Rmu_sc0000309.1_g000056

SecE/Sec61-gamma subunits of protein translocation complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000309.1
OG0003022
Physical Location & Seq
Forward (+)
296892 .. 299723
2832 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 672 bp
atggttcggttgctgtggaaacaaacggcctatggctttgttattcatgatgcacatggttctccgctcttggctggtgctagaagtgttggtaataccattagcccaaatactgaagtcctactgcactcgttggctttccattttcatccctcaaactctgagcgcgcaatccagagcgaccaagtgagcaggatggcggctgttttgcagtccgtggcaacatgttcaggtttgattcaccatccaggtagtgctaaactaccatttgggaataaggggaccaagatcactaatcacagtgccgtttcagtaacatgtagaaggacttgtagagcagggattcaatgtttggctgtacgtcgggttggcaccacatatgctgcgtctgtgggaaaggatcatcagcagttgagtattgatgatggagtacctcaggagcccttccttctgagtcttttcaaagaaatagtgtggtctttgagatcattattcatttttctgcttgagcagccaagtcagctgaagtacattgaatggccaggtttccgtagtacgctgaagactgcaactctcactcttgttcttgtggcattgctcattgtagcactttcatcaactgactctgccctcagctatttgttggctttgattctcaggagtaaaccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SecE

Protein Analysis

223

Amino Acids

24.3

Weight (kDa)

9.73

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecE PF00584 167 - 219 3.7e-06 SecE/Sec61-gamma subunits of protein translocation complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 67
AccII CGCG 1 cut(s) 168
AciI CCGC 2 cut(s) 65, 200
AclWI GGATC 1 cut(s) 408
AcoI YGGCCR 1 cut(s) 539
AcuI CTGAAG 3 cut(s) 135, 545, 581
AfaI GTAC 4 cut(s) 360, 432, 530, 556
AflIII ACRYGT 2 cut(s) 224, 317
AgsI TTSAA 3 cut(s) 347, 463, 536
AjnI CCWGG 2 cut(s) 247, 541
AluBI AGCT 2 cut(s) 523, 636
AluI AGCT 2 cut(s) 523, 636
AlwI GGATC 1 cut(s) 408
AoxI GGCC 2 cut(s) 27, 539
ApeKI GCWGC 2 cut(s) 383, 511
AspLEI GCGC 2 cut(s) 168, 170
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 282
AxyI CCTNAGG 1 cut(s) 435
BalI TGGCCA 1 cut(s) 541
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 569
BbvCI CCTCAGC 1 cut(s) 632
BbvI GCAGC 2 cut(s) 370, 523
BccI CCATC 3 cut(s) 190, 252, 419
BceAI ACGGC 2 cut(s) 42, 290
BciT130I CCWGG 2 cut(s) 249, 543
BfaI CTAG 1 cut(s) 81
BisI GCNGC 3 cut(s) 201, 384, 512
BlsI GCNGC 3 cut(s) 202, 385, 513
Bme1390I CCNGG 2 cut(s) 249, 543
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 3 cut(s) 283, 373, 441
BmrFI CCNGG 2 cut(s) 249, 543
BmsI GCATC 1 cut(s) 40
BpiI GAAGAC 1 cut(s) 569
Bpu10I CCTNAGC 1 cut(s) 632
BpuEI CTTGAG 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 216
Bse21I CCTNAGG 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 593
BseBI CCWGG 2 cut(s) 249, 543
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 3 cut(s) 148, 201, 244
BseMI GCAATG 1 cut(s) 593
BseMII CTCAG 5 cut(s) 153, 443, 449, 646, 670
BsePI GCGCGC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 370, 523
BsgI GTGCAG 1 cut(s) 110
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 29, 541
BshNI GGYRCC 1 cut(s) 371
BslFI GGGAC 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 2 cut(s) 29, 541
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 3 cut(s) 288, 400, 485
BspACI CCGC 2 cut(s) 65, 200
BspANI GGCC 2 cut(s) 29, 541
BspCNI CTCAG 5 cut(s) 154, 444, 448, 645, 669
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 283, 373, 441
BspPI GGATC 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 216
BssHII GCGCGC 1 cut(s) 166
BssMI GATC 3 cut(s) 288, 400, 485
Bst2UI CCWGG 2 cut(s) 249, 543
Bst4CI ACNGT 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 5 cut(s) 162, 435, 452, 632, 656
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 3 cut(s) 148, 201, 244
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 2 cut(s) 168, 170
BstKTI GATC 3 cut(s) 291, 403, 488
BstMBI GATC 3 cut(s) 288, 400, 485
BstMWI GCNNNNNNNGC 2 cut(s) 511, 520
BstNI CCWGG 2 cut(s) 249, 543
BstNSI RCATGY 2 cut(s) 228, 321
BstSCI CCNGG 2 cut(s) 247, 541
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 370, 523
BstV2I GAAGAC 1 cut(s) 569
Bsu36I CCTNAGG 1 cut(s) 435
BsuRI GGCC 2 cut(s) 29, 541
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 3 cut(s) 148, 201, 244
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 168
CciI TCATGA 1 cut(s) 46
CfoI GCGC 2 cut(s) 168, 170
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 1 cut(s) 375
Csp6I GTAC 4 cut(s) 359, 431, 529, 555
CviAII CATG 4 cut(s) 47, 56, 225, 318
CviQI GTAC 4 cut(s) 359, 431, 529, 555
DdeI CTNAG 5 cut(s) 162, 435, 452, 632, 656
DpnI GATC 3 cut(s) 290, 402, 487
DpnII GATC 3 cut(s) 288, 400, 485
EaeI YGGCCR 1 cut(s) 539
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 3 cut(s) 135, 545, 581
Eco81I CCTNAGG 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 247, 541
EcoT38I GRGCYC 1 cut(s) 444
FaeI CATG 4 cut(s) 50, 59, 228, 321
FaiI YATR 8 cut(s) 33, 48, 57, 226, 319, 379, 381, 670
FaqI GGGAC 1 cut(s) 295
FatI CATG 4 cut(s) 46, 55, 224, 317
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 3 cut(s) 201, 384, 512
FokI GGATG 3 cut(s) 135, 208, 231
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 3 cut(s) 201, 384, 512
FspBI CTAG 1 cut(s) 81
GlaI GCGC 2 cut(s) 167, 169
GluI GCNGC 3 cut(s) 201, 384, 512
HaeIII GGCC 2 cut(s) 29, 541
HgaI GACGC 1 cut(s) 375
HhaI GCGC 2 cut(s) 168, 170
Hin1II CATG 4 cut(s) 50, 59, 228, 321
Hin6I GCGC 2 cut(s) 166, 168
HinP1I GCGC 2 cut(s) 166, 168
HinfI GANTC 5 cut(s) 238, 343, 454, 623, 652
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 2 cut(s) 163, 453
Hpy188III TCNNGA 4 cut(s) 47, 175, 437, 658
Hpy8I GTNNAC 1 cut(s) 665
Hpy99I CGWCG 1 cut(s) 366
HpyAV CCTTC 3 cut(s) 318, 454, 458
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 53, 127, 211, 569
HpyF10VI GCNNNNNNNGC 2 cut(s) 511, 520
HpyF3I CTNAG 5 cut(s) 162, 435, 452, 632, 656
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 4 cut(s) 50, 59, 228, 321
HspAI GCGC 2 cut(s) 166, 168
Kzo9I GATC 3 cut(s) 288, 400, 485
LmnI GCTCC 1 cut(s) 439
Lsp1109I GCAGC 2 cut(s) 370, 523
LweI GCATC 1 cut(s) 40
MaeI CTAG 1 cut(s) 81
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 1 cut(s) 313
MalI GATC 3 cut(s) 290, 402, 487
MbiI CCGCTC 1 cut(s) 67
MboI GATC 3 cut(s) 288, 400, 485
MboII GAAGA 1 cut(s) 574
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 1 cut(s) 541
MluNI TGGCCA 1 cut(s) 541
MlyI GAGTC 2 cut(s) 463, 617
MnlI CCTC 3 cut(s) 163, 444, 641
Mox20I TGGCCA 1 cut(s) 541
MscI TGGCCA 1 cut(s) 541
Msp20I TGGCCA 1 cut(s) 541
MspA1I CMGCKG 1 cut(s) 523
MspR9I CCNGG 2 cut(s) 249, 543
MvaI CCWGG 2 cut(s) 249, 543
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 511, 520
NdeI CATATG 1 cut(s) 379
NdeII GATC 3 cut(s) 288, 400, 485
NlaIII CATG 4 cut(s) 50, 59, 228, 321
NlaIV GGNNCC 3 cut(s) 283, 373, 441
NspI RCATGY 2 cut(s) 228, 321
PagI TCATGA 1 cut(s) 46
PauI GCGCGC 1 cut(s) 166
PciI ACATGT 2 cut(s) 224, 317
PfeI GAWTC 3 cut(s) 238, 343, 652
PkrI GCNGC 3 cut(s) 202, 385, 513
PleI GAGTC 2 cut(s) 462, 617
PpsI GAGTC 2 cut(s) 462, 617
PscI ACATGT 2 cut(s) 224, 317
Psp6I CCWGG 2 cut(s) 247, 541
PspGI CCWGG 2 cut(s) 247, 541
PspN4I GGNNCC 3 cut(s) 283, 373, 441
PspPI GGNCC 1 cut(s) 282
PteI GCGCGC 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 4 cut(s) 360, 432, 530, 556
RsaNI GTAC 4 cut(s) 359, 431, 529, 555
SatI GCNGC 3 cut(s) 201, 384, 512
Sau3AI GATC 3 cut(s) 288, 400, 485
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 2 cut(s) 463, 617
ScrFI CCNGG 2 cut(s) 249, 543
SduI GDGCHC 1 cut(s) 444
SetI ASST 7 cut(s) 235, 253, 364, 436, 525, 547, 638
SfaNI GCATC 1 cut(s) 40
SinI GGWCC 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SsiI CCGC 2 cut(s) 65, 200
SspMI CTAG 1 cut(s) 81
StyD4I CCNGG 2 cut(s) 247, 541
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 364
TatI WGTACW 1 cut(s) 528
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 3 cut(s) 238, 343, 652
TscAI CASTG 1 cut(s) 307
TseI GCWGC 2 cut(s) 383, 511
TspDTI ATGAA 4 cut(s) 35, 137, 484, 603
TspGWI ACGGA 2 cut(s) 205, 539
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 1 cut(s) 282
XceI RCATGY 2 cut(s) 228, 321
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.