Rw2G018260

polyadenylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001968
Physical Location & Seq
Forward (+)
23406593 .. 23410397
3805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018260.1

Sequence Viewer

Length: 636 bp
ATGGCCGAAGAAGAGCATGAGGTGTACGGAGCAGAAATTCCATACGAGGCGGAGATGGACGGCGACTTCGACCCCTATCCCGACGACGATGGCATCTCCACCGCCCACGGCGACGACGCCGCCGTCAAGGAGGAGGAAGCTTCGGCTCTCCACGAGATTCAGGCTGATGGGGCGAAGGAGATGGGTGCTGCGCAAGATCCTGCTACTGCAGCTGATTCTCAGGCAAACAAGGAAGCGGCTGATGAATGTTCTGTTTTCGTTGGCAATGTTGATTATGCATGCACACCTGAAGAAGTACAGCAGCATTTCCAATCATGTGGCACAGTTAACAGAGTGACTATACTGACAGATAAATTTGGCCAACCAAAGGGTTTTGCCTATGTGGAATTCCTTGAAACAGAAGCTGTTCAAAAGGCTCTTGTTCTGAATGAATCTGAGTTGCATGGTCGGCAATTGAAGGTTTTGCCTAAAAGGACCAATGTTCCTGGATATAAGCAATTCCGCCCTAGACGTTTCAATCCGTATATGGGTTACTGCATCAGGAGCCCTTATGTACCTCCTTACTTCTATTCGCCCTATGGATATGGGAAAGTTCCCAGGTTCAGAAGGCCAGCACGATACATGCCCTACAAGTAG

Protein Analysis

211

Amino Acids

23.71

Weight (kDa)

4.82

Isoelectric Point (pI)

56.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 85 - 153 5.4e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
Acc16I TGCGCA 1 cut(s) 192
AciI CCGC 5 cut(s) 50, 102, 120, 236, 502
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 2 cut(s) 3, 358
AcsI RAATTY 3 cut(s) 36, 353, 386
AcuI CTGAAG 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 3 cut(s) 26, 297, 555
AfiI CCNNNNNNNGG 2 cut(s) 367, 527
AgsI TTSAA 4 cut(s) 395, 410, 457, 517
AjnI CCWGG 2 cut(s) 484, 596
AluBI AGCT 3 cut(s) 140, 212, 404
AluI AGCT 3 cut(s) 140, 212, 404
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 3 cut(s) 3, 358, 608
ApeKI GCWGC 3 cut(s) 188, 209, 301
ApoI RAATTY 3 cut(s) 36, 353, 386
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 1 cut(s) 474
AvaII GGWCC 1 cut(s) 474
BalI TGGCCA 1 cut(s) 360
BanII GRGCYC 1 cut(s) 548
BauI CACGAG 1 cut(s) 152
BbvI GCAGC 3 cut(s) 175, 221, 313
BccI CCATC 4 cut(s) 49, 83, 161, 175
BceAI ACGGC 3 cut(s) 76, 107, 124
BciT130I CCWGG 2 cut(s) 486, 598
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 5 cut(s) 120, 189, 210, 237, 302
BlsI GCNGC 5 cut(s) 121, 190, 211, 238, 303
Bme1390I CCNGG 2 cut(s) 486, 598
Bme18I GGWCC 1 cut(s) 474
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 1 cut(s) 545
BmrFI CCNGG 2 cut(s) 486, 598
BmsI GCATC 2 cut(s) 102, 546
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 106, 596
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 527
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 486, 598
BseDI CCNNGG 2 cut(s) 106, 596
BseLI CCNNNNNNNGG 2 cut(s) 367, 527
BseMI GCAATG 1 cut(s) 271
BseMII CTCAG 2 cut(s) 233, 426
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 3 cut(s) 175, 221, 313
BshFI GGCC 3 cut(s) 5, 360, 610
BslI CCNNNNNNNGG 2 cut(s) 367, 527
BsnI GGCC 3 cut(s) 5, 360, 610
Bsp1286I GDGCHC 1 cut(s) 548
Bsp143I GATC 1 cut(s) 196
BspACI CCGC 5 cut(s) 50, 102, 120, 236, 502
BspANI GGCC 3 cut(s) 5, 360, 610
BspCNI CTCAG 2 cut(s) 232, 427
BspLI GGNNCC 1 cut(s) 545
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 1 cut(s) 191
BspQI GCTCTTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 106, 596
BssMI GATC 1 cut(s) 196
BssNI GRCGYC 1 cut(s) 117
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 2 cut(s) 486, 598
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 6
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 280, 612
BstDEI CTNAG 2 cut(s) 219, 435
BstDSI CCRYGG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 1 cut(s) 199
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 4 cut(s) 170, 209, 448, 543
BstNI CCWGG 2 cut(s) 486, 598
BstNSI RCATGY 2 cut(s) 282, 625
BstSCI CCNGG 2 cut(s) 484, 596
BstSFI CTRYAG 1 cut(s) 207
BstV1I GCAGC 3 cut(s) 175, 221, 313
BstX2I RGATCY 1 cut(s) 196
BstXI CCANNNNNNTGG 1 cut(s) 317
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 3 cut(s) 5, 360, 610
BtgI CCRYGG 1 cut(s) 106
Cac8I GCNNGC 2 cut(s) 280, 612
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 474
CseI GACGC 1 cut(s) 125
Csp6I GTAC 3 cut(s) 25, 296, 554
CviAII CATG 5 cut(s) 17, 279, 315, 443, 622
CviQI GTAC 3 cut(s) 25, 296, 554
DdeI CTNAG 2 cut(s) 219, 435
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 2 cut(s) 3, 358
Eam1104I CTCTTC 1 cut(s) 6
EarI CTCTTC 1 cut(s) 6
EciI GGCGGA 2 cut(s) 65, 491
Eco24I GRGCYC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 474
Eco57I CTGAAG 1 cut(s) 309
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 484, 596
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 548
FaeI CATG 5 cut(s) 20, 282, 318, 446, 625
FatI CATG 5 cut(s) 16, 278, 314, 442, 621
Fnu4HI GCNGC 5 cut(s) 120, 189, 210, 237, 302
FriOI GRGCYC 1 cut(s) 548
Fsp4HI GCNGC 5 cut(s) 120, 189, 210, 237, 302
FspBI CTAG 1 cut(s) 507
FspI TGCGCA 1 cut(s) 192
GlaI GCGC 1 cut(s) 192
GluI GCNGC 5 cut(s) 120, 189, 210, 237, 302
HaeIII GGCC 3 cut(s) 5, 360, 610
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 5 cut(s) 20, 282, 318, 446, 625
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 3 cut(s) 157, 215, 431
HpaI GTTAAC 1 cut(s) 328
Hpy166II GTNNAC 2 cut(s) 25, 328
Hpy188I TCNGA 3 cut(s) 426, 436, 605
Hpy188III TCNNGA 2 cut(s) 80, 541
Hpy8I GTNNAC 2 cut(s) 25, 328
Hpy99I CGWCG 4 cut(s) 86, 89, 116, 119
HpyAV CCTTC 3 cut(s) 169, 451, 600
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 5 cut(s) 209, 278, 282, 442, 537
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 209, 448, 543
HpyF3I CTNAG 2 cut(s) 219, 435
HpySE526I ACGT 1 cut(s) 511
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 5 cut(s) 20, 282, 318, 446, 625
HspAI GCGC 1 cut(s) 191
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 1 cut(s) 196
LguI GCTCTTC 1 cut(s) 6
LmnI GCTCC 2 cut(s) 29, 543
Lsp1109I GCAGC 3 cut(s) 175, 221, 313
LweI GCATC 2 cut(s) 102, 546
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 2 cut(s) 334, 530
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 3 cut(s) 20, 23, 302
MfeI CAATTG 1 cut(s) 452
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 1 cut(s) 548
MlsI TGGCCA 1 cut(s) 360
MluCI AATT 5 cut(s) 36, 353, 386, 452, 497
MluNI TGGCCA 1 cut(s) 360
MnlI CCTC 5 cut(s) 13, 40, 124, 127, 567
Mox20I TGGCCA 1 cut(s) 360
Mph1103I ATGCAT 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 1 cut(s) 360
MseI TTAA 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 212
MspR9I CCNGG 2 cut(s) 486, 598
MunI CAATTG 1 cut(s) 452
MvaI CCWGG 2 cut(s) 486, 598
MwoI GCNNNNNNNGC 4 cut(s) 170, 209, 448, 543
NdeII GATC 1 cut(s) 196
NlaIII CATG 5 cut(s) 20, 282, 318, 446, 625
NlaIV GGNNCC 1 cut(s) 545
NmuCI GTSAC 1 cut(s) 334
NsbI TGCGCA 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 2 cut(s) 282, 625
PaeI GCATGC 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 6
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 120
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 3 cut(s) 157, 215, 431
PfoI TCCNGGA 1 cut(s) 484
PkrI GCNGC 5 cut(s) 121, 190, 211, 238, 303
Psp6I CCWGG 2 cut(s) 484, 596
PspGI CCWGG 2 cut(s) 484, 596
PspN4I GGNNCC 1 cut(s) 545
PspPI GGNCC 1 cut(s) 474
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 3 cut(s) 26, 297, 555
RsaNI GTAC 3 cut(s) 25, 296, 554
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 5 cut(s) 120, 189, 210, 237, 302
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 1 cut(s) 474
ScrFI CCNGG 2 cut(s) 486, 598
SduI GDGCHC 1 cut(s) 548
SetI ASST 9 cut(s) 24, 142, 214, 289, 406, 462, 514, 559, 602
SfaNI GCATC 2 cut(s) 102, 546
SfcI CTRYAG 1 cut(s) 207
SinI GGWCC 1 cut(s) 474
SphI GCATGC 1 cut(s) 282
Sse9I AATT 5 cut(s) 36, 353, 386, 452, 497
SsiI CCGC 5 cut(s) 50, 102, 120, 236, 502
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 484, 596
TaaI ACNGT 1 cut(s) 325
TaiI ACGT 1 cut(s) 514
TaqI TCGA 1 cut(s) 69
TasI AATT 5 cut(s) 36, 353, 386, 452, 497
TatI WGTACW 1 cut(s) 295
TauI GCSGC 2 cut(s) 122, 239
TfiI GAWTC 3 cut(s) 157, 215, 431
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 188, 209, 301
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 258, 444
TspGWI ACGGA 2 cut(s) 42, 510
VpaK11BI GGWCC 1 cut(s) 474
XapI RAATTY 3 cut(s) 36, 353, 386
XceI RCATGY 2 cut(s) 282, 625
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 1 cut(s) 507
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.