cut(s)	1806, 2166, 2274, 2742
BfuI	GTATCC	2 cut(s)	171, 1704
BglI	GCCNNNNNGGC	1 cut(s)	1577
BglII	AGATCT	1 cut(s)	1106
Bme18I	GGWCC	3 cut(s)	704, 741, 2749
BmeT110I	CYCGRG	3 cut(s)	8, 172, 2331
BmgBI	CACGTC	3 cut(s)	57, 199, 366
BmgT120I	GGNCC	4 cut(s)	11, 704, 741, 2749
BmiI	GGNNCC	7 cut(s)	170, 742, 1251, 1363, 1480, 1697, 2750
BmrI	ACTGGG	1 cut(s)	2268
BmsI	GCATC	6 cut(s)	265, 1453, 1847, 2123, 2382, 2819
BmuI	ACTGGG	1 cut(s)	2268
BpiI	GAAGAC	1 cut(s)	789
BpmI	CTGGAG	3 cut(s)	45, 954, 1449
Bpu10I	CCTNAGC	2 cut(s)	423, 433
Bpu14I	TTCGAA	1 cut(s)	2149
BpuEI	CTTGAG	3 cut(s)	1827, 1965, 2903
BpuMI	CCSGG	6 cut(s)	9, 10, 173, 174, 770, 2668
Bsa29I	ATCGAT	1 cut(s)	1725
BsaAI	YACGTR	1 cut(s)	128
BsaI	GGTCTC	1 cut(s)	2854
BsaWI	WCCGGW	1 cut(s)	512
BsaXI	ACNNNNNCTCC	4 cut(s)	411, 441, 1467, 1497
Bsc4I	CCNNNNNNNGG	2 cut(s)	15, 141
Bse118I	RCCGGY	3 cut(s)	1320, 2123, 2542
Bse1I	ACTGG	6 cut(s)	971, 1432, 1442, 1631, 1940, 2263
Bse3DI	GCAATG	7 cut(s)	765, 1019, 1770, 2199, 2520, 2592, 2808
BseBI	CCWGG	7 cut(s)	82, 633, 891, 1359, 1669, 2092, 2325
BseCI	ATCGAT	1 cut(s)	1725
BseGI	GGATG	6 cut(s)	916, 1051, 1173, 1465, 2114, 2810
BseLI	CCNNNNNNNGG	2 cut(s)	15, 141
BseMI	GCAATG	7 cut(s)	765, 1019, 1770, 2199, 2520, 2592, 2808
BseMII	CTCAG	6 cut(s)	81, 437, 941, 1212, 2001, 2463
BseNI	ACTGG	6 cut(s)	971, 1432, 1442, 1631, 1940, 2263
BseRI	GAGGAG	2 cut(s)	364, 2228
BseXI	GCAGC	9 cut(s)	21, 118, 1085, 1990, 2567, 2618, 2728, 2785, 2819
BseYI	CCCAGC	2 cut(s)	233, 2460
BsgI	GTGCAG	1 cut(s)	995
Bsh1236I	CGCG	1 cut(s)	746
Bsh1285I	CGRYCG	1 cut(s)	2601
BshNI	GGYRCC	4 cut(s)	1249, 1361, 1478, 1695
BshVI	ATCGAT	1 cut(s)	1725
BsiEI	CGRYCG	1 cut(s)	2601
BsiHKAI	GWGCWC	4 cut(s)	424, 1540, 2028, 2332
BsiHKCI	CYCGRG	3 cut(s)	8, 172, 2331
BsiSI	CCGG	8 cut(s)	9, 173, 513, 770, 1321, 2124, 2543, 2668
BslFI	GGGAC	2 cut(s)	1410, 1933
BslI	CCNNNNNNNGG	2 cut(s)	15, 141
BsmAI	GTCTC	5 cut(s)	63, 644, 1142, 2429, 2854
BsmBI	CGTCTC	2 cut(s)	63, 2429
BsmFI	GGGAC	2 cut(s)	1410, 1933
Bso31I	GGTCTC	1 cut(s)	2854
BsoBI	CYCGRG	3 cut(s)	8, 172, 2331
Bsp119I	TTCGAA	1 cut(s)	2149
Bsp1286I	GDGCHC	4 cut(s)	424, 1540, 2028, 2332
Bsp1407I	TGTACA	2 cut(s)	845, 1591
Bsp143I	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
Bsp19I	CCATGG	3 cut(s)	870, 1027, 2783
BspACI	CCGC	5 cut(s)	158, 166, 189, 744, 1863
BspCNI	CTCAG	6 cut(s)	82, 436, 942, 1213, 2002, 2464
BspDI	ATCGAT	1 cut(s)	1725
BspFNI	CGCG	1 cut(s)	746
BspLI	GGNNCC	7 cut(s)	170, 742, 1251, 1363, 1480, 1697, 2750
BspMAI	CTGCAG	1 cut(s)	2746
BspPI	GGATC	3 cut(s)	1763, 2306, 2537
BspQI	GCTCTTC	2 cut(s)	1749, 2621
BspT104I	TTCGAA	1 cut(s)	2149
BspT107I	GGYRCC	4 cut(s)	1249, 1361, 1478, 1695
BspTNI	GGTCTC	1 cut(s)	2854
BsrDI	GCAATG	7 cut(s)	765, 1019, 1770, 2199, 2520, 2592, 2808
BsrFI	RCCGGY	3 cut(s)	1320, 2123, 2542
BsrGI	TGTACA	2 cut(s)	845, 1591
BsrI	ACTGG	6 cut(s)	971, 1432, 1442, 1631, 1940, 2263
BssAI	RCCGGY	3 cut(s)	1320, 2123, 2542
BssMI	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
BssNAI	GTATAC	1 cut(s)	1850
BssT1I	CCWWGG	6 cut(s)	870, 1027, 1059, 2128, 2418, 2783
Bst1107I	GTATAC	1 cut(s)	1850
Bst2UI	CCWGG	7 cut(s)	82, 633, 891, 1359, 1669, 2092, 2325
Bst4CI	ACNGT	9 cut(s)	382, 594, 850, 1483, 1930, 2021, 2167, 2440, 2509
Bst6I	CTCTTC	4 cut(s)	1749, 2073, 2621, 2634
BstAPI	GCANNNNNTGC	3 cut(s)	369, 2579, 2798
BstAUI	TGTACA	2 cut(s)	845, 1591
BstBAI	YACGTR	1 cut(s)	128
BstBI	TTCGAA	1 cut(s)	2149
BstC8I	GCNNGC	6 cut(s)	399, 1665, 1740, 1777, 2493, 2584
BstDEI	CTNAG	8 cut(s)	90, 423, 433, 950, 1221, 1675, 2010, 2472
BstDSI	CCRYGG	5 cut(s)	14, 192, 870, 1027, 2783
BstF5I	GGATG	6 cut(s)	916, 1051, 1173, 1465, 2114, 2810
BstFNI	CGCG	1 cut(s)	746
BstHHI	GCGC	3 cut(s)	748, 1026, 1885
BstKTI	GATC	8 cut(s)	1109, 1342, 1609, 1758, 1857, 2314, 2532, 2601
BstMAI	GTCTC	5 cut(s)	63, 644, 1142, 2429, 2854
BstMBI	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
BstMCI	CGRYCG	1 cut(s)	2601
BstNI	CCWGG	7 cut(s)	82, 633, 891, 1359, 1669, 2092, 2325
BstNSI	RCATGY	1 cut(s)	1871
BstSFI	CTRYAG	4 cut(s)	1806, 2166, 2274, 2742
BstUI	CGCG	1 cut(s)	746
BstV1I	GCAGC	9 cut(s)	21, 118, 1085, 1990, 2567, 2618, 2728, 2785, 2819
BstV2I	GAAGAC	1 cut(s)	789
BstX2I	RGATCY	2 cut(s)	1106, 1755
BstXI	CCANNNNNNTGG	3 cut(s)	1240, 1707, 2872
BstYI	RGATCY	2 cut(s)	1106, 1755
BstZ17I	GTATAC	1 cut(s)	1850
Bsu15I	ATCGAT	1 cut(s)	1725
BsuI	GTATCC	2 cut(s)	171, 1704
BsuTUI	ATCGAT	1 cut(s)	1725
BtgI	CCRYGG	5 cut(s)	14, 192, 870, 1027, 2783
BtrI	CACGTC	3 cut(s)	57, 199, 366
BtsCI	GGATG	6 cut(s)	916, 1051, 1173, 1465, 2114, 2810
BtsI	GCAGTG	1 cut(s)	1265
BtsIMutI	CAGTG	9 cut(s)	378, 978, 1265, 1449, 1638, 1947, 2026, 2315, 2505
Cac8I	GCNNGC	6 cut(s)	399, 1665, 1740, 1777, 2493, 2584
CaiI	CAGNNNCTG	3 cut(s)	854, 949, 2798
CfoI	GCGC	3 cut(s)	748, 1026, 1885
Cfr10I	RCCGGY	3 cut(s)	1320, 2123, 2542
Cfr13I	GGNCC	4 cut(s)	11, 704, 741, 2749
Cfr9I	CCCGGG	2 cut(s)	8, 172
ClaI	ATCGAT	1 cut(s)	1725
CsiI	ACCWGGT	1 cut(s)	2323
Csp6I	GTAC	6 cut(s)	846, 1134, 1592, 1696, 1824, 2400
CviQI	GTAC	6 cut(s)	846, 1134, 1592, 1696, 1824, 2400
DdeI	CTNAG	8 cut(s)	90, 423, 433, 950, 1221, 1675, 2010, 2472
DpnI	GATC	8 cut(s)	1108, 1341, 1608, 1757, 1856, 2313, 2531, 2600
DpnII	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
DrdI	GACNNNNNNGTC	1 cut(s)	364
DseDI	GACNNNNNNGTC	1 cut(s)	364
EaeI	YGGCCR	4 cut(s)	17, 1439, 2093, 2125
Eam1104I	CTCTTC	4 cut(s)	1749, 2073, 2621, 2634
EarI	CTCTTC	4 cut(s)	1749, 2073, 2621, 2634
EciI	GGCGGA	1 cut(s)	1878
Ecl136II	GAGCTC	1 cut(s)	422
Eco130I	CCWWGG	6 cut(s)	870, 1027, 1059, 2128, 2418, 2783
Eco147I	AGGCCT	2 cut(s)	101, 1794
Eco24I	GRGCYC	1 cut(s)	424
Eco31I	GGTCTC	1 cut(s)	2854
Eco32I	GATATC	1 cut(s)	2878
Eco47I	GGWCC	3 cut(s)	704, 741, 2749
Eco53kI	GAGCTC	1 cut(s)	422
Eco57I	CTGAAG	5 cut(s)	462, 1730, 2241, 2262, 2517
Eco72I	CACGTG	1 cut(s)	128
Eco88I	CYCGRG	3 cut(s)	8, 172, 2331
EcoICRI	GAGCTC	1 cut(s)	422
EcoO109I	RGGNCCY	1 cut(s)	2749
EcoRI	GAATTC	2 cut(s)	1985, 2083
EcoRII	CCWGG	7 cut(s)	80, 631, 889, 1357, 1667, 2090, 2323
EcoRV	GATATC	1 cut(s)	2878
EcoT14I	CCWWGG	6 cut(s)	870, 1027, 1059, 2128, 2418, 2783
EcoT22I	ATGCAT	1 cut(s)	1873
EcoT38I	GRGCYC	1 cut(s)	424
ErhI	CCWWGG	6 cut(s)	870, 1027, 1059, 2128, 2418, 2783
Esp3I	CGTCTC	2 cut(s)	63, 2429
FalI	AAGNNNNNCTT	6 cut(s)	513, 545, 2214, 2246, 2618, 2650
FaqI	GGGAC	2 cut(s)	1410, 1933
FbaI	TGATCA	1 cut(s)	1854
FblI	GTMKAC	2 cut(s)	1092, 1849
FokI	GGATG	6 cut(s)	923, 1058, 1160, 1472, 2101, 2797
FriOI	GRGCYC	1 cut(s)	424
FspBI	CTAG	6 cut(s)	1152, 1193, 1796, 1997, 2033, 2766
FspI	TGCGCA	1 cut(s)	1884
GlaI	GCGC	3 cut(s)	747, 1025, 1884
GsaI	CCCAGC	2 cut(s)	237, 2464
GsuI	CTGGAG	3 cut(s)	45, 954, 1449
HapII	CCGG	8 cut(s)	9, 173, 513, 770, 1321, 2124, 2543, 2668
HhaI	GCGC	3 cut(s)	748, 1026, 1885
Hin6I	GCGC	3 cut(s)	746, 1024, 1883
HinP1I	GCGC	3 cut(s)	746, 1024, 1883
HincII	GTYRAC	1 cut(s)	2017
HindII	GTYRAC	1 cut(s)	2017
HinfI	GANTC	9 cut(s)	386, 454, 731, 946, 1565, 1921, 2206, 2537, 2730
HpaII	CCGG	8 cut(s)	9, 173, 513, 770, 1321, 2124, 2543, 2668
HphI	GGTGA	8 cut(s)	106, 172, 502, 1292, 1903, 2399, 2452, 2532
Hpy166II	GTNNAC	6 cut(s)	196, 966, 1093, 1850, 1894, 2017
Hpy8I	GTNNAC	6 cut(s)	196, 966, 1093, 1850, 1894, 2017
HpyCH4III	ACNGT	9 cut(s)	382, 594, 850, 1483, 1930, 2021, 2167, 2440, 2509
HpyCH4IV	ACGT	5 cut(s)	56, 127, 198, 365, 1702
HpyF3I	CTNAG	8 cut(s)	90, 423, 433, 950, 1221, 1675, 2010, 2472
HpySE526I	ACGT	5 cut(s)	56, 127, 198, 365, 1702
HspAI	GCGC	3 cut(s)	746, 1024, 1883
KpnI	GGTACC	1 cut(s)	1699
Ksp22I	TGATCA	1 cut(s)	1854
Kzo9I	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
LguI	GCTCTTC	2 cut(s)	1749, 2621
LmnI	GCTCC	6 cut(s)	174, 377, 419, 1007, 1591, 2795
Lsp1109I	GCAGC	9 cut(s)	21, 118, 1085, 1990, 2567, 2618, 2728, 2785, 2819
LweI	GCATC	6 cut(s)	265, 1453, 1847, 2123, 2382, 2819
MabI	ACCWGGT	1 cut(s)	2323
MaeI	CTAG	6 cut(s)	1152, 1193, 1796, 1997, 2033, 2766
MaeII	ACGT	5 cut(s)	56, 127, 198, 365, 1702
MaeIII	GTNAC	6 cut(s)	3, 475, 1444, 2380, 2387, 2714
MalI	GATC	8 cut(s)	1108, 1341, 1608, 1757, 1856, 2313, 2531, 2600
MboI	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
MfeI	CAATTG	2 cut(s)	2392, 2574
MflI	RGATCY	2 cut(s)	1106, 1755
MhlI	GDGCHC	4 cut(s)	424, 1540, 2028, 2332
MlsI	TGGCCA	1 cut(s)	2095
MluNI	TGGCCA	1 cut(s)	2095
MlyI	GAGTC	3 cut(s)	1574, 1915, 2531
MmeI	TCCRAC	7 cut(s)	97, 285, 568, 888, 2169, 2545, 2853
Mox20I	TGGCCA	1 cut(s)	2095
Mph1103I	ATGCAT	1 cut(s)	1873
MroXI	GAANNNNTTC	1 cut(s)	627
MscI	TGGCCA	1 cut(s)	2095
MslI	CAYNNNNRTG	2 cut(s)	971, 1410
Msp20I	TGGCCA	1 cut(s)	2095
MspA1I	CMGCKG	2 cut(s)	1811, 2609
MspI	CCGG	8 cut(s)	9, 173, 513, 770, 1321, 2124, 2543, 2668
MunI	CAATTG	2 cut(s)	2392, 2574
MvaI	CCWGG	7 cut(s)	82, 633, 891, 1359, 1669, 2092, 2325
MvnI	CGCG	1 cut(s)	746
NciI	CCSGG	6 cut(s)	9, 10, 173, 174, 770, 2668
NcoI	CCATGG	3 cut(s)	870, 1027, 2783
NdeII	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
NlaIV	GGNNCC	7 cut(s)	170, 742, 1251, 1363, 1480, 1697, 2750
NmuCI	GTSAC	3 cut(s)	3, 1444, 2387
NsbI	TGCGCA	1 cut(s)	1884
NsiI	ATGCAT	1 cut(s)	1873
NspI	RCATGY	1 cut(s)	1871
NspV	TTCGAA	1 cut(s)	2149
OliI	CACNNNNGTG	1 cut(s)	971
PaeR7I	CTCGAG	1 cut(s)	2331
PceI	AGGCCT	2 cut(s)	101, 1794
PciSI	GCTCTTC	2 cut(s)	1749, 2621
PcsI	WCGNNNNNNNCGW	1 cut(s)	726
PdmI	GAANNNNTTC	1 cut(s)	627
PfeI	GAWTC	6 cut(s)	386, 454, 731, 946, 2206, 2730
PflFI	GACNNNGTC	1 cut(s)	1401
PfoI	TCCNGGA	2 cut(s)	889, 2666
Ple19I	CGATCG	1 cut(s)	2601
PleI	GAGTC	3 cut(s)	1573, 1915, 2531
PmaCI	CACGTG	1 cut(s)	128
PmlI	CACGTG	1 cut(s)	128
PpsI	GAGTC	3 cut(s)	1573, 1915, 2531
Ppu21I	YACGTR	1 cut(s)	128
PpuMI	RGGWCCY	1 cut(s)	2749
PsiI	TTATAA	2 cut(s)	687, 2648
Psp124BI	GAGCTC	1 cut(s)	424
Psp5II	RGGWCCY	1 cut(s)	2749
Psp6I	CCWGG	7 cut(s)	80, 631, 889, 1357, 1667, 2090, 2323
PspCI	CACGTG	1 cut(s)	128
PspFI	CCCAGC	2 cut(s)	233, 2460
PspGI	CCWGG	7 cut(s)	80, 631, 889, 1357, 1667, 2090, 2323
PspN4I	GGNNCC	7 cut(s)	170, 742, 1251, 1363, 1480, 1697, 2750
PspPI	GGNCC	4 cut(s)	11, 704, 741, 2749
PspPPI	RGGWCCY	1 cut(s)	2749
PstI	CTGCAG	1 cut(s)	2746
PstNI	CAGNNNCTG	3 cut(s)	854, 949, 2798
PsuI	RGATCY	2 cut(s)	1106, 1755
PsyI	GACNNNGTC	1 cut(s)	1401
PvuI	CGATCG	1 cut(s)	2601
PvuII	CAGCTG	2 cut(s)	1811, 2609
RsaI	GTAC	6 cut(s)	847, 1135, 1593, 1697, 1825, 2401
RsaNI	GTAC	6 cut(s)	846, 1134, 1592, 1696, 1824, 2400
RseI	CAYNNNNRTG	2 cut(s)	971, 1410
SacI	GAGCTC	1 cut(s)	424
SapI	GCTCTTC	2 cut(s)	1749, 2621
Sau3AI	GATC	8 cut(s)	1106, 1339, 1606, 1755, 1854, 2311, 2529, 2598
Sau96I	GGNCC	4 cut(s)	11, 704, 741, 2749
SchI	GAGTC	3 cut(s)	1574, 1915, 2531
SduI	GDGCHC	4 cut(s)	424, 1540, 2028, 2332
SexAI	ACCWGGT	1 cut(s)	2323
SfaNI	GCATC	6 cut(s)	265, 1453, 1847, 2123, 2382, 2819
SfcI	CTRYAG	4 cut(s)	1806, 2166, 2274, 2742
Sfr274I	CTCGAG	1 cut(s)	2331
SfuI	TTCGAA	1 cut(s)	2149
SinI	GGWCC	3 cut(s)	704, 741, 2749
SlaI	CTCGAG	1 cut(s)	2331
SmaI	CCCGGG	2 cut(s)	10, 174
SmiMI	CAYNNNNRTG	2 cut(s)	971, 1410
SmlI	CTYRAG	4 cut(s)	1842, 1980, 2331, 2882
SmoI	CTYRAG	4 cut(s)	1842, 1980, 2331, 2882
SpeI	ACTAGT	2 cut(s)	1151, 1996
SseBI	AGGCCT	2 cut(s)	101, 1794
SsiI	CCGC	5 cut(s)	158, 166, 189, 744, 1863
SspMI	CTAG	6 cut(s)	1152, 1193, 1796, 1997, 2033, 2766
SstI	GAGCTC	1 cut(s)	424
StuI	AGGCCT	2 cut(s)	101, 1794
StyI	CCWWGG	6 cut(s)	870, 1027, 1059, 2128, 2418, 2783
TaaI	ACNGT	9 cut(s)	382, 594, 850, 1483, 1930, 2021, 2167, 2440, 2509
TaiI	ACGT	5 cut(s)	59, 130, 201, 368, 1705
TaqI	TCGA	9 cut(s)	596, 720, 729, 1563, 1655, 1725, 2149, 2332, 2601
TaqII	GACCGA	2 cut(s)	1078, 1750
TatI	WGTACW	2 cut(s)	845, 1591
TauI	GCSGC	2 cut(s)	169, 191
TfiI	GAWTC	6 cut(s)	386, 454, 731, 946, 2206, 2730
TscAI	CASTG	9 cut(s)	385, 978, 1272, 1449, 1638, 1947, 2026, 2322, 2512
TseFI	GTSAC	3 cut(s)	3, 1444, 2387
TseI	GCWGC	9 cut(s)	34, 131, 1073, 2003, 2555, 2606, 2741, 2798, 2807
Tsp45I	GTSAC	3 cut(s)	3, 1444, 2387
TspGWI	ACGGA	1 cut(s)	2806
TspMI	CCCGGG	2 cut(s)	8, 172
TspRI	CASTG	9 cut(s)	385, 978, 1272, 1449, 1638, 1947, 2026, 2322, 2512
Tth111I	GACNNNGTC	1 cut(s)	1401
VpaK11BI	GGWCC	3 cut(s)	704, 741, 2749
XapI	RAATTY	3 cut(s)	821, 1985, 2083
XbaI	TCTAGA	1 cut(s)	1192
XceI	RCATGY	1 cut(s)	1871
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2044
XhoI	CTCGAG	1 cut(s)	2331
XmaI	CCCGGG	2 cut(s)	8, 172
XmiI	GTMKAC	2 cut(s)	1092, 1849
XmnI	GAANNNNTTC	1 cut(s)	627
XspI	CTAG	6 cut(s)	1152, 1193, 1796, 1997, 2033, 2766
Zsp2I	ATGCAT	1 cut(s)	1873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.