Rmu_sc0001708.1_g000047

polyadenylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001708.1
OG0001968
Physical Location & Seq
Reverse (-)
186127 .. 189772
3646 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
atggccgaagaagagcatgaggtgtacggagcagaaattccatacgaggcggagatggacggcgacttcgacccctatcccgacgacgatggcatctccaccgcccacggcgacgacgccgccgtcaaggaggaggaagcttcggctctccacgagattcaggctgatggggcgaaggagatgggtgctgcgcaagatcctgctactgcagctgattctcaggcaaacaaggaagcggctgatgaatgttctgttttcgttggcaatgagagggttgcaaatgcctttgaagaggaaaggaaagatgggttcacgactgtagttgcagggcttctctgctgctggatgctgttgtttggtgcctatgtagcaaatgcggttgattatgcatgcacacctgaagaagtacagcagcatttcgaatcatgtggcacagttaacagagtgactatactgacagataaatttggccaaccaaagggttttgcctatgtggaattccttgaaacagaagctgttcaaaaggctcttgttctgaatgaatctgagttgcatggtcggcaattgaaggttttgcctaaaaggaccaatgttcctggatataagcaattccgccctagacgtttcaatccgtatatgggttactgcatcaggagcccttatgtacctccttacttctattcgccctatggatatgggaaagttcccaggttcagaaggccagcacgatacatgccctactag

Protein Analysis

247

Amino Acids

27.66

Weight (kDa)

4.7

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 128 - 190 1.2e-13 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
Acc16I TGCGCA 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 359
AciI CCGC 6 cut(s) 50, 102, 120, 236, 377, 613
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 2 cut(s) 3, 469
AcsI RAATTY 3 cut(s) 36, 464, 497
AcuI CTGAAG 1 cut(s) 420
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 3 cut(s) 26, 408, 666
AfiI CCNNNNNNNGG 2 cut(s) 478, 638
AgsI TTSAA 5 cut(s) 290, 506, 521, 568, 628
AjnI CCWGG 2 cut(s) 595, 707
AluBI AGCT 3 cut(s) 140, 212, 515
AluI AGCT 3 cut(s) 140, 212, 515
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 3 cut(s) 3, 469, 719
ApeKI GCWGC 4 cut(s) 188, 209, 339, 412
ApoI RAATTY 3 cut(s) 36, 464, 497
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp700I GAANNNNTTC 1 cut(s) 516
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 1 cut(s) 585
AsuII TTCGAA 1 cut(s) 420
AvaII GGWCC 1 cut(s) 585
BalI TGGCCA 1 cut(s) 471
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 1 cut(s) 659
BauI CACGAG 1 cut(s) 152
BbvI GCAGC 4 cut(s) 175, 221, 326, 424
BccI CCATC 5 cut(s) 49, 83, 161, 175, 299
BceAI ACGGC 3 cut(s) 76, 107, 124
BciT130I CCWGG 2 cut(s) 597, 709
BfaI CTAG 2 cut(s) 618, 742
BfmI CTRYAG 2 cut(s) 207, 318
BisI GCNGC 6 cut(s) 120, 189, 210, 237, 340, 413
BlsI GCNGC 6 cut(s) 121, 190, 211, 238, 341, 414
Bme1390I CCNGG 2 cut(s) 597, 709
Bme18I GGWCC 1 cut(s) 585
BmgT120I GGNCC 1 cut(s) 585
BmiI GGNNCC 2 cut(s) 361, 656
BmrFI CCNGG 2 cut(s) 597, 709
BmsI GCATC 3 cut(s) 102, 336, 657
Bpu14I TTCGAA 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 106, 707
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 638
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 597, 709
BseDI CCNNGG 2 cut(s) 106, 707
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 478, 638
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 233, 537
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 4 cut(s) 175, 221, 326, 424
BshFI GGCC 3 cut(s) 5, 471, 721
BshNI GGYRCC 1 cut(s) 359
BslI CCNNNNNNNGG 2 cut(s) 478, 638
BsnI GGCC 3 cut(s) 5, 471, 721
Bsp119I TTCGAA 1 cut(s) 420
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 1 cut(s) 196
BspACI CCGC 6 cut(s) 50, 102, 120, 236, 377, 613
BspANI GGCC 3 cut(s) 5, 471, 721
BspCNI CTCAG 2 cut(s) 232, 538
BspLI GGNNCC 2 cut(s) 361, 656
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 1 cut(s) 191
BspQI GCTCTTC 1 cut(s) 6
BspT104I TTCGAA 1 cut(s) 420
BspT107I GGYRCC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 106, 707
BssMI GATC 1 cut(s) 196
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 2 cut(s) 597, 709
Bst4CI ACNGT 2 cut(s) 319, 436
Bst6I CTCTTC 2 cut(s) 6, 285
BstACI GRCGYC 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 391, 723
BstDEI CTNAG 2 cut(s) 219, 546
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 351
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 1 cut(s) 199
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 5 cut(s) 170, 209, 368, 559, 654
BstNI CCWGG 2 cut(s) 597, 709
BstNSI RCATGY 2 cut(s) 393, 736
BstSCI CCNGG 2 cut(s) 595, 707
BstSFI CTRYAG 2 cut(s) 207, 318
BstV1I GCAGC 4 cut(s) 175, 221, 326, 424
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 3 cut(s) 5, 471, 721
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 391, 723
CaiI CAGNNNCTG 1 cut(s) 515
CfoI GCGC 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 585
CseI GACGC 1 cut(s) 125
Csp6I GTAC 3 cut(s) 25, 407, 665
CviAII CATG 5 cut(s) 17, 390, 426, 554, 733
CviQI GTAC 3 cut(s) 25, 407, 665
DdeI CTNAG 2 cut(s) 219, 546
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 2 cut(s) 3, 469
Eam1104I CTCTTC 2 cut(s) 6, 285
EarI CTCTTC 2 cut(s) 6, 285
EciI GGCGGA 2 cut(s) 65, 602
Eco24I GRGCYC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 497
EcoRII CCWGG 2 cut(s) 595, 707
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 659
FaeI CATG 5 cut(s) 20, 393, 429, 557, 736
FatI CATG 5 cut(s) 16, 389, 425, 553, 732
Fnu4HI GCNGC 6 cut(s) 120, 189, 210, 237, 340, 413
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 6 cut(s) 120, 189, 210, 237, 340, 413
FspBI CTAG 2 cut(s) 618, 742
FspI TGCGCA 1 cut(s) 192
GlaI GCGC 1 cut(s) 192
GluI GCNGC 6 cut(s) 120, 189, 210, 237, 340, 413
HaeIII GGCC 3 cut(s) 5, 471, 721
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 5 cut(s) 20, 393, 429, 557, 736
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 4 cut(s) 157, 215, 422, 542
HpaI GTTAAC 1 cut(s) 439
Hpy166II GTNNAC 3 cut(s) 25, 312, 439
Hpy188I TCNGA 3 cut(s) 537, 547, 716
Hpy188III TCNNGA 3 cut(s) 80, 313, 652
Hpy8I GTNNAC 3 cut(s) 25, 312, 439
Hpy99I CGWCG 4 cut(s) 86, 89, 116, 119
HpyAV CCTTC 3 cut(s) 169, 562, 711
HpyCH4III ACNGT 2 cut(s) 319, 436
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 7 cut(s) 209, 278, 326, 389, 393, 553, 648
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 209, 368, 559, 654
HpyF3I CTNAG 2 cut(s) 219, 546
HpySE526I ACGT 1 cut(s) 622
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 5 cut(s) 20, 393, 429, 557, 736
HspAI GCGC 1 cut(s) 191
KspAI GTTAAC 1 cut(s) 439
Kzo9I GATC 1 cut(s) 196
LguI GCTCTTC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 29, 654
Lsp1109I GCAGC 4 cut(s) 175, 221, 326, 424
LweI GCATC 3 cut(s) 102, 336, 657
MaeI CTAG 2 cut(s) 618, 742
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 2 cut(s) 445, 641
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 4 cut(s) 20, 23, 302, 413
MfeI CAATTG 1 cut(s) 563
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 5 cut(s) 36, 464, 497, 563, 608
MluNI TGGCCA 1 cut(s) 471
MnlI CCTC 7 cut(s) 13, 40, 124, 127, 264, 286, 678
Mox20I TGGCCA 1 cut(s) 471
Mph1103I ATGCAT 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 516
MscI TGGCCA 1 cut(s) 471
MseI TTAA 1 cut(s) 438
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 1 cut(s) 212
MspR9I CCNGG 2 cut(s) 597, 709
MunI CAATTG 1 cut(s) 563
MvaI CCWGG 2 cut(s) 597, 709
MwoI GCNNNNNNNGC 5 cut(s) 170, 209, 368, 559, 654
NdeII GATC 1 cut(s) 196
NlaIII CATG 5 cut(s) 20, 393, 429, 557, 736
NlaIV GGNNCC 2 cut(s) 361, 656
NmuCI GTSAC 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 393, 736
NspV TTCGAA 1 cut(s) 420
PaeI GCATGC 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 6
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 120
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 4 cut(s) 157, 215, 422, 542
PfoI TCCNGGA 1 cut(s) 595
PkrI GCNGC 6 cut(s) 121, 190, 211, 238, 341, 414
Psp6I CCWGG 2 cut(s) 595, 707
PspGI CCWGG 2 cut(s) 595, 707
PspN4I GGNNCC 2 cut(s) 361, 656
PspPI GGNCC 1 cut(s) 585
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 3 cut(s) 26, 408, 666
RsaNI GTAC 3 cut(s) 25, 407, 665
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 6 cut(s) 120, 189, 210, 237, 340, 413
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 1 cut(s) 585
ScrFI CCNGG 2 cut(s) 597, 709
SduI GDGCHC 1 cut(s) 659
SetI ASST 9 cut(s) 24, 142, 214, 400, 517, 573, 625, 670, 713
SfaNI GCATC 3 cut(s) 102, 336, 657
SfcI CTRYAG 2 cut(s) 207, 318
SfuI TTCGAA 1 cut(s) 420
SinI GGWCC 1 cut(s) 585
SphI GCATGC 1 cut(s) 393
Sse9I AATT 5 cut(s) 36, 464, 497, 563, 608
SsiI CCGC 6 cut(s) 50, 102, 120, 236, 377, 613
SspMI CTAG 2 cut(s) 618, 742
StyD4I CCNGG 2 cut(s) 595, 707
TaaI ACNGT 2 cut(s) 319, 436
TaiI ACGT 1 cut(s) 625
TaqI TCGA 2 cut(s) 69, 420
TasI AATT 5 cut(s) 36, 464, 497, 563, 608
TatI WGTACW 1 cut(s) 406
TauI GCSGC 2 cut(s) 122, 239
TfiI GAWTC 4 cut(s) 157, 215, 422, 542
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 4 cut(s) 188, 209, 339, 412
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 2 cut(s) 258, 555
TspGWI ACGGA 2 cut(s) 42, 621
VpaK11BI GGWCC 1 cut(s) 585
XapI RAATTY 3 cut(s) 36, 464, 497
XceI RCATGY 2 cut(s) 393, 736
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 2 cut(s) 618, 742
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.