Rmu_sc0000573.1_g000018
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000573.1
OG0002466
Physical Location & Seq
Reverse (-)
71376 .. 73487
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2112 bp
atgagttgtgctggaatagtccccagaaagattcttattgaaactttgaaagcaaaacaacaaaatgaagagggaatagatcatcagaagatgatggctttgttctgttttctgcttctgcttctttttcactctctaaattttacttcagctgcttctaatcaggatgtggtgaagaagcttttgttgcagttcaagcaatcctctgttcaatctgaccctcatggtttcttatctgactggaaatctgattcttcaactgccctttgttcatggaaaggcctgacttgctctgaaggccacgtcatcaccctcgacctctcctattctggtctcattggaagtcttcaccttccaactctcacagccttgccaagtcttcagaatctgtacttgcaaggcaattcattctctgcttctgatctttcagtttccaacattaccacatgtagtcttgtaacaattgacttgtcctccaacaacatcacaagccctcttccggttcggtcttttcttgagggttgtgagaatctggctttggtcaacctctctggcaattcaatcccaggagggagtttcagctttggtgcttccttgcttcagcttgacatctctagaaacctgatttcggattcatccttgttgacttgccagaatctgaatctgctcaatgtttccggtaacaagctgaccggaaaactgagtgattctatcttgtcttgcaagaatctgacaacacttgacctttcatacaatgctttatctggggagatacccaacactttcctggaaagtggctctgcatctctcaagtatttggatctctcaagcaacaatttcactggaaagtttgcaagccttgatttcgggcagtgtagcagcctcacgttgctgaagctatcacacaataatctctatggggatgagtttccttcaagccttgctaactgtcaagctttggaaacactgaacctgactagtaataagttgcaggacaagattccgggtgctttgttgggaaatctcaagaagttgaggcaactctttctgggtcgcaatcagttttcgggtgaaattccagccgaattaggaaaggcttgtgggacacttcaggagcttgatatttctgataacattcttactggtgaattgccttcaagttttgtgtcgtgctcttctttggttagtctcaatcttggtcgcaatcagctctcaggaaatttcctcaatactgttgtgagtaagcttccgagtttgagatatctctatgtacccttcaacaacatcactggtcctgtgccaccatctattaccaatggtactcggcttcaagtgctcgatctcagcgctaatctcttcacagggagtgttccttcagtgttttgcttctccaatgctccctcagctttggaaaagatacttctagccaacaattttctctccgggactgtgccatcggaacttggaaactgcaagagcctgaaggctattgatctgagtttcaacagtttgagtggtgcaattcctttagaagtttggaccttgccaaatctttccgacttggttatgtgggcaaacaatctcactggtgaaatcccagaaggcatttgcatcaatggaggaaacctggaaaccctgattctcaacaataatctcattactggagccattccacagtcaattgccaactgcaccaatatgatttgggtgtcactatctagcaaccggcttacaggggcaatcccttcaggtattggaaatctccttaagcttgttatacttcagttgggtaataattcactgtctggtcagattccagctgagcttggcaagtgtcaaagcctcatttggcttgacctgaacagcaatgacctaaatggttctatcccatcagagcttgctaaccaggccggcctagtcattcctggaacagtttctggggagcagtttgcatttgttagaaatgagggtggtacagcttgcaggggtgcaggaggactagttgagtttgagggagtacgacctcagagactagaaagtttacctatggttcattcttgtccaacaactagaatttacacaggaatgacaaagacctccagcagaatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

703

Amino Acids

74.94

Weight (kDa)

5.52

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 61 - 98 4.1e-09 Leucine rich repeat N-terminal domain
LRR_4 PF12799 242 - 287 5.4e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 312 - 401 8.7e-07 Leucine-rich repeat region
LRR_8 PF13855 369 - 429 4.8e-08 Leucine rich repeat
Island PF20141 638 - 699 6.4e-28 Brassinosteroid receptor island domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 828
AcsI RAATTY 4 cut(s) 139, 1074, 1219, 2073
AfaI GTAC 5 cut(s) 392, 1272, 1321, 1975, 2019
AfeI AGCGCT 1 cut(s) 1348
AfiI CCNNNNNNNGG 2 cut(s) 499, 628
AflII CTTAAG 1 cut(s) 1766
AflIII ACRYGT 1 cut(s) 446
AhlI ACTAGT 2 cut(s) 977, 1999
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 5 cut(s) 565, 786, 1626, 1905, 1924
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
Alw21I GWGCWC 2 cut(s) 1175, 1338
Alw26I GTCTC 3 cut(s) 338, 1193, 2023
AlwI GGATC 1 cut(s) 828
AlwNI CAGNNNCTG 4 cut(s) 388, 658, 1937, 2109
Aor51HI AGCGCT 1 cut(s) 1348
AoxI GGCC 4 cut(s) 280, 298, 1908, 1912
ApeKI GCWGC 2 cut(s) 152, 879
ApoI RAATTY 4 cut(s) 139, 1074, 1219, 2073
Asp700I GAANNNNTTC 1 cut(s) 1933
AspLEI GCGC 1 cut(s) 1349
AspS9I GGNCC 2 cut(s) 1292, 1539
AsuC2I CCSGG 2 cut(s) 1005, 1444
AsuHPI GGTGA 6 cut(s) 184, 301, 341, 1082, 1157, 1601
AvaII GGWCC 2 cut(s) 1292, 1539
BbsI GAAGAC 2 cut(s) 338, 371
Bbv12I GWGCWC 2 cut(s) 1175, 1338
BbvCI CCTCAGC 1 cut(s) 1402
BbvI GCAGC 2 cut(s) 139, 891
BccI CCATC 4 cut(s) 88, 1312, 1462, 1897
BciT130I CCWGG 5 cut(s) 567, 788, 1628, 1907, 1926
BcnI CCSGG 2 cut(s) 1005, 1444
BcoDI GTCTC 3 cut(s) 338, 1193, 2023
BcuI ACTAGT 2 cut(s) 977, 1999
BfaI CTAG 8 cut(s) 615, 978, 1424, 1719, 1916, 2000, 2033, 2070
BfoI RGCGCY 1 cut(s) 1350
BfrI CTTAAG 1 cut(s) 1766
BisI GCNGC 2 cut(s) 153, 880
BlpI GCTNAGC 1 cut(s) 1821
BlsI GCNGC 2 cut(s) 154, 881
Bme1390I CCNGG 7 cut(s) 567, 788, 1005, 1444, 1628, 1907, 1926
Bme18I GGWCC 2 cut(s) 1292, 1539
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 2 cut(s) 1292, 1539
BmiI GGNNCC 1 cut(s) 1666
BmrFI CCNGG 7 cut(s) 567, 788, 1005, 1444, 1628, 1907, 1926
BmsI GCATC 2 cut(s) 812, 1620
BpiI GAAGAC 2 cut(s) 338, 371
BpmI CTGGAG 2 cut(s) 1683, 2083
Bpu10I CCTNAGC 1 cut(s) 1402
Bpu1102I GCTNAGC 1 cut(s) 1821
BpuEI CTTGAG 4 cut(s) 536, 794, 811, 1010
BpuMI CCSGG 2 cut(s) 1005, 1444
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 565
BsaWI WCCGGW 3 cut(s) 499, 677, 692
BsaXI ACNNNNNCTCC 4 cut(s) 458, 488, 1986, 2016
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 628
Bse118I RCCGGY 2 cut(s) 1725, 1910
Bse1I ACTGG 6 cut(s) 245, 847, 1147, 1294, 1591, 1666
Bse3DI GCAATG 1 cut(s) 1873
BseBI CCWGG 5 cut(s) 567, 788, 1628, 1907, 1926
BseDI CCNNGG 1 cut(s) 565
BseGI GGATG 3 cut(s) 172, 635, 928
BseLI CCNNNNNNNGG 2 cut(s) 499, 628
BseMI GCAATG 1 cut(s) 1873
BseMII CTCAG 7 cut(s) 692, 1227, 1357, 1416, 1487, 1812, 2039
BseNI ACTGG 6 cut(s) 245, 847, 1147, 1294, 1591, 1666
BseXI GCAGC 2 cut(s) 139, 891
BsgI GTGCAG 2 cut(s) 1675, 2010
BshFI GGCC 4 cut(s) 282, 300, 1910, 1914
BsiHKAI GWGCWC 2 cut(s) 1175, 1338
BsiSI CCGG 7 cut(s) 500, 678, 693, 1004, 1443, 1726, 1911
BslFI GGGAC 3 cut(s) 5, 1117, 1459
BslI CCNNNNNNNGG 2 cut(s) 499, 628
BsmAI GTCTC 3 cut(s) 338, 1193, 2023
BsmFI GGGAC 3 cut(s) 5, 1117, 1459
BsnI GGCC 4 cut(s) 282, 300, 1910, 1914
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 1175, 1338
Bsp143I GATC 5 cut(s) 79, 421, 820, 1339, 1492
Bsp1720I GCTNAGC 1 cut(s) 1821
BspANI GGCC 4 cut(s) 282, 300, 1910, 1914
BspCNI CTCAG 7 cut(s) 693, 1226, 1356, 1415, 1488, 1813, 2038
BspLI GGNNCC 1 cut(s) 1666
BspPI GGATC 1 cut(s) 828
BspQI GCTCTTC 1 cut(s) 1180
BspTI CTTAAG 1 cut(s) 1766
BspTNI GGTCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 1873
BsrFI RCCGGY 2 cut(s) 1725, 1910
BsrI ACTGG 6 cut(s) 245, 847, 1147, 1294, 1591, 1666
BssAI RCCGGY 2 cut(s) 1725, 1910
BssECI CCNNGG 1 cut(s) 565
BssMI GATC 5 cut(s) 79, 421, 820, 1339, 1492
Bst2UI CCWGG 5 cut(s) 567, 788, 1628, 1907, 1926
Bst4CI ACNGT 7 cut(s) 950, 1234, 1450, 1508, 1677, 1803, 1933
Bst6I CTCTTC 4 cut(s) 63, 501, 1180, 1361
BstAFI CTTAAG 1 cut(s) 1766
BstC8I GCNNGC 4 cut(s) 856, 1899, 1912, 1981
BstDEI CTNAG 7 cut(s) 701, 1213, 1343, 1402, 1496, 1821, 2025
BstF5I GGATG 3 cut(s) 172, 635, 928
BstH2I RGCGCY 1 cut(s) 1350
BstHHI GCGC 1 cut(s) 1349
BstKTI GATC 5 cut(s) 82, 424, 823, 1342, 1495
BstMAI GTCTC 3 cut(s) 338, 1193, 2023
BstMBI GATC 5 cut(s) 79, 421, 820, 1339, 1492
BstMWI GCNNNNNNNGC 7 cut(s) 187, 196, 288, 297, 1333, 1403, 1907
BstNI CCWGG 5 cut(s) 567, 788, 1628, 1907, 1926
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 7 cut(s) 565, 786, 1003, 1442, 1626, 1905, 1924
BstV1I GCAGC 2 cut(s) 139, 891
BstV2I GAAGAC 2 cut(s) 338, 371
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
BsuRI GGCC 4 cut(s) 282, 300, 1910, 1914
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 3 cut(s) 172, 635, 928
BtsI GCAGTG 1 cut(s) 878
BtsIMutI CAGTG 7 cut(s) 840, 878, 965, 1287, 1383, 1584, 1799
Cac8I GCNNGC 4 cut(s) 856, 1899, 1912, 1981
CaiI CAGNNNCTG 4 cut(s) 388, 658, 1937, 2109
CfoI GCGC 1 cut(s) 1349
Cfr10I RCCGGY 2 cut(s) 1725, 1910
Cfr13I GGNCC 2 cut(s) 1292, 1539
Csp6I GTAC 5 cut(s) 391, 1271, 1320, 1974, 2018
CviAII CATG 3 cut(s) 224, 273, 447
CviQI GTAC 5 cut(s) 391, 1271, 1320, 1974, 2018
DdeI CTNAG 7 cut(s) 701, 1213, 1343, 1402, 1496, 1821, 2025
DpnI GATC 5 cut(s) 81, 423, 822, 1341, 1494
DpnII GATC 5 cut(s) 79, 421, 820, 1339, 1492
Eam1104I CTCTTC 4 cut(s) 63, 501, 1180, 1361
EarI CTCTTC 4 cut(s) 63, 501, 1180, 1361
Eco147I AGGCCT 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 1262
Eco47I GGWCC 2 cut(s) 1292, 1539
Eco47III AGCGCT 1 cut(s) 1348
EcoRII CCWGG 5 cut(s) 565, 786, 1626, 1905, 1924
EcoRV GATATC 1 cut(s) 1262
FaeI CATG 3 cut(s) 227, 276, 450
FaqI GGGAC 3 cut(s) 5, 1117, 1459
FatI CATG 3 cut(s) 223, 272, 446
Fnu4HI GCNGC 2 cut(s) 153, 880
FokI GGATG 3 cut(s) 179, 622, 935
FseI GGCCGGCC 1 cut(s) 1914
Fsp4HI GCNGC 2 cut(s) 153, 880
FspBI CTAG 8 cut(s) 615, 978, 1424, 1719, 1916, 2000, 2033, 2070
GlaI GCGC 1 cut(s) 1348
GluI GCNGC 2 cut(s) 153, 880
GsuI CTGGAG 2 cut(s) 1683, 2083
HaeII RGCGCY 1 cut(s) 1350
HaeIII GGCC 4 cut(s) 282, 300, 1910, 1914
HapII CCGG 7 cut(s) 500, 678, 693, 1004, 1443, 1726, 1911
HhaI GCGC 1 cut(s) 1349
Hin1II CATG 3 cut(s) 227, 276, 450
Hin6I GCGC 1 cut(s) 1347
HinP1I GCGC 1 cut(s) 1347
HincII GTYRAC 2 cut(s) 544, 645
HindII GTYRAC 2 cut(s) 544, 645
HindIII AAGCTT 4 cut(s) 179, 954, 1244, 1769
HpaII CCGG 7 cut(s) 500, 678, 693, 1004, 1443, 1726, 1911
HphI GGTGA 6 cut(s) 184, 301, 341, 1082, 1157, 1601
Hpy166II GTNNAC 3 cut(s) 544, 645, 2042
Hpy188III TCNNGA 6 cut(s) 164, 515, 615, 1027, 1112, 1215
Hpy8I GTNNAC 3 cut(s) 544, 645, 2042
HpyAV CCTTC 9 cut(s) 290, 362, 942, 1164, 1285, 1383, 1477, 1595, 1755
HpyCH4III ACNGT 7 cut(s) 950, 1234, 1450, 1508, 1677, 1803, 1933
HpyCH4IV ACGT 2 cut(s) 303, 887
HpyF10VI GCNNNNNNNGC 7 cut(s) 187, 196, 288, 297, 1333, 1403, 1907
HpyF3I CTNAG 7 cut(s) 701, 1213, 1343, 1402, 1496, 1821, 2025
HpySE526I ACGT 2 cut(s) 303, 887
Hsp92II CATG 3 cut(s) 227, 276, 450
HspAI GCGC 1 cut(s) 1347
KroI GCCGGC 1 cut(s) 1910
KroNI GCCGGC 1 cut(s) 1912
Kzo9I GATC 5 cut(s) 79, 421, 820, 1339, 1492
LguI GCTCTTC 1 cut(s) 1180
LmnI GCTCC 4 cut(s) 1114, 1402, 1664, 1942
Lsp1109I GCAGC 2 cut(s) 139, 891
LweI GCATC 2 cut(s) 812, 1620
MaeI CTAG 8 cut(s) 615, 978, 1424, 1719, 1916, 2000, 2033, 2070
MaeII ACGT 2 cut(s) 303, 887
MaeIII GTNAC 3 cut(s) 457, 680, 1710
MalI GATC 5 cut(s) 81, 423, 822, 1341, 1494
MboI GATC 5 cut(s) 79, 421, 820, 1339, 1492
MboII GAAGA 9 cut(s) 80, 100, 187, 246, 338, 371, 488, 1167, 1348
MfeI CAATTG 2 cut(s) 462, 1680
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 2 cut(s) 1175, 1338
MmeI TCCRAC 5 cut(s) 380, 459, 501, 1581, 2087
MroNI GCCGGC 1 cut(s) 1910
MroXI GAANNNNTTC 1 cut(s) 1933
MseI TTAA 1 cut(s) 1767
MslI CAYNNNNRTG 2 cut(s) 1697, 2084
MspA1I CMGCKG 2 cut(s) 152, 1820
MspCI CTTAAG 1 cut(s) 1766
MspI CCGG 7 cut(s) 500, 678, 693, 1004, 1443, 1726, 1911
MspR9I CCNGG 7 cut(s) 567, 788, 1005, 1444, 1628, 1907, 1926
MunI CAATTG 2 cut(s) 462, 1680
MvaI CCWGG 5 cut(s) 567, 788, 1628, 1907, 1926
MwoI GCNNNNNNNGC 7 cut(s) 187, 196, 288, 297, 1333, 1403, 1907
NaeI GCCGGC 1 cut(s) 1912
NciI CCSGG 2 cut(s) 1005, 1444
NdeII GATC 5 cut(s) 79, 421, 820, 1339, 1492
NgoMIV GCCGGC 1 cut(s) 1910
NlaIII CATG 3 cut(s) 227, 276, 450
NlaIV GGNNCC 1 cut(s) 1666
NmeAIII GCCGAG 1 cut(s) 1303
NmuCI GTSAC 1 cut(s) 1710
NspI RCATGY 1 cut(s) 450
PceI AGGCCT 1 cut(s) 282
PciI ACATGT 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 1180
PdiI GCCGGC 1 cut(s) 1912
PdmI GAANNNNTTC 1 cut(s) 1933
PfoI TCCNGGA 3 cut(s) 786, 1442, 1924
PkrI GCNGC 2 cut(s) 154, 881
PscI ACATGT 1 cut(s) 446
Psp6I CCWGG 5 cut(s) 565, 786, 1626, 1905, 1924
PspGI CCWGG 5 cut(s) 565, 786, 1626, 1905, 1924
PspN4I GGNNCC 1 cut(s) 1666
PspPI GGNCC 2 cut(s) 1292, 1539
PstNI CAGNNNCTG 4 cut(s) 388, 658, 1937, 2109
PsuI RGATCY 1 cut(s) 820
PvuII CAGCTG 2 cut(s) 152, 1820
RigI GGCCGGCC 1 cut(s) 1914
RsaI GTAC 5 cut(s) 392, 1272, 1321, 1975, 2019
RsaNI GTAC 5 cut(s) 391, 1271, 1320, 1974, 2018
RseI CAYNNNNRTG 2 cut(s) 1697, 2084
SapI GCTCTTC 1 cut(s) 1180
SaqAI TTAA 1 cut(s) 1767
SatI GCNGC 2 cut(s) 153, 880
Sau3AI GATC 5 cut(s) 79, 421, 820, 1339, 1492
Sau96I GGNCC 2 cut(s) 1292, 1539
ScrFI CCNGG 7 cut(s) 567, 788, 1005, 1444, 1628, 1907, 1926
SduI GDGCHC 2 cut(s) 1175, 1338
SfaNI GCATC 2 cut(s) 812, 1620
SinI GGWCC 2 cut(s) 1292, 1539
SmiMI CAYNNNNRTG 2 cut(s) 1697, 2084
SmlI CTYRAG 5 cut(s) 515, 809, 826, 1025, 1766
SmoI CTYRAG 5 cut(s) 515, 809, 826, 1025, 1766
SpeI ACTAGT 2 cut(s) 977, 1999
SseBI AGGCCT 1 cut(s) 282
SspMI CTAG 8 cut(s) 615, 978, 1424, 1719, 1916, 2000, 2033, 2070
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 7 cut(s) 565, 786, 1003, 1442, 1626, 1905, 1924
TaaI ACNGT 7 cut(s) 950, 1234, 1450, 1508, 1677, 1803, 1933
TaiI ACGT 2 cut(s) 306, 890
TaqI TCGA 2 cut(s) 315, 1338
TaqII GACCGA 1 cut(s) 495
TatI WGTACW 1 cut(s) 390
Tru1I TTAA 1 cut(s) 1767
Tru9I TTAA 1 cut(s) 1767
TscAI CASTG 7 cut(s) 847, 878, 972, 1294, 1383, 1591, 1806
TseFI GTSAC 1 cut(s) 1710
TseI GCWGC 2 cut(s) 152, 879
Tsp45I GTSAC 1 cut(s) 1710
TspDTI ATGAA 6 cut(s) 81, 261, 396, 624, 738, 2042
TspRI CASTG 7 cut(s) 847, 878, 972, 1294, 1383, 1591, 1806
Vha464I CTTAAG 1 cut(s) 1766
VpaK11BI GGWCC 2 cut(s) 1292, 1539
XapI RAATTY 4 cut(s) 139, 1074, 1219, 2073
XbaI TCTAGA 1 cut(s) 614
XceI RCATGY 1 cut(s) 450
XcmI CCANNNNNNNNNTGG 1 cut(s) 784
XmnI GAANNNNTTC 1 cut(s) 1933
XspI CTAG 8 cut(s) 615, 978, 1424, 1719, 1916, 2000, 2033, 2070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.